Protease, Staph aureus (Endoproteinase Glu-C) - Manual

Source:
Staphylococcus aureus V8
CAS:
66676-43-5
EC:
3.4.21.19

Protease S. aureus, V8 (endoproteinase-Glu-C) specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acids (Drapeau et al. 1972). Houmard and Drapeau (1972) report that in the presence of ammonium buffers the enzyme specificity can be limited to glutamoyl bonds. Its rather unique specificity, which can be considered as opposite to that of trypsin, makes it a useful tool for protein chemistry and peptide mapping studies (Cleveland et al. 1977) and (Hall et al. 1978).

Characteristics of Protease from S. aureus, V8:

Characteristics of Protease, Staph aureus (Endoproteinase Glu-C)
Molecular Weight

27,000 (Drapeau 1978).

Optimal pH

4.0 and 7.8 with hemoglobin substrate. (Drapeau et al. 1972).

Extinction Coefficient

extinction coefficient = 4.26 (Houmard 1976).

Inhibitors

Diisopropyl fluorophosphate (DFP) and monovalent anions such as F-, Cl-, Br-, CH3COO-, and NO3- (Houmard 1976).

Protease, Staph aureus (Endoproteinase Glu-C) Products

Description
Activity
Code
Cat. #
Size
Price
Description
Protease, S. aureus, Sequencing Grade
Source:
Staphylococcus aureus V8
Chromatographically purified according to Drapeau, G, et.al, JBC, 247, 6720 (1972). Supplied as a lyophilized powder.
Store at 2-8°C.
Code
STSEQ
LS02126
5x10 ug
$192.00
LS02128
5x50 ug
$520.00
LS02129
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 5000 x 1 ug. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
Description
Protease, S. Aureus (Endoproteinase Glu-C)
Source:
Staphylococcus aureus V8
Chromatographically purified according to Drapeau, G., Boily, Y., and Houmard, J.: JBC, 247, 6720 (1972). A lyophilized powder.
Store at 2-8°C.
Activity
≥500 units per mg dry weight
Code
STAP
LS003608
1 mg
$76.00
LS003605
5 mg
$270.00
LS003606
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 100 x 1 mg. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.