Phosphatase, Alkaline

CAS:
9001-78-9
EC:
3.1.3.1

Alkaline phosphatase is a broad term associated with non-specific phosphomonoesterases with an alkaline pH optimum.

Source/Unit Definitons:
CAP: Calf intestine.  One Worthington Unit hydrolyzes 1µmole of p-nitrophenol phosphate per minute at 37°C, pH 9.8.

BAPF, BAPC, BAPSF: E. coli. One Unit hydrolyzes 1µmole of p-nitrophenol phosphate per minute at 25°C, pH 8.0.

PC: Chicken Intestine.  One Unit hydrolyzes 1µmole of o-carboxyphenol phosphate per minute at 25°C, pH 8.8.

Technical Notes: Worthington chicken intestine alkaline phosphatase (Code: PC) is the preparation used in the NF/USP dexamethasone phosphate measurement.

Related Products:

Albumin, Nuclease-Free (BSANF)
Deoxyribonuclease I (DP/D/DCLS/D2/DPFF/DPRF)
Histones (H, NHL)
Lysozyme (LY/LYSF)
Nuclease, Micrococcal (NFCP)
Nuclease, S1 (SINUC/SINUCL)
Nucleic Acids, DNA, E.coli, Lambda/Fragments,RNA
Phosphodiesterase I (VPH)
Phosphodiesterase II (SPH)
Proteinase K (PROK/PROKS)
Reverse Transcriptase, Recombinant HIV (RTHIV)
Ribonuclease A (R/RAF/RASE/RS/RPDF)
Ribonuclease T1, Animal Origin Free (RT1S)

Phosphatase, Alkaline Products

Description
Activity
Code
Cat. #
Size
Price
Description
Phosphatase, Alkaline, Purified
Source:
Calf Intestine
Chromatographically purified, EIA grade. A solution in 50% glycerol containing 5mM Tris, 5mM MgCl2 and 0.1mMZnCl2. Protein concentration is approximately 20mg/ml.
Store at 2-8°C.
Activity
≥3,000 units per mg protein (37°C, pH9.8, DEA)
Code
CAP
LS004228
1 mg
$120.00
LS004230
5 mg
$525.00
LS004234
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 400 x 1 mg. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
Description
Phosphatase, Alkaline, Purified
Source:
E. coli
Chromatographically purified from Code: BAPC. Ribonuclease <0.002% by weight as RNase A using a poly C assay. Phosphodiesterase not detectable when assayed at 0.1 mg/ml with p-nitrophenyl thymidine 5' phosphate. A suspension in 2.6M ammonium sulfate, pH 8.0.
Store at 2-8°C.
Activity
≥30 units per mg protein (25°C, pH 8.0)
Code
BAPF
LS006130
1 mg
$41.00
LS006124
5 mg
$149.00
LS006123
25 mg
$650.00
LS006122
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 500 x 1 mg. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
Description
Phosphatase, Alkaline
Source:
E. coli
Chromatographically purified. A suspension in 2.6M ammonium sulfate, pH 8.0.
Store at 2-8°C.
Activity
≥20 units per mg protein (25°C, pH 8.0)
Code
BAPC
LS005129
5 mg
$87.00
LS005130
10 mg
$141.00
LS005131
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 200 x 1 mg. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
Description
Phosphatase, Alkaline
Source:
E. coli
Partially purified. A suspension in 2.6M ammonium sulfate, pH 8.0.
Store at 2-8°C.
Activity
≥10 units per mg protein (25 degrees C, pH 8.0)
Code
BAPSF
LS004081
10 mg
$60.00
LS004082
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 200 x 1 mg. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
Description
Phosphatase, Alkaline
Source:
Chicken Intestine
Partially purified. A dried powder. Used in the NF/USP dexamethasone phosphate assay.
Store at 2-8°C.
Activity
≥0.9 units per mg dry weight (25°C pH 8.8)
Code
PC
LS003172
250 mg
$130.00
LS003171
1 gm
$445.00
LS003170
5 gm
$1,900.00
LS003174
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 100000 x 1 mg. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.