Pepsin

Source:
Porcine Stomach
CAS:
9001-75-6
EC:
3.4.23.1

I.U.B.: 3.4.23.1

Pepsin is an acidic protease. Its inactive zymogen precursor, pepsinogen, is produced in the stomach mucosa. There are several pepsins designated A, B, C, and D. Pepsin A, the major component, has a molecular weight of 35,000 daltons and an optimum pH of approximately 1.0 for substrates such as casein or hemoglobin if the substrate is native protein. Pepsin cleaves proteins preferentially at carboxylic groups of aromatic amino acids such as phenylalanine and tyrosine. It will not cleave at bonds containing valine, alanine or glycine. Pepsin is assayed based on the method of Anson J. Gen. Physiol., 22, 79 (1938) using hemoglobin as the substrate. Pepsin is unstable above pH 6.

Stability/Storage: Pepsin for 1-2 years at 2-8°C.

Related Products:

Collagenase (CLS1-4/CLSPA)
Deoxyribonuclease I (DP/D/DCLS/D2/DPFF/DPRF)
Hemoglobin (HB)
Hyaluronidase (HSE/HSEP)
Neutral Protease (Dispase, NPRO)
Proteinase K (PROK)
Unit Definition

One Unit releases 0.001 A280 as TCA soluble hydrolysis products from denatured hemoglobin per minute at 37°C. One FIP Unit, expressed as micromoles of tyrosine equivalents liberated per minute at 25°C, can be calculated as follows: 1 Worthington Unit x 0.0071 = FIP units.

Pepsin Products

Description
Activity
Code
Cat. #
Size
Price
Description
Pepsin A
Source:
Porcine Stomach
Two times crystallized from dilute alcohol. A lyophilized powder.
Store at 2-8°C.
Activity
≥2,500 units per mg dry weight
Code
PM
LS003319
1 gm
$100.00
LS003317
10 gm
$700.00
LS003322
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 500000 x 1 gm. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
Description
Pepsin A, Purified
Source:
Porcine Stomach
Salt-precipitated protein. A dialyzed, lyophilized powder. 
Store at 2-8°C.
Activity
≥3,000 units per mg protein
Code
PM2
LS003324
1 gm
$70.00
LS003326
10 gm
$500.00
LS003328
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 200 x 1 gm. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.