Phosphodiesterase I

Source:
Crotalus adamanteus Venom
CAS:
9025-82-5
EC:
3.1.4.1

I.U.B.: 3.1.4.1

Venom exonuclease (Phosphodiesterase I) successively hydrolyzes 5'-mononucleotides from 3'-OH-terminated ribo- and deoxyribo-oligonucleotides. The enzyme has an optimal pH range of 9.8-10.4 and a molecular weight of 115 kDa. Phosphodiesterase is inhibited by reducing agents such as glutathione, cysteine and ascorbic acids. It is completely inhibited by 5mM EDTA while ATP, ADP and AMP are partial inhibitors. The enzyme has an absolute requirement for Mg2+.

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Unit Definition
One Unit hydrolyzes one micromole of p-nitrophenyl thymidine-5-phosphate per minute at 25°C, pH 8.9.

Phosphodiesterase I Products

Description
Activity
Code
Cat. #
Price
Size
Description
Phosphodiesterase I
Source:
Crotalus adamanteus Venom
Purified by the method of Williams, Sung and Laskowski, JBC, 236, 1130 (1961). Further treated to inactivate contaminating 5'-nucleotidase activity according to Sulkowski and Laskowski: BBA, 240, 443 (1961). Lyophilized in vials.
Store at -20°C.
Ice Pack required
Activity
≥20 units per mg dry weight
Code
VPH
Cat. #
LS003926