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Tissue References Search Tool

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Liver
Fish
Rainbow trout (Oncorhynchus mykiss), male & female,
120-600 g
Blair, J.B. , Miller, M.R. , Pack, D. , Barnes, R. , Teh, S.J. and Hinton, D.E.
Isolated Trout Liver Cells:Establishing Short-Term Primary Cultures Exhibiting Cell-to-Cell Interactions ,
In Vitro Cell Dev Biol
 26 , 237 , 1990
Cells
Hepatocytes
Enzymes
Medium
Kreb's-Ringer bicarbonate buffer, CF
Liver
Fish
Trout, male, 100 - 200 g
Lipsky, M. , Sheridan, T. , Bennett, R. and May, E.
Comparison of Trout Hepatocyte Culture on Different Substrates ,
In Vitro Cell Dev Biol
 22 , 360 , 1986
Cells
Hepatocytes
Enzymes
Medium
HBSS with 0.05M HEPES
Liver
Fish
Rainbow trout, (Salmo gairdneri), male, 150-200 g
Klaunig, J. , Ruch, R. and Goldblah, P.
Trout Hepatocyte Culture: Isolation and Primary Culture ,
In Vitro Cell Dev Biol
 21 , 221 , 1985
Cells
Hepatocytes
Enzymes
Collagenase : 100 u/ml
Medium
HBSS/CMF
Liver
Fish
Trout (Salmo gairdneri), male, 150-200 g
Catfish (Ictalurus punctatus), male, 100 - 150 g
Klaunig, J.
Establishment of Fish Hepatocyte Cultures For Use in In Vitro Carcinogenicity Studies ,
Natl Cancer Inst Monogr
 65 , 163 , 1981
Cells
Hepatocytes
Enzymes
Collagenase : 100u/ml
Medium
HBSS
Liver
Guinea-Pig
Guinea-pig, Hartley, male, 250-300 g
Arinze, I. and Kawai, Y.
Adrenergic Regulation of Glycogenolysis in Isolated Guinea-Pig Hepatocytes: Evidence that B2-Receptors Mediate Catecholamine Stimulation of Glycogenolysis ,
Arch Biochem Biophys
 225 , 196 , 1983
Cells
Hepatocytes
Medium
Kreb's Ringer bicarbonate buffer
Liver
Guinea-Pig
Guinea-pig, young
Reese, J. and Byard, J.
Isolation And Culture of Adult Hepatocytes from Liver Biopsies ,
In Vitro
 17 , 935 , 1981
Cells
Hepatocytes
Enzymes
Collagenase : 90 u/ml
Medium
CF EGTA perfusate
Liver
Human

Human

Kucykowicz, S. , Amin, O. , Burton, A. , Swadling, L. , Schmidt, N. , Zakeri, N. , Davies, J. , Aidoo-Micah, G. , Stegmann, K. , Easom, N. , Jeffery-Smith, A. , Maini, M. and Pallett, L.
Isolation of Human Intrahepatic Leukocytes for Phenotypic and Functional Characterization by Flow Cytometry. ,
Star Protocols
 3 , 101356 , 2022
Cells
Intrahepatic leukocytes
Enzymes
Medium
RPMI-1640
Liver
Human
Human
Reuschel, E, Jilg, W. , Seelbach-Goebel, B. and Demi, L.
Comparative Purification and Characterization of Hepatitis B Virus-like Particles Produced by Recombinant Vaccinia Viruses in Human Hepatoma Cells and Human Primary Hepatocytes ,
PLoS ONE
 , 2019
Cells
Hepatocytes
Enzymes
Medium
HBSS
Liver
Human
Human
Pfeiffer, E. , Kegel, V. , Zeilinger, K. , Hengstler, J. , Nussler, A. , Seehofer, D. and Damm, G.
Featured Article: Isolation, Characterization, and Cultivation of Human Hepatocytes and Non-Parenchymal Liver Cells. ,
Exp Biol Med (Maywood)
 240 , 645-56 , 2015
Cells
Hepatocytes and non-parenchymal cells
Enzymes
Medium
HBSS
Liver
Human
Human
Werner, M. , Driftmann, S. , Kleinehr, K. , Kaiser, G. , Mathe, Z. , Treckmann, J. , Paul, A. , Skibbe, K. , Timm, J. , Canbay, A. , Gerken, G. , Schlaak, J. and Broering, R.
All-In-One: Advanced Preparation of Human Parenchymal and Non-Parenchymal Liver Cells. ,
PLoS ONE
 10 , e0138655 , 2015
Cells
Hepatocytes
Enzymes
Medium
DMEM
Liver
Human
Human
Lee, S. , Schelcher, C. , Demmel, M. , Hauner, M. and Thasler, W.
Isolation of Human Hepatocytes by a Two-Step Collagenase Perfusion Procedure. ,
J Vis Exp
 , 2013
Cells
Hepatocytes
Enzymes
Collagenase : 0.1-0.15%
Medium
HEPES
Liver
Human
Human, fetal
Vatakis, D. , Bristol, G. , Kim, S. , Levin, B. , Liu, W. , Radu, C. , Kitchen, S. and Zack, J.
Using the BLT Humanized Mouse as a Stem Cell Based Gene Therapy Tumor Model. ,
J Vis Exp
 70 , e4181 , 2012
Cells
Liver hematopoietic
Enzymes
Medium
RPMI
Liver
Human
Human
Torok, E. , Lutgehetmann, M. , Bierwolf, J. , Melbeck, S. , Dullmann, J. , Nashan, B. , Ma, P. and Pollok, J.
Primary Human Hepatocytes on Biodegradable Poly(l-lactic acid) Matrices: A Promising Model for Improving Transplantation Efficiency with Tissue Engineering. ,
Liver Transpl
 17 , 104 , 2011
Cells
Hepatocytes
Enzymes
Medium
Williams E
Liver
Human
Human
Wauthier, E. , Schmelzer, E. , Turner, W. , Zhang, L. , LeCluyse, E. , Ruiz, J. , Turner, R. , Furth, M. , Kubota, H. , Lozoya, O. , Barbier, C. , McClelland, R. , Yao, H. , Moss, N. , Bruce, A. , Ludlow, J. and Reid, L.
Hepatic Stem Cells and Hepatoblasts: Identification, Isolation, and Ex Vivo Maintenance. ,
Methods Cell Biol
 86 , 137 , 2008
Cells
Hepatic stem cells and heptoblasts
Enzymes
Collagenase Type 4 : 0.014-0.06%
Medium
various
Liver
Human
Human
Pichard L, Raulet E, Fabre G, Ferrini JB, Ourlin JC. and Maurel P
Human hepatocyte culture ,
Methods Mol Biol
 320 , 283 , 2006
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
HEPES
Liver
Human
Human
Hughes, R. , Mitry, R. , Dhawan, A. , Lehec, S. , Girlanda, R. , Rela, M. , Heaton, N. and Muiesan, P.
Isolation of Hepatocytes from Livers from Non-Heart-Beating Donors for Cell Transplantation ,
Liver Transpl
 12 , 713 , 2006
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
EBSS
Liver
Human
Human
Hussain, S. , Strom, S. , Kirby, M. , Burns, S. , Langemeijer, S. , Ueda, T. , Hsieh, M. and Tisdale, J.
Side Population Cells Derived from Adult Human Liver Generate Hepatocyte-Like Cells In Vitro. ,
Dig Dis Sci
 50 , 1755 , 2005
Cells
Hepatic side population
Enzymes
Collagenase : 0.02-0.05%
Medium
HBSS
Liver
Human
Human, adult
Cho JJ, Joseph B, Sappal BS, Giri RK, Wang R, Ludlow JW, Furth ME, Susick R. and Gupta S.
  Analysis of the functional integrity of cryopreserved human liver cells including xenografting in immunodeficient mice to address suitability for clinical applications ,
Liver Int
 24 , 361 , 2004
Cells
Hepatocytes
Enzymes
Collagenase : 0.6%
Medium
RPMI 1640
Liver
Human
Human, fetal
Malhi, H. , Irani, A. , Gagandeep, S. and Gupta, S.
Isolation of Human Progenitor Liver Epithelial Cells with Extensive Replication Capacity and Differentiation into Mature Hepatocytes. ,
J Cell Sci
 115 , 2679 , 2002
Cells
Epithelial progenitor
Enzymes
Collagenase : 0.03%
Medium
DMEM
Liver
Human
Human
Duanmu Z. , Locke D. , Smigelski J. , Dahn M. , Falany C. , Kocarek T. and Runge-Morris M.
Effects of Dexamethasone on Aryl (SULT1A1)- and hydroxysteroid (SULT2A1)-Sulfotransferase Gene Expression in Primary Cultured Human Hepatocytes ,
Drug Metab Dispos
 30(9) , 997 , 2002
Cells
Hepatocytes
Enzymes
Medium
Williams E
Liver
Human
Human
(also rat)
Dandri, M. , Burda, M. , Torok, E. , Pollok, J. , Iwanska, A. , Sommer, G. and et al.
Repopulation of Mouse Liver with Human Hepatocytes and In Vivo Infection with Hepatitis B Virus ,
Hepatol
 33 , 981 , 2001
Cells
Hepatocytes
Enzymes
Medium
Leffert's buffer
Liver
Human
Human
Donato M, Viitala P, Rodriguez-Antona C, Lindfors A, Castell J, Raunio H, Gomez-Lechon M, Pelkonen O.
CYP2A5/CYP2A6 expression in mouse and human hepatocytes treated with various in vivo inducers ,
Drug Metab Dispos
 28 , 1321-6 , 2000
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
Williams E
Liver
Human
Human
Parzefall, W. , Erber, E. , Sedivy, R. and Schulte-Hermann, R.
Testing for Induction of DNA Synthesis in Human Hepatocyte Primary Cultures by Rat Liver Tumor Promoters ,
Cancer Res
 51 , 1143 , 1991
Cells
Hepatocytes
Enzymes
Medium
Williams E
Liver
Human
Human
Gomez-Lechon, M. , Lopez, P. , Donato, T. , Montoya, A. , Larrauri, A. , Gimenez, P. , Trullenque, R. , Fabra, R. and Castell, J.
Culture of Human Hepatocytes From Small Surgical Liver Biopsies: Biochemical Characterization And Comparison With in vivo ,
In Vitro Cell Dev Biol
 26 , 67 , 1990
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
HEPES buffer
(see reference)
Liver
Human
Human, male, 17-40 yrs.
Begue, J. , Baffet, G. , Campion, J. and Guillouzo, A.
Differential Response of Primary Cultures of Human and Rat Hepatocytes to Aflatoxin B1-Induced Cytotoxicity and Protection by the Hepatoprotective agent(+)-Cyanidanol-3 ,
Biol Cell
 63 , 327 , 1988
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
HEPES buffer
Liver
Human
Human, 4 male kidney donors, age between 17 and 31
(also adult rats, male, SD, 180-200 g)
LeBot, M. , Begue, J. , Kernaleguen, D. , Robert, J. , Ratanasavanh, D. , Airiau, J. , Riche, C. and Guillouzo, A.
Different Cytotoxicity and Metabolism of Doxorubicin, Daunorubicin, Epirubicin, Esorubicin, and Idarubicin in Cultured Human and Rat Hepatocytes ,
Biochem Pharmacol
 37 (20) , 3877 , 1988
Cells
Hepatocytes
Enzymes
Collagenase : 0.05% & 0.025%
Medium
HEPES
(see reference)
Liver
Human
Human, 3 human kidney donors, age 20, 23, 25
Gugen-Guillouzo, C. , Campion, J. , Brissot, P. , Glaise, D. , Launois, B. , Bourel, M. and Guillouzo, A.
High Yield Preparation of Isolated Human Adult Hepatocytes by Enzymatic Perfusion of the Liver ,
Cell Biol Int Rep
 6 (6) , 625 , 1982
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
HEPES
Liver
Human
Human, 51-75 years old
(also rat, guinea-pig, rabbit, dog)
Reese, J. and Byard, J.
Isolation And Culture of Adult Hepatocytes from Liver Biopsies ,
In Vitro
 17 , 935 , 1981
Cells
Hepatocytes
Enzymes
Collagenase : 90 u/ml
Medium
CF EGTA perfusate
Liver
Monkey
Monkey, Macaca mulatta, 3-5.5 kg
Weber, A. and Groyer-Picard, M.and Dagher, I.
Hepatocyte Transplantation Techniques: Large Animal Models. ,
Methods Mol Biol
 481 , 83 , 2009
Cells
Hepatocytes
Enzymes
Collagenase Type 1 : 129 u/ml
Medium
DMEM/F12
Liver
Mouse

Mouse

Bahrami, E. , Geiger, T. , Steixner-Kumar, A. , Santacruz, D. , Viollet, C. and Dick, A.
An Optimized Protocol for Isolation of Hepatic Leukocytes Retrieved from Murine and NASH Liver Biopsies. ,
Star Protocols
 4 , 102597 , 2023
Cells
Leukocytes
Enzymes
Collagenase Type 4 : 100 u/ml
Medium
RPMI-1640
Liver
Mouse

7-10 week

Plata-Gómez, A. , Crespo, M. , Arregui, C. , Prado-Rivas, L. , Sabio, G. and Efeyan, A.
Protocol for the Assessment of mTOR Activity in Mouse Primary Hepatocytes. , STAR Protoc Vol. 2 , 100918 , 2021
Cells
Hepatocytes
Enzymes
Medium
Williams E
Liver
Mouse

Mouse

Jung, Y. , Zhao, M. and Svensson, K.
Isolation, Culture, and Functional Analysis of Hepatocytes from Mice with Fatty Liver Disease. ,
Star Protocols
 1 , 100222 , 2020
Cells
Hepatocytes
Enzymes
Medium
HBSS
Liver
Mouse
Mouse
Cabral, F. , Miller, C. , Kudrna, K. , Hass, B. , Daubendiek, J. , Kellar, B. and Harris, E.
Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion. ,
J Vis Exp
 , 2018
Cells
Hepatocytes
Enzymes
Medium
DMEM
Liver
Mouse
Mouse
Bigorgne, A. , John, B. , Ebrahimkhani, M. , Shimizu-Albergine, M. , Campbell, J. and Crispe, I.
TLR4-Dependent Secretion by Hepatic Stellate Cells of the Neutrophil-Chemoattractant CXCL1 Mediates Liver Response to Gut Microbiota. ,
PLoS ONE
 11 , e0151063 , 2016
Cells
Hepatic
Enzymes
Medium
HBSS
Liver
Mouse
Mouse, 10-13 week
Sewing, S. , Boess, F. , Moisan, A. , Bertinetti-Lapatki, C. , Minz, T. , Hedtjaern, M. , Tessier, Y. , Schuler, F. , Singer, T. and Roth, A.
Establishment of a Predictive In Vitro Assay for Assessment of the Hepatotoxic Potential of Oligonucleotide Drugs. ,
PLoS ONE
 11 , e0159431 , 2016
Cells
Hepatocytes
Enzymes
Collagenase Type 2 : 0.2 u/ml
Medium
William's Medium E
Liver
Mouse
Mouse
Weerasinghe, S. , Park, M. , Portney, D. and Omary, M.
Mouse Genetic Background Contributes to Hepatocyte Susceptibility to Fas-mediated Apoptosis. ,
Mol Biol Cell
 27 , 3005-3012 , 2016
Cells
Hepatocytes
Enzymes
Collagenase Type 2 : 150 u/ml
Medium
PBS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.