Nuclease, S1

Source:
Aspergillus oryzae
CAS:
37288-25-8
EC:
3.1.30.1

Nuclease S1 isolated from certain Neurospora and Aspergillus species specifically hydrolyzes both terminal and internal phosphodiester bonds of single-stranded DNA and RNA. Nuclease S1 has a molecular weight of approximately 34 kDa and exists as a monomer. The optimum pH range is 4.0-4.6, and it is activated by Zn2+ and/or Ca2+. Inhibitors are EDTA, citrate and high concentrations of SDS.

Stability/Storage: For long term storage in solution, for up to six months, dilute NUCSI to ≥6000 u/ml in water and freeze in aliquots. Dilute solutions can be stabilized by adding 0.1% albumin (Worthington Code: BSANF) and 10% glycerol.

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Unit Definition

One Unit hydrolyzes one microgram of denatured calf thymus DNA per minute at 37°C, pH 4.6.

Nuclease, S1 Products

Description
Activity
Code
Cat. #
Price
Size
Description
Nuclease, S1
Source:
Aspergillus oryzae
Chromatographically purified. Specific for single-stranded DNA (ssDNA) degradation. Activity on native (ds) DNA undetectable under the assay conditions. A frozen solution in 30mM sodium acetate, pH 4.6, 50mM NaCl, 1mM ZnCl2, and 50% glycerol.
Store at -20°C.
Ice Pack required
Activity
≥100,000 to 500,000 units per ml
Code
SINUC
Cat. #
LS04070