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NUCxyme™ References

Source:
Komagataella phaffii (previously P. pastoris)
CAS:
9025-65-4
EC:
3.1.30.2
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Ball, T. , Saurugger, P. and Benedik, M.
The extracellular nuclease gene of Serratia marcescens and its secretion from Escherichia coli. ,
Gene
 57 , 183-92 , 1987
Benedik, M. and Strych, U.
Serratia Marcescens and its Extracellular Nuclease. ,
FEMS Microbiol Lett
 165 , 1-13 , 1998
Chen, C. , Beck, B. , Krause, K. and Pettitt, B.
Solvent participation in Serratia marcescens endonuclease complexes. ,
Proteins
 62 , 982-95 , 2006
Chen, C. , Krause, K. and Pettitt, B.
Advantage of being a dimer for Serratia marcescens endonuclease. ,
J Phys Chem B
 113 , 511-21 , 2009
Franke, I. , Meiss, G. , Blecher, D. , Gimadutdinow, O. , Urbanke, C. and Pingoud, A.
Genetic engineering, production and characterisation of monomeric variants of the dimeric Serratia marcescens endonuclease. ,
FEBS
 425 , 517-22 , 1998
Friedhoff, P. , Gimadutdinow, O. , Rüter, T. , Wende, W. , Urbanke, C. , Thole, H. and Pingoud, A.
A procedure for renaturation and purification of the extracellular Serratia marcescens nuclease from genetically engineered Escherichia coli. ,
Protein Expr Purif
 5 , 37-43 , 1994
Meiss, G. , Gast, F. and Pingoud, A.
The DNA/RNA non-specific Serratia nuclease prefers double-stranded A-form nucleic acids as substrates. ,
J Mol Biol
 288 , 377-90 , 1999
Vafina, G. , Zainutdinova, E. , Bulatov, E. and Filimonova, M.
Endonuclease from Gram-Negative Bacteria Is as Effective as Pulmozyme in the Hydrolysis of DNA in Sputum. , Front Pharmacol Vol. 9 , 114 , 2018

NUCxyme™ Products

Description
Activity
Code
Cat. #
Size
Price
Description
NUCxyme™, DNA/RNA Nuclease, Recombinant, AF
Source:
Komagataella phaffii (previously P. pastoris)
Recombinant Serratia marcescens nuclease produced in Komagataella phaffi. Chromatographically purified. Free of animal derived components and proteases.  A liquid preparation in 20mM Tris-HCl, 2mM MgCl2, 20mM NaCl, pH 8.0 and 50% glycerol.
Store at -20°C.
…
Ice Pack required
Activity
≥250,000 u/ml
Code
NUCAR
LS01522
25 ku
$125.00
LS01524
100 ku
$350.00
LS01525
Bulk
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The minimum amount for bulk packaging/pricing for this product is 1 x 1 ku. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
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