Protease, Staph aureus (Endoproteinase Glu-C)

Source:
Staphylococcus aureus V8
CAS:
66676-43-5
EC:
3.4.21.19

I.U.B.: 3.4.21.19

Protease S. aureus V8 (Endoproteinase-Glu-C) specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acids. In the presence of ammonium, the enzyme specificity is limited to glutamic sites. It has a molecular weight of 27,000 daltons and optimum pH's of 4.0 and 7.8 with hemoglobin as the substrate. Protease S. aureus V8 is inhibited by diisopropylfluorophosphate and monovalent anions such as F-, Cl-, CH3COO- and NO3. Enzyme activity is determined by the casein digestion assay described by Drapeau (Methods Enzymol., 45, 469, 1976).

Stability/Storage: Autolysis occurs at temperatures greater than 40°C. The enzyme is fully active in USP 0.2% SDS. Stable for 12 months at 2-8°C.

Related Products:

Carboxypeptidase B (COBC/COBPMS)
Carboxypeptidase Y (COY)Chymotrypsin (CDAG/CDI/CDS/CDTLCK)
Clostripain, Endo-Arg-C (CP)
Neutral Protease (Dispase, NPRO)
Proteinase K (PROK)
Trypsin (TL/TRL/TRL3/TRLS/TRTPCK)
Trypsin, SequENZ (TRSEQII/TRSEQZ)

Unit Definition
One Unit causes a change of 0.001 A280 nm per minute at 37°C, pH 7.8 using casein as the substrate.

Protease, Staph aureus (Endoproteinase Glu-C) Products

Description
Activity
Code
Cat. #
Price
Size
Description
Protease, S. aureus, Sequencing Grade
Source:
Staphylococcus aureus V8
Chromatographically purified according to Drapeau, G, et.al, JBC, 247, 6720 (1972). Supplied as a lyophilized powder containing 10µg or 50µg protein.
Store at 2-8°C.
Code
STSEQ
Cat. #
LS02126
Description
Protease, S. Aureus (Endoproteinase Glu-C)
Source:
Staphylococcus aureus V8
Chromatographically purified according to Drapeau, G., Boily, Y., and Houmard, J.: JBC, 247, 6720 (1972). A lyophilized powder.
Store at 2-8°C.
Activity
≥500 units per mg dry weight
Code
STAP
Cat. #
LS003608