Proteinase K Product Information

Source: Tritirachium album limber


Native Proteinase K, product codes PROK and PROKS have been discontinued and superseded with Recombinant Proteinase K, product codes PROKR and PROKRS. The recombinant products meet the same specifications as the native products.

Proteinase K is a serine protease with broad specificity towards aliphatic, aromatic and other hydrophobic amino acids. PROK has a molecular weight of 27,000 daltons and is Ca2+ dependent. It is not inactivated by metal ion chelating agents such as EDTA, sulfhydryl reagents, PCMB, TLCK, or TPCK. It also retains activity in 0.5% SDS. It can be inhibited by PMSF or DFP.

Stability/Storage: The lyophilized powder is stable for one year at 2-8°C. Solutions in 50mM Tris-HCl, pH 8.0 with 1mM CaCl2 are stable for months at 2-8°C. Store at 2-8°C.

Unit Definition: One Unit releases one micromole of Folin positive amino acids, measured as tyrosine, at 37°C, pH 7.5, using urea denatured hemoglobin as the substrate.

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DescriptionActivityCodeSizeCat. No.Price
Proteinase K (Recombinant), Animal Free/AF
Animal Free/AF, Molecular Biology Grade purified recombinant Tritiachium album Proteinase K produced in yeast. Free of DNase and RNase. Supplied as a lyophilized powder. Store at 2-8°C.

Store at 2-8°C.
>20 units per mg dry weight PROKR 25 mg LS004248 $38.00 Buy Now
100 mg LS004249 $81.00 Buy Now
1 gm LS004250 $620.00 Buy Now
Bulk LS004252 Inquire
Proteinase K, (Recombinant) Solution
A concentrated, ready to use liquid formulation. Recombinant Proteinase K prepared at 20mg/ml in  10mM Tris-HCl, 1mM calcium acetate, pH 7.5 containing 50% glycerol. DNase- and RNase- free. Store at -20°C
Store at -20°C.
>400 u/ml PROKRS 5 ml LS004254 $120.00 Buy Now
25 ml LS004256 $480.00 Buy Now
Bulk LS004258 Inquire

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