Neonatal Cardiomyocyte Isolation System References

Kit for performing five separate tissue dissociations each containing up to twelve hearts. Contains single use vials of purified collagenase and trypsin, CMF-HBSS, Leibovitz L-15 media and Falcon cell strainers along with a detailed protocol. The kit is use-tested by Worthington to assure performance.

A component of the NCIS kit. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 5 ml of HBSS or equivalent yields a solution of 300 units/ml of purified collagenase. Suitable for cell isolation and culture applications.

A component of the NCIS kit. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 2 ml of HBSS yields a solution of 500µg/ml of trypsin, Code: TRLS. Suitable for cell isolation and culture applications.

A component of the NCIS kit. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 1 ml of HBSS or equivalent yields a solution of 2 mg/ml of trypsin inhibitor, Code: SIC. Suitable for cell isolation and culture applications.

Sterile calcium and magnesium free Hank's balanced salt solution (CMFHBSS), pH 7.4, as supplied in the NCIS kit; 1 x 500 ml.

Leibovitz L-15 media powder, a component of the NCIS kit. Reconstitute entire contents of pouch, QS to 1 liter with cell culture grade water, and 0.22 micron filter. Suitable for cell isolation and culture applications.

Cell strainers (Falcon), components of the NCIS kit. Suitable for removal of tissue debris in cell isolation applications.