Papain Dissociation System References

Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's balanced salt solution (EBSS), and an inhibitor vial for use in the tissue dissociation method of Huettner, J.E., and Baughman, R.W.: J. Neuroscience, 6, 3044 (1986). Use tested by Worthington using new-born rat pup spinal cord. The package contains sufficient materials for dissociation of five separate tissue aliquots of up to 0.3-0.4 cm3 each.

Complete kit as described for product Code: PDS, but without the Earle's Balanced Salt Solution (EBSS).

A component of the Papain Dissociation System, for use in the tissue dissociation method of Huettner, J.E., and Baughman, R.W.: J. Neuroscience, 6, 3044 (1986). Contains papain, L-cysteine, and EDTA. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 5 ml of EBSS or equivalent yields a solution at 20 units of papain per ml in 1mM L-cysteine with 0.5mM EDTA.

A component of the Papain Dissociation System. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 0.5 ml of EBSS or equivalent yields a solution of 2000 units/ml of deoxyribonuclease (1 mg/ml).

Ovomucoid protease inhibitor and bovine serum albumin which is 0.22 micron filtered and lyophilized in autoclaved vials to contain 10 mg/ml each upon reconstitution with 32 ml of EBSS.

Earle's balanced salt solution (EBSS) as supplied in the Papain Dissociation System.