Deoxyribonuclease II

Source:
Porcine Spleen
CAS:
9025-64-3
EC:
3.1.22.1

I.U.B.: 3.1.22.1

Deoxyribonuclease II from porcine spleen has a molecular weight of 38 kDa. The enzyme is a glycoprotein endonuclease with dimeric structure. Optimum pH range is 4.5-5.0 at ionic strength 0.15 M. Deoxyribonuclease II (Acid DNase) hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3'-phosphates. It also acts on p-nitrophenylphosphodiesters at pH 5.6-5.9. Bernardi (BBRC, 17, 573, 1971) describes a three stage degradation of native DNA by DNase II.

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Unit Definition

One Unit causes an increase in absorbance at 260 nm of 0.001 per minute at 25°C, pH 4.6 using highly polymerized DNA as substrate.

Deoxyribonuclease II Products

Description
Activity
Code
Cat. #
Price
Size
Description
Deoxyribonuclease II, Purified
Source:
Porcine Spleen
Chromatographically purified in a modification of the procedure of Bernardi, et al., BBA, 129, 1 (1966). A dialyzed, lyophilized powder.
Store at -20°C.
Ice Pack required
Activity
≥12,000 units per mg protein
Code
HDAC
Cat. #
LS005410
Description
Deoxyribonuclease II, Purified, Solution
Source:
Porcine Spleen
Chromatographically prepared. A solution in 50% glycerol.
Store at -20°C.
Ice Pack required
Activity
≥12,000 units per mg protein
Code
HDACS
Cat. #
LS005416