Pectinase

Source:
Aspergillus niger
CAS:
9033-35-6
EC:
4.2.2.10

I.U.B.: 4.2.2.10

Purified pectinase is a multi-component preparation highly effective in depolymerizing plant pectins with varying degrees of esterification. The product contains substantial hemicellulase, cellulase, pectinesterase and xylanase activities which together with pectin lyase and polygalacturonase work synergistically to digest plant cell wall tissues. When used with Worthington purified cellulase, purified pectinase has been found to be highly successful for generating good yields of viable protoplasts in several plant systems, e.g., corn, soybean, red beet, sunflower, tomato and citrus. In general, a concentration range of 0.1% to 0.5% pectinase (with accompanying 0.5% to 1.5% cellulase) used at 24°C to 37°C for periods of 1 to 16 hours will yield good results.

Stability/Storage: Protect from moisture. If not using entire bottle at once, weigh into single-use aliquots on arrival and store tightly covered and dessicated, at 2-8°C. Material is very hygroscopic and can become tacky and difficult to weigh if exposed to moisture.

Technical Note: Pectinase is extremely hydroscopic; store dessicated to protect from moisture.

Related Products: Cellulase (CEL/CELF)

Unit Definition
One Unit releases 1µmole of D-galacturonic acid from polygalacturonic acid per minute at 37°C, pH 5.0.

Pectinase Products

Description
Activity
Code
Cat. #
Price
Size
Description
Pectinase
Source:
Aspergillus niger
A chromatographically purified preparation also containing hemicellulase, cellulase, pectin-esterase and xylanase activities. Suitable for plant protoplast isolation applications. Supplied as a dialyzed, lyophilized powder.
Store at 2-8°C. PROTECT FROM MOISTURE.
Activity
≥20 units per mg dry weight
Code
PASE
Cat. #
LS004297