Nuclease S1 isolated from certain Neurospora and Aspergillus species specifically hydrolyzes both terminal and internal phosphodiester bonds of single-stranded DNA and RNA. Nuclease S1 has a molecular weight of approximately 34 kDa and exists as a monomer. The optimum pH range is 4.0-4.6, and it is activated by Zn2+ and/or Ca2+. Inhibitors are EDTA, citrate and high concentrations of SDS.
Stability/Storage: For long term storage in solution, for up to six months, dilute NUCSI to ≥6000 u/ml in water and freeze in aliquots. Dilute solutions can be stabilized by adding 0.1% albumin (Worthington Code: BSANF) and 10% glycerol.
Albumin, Nuclease-Free (BSANF)
Deoxyribonuclease I (DP/D/DCLS/D2/DPFF/DPRF)
Histones (H, NHL)
Nuclease, Micrococcal (NFCP)
Nucleic Acids, DNA, E.coli, Lambda/Fragments,RNA
Phosphatase, Alkaline (CAP/BAPF/BAPC/BAPSF/PC)
Phosphodiesterase I (VPH)
Phosphodiesterase II (SPH)
Proteinase K (PROK/PROKS)
Reverse Transcriptase, Recombinant HIV (RTHIV)
Ribonuclease A (R/RAF/RASE/RS/RPDF)
Ribonuclease T1, Animal Origin Free (RT1S)
One Unit hydrolyzes one microgram of denatured calf thymus DNA per minute at 37°C, pH 4.6.
Nuclease, S1 Products
Chromatographically purified. Specific for single-stranded DNA (ssDNA) degradation. Activity on native (ds) DNA undetectable under the assay conditions. A frozen solution in 30mM sodium acetate, pH 4.6, 50mM NaCl, 1mM ZnCl2, and 50% glycerol.
Store at -20°C.
Ice Pack required
≥100,000 to 500,000 units per ml