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Amino Acid Oxidase, L- - Assay

Source:
Crotalus adamanteus Venom
CAS:
9000-89-9
EC:
1.4.3.2
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Amino Acid Oxidase, L- Assay

Method
The reaction velocity is determined in a peroxidase coupled system by measuring the increase in A436 resulting from the oxidation of L-leucine. One unit oxidizes one micromole of L-leucine per minute at 25°C and pH 7.6 under the specified conditions.
Reagents
  • 0.2 M Triethanolamine buffer pH 7.6 containing 0.1% L-leucine and 0.0065% o-dianisidine
  • 1.0% Peroxidase: Dissolve Worthington Peroxidase (HPOD) at 10 mg/ml in water.
Enzyme

Dilute enzyme in reagent grade water to 0.05-0.2 units per milliliter.

Procedure

Adjust spectrophotometer to 436 nm and 25°C.

Pipette into cuvettes 0.01 ml of 10 mg/ml peroxidase and 2.9 ml of 0.2 M triethanolamine-leucine-o-dianisidine mixture.

Incubate in spectrophotometer at 25°C for 4-5 minutes to achieve temperature equilibration and record blank rate, if any. Add 0.1 ml of appropriately diluted enzyme and record increase in absorbance at 436 nm for 4-5 minutes. Calculate ΔA436 from the initial linear portion of the slope. Subtract blank rate if present.

Calculation

Amino Acid Oxidase, L- Products

Description
Activity
Code
Cat. #
Size
Price
Description
Amino Acid Oxidase, L-
Source:
Crotalus adamanteus Venom
An aqueous solution with toluene added as a preservative.
Store at 2-8°C. DO NOT FREEZE.
…
Activity
≥4 units per mg protein
Code
LAO
LS002763
2 mg
$88.00
LS002764
5 mg
$178.00
LS002766
Bulk
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The minimum amount for bulk packaging/pricing for this product is 100 x 1 mg. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.