Chymotrypsin

Source:
Bovine Pancreas
CAS:
9004-07-3
EC:
3.4.21.1

Chymotrypsin preferentially catalyzes the hydrolysis of peptide bonds involving L-isomers of tyrosine, phenylalanine and tryptophan. It also readily acts upon amides and esters of susceptible amino acids. Chymotrypsin catalyzes the hydrolysis of bonds of leucyl, methionyl, asparaginyl and glutamyl residues.

Stability/Storage: The enzyme is stable for days in solution at pH 3.0 and for years as a dry powder at 2-8°C. Protect from moisture.

1 BTEE unit = 29.5 USP/NF units.

Related Products:

Carboxypeptidase B (COBC/COBPMS)
Carboxypeptidase Y (COY)Clostripain, Endo-Arg-C (CP)
Collagenase (CLS1-4/CLSPA)
Elastase (ES/ESL/ESFF)
Hyaluronidase (HSE/HSEP)
Neutral Protease (Dispase, NPRO)
Papain (PAP/PAPL/PAP2)
Pepsin (PM)
Protease, Staph Aureus, Endo-Glu-C (STAP)
Proteinase K (PROK)
Trypsin (TL/TRL/TRL3/TRLS/TRTPCK)
Trypsin, SequENZ (TRSEQII/TRSEQZ)
Trypsin Inhibitors (LBI/OI/SI/SIC)
Unit Definition
One Unit hydrolyzes one micromole of benzoyl-L-tyrosine ethyl ester per minute at 25°C, pH 7.8 in the presence of calcium. An activity of 45 units per mg using the above definition, is the equivalent of 10,000 optical density or 1330 N.F. units per mg using ATEE as a substrate.

Chymotrypsin Products

Description
Activity
Code
Cat. #
Price
Size
Description
Chymotrypsin, Alpha, TLCK Treated, Sequencing Grade
Source:
Bovine Pancreas
Three time crystallized and treated with 1-chloro-3-tosylamido-7-amino-2-heptanone (TLCK) to inhibit trypsin activity [Shaw, et al., Biochemistry, 4, 2219 (1965)]. Dialyzed to remove autolysis products and low molecular weight contaminants. Supplied as lyophilized powder.
Store at 2-8°C.
Activity
≥40 u/mgP
Code
CDSEQ
Cat. #
LS02130
Description
Chymotrypsin, Alpha, TLCK Treated
Source:
Bovine Pancreas
Three times crystallized and treated with 1-chloro-3-tosylamido-7-amino-2-heptanone (TLCK) to inhibit trypsin activity [Shaw, et al., Biochemistry, 4, 2219 (1965)]. Dialyzed against 1 mM HCl to remove autolysis products and low molecular weight contaminants. Supplied as a dialyzed, lyophilized powder.
Store at 2-8°C.
Activity
≥45 units per mg protein
Code
CDTLCK
Cat. #
LS001430
Description
Chymotrypsin, Alpha, Purified
Source:
Bovine Pancreas
Chromatographically prepared by the procedure of Yapel, et al., J. Amer. Chem. Soc., 88, 2573 (1966). A lyophilized powder.
Store at 2-8°C.
Activity
≥45 units per mg protein
Code
CDS
Cat. #
LS001475
Description
Chymotrypsin, Alpha
Source:
Bovine Pancreas
Three times crystallized alpha chymotrypsin which is an activation product of a 3X crystallized zymogen. Dialyzed against 1mM HCl and lyophilized.
Store at 2-8°C.
Activity
≥45 units per mg protein
Code
CDI
Cat. #
LS001448
Description
Chymotrypsin, Alpha
Source:
Bovine Pancreas
Crystallized as zymogen and activated. Dialyzed against 1mM HCl and lyophilized.
Store at 2-8°C.
Activity
≥35 units per mg protein
Code
CDAG
Cat. #
LS001333