Trypsin References

Source:
Bovine Pancreas
CAS:
9002-07-7
EC:
3.4.21.4
Abuchowski, A. and Davis, F.
Alagiri, S. and Singh, T.
Antonini, E. and Ascenzi, P.
Appavou, M. , Gibrat, G. and Bellissent-Funel, M.
Arakawa, T. , Hung, L. , McGinley, M. , Rohde, M. and Narhi, L.
Arnon, R. and Neurath, H.
An Immunological Approach to the Study of Evolution of Trypsins ,
Proc Natl Acad Sci U S A
64
, 1323 , 1969
Ascenzi, P. , Amiconi, G. , Bolognesi, M. , Menegatti, E. and Guarneri, M.
Ascenzi, P. , Bolognesi, M. , Guarneri, M. , Menegatti, E. and Amiconi, G.
Ascenzi, P. , Menegatti, E. , Bortolotti, F. , Guarneri, M. and Antonini, E.
Ashton, D. , Ashcroft, A. , Beddell, C. , Green, B. and Oliver, R.
Balls, A.
Concerning Trypsinogen ,
Proc Natl Acad Sci U S A
53
, 392 , 1965
Banumathi, E. , Haribalaganesh, R. , Babu, S. , Kumar, N. and Sangiliyandi, G.
Bergmann, M. and Fruton, J.
, Advances in Enzymology Vol. 63 , 1941
Berliner, L. and Wong, S.
Bricteux-Gregoire, S. , Schyns, R. and Florkin, M.
Castaneda-Agullo, M. and Del Castillo, L.
Chambers, J. , Christoph, G. , Krieger, M. , Kay, L. and Stroud, R.
Chen, N. , Zou, J. , Wang, S. , Ye, Y. , Huang, Y. , Gadda, G. and Yang, J.
Cohen, W. , Lache, M. and Erlanger, B.
Coletta, M. , Ascenzi, P. , Amiconi, G. , Bolognesi, M. , Guarneri, M. and Menegatti, E.
Conners, R. , Konarev, A. , Forsyth, J. , Lovegrove, A. , Marsh, J. , Joseph-Horne, T. , Shewry, P. and Brady, R.
Craik, C. , Roczniak, S. , Largman, C. and Rutter, W.
D'Angelo, M. , Visintin, J. , Richtzenhain, L. and Goncalves, R.
Delaage, M. and Lazdunski, M.
DelCastillo, L. , Davila, G. , Dorantes, L. , Oliver, C. , Ibarra, R. and Castaneda-Agullo, M.
Desnuelle, P.
Trypsin , The Enzymes Vol. 4 , P. Boyer, H. Lardy, and K. Myrback , Academic Press, NY , 119 , 1960
Dominko, T. , Ramalho-Santos, J. , Chan, A. , Moreno, R. , Luetjens, C. , Simerly, C. , Hewitson, L. , Takahashi, D. , Martinovich, C. , White, J. and Schatten, G.
Finer-Moore, J. , Kossiakoff, A. , Hurley, J. , Earnest, T. and Stroud, R.
Solvent Structure in Crystals of Trypsin Determined by X-Ray and Neutron Diffraction ,
Proteins, Structure Function & Genetics
12
, 203 , 1992
Ford, J. , Chambers, R. and Cohen, W.
An Active-site Titration Method for Immobilized Trypsin ,
Biochim Biophys Acta
309
, 175 , 1973
Fraenkel-Conrat, H. , Bean, R. and Lineweaver, H.
Fromherz, P. and Marcheva, D.
Gabel, D. , Vretblad, P. , Axen, R. and Porath, J.
Insolubilized Trypsin with Activity in 8 M Urea ,
Biochim Biophys Acta
214
, 561 , 1970
Ghiron, C. , Longworth, J. and Ramachandran, J.
Triplet-Triplet Energy Transfer in alpha-Trypsin ,
Proc Natl Acad Sci U S A
70
, 3703 , 1973
Glenner, G. , Hopsu, V. and Cohen, L.
New Substrates of Trypsin and Some Trypsin-like Enzymes ,
J Histochem Cytochem
12
, 545 , 1964
Graf, L. , Craik, C. , Patthy, A. , Roczniak, S. , Fletterick, R. and Rutter, W.
Graf, L. , Hegyi, G. , Liko, I. , Hep, J. , Medzihradszky, K. , Craik, C. and Rutter, W.
Green, M. , Gladner, J. , Cunningham, L. and Neurath, H.
Green, S. and Neurath, H.
Proteolytic Enzymes , The Proteins Vol. II , H. Neurath , Academic Press, NY , 1057 , 1954
Greenbaum, L. , Hirshkowitz, A. and Schoichet, I.
The Activation of Trypsinogen by Cathepsin B ,
J Biol Chem
234
, 2885 , 1959
Griffiths, A. and Brechner, A.
Hartley, B.
Homologies in Serine Proteinases ,
Phil Trans R Soc Lond
257
, 77 , 1970
Helmqvist, B. , Borgstrs<caron>m, A. and Ohlsson, K.
Hermodson, M. , Ericsson, L. , Neurath, H. and Walsh, K.
Higaki, J. and Light, A.
Holeysovsky, V. , Keil, B. and Sorm, F.
Isackson, P. , Beaudoin, J. , Hermodson, M. and Reeck, G.
Iwaki, K. , Ogawa, M. , Kitahara, T. , Tanaka, S. and Kosaki, G.
Jones, R.
Jurasek, L. and Smillie, L.
Kawasaki, H. , Kurosu, Y. , Kasai, H. , Isobe, T. and Okuyama, T.
Kay, C. , Smillie, L. and Hilderman, F.
The Molecular Weight of Trypsinogen ,
J Biol Chem
236
, 118 , 1961
Keil-Dlouha, V. , Zylber, N. , Imhoff, J. , Tong, N. and Keil, B.
Proteolytic Activity of Pseudotrypsin ,
F.E.B.S. Lett.
16
, 291 , 1971
Keil-Dlouha, V. , Zylber, N. , Tong, N. and Keil, B.
Cleavage of Glucagon by alpha- and beta-Trypsin ,
F.E.B.S. Lett.
16
, 287 , 1971
Kossiakoff, A. , Chambers, J. , Kay, L. and Stroud, R.
Koutsopoulos, S. , Patzsch, K. , Bosker, W. and Norde, W.
Kunugi, S. , Fukuda, M. and Ise, N.
Liepinsh, E. and Otting, G.
Light, A. and Fonseca, P.
Liu, K. , Markakis, P. and Smith, D
Luong, J. , Male, K. and Nguyen, A.
Luong, J. , Male, K. and Nguyen, A.
Mannhalter, C. , Schiffman, S. and Jacobs, A.
Trypsin Activation of Human Factor XI ,
J Biol Chem
255
, 2667 , 1980
Markwardt, F. , Richter, M. , Walsmann, P. and Landmann, H.
Maroux, S. , Rovery, M. and Desnuelle, P.
Menegatti, E. , Guarneri, M. , Bolognesi, M. , Ascenzi, P. and Amiconi, G.
Mitin, Y. , Zapevalova, N. and Gorbunova, E.
Mohan, S. , Jennings, J. , Linkhart, T. and Baylink, D.
Morgan, P. , Walsh, A. and Neurath, H.
Moroi, M. and Yamasaki, M.
Muller, O. and Yamanouchi, I.
Murray, P. , Silberstein, S. , Cantore, M. and Passeron, S.
Neurath, H. and Schwert, G.
Crystalline Proteolytic Enzymes ,
Chem Rev
46
, 69 , 1950
Neurath, H.
The Activation of Zymogens ,
Adv Protein Chem
12
, 319 , 1957
Northrop, J. and Kunitz, M.
Northrop, J. , Kunitz, M. and Herriott, R.
, Crystalline Enzymes , Columbia Univ Press , 125 , 1948
Odorzynski,T. and Light, A.
Ohlsson, K. and Tegner, H.
Ohshima, Y. , Suzuki, Y. , Nakatani, A. and Nohara, D.
Perlmutter, D. , Joslin, G. , Nelson, P. , Schasteen, C. , Adams, S. and Fallon, R.
Pitotti, A. , Maurich, V. , Moneghini, M. , Franchi, G. and Lencioni, E.
Polanowski, A. , Cieslar, E. , Otlewski, J. , Nienartowicz, B.,Wilimowska-Pelc, A. and Wilusz, T.,
Read, R. , Brayer, G. , Jurasek, L. and James, M.
Reeck, G. and Neurath, H.
Reichart, E. , Boerkmann, P. , Vignaud, J. and Plenat, F.
Rice, R. , Means, G. and Brown, W.
Stabilization of Bovine Trypsin by Reductive Methylation. ,
Biochim Biophys Acta
492
, 316-21 , 1977
Ridderknecht, H. , Fleming, R. and Geokus, M.
Sarkany, R. and Moreland, B.
Sattar, S. , Abbas, B. , Jones, L. , Saridogan, E. , Mahmood, T. , Mehta, J. and Djahanbakhch, O.
Sawada, H. , Kawahigashi, M. , Yokosawa, H. and Ishii, S.
Schaffer, N. , Engle, R. , Simet, L. , Drisko, D. and Harshman, S.
Schilling, R. and Mitra, A.
Shtelzer, S. , Rappoport, S. , Avnir, D. , Ottolenghi, M. and Braun, S.
Smillie, L. and Neurath, H.
Smith, R. and Shaw, E.
Tallan, H.
Chromatography of Trypsinogen ,
Biochim Biophys Acta
27
, 407 , 1958
Terminiello, L. , Ram, S. , Bier, M. and Nord, F.
Tietze, F.
Ueno, Y. and Morihara, K.
Vandermeers, A. , Vanaermeers-Piret, M. , Rathe, J. and Christophe, J.
Walde, P. , Peng, Q. , Fadnavis, N. , Battistel, E. and Luisi, P.
Walsh, K. and Neurath, H.
Trypsinogen and Chymotrypsinogen as Homologous Proteins ,
Proc Natl Acad Sci U S A
52
, 884 , 1964
Walsh, K. , Kauffman, D. , Sampath Kumar, K. and Neurath, H.
Walsh, K.
Trypsinogens and Trypsins of Various Species , Methods in Enzymology Vol. 19 , G. Perlmann and L. Lorand , Academic Press, NY , 41 , 1970
Wang, Q. , Zheng, Y. , Guo, X. and Wang, S.
Watanabe, T. and Ogasawara, N.
Willstatter, R. , Waldschmidt-Leitz, E. , Dunaiturria, S. and Kunstner, G.
,
Hoppe-Seylers Z Physiol Chem
161
, 191 , 1926
Woodward, C. , Ellis, L. and Rosenberg, A.
Yamashina, I.
The Action of Enterokinase on Trypsinogen ,
Biochim Biophys Acta
20
, 433 , 1956
Yang, L. , Dong, W. , He, J. , Ren, X. and Yan, W.
Zhou, J. , Liu, C. and Tsou, C.

Trypsin Products

Description
Activity
Code
Cat. #
Size
Price
Description
SequENZ® Trypsin, Modified, Sequencing Grade
Source:
Bovine Pancreas
Trypsin, treated with L-(tosylamido-2-phenyl) ethyl chloromethyl ketone to inhibit contaminating chymotryptic activity, chemically modified to promote stability and further purified to remove autolysis fragments, resulting in a highly stable trypsin product resistant to autolysis while retaining specificity.
Store at -20°C. PROTECT FROM MOISTURE.
Ice Pack required
Activity
≥150 units per mg protein (at least 8,625 BAEE/2875 USP/NF units per mg protein)
Code
TRSEQZ
LS02120
4x25 µg
$94.00
LS02122
4x100 µg
$265.00
LS02123
1 mg
$480.00
LS02124
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 20000 x 1 ug. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
Description
SequENZ® Trypsin, Modified, Sequencing Grade, Solution
Source:
Bovine Pancreas
Ready to use liquid preparation of Trypsin, treated with L-(tosylamido-2-phenyl) ethyl chloromethyl ketone to inhibit contaminating chymotryptic activity, chemically modified to promote stability and further purified to remove autolysis fragments, resulting in a highly stable trypsin product resistant to autolysis while retaining specificity. Supplied as 1mgP/ml in 1.0mM HCl.
Store at 2-8°C.
Ice Pack required
Code
TRSEQZS
LS02150
250 ug
$125.00
LS02152
1000 ug
$400.00
LS02155
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 20000 x 1 ug. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
Description
Trypsin, Purified, Sequencing Grade II
Source:
Bovine Pancreas
Bovine trypsin that has been treated with L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK) to inhibit contaminating chymotryptic activity and extensively purified to remove autolysis products. Supplied as a lyophilized powder.
Store at -20°C. PROTECT FROM MOISTURE.
Ice Pack required
Activity
≥150 units per mg protein (at least 8,625 BAEE/2875 USP/NF units per mg protein)
Code
TRSEQII
LS02115
4x25 µg
$82.00
LS02117
4x100 µg
$219.00
LS02119
1 mg
$405.00
LS02118
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 20000 x 1 µg. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
Description
Trypsin, TPCK Treated
Source:
Bovine Pancreas
A chromatographically purified, diafiltered, lyophilized powder that has been treated with L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK) to inhibit contaminating chymotryptic activity [Kostka, V., and Carpenter, F.: JBC, 239, 1799 (1964)].
Store at 2-8°C. PROTECT FROM MOISTURE.
Activity
≥180 units per mg protein (10,350 BAEE/3,450 USP/NF units per mg protein)
Code
TRTPCK
LS003740
100 mg
$112.00
LS003741
500 mg
$400.00
LS003744
1 gm
$675.00
LS003742
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 20000 x 1 mg. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
Description
Trypsin
Source:
Bovine Pancreas
Supplied as a chromatographically purified, diafiltered and lyophilized powder.
Store at 2-8°C. PROTECT FROM MOISTURE.
Activity
≥180 units per mg protein
Code
TRL3
LS003708
100 mg
$52.00
LS003707
1 gm
$325.00
LS003709
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 20000 x 1 mg. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
Description
Trypsin
Source:
Bovine Pancreas
Supplied as a dialyzed and lyophilized powder.
Store at 2-8°C. PROTECT FROM MOISTURE.
Activity
≥180 units per mg protein
Code
TRL
LS003702
100 mg
$28.00
LS003703
1 gm
$153.00
LS003704
10 gm
$1,110.00
LS003706
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 200000 x 1 mg. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
Description
Trypsin, TPCK-Treated, Irradiated
Source:
Bovine Pancreas
Chromatographically purified trypsin treated with L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK) to inhibit contaminating chymotryptic activity according to Kostka, V., and Carpenter, F.H.: JBC, 239, 1799 (1964), Code: TRTPCK, lyophilized, irradiated and tested for the absence of mycoplasma and extraneous virus according to 9 CFR 113.53c. Each vial is filled to contain 100 mg.
Store at 2-8°C.
Activity
≥180 units per mg protein
Code
TRTVMF
LS003750
100 mg
$170.00
LS003752
5x100 mg
$685.00
Description
Trypsin, 2X, Sterile, Irradiated
Source:
Bovine Pancreas
Purified trypsin (Code: TRL), lyophilized, irradiated and tested for the absence of mycoplasma and extraneous virus according to 9 CFR113.53c. Each vial is filled to contain 100 mg.
Store at 2-8°C.
Activity
≥180 units per mg protein (10,350 BAEE/3,450 USP/NF units per mg protein)
Code
TRLVMF
LS004454
100 mg
$109.00
LS004452
5x100 mg
$399.00
LS004458
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 10000 x 1 mg. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
Description
Trypsin, 0.22µ Filtered
Source:
Bovine Pancreas
Trypsin chromatographically purified, diafiltered (Code TRL3) filtered thorugh a 0.22 micron pore size membrane and lyophilized in sterile vials. This product is not tested for pyrogenicity.
Store at 2-8°C.
Activity
≥180 units per mg protein (10,350 BAEE/3,450 USP/NF units per mg protein)
Code
TRLS
LS003736
50 mg
$52.00
LS003734
5x50 mg
$225.00
LS003738
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 100 x 50 mg. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.