Phosphatase, Alkaline

CAS:
9001-78-9
EC:
3.1.3.1

Alkaline phosphatase is a broad term associated with non-specific phosphomonoesterases with an alkaline pH optimum.

Source/Unit Definitons:
CAP:
Calf intestine.  One Worthington Unit hydrolyzes 1µmole of p-nitrophenol phosphate per minute at 37°C, pH 9.8.

BAPF, BAPC, BAPSF: E. coli. One Unit hydrolyzes 1µmole of p-nitrophenol phosphate per minute at 25°C, pH 8.0.

PC: Chicken Intestine.  One Unit hydrolyzes 1µmole of o-carboxyphenol phosphate per minute at 25°C, pH 8.8.

Technical Notes: Worthington chicken intestine alkaline phosphatase (Code: PC) is the preparation used in the NF/USP dexamethasone phosphate measurement.

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Phosphatase, Alkaline Products

Description
Activity
Code
Cat. #
Price
Size
Description
Phosphatase, Alkaline, Purified
Source:
Calf Intestine
Chromatographically purified, EIA grade. A solution in 50% glycerol containing 5mM Tris, 5mM MgCl2 and 0.1mMZnCl2. Protein concentration is approximately 20mg/ml.
Store at 2-8°C.
Activity
≥3,000 units per mg protein (37°C, pH9.8, DEA)
Code
CAP
Cat. #
LS004228
Description
Phosphatase, Alkaline, Purified
Source:
E. coli
Chromatographically purified from Code: BAPC. Ribonuclease <0.002% by weight as RNase A using a poly C assay. Phosphodiesterase not detectable when assayed at 0.1 mg/ml with p-nitrophenyl thymidine 5' phosphate. A suspension in 2.6M ammonium sulfate, pH 8.0.
Store at 2-8°C.
Activity
≥30 units per mg protein (25°C, pH 8.0)
Code
BAPF
Cat. #
LS006130
Description
Phosphatase, Alkaline
Source:
E. coli
Chromatographically purified. A suspension in 2.6M ammonium sulfate, pH 8.0.
Store at 2-8°C.
Activity
≥20 units per mg protein (25°C, pH 8.0)
Code
BAPC
Cat. #
LS005129
Description
Phosphatase, Alkaline
Source:
E. coli
Partially purified. A suspension in 2.6M ammonium sulfate, pH 8.0.
Store at 2-8°C.
Activity
≥10 units per mg protein (25 degrees C, pH 8.0)
Code
BAPSF
Cat. #
LS004081
Description
Phosphatase, Alkaline
Source:
Chicken Intestine
Partially purified. A dried powder. Used in the NF/USP dexamethasone phosphate assay.
Store at 2-8°C.
Activity
≥0.9 units per mg dry weight (25°C pH 8.8)
Code
PC
Cat. #
LS003172