Proteinase K

Source:
Recombinant PROK produced in yeast
CAS:
39450-01-6
EC:
3.4.21.64

Native Proteinase K, product codes PROK and PROKS have been discontinued and superseded with Recombinant Proteinase K, product codes PROKR and PROKRS. The recombinant products meet the same specifications as the native products.

Proteinase K is a serine protease with broad specificity towards aliphatic, aromatic and other hydrophobic amino acids. PROK has a molecular weight of 27,000 daltons and is Ca2+ dependent. It is not inactivated by metal ion chelating agents such as EDTA, sulfhydryl reagents, PCMB, TLCK, or TPCK. It also retains activity in 0.5% SDS. It can be inhibited by PMSF or DFP.

Stability/Storage: The lyophilized powder is stable for one year at 2-8°C. Solutions in 50mM Tris-HCl, pH 8.0 with 1mM CaCl2 are stable for months at 2-8°C. Store at 2-8°C.

Related Products:

Albumin, Nuclease-Free (BSANF)
Deoxyribonuclease I (DP/D/DCLS/D2/DPFF/DPRF)
E•RASE RNase A/T1 Blend (RCT)
Histones (H, NHL)
Lysozyme (LY/LYSF)
Nuclease, Micrococcal (NFCP)
Nuclease, S1 (SINUC/SINUCL)
Nucleic Acids, DNA, E.coli, Lambda/Fragments,RNA
Phosphatase, Alkaline (CAP/BAPF/BAPC/BAPSF/PC)
Phosphodiesterase I (VPH)
Phosphodiesterase II (SPH)
Reverse Transcriptase, Recombinant HIV (RTHIV)
Ribonuclease A (R/RAF/RASE/RS/RPDF)
Ribonuclease T1, Animal Origin Free (RT1S)

Unit Definition

One Unit releases one micromole of Folin positive amino acids, measured as tyrosine, at 37°C, pH 7.5, using urea denatured hemoglobin as the substrate.

Proteinase K Products

Description
Activity
Code
Cat. #
Price
Size
Description
Proteinase K, Solution
Source:
Tritirachium album limber
A concentrated, ready to use liquid formulation. Proteinase K prepared at 20mg/ml in 10mM Tris-HCl, 1mM calcium acetate, pH 7.5 containing 50% glycerol. DNase- and RNase- free.
Store at -20°C.
Ice Pack required
Activity
≥400 u/ml
Code
PROKS
Cat. #
Description
Proteinase K (Recombinant), Animal Free/AF
Source:
Recombinant PROK produced in yeast
Animal Free/AF, Molecular Biology Grade purified recombinant Tritiachium album Proteinase K produced in yeast. Free of DNase and RNase. Supplied as a lyophilized powder. Store at 2-8°C.
Store at 2-8°C.
Activity
>20 units per mg dry weight
Code
PROKR
Cat. #
LS004248
Description
Proteinase K, (Recombinant) Solution
Source:
Tritirachium album limber
A concentrated, ready to use liquid formulation. Recombinant Proteinase K prepared at 20mg/ml in  10mM Tris-HCl, 1mM calcium acetate, pH 7.5 containing 50% glycerol. DNase- and RNase- free. Store at -20°C
Store at -20°C.
Ice Pack required
Activity
>400 u/ml
Code
PROKRS
Cat. #
LS004254