Lactoperoxidase

Source:
Bovine Milk
CAS:
9003-99-0
EC:
1.11.1.7

I.U.B.: 1.11.1.7

Lactoperoxidase (LPO) is a glycoprotein with a hemin prosthetic group which may occur as a mixture of two isozymes. It has a molecular weight of 77,500 daltons. LPO catalyzes the hydrogen peroxide oxidation of iodide according to the following reaction:

2I- + H2O2 + 2H+ → I2 + 2H2O.

Iodide reacts directly with the heme group; upon addition of H2O2 the complex iodinates the substrate. LPO is inhibited by hydrazines. The assay procedure has been updated from that of Morrison to an ABTS®/H2O2 based method with increased sensitivity and reproducibility.

Unit Definition
One Unit reduces one micromole of hydrogen peroxide per minute at 25°C, pH 6.0. Note: This unit definition is based upon a new assay method. The previous method yielded approximately 2.8 times higher results.

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