Deoxyribonuclease I, Recombinant, AOF

Source:
Pichia pastoris
CAS:
9003-98-9
EC:
3.1.21.1

Bovine pancreatic deoxyribonuclease I produced recombinantly in yeast, Pichia pastoris, to decrease levels of contaminating RNase and eliminate potential pathogens associated with animal based materials.

Bovine pancreas is a rich source of RNase A which is often found in many commercial DNase preparations. Producing DNase I by recombinant means in an organism with much lower levels of endogenous RNase greatly facilitates purification of an enzyme with undetectable levels of RNase. The processes involved in the production and isolation of recombinant DNase I are completely devoid of animal based components which eliminates the possibility of introducing animal derived pathogens into bioprocessing procedures.

Recombinant DNase I is suitable for such applications as:

• Removing genomic DNA from RNA preparations prior to RT-PCR

• Degradation of DNA templates after transcription reactions

• Removing unwanted DNA from samples prior to Northern blotting

• Removing DNA during biopharma and bioprocessing procedures

Unit Definition:<

Note: Kunitz units as reported by other suppliers can be 2 to 4 times higher than Kunitz units as measured at Worthington. As measured at Worthington, One Kunitz unit digests 1mg of calf thymus (or pUC19 or l-phage) DNA in 10 minutes at 37oC in 50mM Tris, 1mM Mg2+, 1mM Ca2+, pH 7.8. Correlation of digestion units with Kunitz units may be different in other buffer systems.

Storage Buffer (DR1S): 5mM calcium acetate, 4mg/ml glycine, pH 5.0 and 50% glycerol.

DNase I Reaction Buffer (10X): 500mM Tris-HCl, 10mM MgSO4, 1mM CaCl2, pH 7.8, provided.

Related Products:

Albumin, Nuclease-Free (BSANF)
Deoxyribonuclease II (HDA/HDAC/HDACS)
E•RASE RNase A/T1 Blend (RCT)
Histones (H, NHL)
Lysozyme (LY/LYSF) 
Nuclease, Micrococcal (NFCP)
Nuclease, S1 (SINUC/SINUCL)
Nucleic Acids, DNA, E.coli, Lambda/Fragments,RNA
Phosphatase, Alkaline (CAP/BAPF/BAPC/BAPSF/PC)
Phosphodiesterase I (VPH) 
Phosphodiesterase II (SPH) 
Proteinase K (PROK/PROKS)
Reverse Transcriptase, Recombinant HIV (RTHIV) 
Ribonuclease A (R/RAF/RASE/RS/RPDF)
Ribonuclease T1, Animal Origin Free (RT1S)

 

Unit Definition

/span> One Unit causes an increase in absorbance at 260nm of 0.001 per minute at 25oC when acting upon highly polymerized DNA at pH 5.0, which is the same as other Worthington DNase I products.

Deoxyribonuclease I, Recombinant, AOF Products

Description
Activity
Code
Cat. #
Price
Size
Description
Deoxyribonuclease I, Recombinant, Protease and RNase Free, Animal Free/AF
Source:
Pichia pastoris
Recombinant Bovine pancreatic Deoxyribonuclease 1 produced in Pichia pastoris. Chromatographically purified. Free of animal derived components, RNases, and proteases. A lyophilized powder containing glycine as a stabilizer.
Store at 2-8°C. PROTECT FROM MOISTURE.
Activity
≥5000 Kunitz units per mg protein
Code
DR1
Cat. #
LS006361
Description
Deoxyribonuclease I, Recombinant, Solution, Animal Free/AF
Source:
Pichia pastoris
Animal Free/AF. Recombinant Bovine pancreatic deoxyribonuclease 1 produced in Pichia pastoris. Chromatographically purified. Free of animal derived components, RNases & proteases. A liquid preparation in 5mM Calcium Acetate, 4mg/ml glycine, pH 5.0 and 50% glycerol. Supplied with 10x reaction buffer. Store at -20°C. 
Store at -20°C.
Ice Pack required
Activity
≥2 Kunitz units per microliter
Code
DR1S
Cat. #
LS006353
Description
Deoxyribonuclease I, Recombinant, Animal Free/AF Bioprocess Grade
Source:
Pichia pastoris
DNase I, Recombinant, Produced in Pichia pastoris, Animal Free, Bioprocess Grade
 Chromatographically purified bioprocessing grade. Supplied as > 2,000 units per lyophilized powder containing glycine mg dry weight as a stabilizer. For the removal of DNA in bioprocessing and primary stem cell isolation application. May contain protease and RNase. Store at 2-8°C. PROTECT FROM MOISTURE.
Store at 2-8°C.
Activity
≥2,000 Kunitz units per mg dry weight
Code
DR2
Cat. #
LS006320