Deoxyribonucleic Acid and Related Products

CAS:
9007-49-2

Worthington offers DNA purified from these sources:

Calf thymus: (Code: DNA) Prepared and purified by a method developed at Worthington to have lower protein and RNA contamination than most other commercial preparations. This highly polymerized DNA is an excellent substrate for deoxyribonuclease. A sodium salt, it must be converted by adding magnesium ions to be susceptible to DNase.

Calf thymus DNA, covalently bound to cellulose is also available:
DNA Cellulose, Double-Stranded
(Code: DNACELDS)
DNA Cellulose, Single-Stranded (Code: DNACELSS)

Salmon Testes: (Code: SDNA) Prepared by a modification of the method of Emanuel and Chaikoff, J. Biol. Chem., 203, 164, (1953). A minimum of 75% native nucleic acid.

Salmon Testes DNA, Denatured & Fragmented (Code: SDNAD) is also available. Prepared by mechanical shearing and heat denaturation.

Escherichia coli: (Code: DNAEC) Isolated as described by Marmur, J. Mol. Biol., 3, 208, 1961.

Lambda phage DNA (Code: DNAL) is prepared from CsCl purified phage and is purified to an A260/A280 ≥1.8. Homogeneous in agarose gel electrophoresis. A solution in 10 mM Tris-HCl pH 8.0 with 1 mM EDTA.

DNA fragments prepared by restriction endonuclease digestion of purified lambda DNA are available (Codes: DNALBSTE; DNALECOR; DNALHIND). Supplied as solutions in 10 mM Tris-HCl pH 8.0 with 1 mM EDTA.

Technical Note: One A260 unit = 50 µg DNA.

Stability/Storage: DNAL: Storage buffer 10 mM Tris-HCl, pH 8.0 containing 1 mM EDTA. Store at -20°C.  Once thawed keep at 2-8°C.

Related Products:

  • Albumin, Nuclease-Free (BSANF)
  • Deoxyribonuclease I (DP/D/DCLS/D2/DPFF/DPRF)
  • Deoxyribonuclease II (HDA/HDAC/HDACS)
  • E•RASE RNase A/T1 Blend (RCT)
  • Histones (H, NHL)
  • Lysozyme (LY/LYSF) 
  • Nuclease, Micrococcal (NFCP)
  • Nuclease, S1 (SINUC/SINUCL)
  • Phosphatase, Alkaline (CAP/BAPF/BAPC/BAPSF/PC)
  • Phosphodiesterase I (VPH) 
  • Phosphodiesterase II (SPH) 
  • Proteinase K (PROK/PROKS)
  • Reverse Transcriptase, Recombinant HIV (RTHIV) 
  • Ribonuclease A (R/RAF/RASE/RS/RPDF)
  • Ribonuclease T1, Animal Origin Free (RT1S)

Deoxyribonucleic Acid and Related Products Products

Description
Activity
Code
Cat. #
Price
Size
Description
Deoxyribonucleic Acid, Calf Thymus
Source:
Calf Thymus
Highly polymerized; hyperchromicity 27%. A substrate for deoxyribonuclease assays. Prepared by a method developed at Worthington to remove contaminating RNA and protein. Supplied dried.
Store at 2-8°C.
Code
DNA
Cat. #
LS002105
Description
DNA Cellulose, Double-Stranded
Source:
Calf Thymus
Prepared by a method developed at Worthington in which native, double-stranded calf thymus DNA is covalently bound to cellulose. Suitable for the purification of many DNA binding proteins such as polymerases, transcription factors, and terminators, etc. Supplied as a dry powder. One gram of DNA-cellulose will swell to 3-4ml when fully hydrated.
Store at 2-8°C.
Activity
≥3 mg DNA per gm dry weight
Code
DNACELDS
Cat. #
LS01120
Description
DNA Cellulose, Single-Stranded
Source:
Calf Thymus
Prepared by a method developed at Worthington in which denatured, single-stranded calf thymus DNA is covalently bound to cellulose. Suitable for the purification of many proteins that are associated with nucleic acids such as DNA/RNA polymerases and endo- and exo-nucleases and reverse transcriptases. Supplied as a dry powder. One gram of DNA-cellulose will swell to 3-4ml when fully hydrated.
Store at 2-8°C.
Activity
≥3 mg DNA per gm dry weight
Code
DNACELSS
Cat. #
LS01130
Description
Deoxyribonucleic Acid, Salmon Testes
Source:
Salmon Testes
Prepared by a modification to the method of Emanuel, C.F., and Chaikoff, I.L., : JBC, 203, 164 (1953). 75% native nucleic acid. Supplied dried.
Store at 2-8°C.
Code
SDNA
Cat. #
LS003554
Description
Deoxyribonucleic Acid, Denatured, Fragmented
Source:
Salmon Testes
Prepared from purified salmon testes DNA (Code: SDNA) by mechanical shearing and heat denaturation to an average fragment size of 200-1000 base pairs. To reverse any renaturation occurring during storage this material should be briefly boiled and rapidly chilled before use. Recommended concentration for use is 100µg/ml. A solution at 5 mg/ml in 0.05M NaCl.
Store at -20°C.
Ice Pack required
Code
SDNAD
Cat. #
LS01440
Description
Deoxyribonucleic Acid, Lambda
Source:
E. coli
Purified to an A260/A280 1.8 from purified phage. Homogeneous by agarose gel electrophoresis. Generates the characteristic five and eight bands after digestion with EcoR I and Hind III respectively. A solution in 10mM Tris-HCl, pH 8.0, with 1mM EDTA.
Store at -20°C.
Dry Ice required
Code
DNAL
Cat. #
LS01203
Description
Deoxyribonucleic Acid, Lambda, Hind III Fragments
Source:
Lambda DNA
DNA fragments prepared by the digestion of purified lambda DNA with the restriction endonuclease Hind III. On agarose gel electrophoresis the mixture separates into eight individual bands having the following number of base pairs: 23130, 9416, 6557, 4361, 2322, 2027, 564, and 125. (Note: A higher sample load may be required to clearly see the 564 and 125 base pair bands.) A solution in 10mM Tris-HCl, pH 8.0, with 1mM EDTA.
Store at -20°C.
Dry Ice required
Code
DNALHIND
Cat. #
LS01303
Description
Deoxyribonucleic Acid, Lambda, EcoR I Fragments
Source:
Lambda DNA
DNA fragments prepared by the digestion of purified lambda DNA with the restriction endonuclease EcoR I. On agarose gel electrophoresis the mixture separates into five individual bands having the following number of base pairs: 21226, 7421, 5804, 4878, and 3530. A solution in 10mM Tris-HCl, pH 8.0, with 1mM EDTA.
Store at -20°C.
Dry Ice required
Code
DNALECOR
Cat. #
LS01293
Description
Deoxyribonucleic Acid, Lambda, BstE II Fragments
Source:
Lambda DNA
DNA fragments prepared by the digestion of lambda DNA with the restriction endonuclease BstE II. On agarose gel electrophoresis the mixture separates into 14 individual bands having the following number of base pairs: 8454, 7242, 6369, 5686, 4822, 4324, 3675, 2323, 1929, 1371, 1264, 702, 224 and 117. A solution in 10mM Tris-HCl, pH 8.0, with 1mM EDTA.
Store at -20°C.
Dry Ice required
Code
DNALBSTE
Cat. #
LS01430
Description
Deoxyribonucleic Acid, E. coli
Source:
E. coli
Supplied as a dried powder purified from E. coli Type B cells (ATCC#11303) as described by Marmur, J. Mol. Biol., 3, 208 (1961).
Store at 2-8°C.
Code
DNAEC
Cat. #
LS004449