Papain References

Source:
Carica papaya Latex
CAS:
9001-73-4
EC:
3.4.22.2
Akahane, K. and Umeyama, H.
Alecio, M. , Dann, M. and Lowe, G.
Arnon, R.
Papain , Methods in Enzymology Vol. 19 , G. Perlmann and L. Lorand , Academic Press, NY , 226 , 1970
Azarkan, M. , El Moussaoui A. , van Wuytswinkel, D. , Dehon, G. and Looze, Y.
Fractionation and Purification of the Enzymes Stored in the Latex of Carica papaya ,
J Chromatogr B Analyt Technol Biomed Life Sci
790
, 229 , 2003
Balls, A. and Lineweaver, H.
Barrett, A. and Buttle, D.
Blumberg, S. , Schechter, I. and Berger, A.
Brisson, J. , Carey, P. and Storer, A.
Chaiken, I. and Smith, E.
Choudhury, D. , Roy, S. , Chakrabarti, C. , Biswas, S. and Dattagupta, J.
Clagett, J. and Englehard, W.
Coates, J. and Swann, J.
Cohen, L. , Fluharty, C. and Dihel, L.
Cohen, L. , Coghlan, V. and Dihel, L.
deJersey, J.
Dekeyser, P. , Smedt, S. , Demeester, J. and Lauwers, A.
Drenth, J. , Jansonius, J. , Koekoek, R. , Swen, H. and Wolters, B.
Finkbeiner, S. and Stevens, C.F.
Frater, R. , Light, A. and Smith, E.
Fujimoto, S. , Kanazawa, H. , Ishimitsu, S. and Ohara, A.
On the Mechanism of Inactivation of Papain by Hydroxylamine ,
Chem Pharm Bull (Tokyo)
38
, 546 , 1990
Glazer, A.
Glick, B. and Brubacher, L.
Hall, P. , Anderson, C. and Crawford, G.
Hill, R. and Smith, E.
,
Biochim Biophys Acta
19
, 376 , 1956
Iso, N. and Williams, J.
Kamphuis, I. , Kalk, K. , Swarte, M. and Drenth, J.
Kanazawa, H. , Fujimoto, S. and Ohara, A.
Kimmel, J. and Smith, E.
Kimmel, J. , Rogers, H. and Smith, E.
Liener, I.
The Sulfhydryl Proteases , Food Related Enzymes, Adv. in Chem. Series 136 , J. Whitaker , Amer. Chem. Soc., Washington, DC , 202 , 1974
Light, A. and Smith, E.
Chymotryptic Digest of Papain. I ,
J Biol Chem
235
, 3144 , 1960
Light, A. and Smith, E.
Chymotryptic Digest of Papain. II ,
J Biol Chem
235
, 3151 , 1960
Light, A. , Frater, R. , Kimmel, J. and Smith, E.
Light, A. , Glazer, A. and Smith, E.
Chymotryptic Digest of Papain. III ,
J Biol Chem
235
, 3159 , 1960
Lin, W. , Armstrong, D. and Gaucher, G.
Formation and Repair of Papain Sulfenic Acid ,
Can J Biochem
53
, 298 , 1975
Lindahl, P. , Alriksson, E. , Jšrnvall, H. and Bjork, I.
Liu, S. and Hanzlik, R.
Lowe, G. and Yuthavong, Y.
Maes, D. , Bouckaert, J. , Poortmans, F. , Wyns, L. and Looze, Y.
Martin, B. , Feinman, R. and Detwiler, T.
Matsumoto, K. , Murata, M. , Sumiya, S. , Kitamura, K. and Ishida, T.
Mitchel, R. , Chaiden, I. and Smith, E.
Moussaoui, A. , Nijs, M. , Paul, C. , Wintjens, R. , Vincentelli, J. , Azarkan, M. and Looze, Y.
Neumann, H. , Shinitzky, M. and Smith, R.
Nolan, C. and Smith, E.
Ozawa, K. , Ohnishi, T. and Tanaka, S.
Pickersgill, T. , Rizkallah, P. , Harris, G. and Goodenough, P.
Potter, J. , McCluskey, R. , Weissmann, G. and Thomas, L.
The Removal of Cartilage Matrix by Papain ,
J Exp Med
112
, 1173 , 1960
Revell, D. , Cummings, N. , Baker, K. , Collins, M. , Taylor, M. , Sumner, I. , Pickersgill, R. , Connerton, I. and Goodenough, P.
Schneider, M. and Goldstein, G.
Digestion of Articular Cartilage by Papain in Vitro ,
Proc Soc Exp Biol Med
124
, 991 , 1967
Schwert, G. , Neurath, H. , Kaufman, S. and Snoke, J.
The Specific Esterase Activity of Trypsin ,
J Biol Chem
172
, 221 , 1948
Shipton, M. , Kierstan, M. , Malthouse, J. , Stuchbury, T. and Brocklehurst, K.
Sluyterman, L.
The Activation Reaction of Papain ,
Biochim Biophys Acta
139
, 430 , 1967
Sluyterman, L. and DeGraaf, M.
Sluyterman, L. and Wijdenes, J.
Benzoylamido-acetonitrile as an Inhibitor of Papain ,
Biochim Biophys Acta
302
, 95 , 1973
Sluyterman, L. and Wijdenes, J.
Sluyterman, L. and Wijdenes, J.
Sluyterman, L.
Reversible Inactivation of Papain by Cyanate ,
Biochim Biophys Acta
139
, 439 , 1967
Sluyterman, L.
Smith, E. and Kimmel, J.
, The Enzymes, 2nd Ed. Vol. 4 , P. Boyer, H. Lardy, and K. Myrback , Academic Press, NY , 133 , 1960
Smith, E. , Kimmel, J. and Brown, P.
,
J Biol Chem
207
, 533 , 1954
Smith, L. , Garvin, P. , Gesler, R. and Jennings, R.
Solis-Mendiola, S. , Rojo-Dominguez, A. and Hernandez-Arana, A.
Storer, A. and Menard, R.
Taylor, M. , Pratt, K. , Revell, D. , Baker, K. , Sumner, I. and Goodenough, P.
Ungar-Waron, H. , Jaton, J. and Sela, M.
Action of Papain on Normal Rabbit Immunoglobulin M ,
Biochim Biophys Acta
140
, 542 , 1967
Valeri, A. , Wilson, S. and Feinman, R.
Van Eyk, H.
Fragmentation of Human Globulin with Papain ,
Biochim Biophys Acta
127
, 241 , 1966
Vernet, T. , Berti, P. , de Montigny, C. , Musil, R. , Tessier, D. , Macnard, R. , Magny, M. , Storer, A. and Thomas, D
Westerik, J. and Wolfenden, R.
Aldehydes as Inhibitors of Papain ,
J Biol Chem
247
, 8195 , 1972
Wolthers, B. , Dreuth, J. , Jansonius, J. , Koekoek, R. and Swen, H.
The Three Dimensional Structure of Papain , Structure-Function Relationships of Proteolytic Enzymes , P. Desnuelle, H. Neurath, and M. Ottesen , Academic Press, NY , 272 , 1970
Wolthers, B. and Kalk, K.
Zucker, S. , Buttle, D. , Nicklin, M. and Barrett, A.

Papain Products

Description
Activity
Code
Cat. #
Size
Price
Description
Papain, Suspension
Source:
Carica papaya Latex
Supplied as a two times crystalline suspension in 50mM sodium acetate, pH 4.5. To ensure full activity, the enzyme should be incubated in a solution containing 1.1mM EDTA, 0.067mM mercaptoethanol and 5.5mM cysteine-HCl for 30 minutes. It is recommended that the enzyme be 0.22 micron filtered after dissolution and prior to use.
Store at 2-8°C.
Ice Pack required
Activity
Activates to at least 20 units per mg protein
Code
PAP
LS003124
25 mg
$40.00
LS003126
100 mg
$88.00
LS003127
1 gm
$595.00
LS003128
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 20000 x 1 mg. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
Description
Papain, Lyophilized
Source:
Carica papaya Latex
Supplied as a lyophilized powder prepared from a two times crystalline suspension, Code: PAP. To ensure full activity, the enzyme should be incubated in a solution containing 1.1mM EDTA, 0.067mM mercaptoethanol and 5.5mM cysteine-HCl for 30 minutes. It is recommended that the enzyme be 0.22 micron filtered after dissolution and prior to use.
Store at 2-8°C.
Activity
Activates to at least 15 units per mg protein
Code
PAPL
LS003118
25 mg
$44.00
LS003119
100 mg
$100.00
LS003120
1 gm
$685.00
LS003122
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 20000 x 1 mg. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
Description
PDS Kit, Papain Vial
Source:
Papaya Latex
A component of the Papain Dissociation System, for use in the tissue dissociation method of Huettner, J.E., and Baughman, R.W.: J. Neuroscience, 6, 3044 (1986). Contains papain, L-cysteine, and EDTA. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 5 ml of EBSS or equivalent yields a solution at 20 units of papain per ml in 1mM L-cysteine with 0.5mM EDTA.
Store at 2-8°C.
Activity
≥100 units per vial
Code
PAP2
LK003176
1 vi
$29.00
LK003178
5 vi
$98.00