Aldolase catalyzes a key reaction in glycolysis and energy production.
Stability/Storage: The enzyme is irreversibly denatured at pH values lower than 4.5. A crystalline suspension in ammonium sulfate solution, pH 7.6, is stable for at least six months at 2-8°C.
One Unit causes an increase of 1.0 A240 per minute at 25°C, pH 7.5 with the hydrazine/3-phosphoglyceraldehyde assay (Jagannathan et al., Biochem. J., 63 94, 1956).
Two times crystallized. A suspension in 2.1M ammonium sulfate, pH 7.8.
Store at 2-8°C.
≥10 units per mg protein
Chromatographically purified. An inactive lyophilized powder containing 80% sucrose by weight. Purity checked by SDS PAGE. Useful as a chromatography size marker.
Store at 2-8°C. PROTECT FROM MOISTURE.