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Papain

Source:
Carica papaya Latex
CAS:
9001-73-4
EC:
3.4.22.2
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I.U.B.: 3.4.22.2

Papain is a sulfhydryl protease from Carica papaya latex. It has a molecular weight of 23 kDa and an optimum pH range of 6.0-7.0.  The action of papain on leucine methyl ester produces an insoluble polyleucine peptide. Papain breaks down the intercellular matrix of cartilage. Papain is activated by cysteine, sulfide, and sulfite. Stabilizing agents are EDTA, cysteine and dimercaptoethanol.

Stability/Storage: Stable for ~ 6months at 2-8°C.  Do not freeze aqueous suspensions.

Technical Notes: Papain preparations should be incubated in the activation solution before use to ensure full activity. Applications include antibody fragmentation and primary/neural cell isolation.

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Neutral Protease (Dispase, NPRO)
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Cell Isolation Optimizimg System (CIT)
Hepatocyte Isolation System (HIS)
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Papain Dissociiation System (PDS/PDS2)

Unit Definition

One Unit hydrolyzes one micromole of benzoyl-L-arginine ethyl ester per minute at 25°C, pH 6.2, after activation in a solution containing 1.1 mM EDTA, 0.067 mM mercaptoethanol and 5.5 mM cysteine-HCl for 30 minutes.

Papain Products

Description
Activity
Code
Cat. #
Size
Price
Description
Papain, Suspension
Source:
Carica papaya Latex
Supplied as a two times crystalline suspension in 50mM sodium acetate, pH 4.5. To ensure full activity, the enzyme should be incubated in a solution containing 1.1mM EDTA, 0.067mM mercaptoethanol and 5.5mM cysteine-HCl for 30 minutes. It is recommended that the enzyme be 0.22 micron filtered after dissolution and prior to use.
Store at 2-8°C.
…
Ice Pack required
Activity
Activates to at least 20 units per mg protein
Code
PAP
LS003124
25 mg
$40.00
LS003126
100 mg
$88.00
LS003127
1 gm
$595.00
LS003128
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 20000 x 1 mg. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
Description
Papain, Lyophilized
Source:
Carica papaya Latex
Supplied as a lyophilized powder prepared from a two times crystalline suspension, Code: PAP. To ensure full activity, the enzyme should be incubated in a solution containing 1.1mM EDTA, 0.067mM mercaptoethanol and 5.5mM cysteine-HCl for 30 minutes. It is recommended that the enzyme be 0.22 micron filtered after dissolution and prior to use.
Store at 2-8°C.
…
Activity
Activates to at least 15 units per mg protein
Code
PAPL
LS003118
25 mg
$44.00
LS003119
100 mg
$100.00
LS003120
1 gm
$685.00
LS003122
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 20000 x 1 mg. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
Description
PDS Kit, Papain Vial
Source:
Papaya Latex
A component of the Papain Dissociation System, for use in the tissue dissociation method of Huettner, J.E., and Baughman, R.W.: J. Neuroscience, 6, 3044 (1986). Contains papain, L-cysteine, and EDTA. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 5 ml of EBSS or equivalent yields a solution at 20 units of papain per ml in 1mM L-cysteine with 0.5mM EDTA.
Store at 2-8°C.
…
Activity
≥100 units per vial
Code
PAP2
LK003176
1 vi
$29.00
LK003178
5 vi
$98.00
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Papain Dissociation System
Cell Isolation Optimizing System
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Hepatocyte Isolation System
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Neonatal Cardiomyocyte Isolation System
Trypsin
Trypsin Inhibitors
Endoproteinase Lys-C