Trypsin

Source:
Bovine Pancreas
CAS:
9002-07-7
EC:
3.4.21.4

I.U.B.: 3.4.21.4

Trypsin is a pancreatic serine protease with substrate specificity based upon positively charged lysine and arginine side chains. It is derived from a 34 kDa inactive precursor zymogen, trypsinogen, after enzymatic removal of an N-terminal 6-amino acid leader sequence resulting in the 23.8 kDa trypsin molecule. The optimum pH is 8.0. Trypsin is inhibited by organophosphorus compounds such as diisopropylfluorophosphate and natural inhibitors from pancreas. Soybean, lima bean, and egg white are also sources of natural inhibitors. Trypsin cleaves amide and ester bonds of Arg and Lys. The Worthington Sequencing Grade Trypsin has been further purified to remove trace contaminating proteases and autolysis products which could interfere in trypsin digestion experiments, and exhibits a single band on PAGE.

Uses: For tissue culture work, Worthington trypsin, Codes: TRL, TRLS, TRLVMF and TRTVMF have been used by many researchers.

Product Codes: TRSEQZ, TRSEQII and TRTPCK are typically used for protein sequencing, mapping and structure studies.

Worthington modified sequencing grade trypsin, SequENZ™, Product Code: TRSEQZ, is subjected to extensive purification to remove contaminating proteases and tryptic autolysis by-products which could affect the specificity of the digestion process.  Subsequently, the enzyme is chemically modified to minimize the autolysis process as well as increase the stability. The modified trypsin is processed further to remove residual autodegradation products. The specificity of the enzyme is routinely checked after the chemical modification.

Stability/Storage: Most grades of Worthington trypsin are stable for 2-3 years when stored at 2-8°C. Protect from moisture.

Unit Definitions:

Note: 1mg trypsin ≥180 TAME units, 10,350 BAEE units, 3,450 USP/NF units.

Technical Notes: The Virus and Mycoplasma Free trypsin (Code: TRTVMF) has been filtered through an 0.22 micron pore size membrane, lyophilized, subjected to gamma irradiation, and tested for virus and mycoplasma.

Related Products:

  • Chymotrypsin (CDAG/CDS/CDI)
  • Collagenase (CLS1-4/CLSPA)
  • Deoxyribonuclease I (DP/D/DCLS/D2/DPFF/DPRF)
  • Hyaluronidase (HSE/HSEP)
  • Neutral Protease (Dispase, NPRO)
  • Proteinase K (PROK)
  • Trypsin Inhibitors (LBI/OI/SI/SIC)
  • Cell Isolation Optimizimg System (CIT)
  • Hepatocyte Isolation System (HIS)
  • Neonatal Cardiomyocyte Isolation System (NCIS)
  • Papain Dissociation System (PDS/PDS2)
Unit Definition
TAME Unit: One Unit hydrolyzes 1 µmole of p-toluene-sulfonyl-L-arginine methyl ester (TAME) per minute at 25°C, pH 8.2, in the presence of 10 mM calcium.  

Trypsin Products

Description
Activity
Code
Cat. #
Price
Size
Description
SequENZ® Trypsin, Modified, Sequencing Grade
Source:
Bovine Pancreas
Trypsin, treated with L-(tosylamido-2-phenyl) ethyl chloromethyl ketone to inhibit contaminating chymotryptic activity, chemically modified to promote stability and further purified to remove autolysis fragments, resulting in a highly stable trypsin product resistant to autolysis while retaining specificity.
Store at -20°C. PROTECT FROM MOISTURE.
Ice Pack required
Activity
≥150 units per mg protein (at least 8,625 BAEE/2875 USP/NF units per mg protein)
Code
TRSEQZ
Cat. #
LS02120
Description
SequENZ® Trypsin, Modified, Sequencing Grade, Solution
Source:
Bovine Pancreas
Ready to use liquid preparation of Trypsin, treated with L-(tosylamido-2-phenyl) ethyl chloromethyl ketone to inhibit contaminating chymotryptic activity, chemically modified to promote stability and further purified to remove autolysis fragments, resulting in a highly stable trypsin product resistant to autolysis while retaining specificity. Supplied as 1mgP/ml in 1.0mM HCl.
Store at 2-8°C.
Ice Pack required
Code
TRSEQZS
Cat. #
LS02150
Description
Trypsin, Purified, Sequencing Grade II
Source:
Bovine Pancreas
Bovine trypsin that has been treated with L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK) to inhibit contaminating chymotryptic activity and extensively purified to remove autolysis products. Supplied as a lyophilized powder.
Store at -20°C. PROTECT FROM MOISTURE.
Ice Pack required
Activity
≥150 units per mg protein (at least 8,625 BAEE/2875 USP/NF units per mg protein)
Code
TRSEQII
Cat. #
LS02115
Description
Trypsin, TPCK Treated
Source:
Bovine Pancreas
A chromatographically purified, diafiltered, lyophilized powder that has been treated with L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK) to inhibit contaminating chymotryptic activity [Kostka, V., and Carpenter, F.: JBC, 239, 1799 (1964)].
Store at 2-8°C. PROTECT FROM MOISTURE.
Activity
≥180 units per mg protein (10,350 BAEE/3,450 USP/NF units per mg protein)
Code
TRTPCK
Cat. #
LS003740
Description
Trypsin
Source:
Bovine Pancreas
Supplied as a chromatographically purified, diafiltered and lyophilized powder.
Store at 2-8°C. PROTECT FROM MOISTURE.
Activity
≥180 units per mg protein
Code
TRL3
Cat. #
LS003708
Description
Trypsin
Source:
Bovine Pancreas
Supplied as a dialyzed and lyophilized powder.
Store at 2-8°C. PROTECT FROM MOISTURE.
Activity
≥180 units per mg protein
Code
TRL
Cat. #
LS003702
Description
Trypsin, Partially Purified
Source:
Bovine Pancreas
Trypsin, partially purified, dialyzed and lyophilized powder.
Store at -20°C. PROTECT FROM MOISTURE.
Activity
≥150 units per mg protein
Code
TL
Cat. #
LS003665
Description
Trypsin, TPCK-Treated, Irradiated
Source:
Bovine Pancreas
Chromatographically purified trypsin treated with L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK) to inhibit contaminating chymotryptic activity according to Kostka, V., and Carpenter, F.H.: JBC, 239, 1799 (1964), Code: TRTPCK, lyophilized, irradiated and tested for the absence of mycoplasma and extraneous virus according to 9 CFR 113.53c. Each vial is filled to contain 100 mg.
Store at 2-8°C.
Activity
≥180 units per mg protein
Code
TRTVMF
Cat. #
LS003750
Description
Trypsin, 2X, Sterile, Irradiated
Source:
Bovine Pancreas
Purified trypsin (Code: TRL), lyophilized, irradiated and tested for the absence of mycoplasma and extraneous virus according to 9 CFR113.53c. Each vial is filled to contain 100 mg.
Store at 2-8°C.
Activity
≥180 units per mg protein (10,350 BAEE/3,450 USP/NF units per mg protein)
Code
TRLVMF
Cat. #
LS004454
Description
Trypsin, 0.22µ Filtered
Source:
Bovine Pancreas
Trypsin chromatographically purified, diafiltered (Code TRL3) filtered thorugh a 0.22 micron pore size membrane and lyophilized in sterile vials. This product is not tested for pyrogenicity.
Store at 2-8°C.
Activity
≥180 units per mg protein (10,350 BAEE/3,450 USP/NF units per mg protein)
Code
TRLS
Cat. #
LS003736