For our international customers, please be advised that orders cannot be placed through our website by customers in countries with International Distributor representation.
Chymotrypsinogen A - Assay
Source:
Bovine Pancreas
CAS:
9035-75-0
Chymotrypsinogen A Assay
Method
Chymotrypsinogen is assayed first for intrinsic activity, then activated and re-assayed. The activity will be determined in the chymotrypsin assay as described above.
Reagents
- 1 N H2SO4
- 0.5 M Sodium potassium phosphate buffer, pH 7.6
- Trypsin solution: Dissolve Worthington trypsin (Code: TRL), at a concentration of 1 mg/ml in 0.001 N HCl. Prepare immediately before use.
Procedure
Intrinsic chymotrypsin activity: Dissolve enzyme at a concentration of 10 mg/ml in 0.001 N HCl. Assay for chymotrypsin as described above. Calculate units/mg protein.
Chymotrypsinogen activation: Dissolve 100 mg enzyme in 5 ml reagent grade water. Adjust pH to 3.0 with 1 N H2SO4. Add 2.5 ml of 0.5 M phosphate buffer pH 7.6 and readjust pH to 7.6 if necessary. Add 1 ml (1 mg) trypsin and dilute to a final volume of 10 ml with reagent grade water. A 1:500 dilution will probably be required. Assay immediately using the chymotrypsin assay as described above. Repeat the assay at 10 minute intervals until the activity begins to decline. As activation continues, dilution may increase. Calculate units/mg chymotrypsin activity.
Chymotrypsinogen A Products
Description
Activity
Code
Cat. #
Size
Price
Description
Chymotrypsinogen A, Purified
Source:
Bovine Pancreas
Five times crystallized, electrophoretically homogeneous. Supplied as a dialyzed, lyophilized powder.
Intrinsic activity <0.55% chymotrypsin.
Intrinsic activity <0.55% chymotrypsin.
Store at 2-8°C.
Code
CGC
Product details
LS005630
1 gm
$74.00
LS005623
5 gm
$260.00
LS005622
Bulk
---