Ribonuclease T2, Recombinant - Manual

Source:
Aspergillus oryzae
CAS:
37278-25-4
EC:
4.6.1.19

Aspergillus oryzae Ribonuclease T2 is a member of the RNase T2 family of endonucleases that are present in a wide variety of microbial, plant and animal species. In contrast to Aspergillus oryzae Ribonuclease T1, which is an exclusively guanylic-acid specific endonuclease, all RNase T2-like enzymes are essentially base non-specific. However, RNase T2 endonucleases from different species can show slight base preferences. The fungal enzymes, including Aspargillus oryzae RNaseT2, show slight base preference in the following order: A>G>C, U. RNase T2 cleaves between the 3’-phosphate residue of one base and the 5’-OH residue of the adjacent nucleotide forming a 2’, 3’-cyclic phosphate intermediate followed by the generation of oligonucleotides with 3’-phosphate residues. RNase T2 has a molecular weight of 36 kDa and 12-15% of its mass is composed of carbohydrate. 

Characteristics of Ribonuclease T2, Recombinant
Optimal pH

4.5

Isoelectric point

5.0 

Activators
  • EDTA will stimulate activity, especially in the presence of divalent cations.
Inhibitors
  • Strongly inhibited by Cu2+, Zn2+ and Hg2+
  • Ca2+, Mg2+ and heparin (to a lesser degree)
  • Mononucleotides and RNase T2 digestion products (competitively)  

Applications: 

  • 3’ analysis of RNA and RNase protection assays

Ribonuclease T2, Recombinant Products

Description
Activity
Code
Cat. #
Price
Size
Description
Ribonuclease T2, Recombinant, Animal Free/AF
Source:
Aspergillus oryzae
Animal Free/AF. Recombinant aspergillus oryzae (non-mammalian) broad specificity Ribonuclease T2 highly purified & produced in Pichia pastoris prepared with non-animal components. Supplied as lyophilized powder.
Store at 2-8°C.
Activity
≥10,000 units per mg protein
Code
RT2R
Cat. #
LS01501