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Deoxyribonuclease II

Source:
Porcine Spleen
CAS:
9025-64-3
EC:
3.1.22.1
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I.U.B.: 3.1.22.1

Deoxyribonuclease II from porcine spleen has a molecular weight of 38 kDa. The enzyme is a glycoprotein endonuclease with dimeric structure. Optimum pH range is 4.5-5.0 at ionic strength 0.15 M. Deoxyribonuclease II (Acid DNase) hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3'-phosphates. It also acts on p-nitrophenylphosphodiesters at pH 5.6-5.9. Bernardi (BBRC, 17, 573, 1971) describes a three stage degradation of native DNA by DNase II.

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Unit Definition

One Unit causes an increase in absorbance at 260 nm of 0.001 per minute at 25°C, pH 4.6 using highly polymerized DNA as substrate.

Deoxyribonuclease II Products

Description
Activity
Code
Cat. #
Size
Price
Description
Deoxyribonuclease II
Source:
Porcine Spleen
A dialyzed, lyophilized powder.
Store at 2-8°C.
…
Activity
≥800 units per mg dry weight
Code
HDA
LS002425
80 ku
$85.00
LS002427
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 1600 x 1 ku. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
Description
Deoxyribonuclease II, Purified
Source:
Porcine Spleen
Chromatographically purified in a modification of the procedure of Bernardi, et al., BBA, 129, 1 (1966). A dialyzed, lyophilized powder.
Store at -20°C.
…
Ice Pack required
Activity
≥12,000 units per mg protein
Code
HDAC
LS005410
20 ku
$250.00
LS005411
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 400000 x 1 un. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
Description
Deoxyribonuclease II, Purified, Solution
Source:
Porcine Spleen
Chromatographically prepared. A solution in 50% glycerol.
Store at -20°C.
…
Ice Pack required
Activity
≥12,000 units per mg protein
Code
HDACS
LS005416
2 ku
$60.00
LS005418
5 ku
$140.00
LS005420
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 100 x 1 ku. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
Related Products
Albumin, Nuclease-Free
Deoxyribonuclease I
Histones
Lysozyme
Nuclease, Micrococcal
Nuclease, S1
Deoxyribonucleic Acid and Related Products
Ribonucleic Acid
Phosphatase, Alkaline
Phosphodiesterase II
Proteinase K
Reverse Transcriptase, Recombinant HIV
Ribonuclease A
Ribonuclease T1