Reverse Transcriptase, Recombinant HIV

Source:
Recombinant protein produced in E. coli
CAS:
9068-38-6
EC:
2.7.7.49

I.U.B.: 2.7.7.49

Chromatographically purified heterodimer composed of 66kDa and 51kDa subunits. Supplied as a solution in 10mM potassium phosphate, pH 7.4, 1mM DTT and 20% glycerol. Primarily for AIDS research purposes; this enzyme has less fidelity than the AMV enzyme in other applications such as the preparation of cDNA from mRNA for cloning purposes.

Note: The current assay uses a more efficient template/primer combination that results in an approximate two-fold increase in specific activity compared to our previous assay. As a result, Worthington's package sizes contain the same amount of protein with twice as much activity based upon the new assay.

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Unit Definition

One Unit incorporates 1 nmole of tritiated d-TMP into acid precipitable products using poly(A)/oligo(dT)12-18 as the template/primer in 20 minutes at 37°C, pH 8.3.

Reverse Transcriptase, Recombinant HIV Products

Description
Activity
Code
Cat. #
Size
Price
Description
Reverse Transcriptase, Recombinant, HIV
Source:
Recombinant protein produced in E. coli
Chromatographically purified dimeric form with M.W. of 66 kDa and 51 kDa. A solution in 10mM potassium phosphate, pH 7.4, 1mM DTT and 20% glycerol.
Store at -20°C.
Dry Ice required
Activity
≥5,000 units per mg protein
Code
RTHIV
LS05003
200 un
$95.00
LS05006
5x200 un
$375.00
LS05000
Bulk
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The minimum amount for bulk packaging/pricing for this product is 20000 x 1 un. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.