Hepatocyte Isolation System References

Kit for performing five separate adult rat liver perfusions. Contains five single use CLSH enzyme vials, five single use DNase vials, 10X CMF-Hank's Balanced Salt Solution, L-15 Media Powder, 0.15M MOPS buffer,7.5% Sodium Bicarbonate Solution, and optimized protocol. The kit is use-tested by Worthington to assure performance.

Enzyme vial of HIS kit that contains 20,000 units of collagenase (Code: CLS-1) and 30 units of elastase (Code: ESL), filtered through 0.22 micron membrane and lyophilized.

A component of the Papain Dissociation System. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 0.5 ml of EBSS or equivalent yields a solution of 2000 units/ml of deoxyribonuclease (1 mg/ml).

Sterile calcium and magnesium free Hank's balanced salt solution, 10X CMF-HBSS concentrate, as supplied in the HIS kit; 1 x 500 ml

Leibovitz L-15 media powder, a component of the NCIS kit. Reconstitute entire contents of pouch, QS to 1 liter with cell culture grade water, and 0.22 micron filter. Suitable for cell isolation and culture applications.

0.15M MOPS, pH 7.5, 0.22u filtered. Buffer concentrate used to buffer the constituted Leibovitz L-15 media in Hepatocyte Isolation System.

7.5% Sodium bicarbonate concentrate (NaHCO3), used to buffer the diluted CMF-HBSS; 1 x 100 ml