Neutral Protease (Dispase)

Source:
Bacillus polymyxa
CAS:
9001-92-7
EC:
3.4.24.28

I.U.B.: 3.4.24.28

Neutral protease (Dispase®) is a non-mammalian animal origin free (AOF) metallo, neutral protease, purified by methods developed at Worthington. Its mild proteolytic action makes the enzyme especially suitable for the preparation of primary cells and secondary (subcultivation) cell culture, since it is gentle on cell membranes. This protease is also used as a secondary enzyme in cell isolation and tissue dissociation applications, commonly used with collagense.

Characteristics of Neutral Protease (Dispase®) from Bacillus polymyxa

Molecular Weight: 36 kda.

pH Optimum: Stable over a wide pH range: 4.0-9.0, optimum pH 5.9-7.0.

Stability/Storage: Stable at 2-8°C for 12 months. Aliquot and store at -20°C after reconstitution with water or commonly used balanced salt solutions or media.

Specificity: Non-specific cleavage of peptide bonds containing leucine and phenylalanine.

Activators: Divalent cations including Ca2+, Mg2+, Mn2+, and Fe2+

Inhibitors: EDTA (1mM), EGTA, 1-10-phenanthroline and heavy metals

Application(s): Commonly used to separate skin epidermis from dermis leaving intact epithelial sheets and stem cell, hepatocyte and other cell isolation applications. However, due to the diversity of the variables involved, exact isolation conditions should be determined empirically for each cell/tissue application.

Related Products:

Collagenase (CLS1-4/CLSS1-4/CLSPA)
Deoxyribonuclease I (DP/D/DCLS/D2/DPFF/DPRF)
Elastase (ES/ESL/ESFF)
Hyaluronidase (HSE/HSEP)
Papain (PAP/PAPL/PAP2)
Proteinase K (PROK)
Trypsin (TL/TRL/TRL3/TRLS/TRTPCK)
Trypsin Inhibitors (LBI/OI/SI/SIC)

Cell Isolation Optimizing System (CIT)
Hepatocyte Isolation System (HIS)
Neonatal Cardiomyocyte Isolation System (NCIS)
Papain Dissociiation System (PDS/PDS2)

Unit Definition
One Unit releases one micromole of Folin positive amino acids, measured as tyrosine, at 37°C, pH 7.5, using casein as the substrate.

Neutral Protease (Dispase) Products

Description
Activity
Code
Cat. #
Price
Size
Description
Neutral Protease (Dispase), Purified, Animal Free/AF
Source:
Bacillus polymyxa
Animal Free/AF. Chromatographically purified. A lyophilized powder.
Store at 2-8°C.
Activity
≥4 units per mg dry weight
Code
NPRO
Cat. #
LS02100
Description
Neutral Protease, Partially Purified, Animal Free/AF
Source:
Bacillus polymyxa
Animal Free/AF. Partially purified. A lyophilized powder.
Store at 2-8°C.
Activity
≥0.1 units per mg dry weight
Code
NPRO2
Cat. #
LS02110