Enzyme Manual
The Worthington Enzyme Manual contains technical information on enzymes including molecular weight, composition, activators, specificity, inhibitors, stability, assay method, optimum pH, ionic effects, temperature effects, extinction coefficient, and isoelectric point, as well as applications and extensive references.
Worthington Biochemical Corporation offers a complete method development kit containing an assortment of enzymes most frequently used in tissue dissociation and cell isolation procedures. The "Cell…
Cellulase refers to a group of enzymes which, acting together, hydrolyze cellulose.
Cellulose is a linear polysaccharide of glucose residues connected by β-1,4 linkages. Like chitin it is not…
Cholesterol esterase catalyzes the hydrolysis of sterol esters into their component sterols and fatty acids. The enzyme is found primarily in the pancreas, but has been detected in other tissues as…
Acetylcholinesterase (AChE) catalyzes the hydrolysis of acylcholinesters with a relative specificity for acetylcholine:
The enzyme is bound to cellular membranes of excitable tissue (synaptic…
Acylcholine acylhydrolase
Butyryl cholinesterase, (ButChE), catalyzes the hydrolysis of a number of choline esters:
The enzyme is found in mammalian blood plasma, liver, pancreas, intestinal…
Chymotrypsin is a serine endopeptidase produced by the acinar cells of the pancreas. Chymotrypsin becomes activated after proteolysis of chymotrypsinogen by trypsin. While trypsin hydrolyzes at…
Chymotrypsin is a serine endopeptidase produced by the acinar cells of the pancreas. Chymotrypsin becomes activated after proteolysis of chymotrypsinogen by trypsin. While trypsin hydrolyzes at…
Cl. histolyticum produces two distinct types of collagenase: Collagenase 1 is very active on native collagen and synthetic substrates but with negligible activity on gelatin and azocoll, and…
Clostripain is a cysteine-activated protease found, along with collagenase and other proteases, in culture filtrates of Clostridium histolyticum. It is unique in its specificity for the…
Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its…
Collagenases are endopeptidases that digest native collagen in the triple helix region. Collagens are the major fibrous component of animal extracellular connective tissue. Bacterial collagenases…
First crystallized by Sumner and Howell (1936), Concanavalin A (ConA) has proven to be an interesting and useful lectin. Lectins are proteins that react with specific terminal sugar residues and are…
Creatine kinase catalyzes the following reaction:
Creatine kinase (CK) is widely distributed and seems to be primarily concerned with ATP regeneration. According to Watts (1973) CK may represent…
Cytochrome c oxidase is the terminal enzyme in the respiratory chain, catalyzes the four-electron reduction of molecular oxygen and couples this reaction to the generation of a proton gradient across…
Bovine pancreatic deoxyribonuclease is an endonuclease that preferentially splits phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5’-phosphate terminated polynucleotides with a…
Porcine DNase II (pDNase II) is an acid endonuclease widely found in animal cells, mostly associated with lysosomes. It hydrolyzes the phosphodiester bonds of DNA under acidic conditions without the…
Deoxyribonucleic acid (DNA) is a double stranded, helical nucleic acid molecule that consists of nucleotide monomers, each composed of a deoxyribose sugar, a phosphate group, and one of four…
Dextranases catalyze the endohydrolysis of 1,6-alpha-glucosidic linkages in dextran.
NADPH: (acceptor) oxidoreductase
The diaphorases are a ubiquitous class of flavin-bound enzymes that catalyze the reduction of various dyes which act as hydrogen acceptors from the reduced form of…
T4 DNA ligase is an ATP-dependent ligase that catalyzes a joining reaction between DNA molecules. By joining the 3’-hydroxy and 5’-phosphate termini to form a phosphodiester, DNA ligases are…
