Ribonuclease T2, Recombinant

Source:
Aspergillus oryzae
CAS:
37278-25-4
EC:
4.6.1.19

Aspergillus oryzae Ribonuclease T2 is a member of the RNase T2 family of endonucleases that are present in a wide variety of microbial, plant and animal species. In contrast to Aspergillus oryzae Ribonuclease T1, which is an exclusively guanylic-acid specific endonuclease, all RNase T2-like enzymes are essentially base non-specific. However, RNase T2 endonucleases from different species can show slight base preferences. The fungal enzymes, including Aspargillus oryzae RNaseT2, show slight base preference in the following order: A>G>C, U. RNase T2 cleaves between the 3’-phosphate residue of one base and the 5’-OH residue of the adjacent nucleotide forming a 2’, 3’-cyclic phosphate intermediate followed by the generation of oligonucleotides with 3’-phosphate residues. RNase T2 has a molecular weight of 36 kDa and 12-15% of its mass is composed of carbohydrate. It has an isoelectric point of 5.0 and optimum activity at pH 4.5. RNase T2 is strongly inhibited by Cu++, Zn++ and Hg++ and to a lesser degree by Ca++, Mg++and heparin. Mononucleotides and RNase T2 digestion products can also act as competitive inhibitors. EDTA will stimulate activity, especially in the presence of divalent cations.

Uses: Ribonuclease T2 is often used for 3’ analysis of RNA and RNase protection assays.

Animal Origin Free (AOF): This enzyme is also used as a non-mammalian source of RNase in various applications.

Stability/Storage: Stable at 12-18 months at 2-8°C. Store at 2-8°C.

Unit Definition:

Unit Definition

One unit will cause an increase in absorbance of 1.0 at 260 nm at 37°C, pH 4.5 in 15 minutes.

Ribonuclease T2, Recombinant Products

Description
Activity
Code
Cat. #
Price
Size
Description
Ribonuclease T2, Recombinant, Animal Free/AF
Source:
Aspergillus oryzae
Animal Free/AF. Recombinant aspergillus oryzae (non-mammalian) broad specificity Ribonuclease T2 highly purified & produced in Pichia pastoris prepared with non-animal components. Supplied as lyophilized powder.
Store at 2-8°C.
Activity
≥10,000 units per mg protein
Code
RT2R
Cat. #
LS01501