Chymotrypsin

Source:
Bovine Pancreas
CAS:
9004-07-3
EC:
3.4.21.1

Chymotrypsin preferentially catalyzes the hydrolysis of peptide bonds involving L-isomers of tyrosine, phenylalanine and tryptophan. It also readily acts upon amides and esters of susceptible amino acids. Chymotrypsin catalyzes the hydrolysis of bonds of leucyl, methionyl, asparaginyl and glutamyl residues.

Stability/Storage: The enzyme is stable for days in solution at pH 3.0 and for years as a dry powder at 2-8°C. Protect from moisture.

1 BTEE unit = 29.5 USP/NF units.

Related Products:

Carboxypeptidase B (COBC/COBPMS)
Carboxypeptidase Y (COY)Clostripain, Endo-Arg-C (CP)
Collagenase (CLS1-4/CLSPA)
Elastase (ES/ESL/ESFF)
Hyaluronidase (HSE/HSEP)
Neutral Protease (Dispase, NPRO)
Papain (PAP/PAPL/PAP2)
Pepsin (PM)
Protease, Staph Aureus, Endo-Glu-C (STAP)
Proteinase K (PROK)
Trypsin (TL/TRL/TRL3/TRLS/TRTPCK)
Trypsin, SequENZ (TRSEQII/TRSEQZ)
Trypsin Inhibitors (LBI/OI/SI/SIC)
Unit Definition
One Unit hydrolyzes one micromole of benzoyl-L-tyrosine ethyl ester per minute at 25°C, pH 7.8 in the presence of calcium. An activity of 45 units per mg using the above definition, is the equivalent of 10,000 optical density or 1330 N.F. units per mg using ATEE as a substrate.

Chymotrypsin Products

Description
Activity
Code
Cat. #
Size
Price
Description
Chymotrypsin, Alpha, TLCK Treated, Sequencing Grade
Source:
Bovine Pancreas
Three time crystallized and treated with 1-chloro-3-tosylamido-7-amino-2-heptanone (TLCK) to inhibit trypsin activity [Shaw, et al., Biochemistry, 4, 2219 (1965)]. Dialyzed to remove autolysis products and low molecular weight contaminants. Supplied as lyophilized powder.
Store at 2-8°C.
Activity
≥40 u/mgP
Code
CDSEQ
LS02130
4x25 ug
$180.00
LS02132
4x100 ug
$472.00
Description
Chymotrypsin, Alpha, TLCK Treated
Source:
Bovine Pancreas
Three times crystallized and treated with 1-chloro-3-tosylamido-7-amino-2-heptanone (TLCK) to inhibit trypsin activity [Shaw, et al., Biochemistry, 4, 2219 (1965)]. Dialyzed against 1 mM HCl to remove autolysis products and low molecular weight contaminants. Supplied as a dialyzed, lyophilized powder.
Store at 2-8°C.
Activity
≥45 units per mg protein
Code
CDTLCK
LS001430
25 mg
$31.00
LS001432
100 mg
$75.00
LS001434
1 gm
$584.00
LS001438
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 20000 x 1 mg. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
Description
Chymotrypsin, Alpha, Purified
Source:
Bovine Pancreas
Chromatographically prepared by the procedure of Yapel, et al., J. Amer. Chem. Soc., 88, 2573 (1966). A lyophilized powder.
Store at 2-8°C.
Activity
≥45 units per mg protein
Code
CDS
LS001475
100 mg
$76.00
LS001479
1 gm
$578.00
LS001477
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 20000 x 1 mg. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
Description
Chymotrypsin, Alpha
Source:
Bovine Pancreas
Three times crystallized alpha chymotrypsin which is an activation product of a 3X crystallized zymogen. Dialyzed against 1mM HCl and lyophilized.
Store at 2-8°C.
Activity
≥45 units per mg protein
Code
CDI
LS001448
250 mg
$44.00
LS001450
1 gm
$127.00
LS001451
10 gm
$955.00
LS001453
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 200000 x 1 mg. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
Description
Chymotrypsin, Alpha
Source:
Bovine Pancreas
Crystallized as zymogen and activated. Dialyzed against 1mM HCl and lyophilized.
Store at 2-8°C.
Activity
≥35 units per mg protein
Code
CDAG
LS001333
1 gm
$38.00
LS001334
10 gm
$225.00
LS001332
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 200 x 1 gm. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.