Nuclease, S1 - Manual

Source:
Aspergillus oryzae
CAS:
37288-25-8
EC:
3.1.30.1

Nuclease S1, isolated from certain Neurospora and Aspergillus species, specifically hydrolyzes both terminal and internal phosphodiester bonds of single-stranded DNA and RNA. It is used to eliminate non-annealed polynucleotide tails and hair-pin loops in DNA-RNA or DNA-DNA duplexes in hybridization studies and in genetic recombination experiments.

Characteristics of Nuclease, S1
Molecular Weight

Approximately 32,000 - 36,000 daltons, exists as a monomer. (Vogt, V. 1973).

Optimal pH

4.0 - 4.6. (Vogt 1973 and Ando 1966).

Activators

Zn++ and/or Ca++. (Vogt 1973 and Ando 1966).

Inhibitors

EDTA, citrate (Vogt 1973 and Ando 1966) and a high concentration of SDS.

Storage

Store at -20°C.

Nuclease, S1 Products

Description
Activity
Code
Cat. #
Size
Price
Description
Nuclease, S1
Source:
Aspergillus oryzae
Chromatographically purified. Specific for single-stranded DNA (ssDNA) degradation. Activity on native (ds) DNA undetectable under the assay conditions. A frozen solution in 30mM sodium acetate, pH 4.6, 50mM NaCl, 1mM ZnCl2, and 50% glycerol.
Store at -20°C.
Ice Pack required
Activity
≥100,000 to 500,000 units per ml
Code
SINUC
LS04070
10 ku
$56.00
LS04072
50 ku
$155.00
LS04073
Bulk
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The minimum amount for bulk packaging/pricing for this product is 1000 x 1 ku. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.