Bone | Worthington Biochemical

Species	Cells	Enzyme(s)	Medium	Reference
Bovine Bovine	Chondrocytes	Collagenase Type 2 : 0.4%	DMEM	Buschmann, M. , Gluzband, Y. , Grodzinsky, A. and Hunziker, E. Mechanical Compression Modulates Matrix Biosynthesis in Chondrocyte/Agarose Culture , J Cell Sci 108 , 1497 , 1995
Chicken Chick, Peterson/Arbor Acre, male, 4 weeks old (Gallus domesticus)	Chondrocytes	Trypsin : 0.25%	Ham's F12	Rosselot, G. , Reginato, A.M. and Leach, R.M. Development of a Serum-Free System to Study the Effect of Growth Hormone and Insulinlike Growth Factor-1 on Cultured Postembryonic Growth Plate Chondrocytes , In Vitro Cell Dev Biol 28A , 235 , 1992
Chicken Chick, embryo	Vertebrae chondroblasts	Trypsin : 0.25%	Simm's, CMF	Schiltz, J.R. , Mayne R. and Holtzer, H. The Synthesis of Collagen and Glycosaminoglycans by Dedifferentiated Chondroblasts in Culture , Differentiation 1 , 97 , 1973
Chicken Chick, day old	Osteoblasts	Trypsin : 0.03%	DMEM	Gay, C. , Lloyd, Q. and Gilman, V. Characteristics and Culture of Osteoblasts Derived From Avian Long Bone , In Vitro Cell Dev Biol 30A , 379 , 1994
Human Human, 22-73 year	Osteoblasts	Trypsin : 0.5%	Basal Medium	Kneser U, Voogd A, Ohnolz J, Buettner O, Stangenberg L, Zhang YH, Stark GB, Schaefer DJ. Fibrin gel-immobilized primary osteoblasts in calcium phosphate bone cement: in vivo evaluation with regard to application as injectable biological bone substitute , Cells Tissues Organs 179 , 158-69 , 2005
Human Human	Osteoblasts	Collagenase Type 2 : 0.2% Trypsin : 0.25%-1.0%	DMEM	Taylor, S. , Shah, M. and Orriss, I. Generation of Rodent and Human Osteoblasts. , Bonekey Rep 3 , 585 , 2014
Human Human	Osteoblasts	Collagenase Type 3 : 0.2%	MEM	Kode, A.,Manavalan1, J.,Mosialou1, I. , Bhagat G. and Rathinam, C, Luo, N et al. Leukemogenesis Induced by an Activating β-catenin Mutation in Osteoblasts , Nature 506 , 240 , 2014
Human Human	Bone-cartilage-stromal	Collagenase Type 2 : 0.75%	HBSS	Agarwal, S. , Loder, S. , Sorkin, M. , Li, S. , Shrestha, S. , Zhao, B. , Mishina, Y. , James, A. and Levi, B. Analysis of Bone-Cartilage-Stromal Progenitor Populations in Trauma Induced and Genetic Models of Heterotopic Ossification. , Stem Cells 34 , 1692-701 , 2016
Human Human	Valve interstitial and endothelial HUVEC Osteoblast	Collagenase Type 4 : 0.1% Collagenase Type 2 : 0.1-0.2%	ECM	Klauzen, P. , Semenova, D. , Kostina, D. , Uspenskiy, V. and Malashicheva, A. Purinergic Signaling in Pathologic Osteogenic Differentiation of Aortic Valve Interstitial Cells from Patients with Aortic Valve Calcification. , Biomedicines Vol. 11 , 2023
Human Human	Osteoblasts	Collagenase Type 4 : 250 u/ml	DMEM	Fedarko, N.S. , Termine, J.D. , Young, M.F. and Robey, P.G. Temporal Regulation of Hyaluronan and Proteoglycan Metabolism by Human Bone Cells in Vitro , J Biol Chem 265 , 12200 , 1990
Human Human	Osteoblasts	Trypsin : 0.1%	DMEM	Meikle, M. , Boyd, S. , Hembry, R. , Compston, J. , Croucher, P. and Reynolds, J. Human Osteoblasts in Culture Synthesize Collagenase and Other Matrix Metalloproteinases in Response to Osteotropic Hormones and Cytokines , J Cell Sci 103 , 1093 , 1992
Human Human, 60+ year	Bone Cells, Osteoblasts	Collagenase Type 2 : 200-250 u/ml	DMEM	Chen X. , Qian H. , Neff L. , Satomura K. and Horowitz M. Thy-1 antigen Expression by Cells in the Osteoblast Lineage , J Bone Miner Res 14 , 362 , 1999
Mouse Mouse	Bone-cartilage-stromal	Collagenase Type 2 : 0.75%	HBSS	Agarwal, S. , Loder, S. , Sorkin, M. , Li, S. , Shrestha, S. , Zhao, B. , Mishina, Y. , James, A. and Levi, B. Analysis of Bone-Cartilage-Stromal Progenitor Populations in Trauma Induced and Genetic Models of Heterotopic Ossification. , Stem Cells 34 , 1692-701 , 2016
Mouse Mouse	Osteoblast	Collagenase Type 1 : 0.3%	HBSS	Wang, W. , Majihail, G. , Lui, C. and Zhou, L. Osteoblast Sorting and Intracellular Staining of CXCL12. , Bio Protoc 8 , 2018
Mouse Mouse	Bone and bone marrow stroma	Stemxyme 1 : 0.1% Neutral Protease : 0.1%	Media 199	Baryawno, N. , Przybylski, D. , Kowalczyk, M. , Kfoury, Y. , Severe, N. , Gustafsson, K. , Kokkaliaris, K. , Mercier, F. , Tabaka, M. , Hofree, M. , Dionne, D. , Papazian, A. , Lee, D. , Ashenberg, O. and Subramanian, A. A Cellular Taxonomy of the Bone Marrow Stroma in Homeostasis and Leukemia. , Cell 177 , 1915-1932.e16 , 2019
Mouse Mouse, Swiss-Webster	Neonatal bone	Collagenase Type 2 : 0.20%	Tris-buffered saline	Chen,T. and Feldman,D. Regulation of 1,25-Dihydroxyvitamin D3 Receptors in Cultured Mouse Bone Cells , J Biol Chem 256 , 5561 , 1981
Mouse Mouse, BALB/c	Osteoblast-like Cells Stromal Cell Lines Hematopoietic Blast Cells	Trypsin : 0.1%	Eagle's MEM	Takanashi, H. , Matsuishi, T. and Yoshizato, K. Establishment and Characterization of Stromal Cell Lines That Support Differentiation of Murine Hematopoietic Blast Cells into Osteoblast-like Cells , In Vitro Cell Dev Biol 30A , 384 , 1994
Mouse Mouse, male, 6-8 week old	Osteoclasts	Collagenase Type 3 : 0.1%	DMEM	Sakai, E. , Miyamoto, H. , Okamoto, K. , Kato, Y. , Yamamoto, K. and Sakai, H. Characterization of Phagosomal Subpopulations Along Endocytic Routes in Osteoclasts and Macrophages , J. Biochem. 130 , 823 , 2001
Mouse Mouse, 3-5 day, 6-8 week	Bone Cells, Osteoblasts	Collagenase Type 2 : 200-250 u/ml	DMEM	Chen X. , Qian H. , Neff L. , Satomura K. and Horowitz M. Thy-1 antigen Expression by Cells in the Osteoblast Lineage , J Bone Miner Res 14 , 362 , 1999
Mouse Mouse	Endosteal cells	Collagenase Type 1 : 0.3%	DMEM	Nakamura, Y. , Arai, F. , Iwasaki, H. , Hosokawa, K. , Kobayashi, I. , Gomei, Y. , Matsumoto, Y. , Yoshihara, H. and Suda, T. Isolation and Characterization of Endosteal Niche Cell Populations that Regulate Hematopoietic Stem Cells. , Blood 116 , 1422 , 2010
Mouse Mouse, 4-22 month	Osteocytes	Collagenase Type 1 : 300 u/ml	MEM	Stern, A. , Stern, M. , Van Dyke, M. , Jahn, K. , Prideaux, M. and Bonewald, L. Isolation and Culture of Primary Osteocytes from the Long Bones of Skeletally Mature and Aged Mice. , Biotechniques 52 , 361 , 2012
Mouse Mouse	Osteoblasts	Collagenase Type 1 : 1.0% Neutral Protease : 1.6%	MEM	Lee, D. , Choung, H. , Kim, H. , Gronostajski, R. , Yang, Y. , Ryoo, H. , Lee, Z. , Kim, H. , Cho, E. and Park, J. NFI-C Regulates Osteoblast Differentiation Via Control of Osterix Expression. , Stem Cells 32 , 2467-79 , 2014
Mouse Mouse	Bone marrow	Collagenase : 0.2%	DMEM	Morikawa, S. , Mabuchi, Y. , Kubota, Y. , Nagai, Y. , Niibe, K. , Hiratsu, E. , Suzuki, S. , Miyauchi-Hara, C. , Nagoshi, N. , Sunabori, T. , Shimmura, S. , Miyawaki, A. , Nakagawa, T. , Suda, T. , Okano, H. and Matsuzaki, Y. Prospective Identification, Isolation, and Systemic Transplantation of Multipotent Mesenchymal Stem Cells in Murine Bone Marrow. , J Exp Med 206 , 2483-96 , 2009
Mouse Mouse	Bone stromal	Collagenase Type 1 : 0.3%	DMEM	Igarashi, H. , Akahoshi, N. , Ohto-Nakanishi, T. , Yasuda, D. and Ishii, S. The Lysophosphatidic Acid Receptor LPA4 Regulates Hematopoiesis-Supporting Activity of Bone Marrow Stromal Cells. , Sci Rep 5 , 11410 , 2015
Mouse Mouse, 2-5 day	Osteoblasts	Collagenase Type 2 : 0.2% Trypsin : 0.25%-1.0%	DMEM	Taylor, S. , Shah, M. and Orriss, I. Generation of Rodent and Human Osteoblasts. , Bonekey Rep 3 , 585 , 2014
Mouse Mouse	Bone and bone marrow stromal	Stemxyme 1 : 0.1% Neutral Protease : 0.1%	PBS	Mohrin, D. , Koukos, G. , Alabdulaaly, L. , Paw, J. , Hake, K. , Kolumam, G. , Bouxsein, M. , Baron, R. , Kutskova, Y. , Freund, A. , Liu, J. , Zavala-Solorio, J. , Bhargava, S. , Maxwell, Trumble, J. , Brito, A. , Hu, D. and Brooks, D. Inhibition of Longevity Regulator PAPP-A Modulates Tissue Homeostasis via Restraint of Mesenchymal Stromal Cells. , Aging Cell Vol. 20 , e13313 , 2021
Mouse Mouse	Osteoblasts	Collagenase Type 3 : 0.2%	MEM	Kode, A.,Manavalan1, J.,Mosialou1, I. , Bhagat G. and Rathinam, C, Luo, N et al. Leukemogenesis Induced by an Activating β-catenin Mutation in Osteoblasts , Nature 506 , 240 , 2014
Rat Rat, newborn	Osteoblastlike cells	Collagenase Type 2 : 0.3%	MEM	Ernst, M. and Froesch, E. Osteoblastlike Cells in a Serum-Free Methylcellulose Medium Form Colonies: Effects of Insulin and Insulinlike Growth Factor I , Calcif Tissue Int 40 , 27 , 1987
Rat Rat, fetal, 21 days of gestation	Calvaria	Collagenase : 0.2%	MEM	Owen, T. , Aronow, M. , Shalhoub, V. , Barone, L. , Wilming, L. , Tassinari, M. , Kennedy, M. , Pockwinse, S. , Lian, J. and Stein, G. Progressive Development of the Rat Oseoblast Phenotype In Vitro: Reciprocal Relationships in Expression of Genes Associated with Osteoblast Proliferation and Differentiation During Formation of the Bone Extracellular Matrix , J Cell Physiol 143 , 420 , 1990
Rat Rat, fetus, 17-21 day	Calvaria	Collagenase : 0.01%-0.6%	Tris-buffered saline	Peck, W. , Birge, S. and Fedak, S. Bone Cells: Biochemical and Biological Studies After Enzymatic Isolation , Science 146 , 1476 , 1964
Rat Rat, 2-3 day	Osteoblasts	Collagenase Type 2 : 0.2% Trypsin : 0.25%-1.0%	DMEM	Taylor, S. , Shah, M. and Orriss, I. Generation of Rodent and Human Osteoblasts. , Bonekey Rep 3 , 585 , 2014
Rat Rat, Wistar, 250g	Proximal femur stem	Collagenase Type 1 : 0.075%	HBSS	Jacobs, F. , Gijsen, H. , Van de Vyver, M. and Ferris, W. Vanadate Impedes Adipogenesis in Mesenchymal Stem Cells Derived from Different Depots within Bone. , Front Endocrinol 7 , 108 , 2016
Rat Rat, 2 day	Osteoblasts	Collagenase Type 2 : 0.1% Trypsin : 0.25%	DMEM	Jeon, J. , Lee, M. and Yang, H. Differentiated Osteoblasts Derived Decellularized Extracellular Matrix to Promote Osteogenic Differentiation. , Biomater Res 22 , 4 , 2018