Hyaluronidase - Manual

Source:
Bovine Testes
CAS:
37326-33-3
EC:
3.2.1.35

Hyaluronidase catalyzes the random hydrolysis of 1,4-linkages between 2-acetamido-2-deoxy-b-D-glucose and D-glucose residues in hyaluronate.

Characteristics of Hyaluronidase from Bovine Testes:

Specificity

Testicular hyaluronidase hydrolyzes the endo-N-acetylhexosaminic bonds of hyaluronic acid and chondroitin sulfuric acids A and C (but not B), primarily to tetrasaccharide residues (Ludowieg et al. 1961). Monosaccharides are not liberated (Rappaport et al. 1951).

Composition

The enzyme is a glycoprotein containing 5% mannose and 2.17% glucosamine. The amino acid composition has been determined ( Borders and Raftery 1968).

Characteristics of Hyaluronidase
Molecular Weight

Borders and Raftery (1968) reported a molecular weight of 61,000. Khorlin et al. (1973) report four subunits of 14,000 each and a total molecular weight of 55,000.

Optimal pH

4.5 - 6 (DeSalequi et al. 1967).

Extinction Coefficient

extinction coefficient= approximately 8.

Inhibitors

Fe2+ and Fe3+ are inhibitory as are Mn2+ and Cu2+ (Warren et al. 1962).

Stability

Worthington hyaluronidase is stable for 1 - 2 years and the purified preparation for 6 - 12 months when stored at -20°C.

Stabilizers

Yang and Srivastav (1975) report that sodium chloride acts as a stabilizer.

Hyaluronidase Products

Description
Activity
Code
Cat. #
Size
Price
Description
Hyaluronidase, Purified
Source:
Bovine Testes
Chromatographically purified. A dialyzed, lyophilized powder.
Store at -20°C.
Activity
≥3,000 USP/NF units per mg dry weight
Code
HSEP
LS005477
5 ku
$89.00
LS005475
15 ku
$220.00
LS005474
30 ku
$352.00
LS005479
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 600 x 1 ku. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
Description
Hyaluronidase
Source:
Bovine Testes
A partially purified, dialyzed, lyophilized powder.
Store at -20°C.
Activity
≥300 USP/NF units per mg dry weight
Code
HSE
LS002594
50 ku
$55.00
LS002592
300 ku
$230.00
LS002591
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 6000 x 1 ku. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.