Spleen

Species	Cells	Enzyme(s)	Medium	Reference
Mouse Mouse, 4-6 week	Dentritic	Collagenase Type 1 : 0.1% Deoxyribonuclease I : 0.001%	RPMI-1640	McLellan, A. , Kapp, M. , Eggert, A. , Linden, C. , Bommhardt, U. , Brocker, E. , Kammerer, U. and Kampgen, E. Anatomic Location and T-cell Stimulatory Functions of Mouse Dendritic Cell Subsets Defined by CD4 and CD8 Expression , Blood 99 , 2084-93 , 2002
Mouse Mouse	Dentritic	Collagenase Type 3 : 0.1% Deoxyribonuclease I : 325 u/ml	RPMI 1640	Schiavoni, F, Mattei, F, Sestili, P, Borghi, P, Venditti, M, Morse, H, Belardelli, F. and Gabrieli, L ICSBP is Essential for the Development of Mouse Type I Interferon-producing Cells and for the Generation and Activation of CD8a+ Dendritic Cells , J Exp Med 196 , 1415 , 2002
Mouse Mouse	Dentritic	Collagenase : 300 u/ml Deoxyribonuclease I : 0.002%	RPMI 1640	Abe, K. , Nguyen, K. , Fine, S. , Mo, J. , Shen, C. , Shenouda, S. , Corr, M. , Jung, S. , Lee, J. , Eckmann, L. and Raz, E. Conventional Dendritic Cells Regulate the Outcome of Colonic Inflammation Independently of T Cells. , Proc Natl Acad Sci U S A 104 , 17022 , 2007
Mouse Mouse, female, 6-8 week	Dendritic	Collagenase Type 4 : 0.05%	RPMI 1640	Abou Fakher, F. , Rachinel, N. , Klimczak, M. , Louis, J. and Doyen, N. TLR9-Dependent Activation of Dendritic Cells by DNA from Leishmania Major Favors Th1 Cell Development and the Resolution of Lesions. , J Immunol 182 , 1386 , 2009
Mouse Mouse	Spleen, bone marrow endothelial	Collagenase Type 4 : 0.3-1.0% Deoxyribonuclease I : 20 u/ml	PBS	Shi, C. , Jia, T. , Mendez-Ferrer, S. , Hohl, T. , Serbina, N. , Lipuma, L. , Leiner, I. , Li, M. , Frenette, P. and Pamer, E. Bone Marrow Mesenchymal Stem and Progenitor Cells Induce Monocyte Emigration in Response to Circulating Toll-Like Receptor Ligands. , Immunity 34 , 590 , 2011
Mouse Mouse	Dendritic	Collagenase Type 1 : 0.5%	HBSS	Flano, E. , Jewell, N. , Durbin, R. and Durbin, J. Methods Used to Study Respiratory Virus Infection. , Curr Protoc Cell Biol Vol. Chapter 26 , Unit 26.3 , 2009
Mouse Mouse	Leukocytes	Collagenase Type 4 : 43 u/ml	RPMI 1640	Siragam, V. , Crow, A. , Brinc, D. , Song, S. , Freedman, J. and Lazarus, A. Intravenous Immunoglobulin Ameliorates ITP via Activating Fc Gamma Receptors on Dendritic Cells. , Nat Med 12 , 688 , 2006
Mouse Mouse	Splenic	Collagenase Type 2 : 0.1%	PBS	Klebanoff, C. , Spencer, S. , Torabi-Parizi, P. , Grainger, J. , Roychoudhuri, R. , Ji, Y. , Sukumar, M. , Muranski, P. , Scott, C. , Hall, J. , Ferreyra, G. , Leonardi, A. and Borman, Z. et al. Retinoic Acid Controls the Homeostasis of Pre-cDC-Derived Splenic and Intestinal Dendritic Cells. , J Exp Med 210 , 1961 , 2013
Mouse Mouse	Splenic	Collagenase Type 2 : 0.1% Deoxyribonuclease I : 0.1%	RPMI	Mueller, S. , Matloubian, M. , Clemens, D, Sharpe, A. , Freeman, G. , Gangappa, S. , Larsen, C. and Ahmed, R. Viral Targeting of Fibroblastic Reticular Cells Contributes to Immunosuppression and Persistence During Chronic Infection. , Proc Natl Acad Sci U S A 104 , 15430 , 2007
Mouse Mouse	Splenocytes	Collagenase Type 2 : 0.16% Deoxyribonuclease I : 0.002%	PBS	Yi, T. and Cyster, J. EBI2-Mediated Bridging Channel Positioning Supports Splenic Dendritic Cell Homeostasis and Particulate Antigen Capture. , Elife 2 , e00757 , 2013
Mouse Mouse	Dentritic cells	Collagenase Type 4 : 0.1% Deoxyribonuclease I : 20 ug/ml	HEPES/RPMI 1640	Stagg, AJ, Burke, F, Hill, S. and Knight, S. Isolation of Mouse Spleen Dendritic Cells. , Methods Mol Med 64 , 9-22 , 2001
Mouse Mouse	Splenic dendritic	Collagenase : 0.1% Deoxyribonuclease I : 0.005%	DMEM	Kang, H. , Liu, M. and Datta, S. Low-dose Peptide Tolerance Therapy of Lupus Generates Plasmacytoid Dendritic Cells That Cause Expansion of Autoantigen-Specific Regulatory T Cells and Contraction of Inflammatory Th17 Cells , J Immunol 178 , 7849-58 , 2007
Mouse Mouse, 4 week	Trophoblast	Collagenase Type 1 : 0.1% Collagenase Type 2 : 0.25%	DMEM	Hou, M. , Han, J. , Li, G. , Kwon, M. , Jiang, J. , Emani, S. , Taglauer, E. , Park, J. , Choi, E. , Vodnala, M. , Fong, Y. , Emani, S. , Rosas, I. , Perrella, M. and Liu, X. Multipotency of Mouse Trophoblast Stem Cells , Stem Cell Res Ther 11 , 55 , 2020
Mouse Mouse	Speen	Collagenase Type 5 : 5% Hyaluronidase : 2.5% Elastase : 1.25%	HBSS	Villarreal, E. , Hewgley, W. , Lang, W. , Morton, C. , Mao, S. , Wu, J. and Sandoval, J. In-bag Enzymatic Splenic Digestion: A Novel Alternative to Manual Morcellation? , J Surg Res 218 , 209-216 , 2017
Mouse Mouse	Splenic stromal	Collagenase Type 3 : 100-400 u/ml	HBSS	Benedict Chris A, De Trez Carl, Schneider Kirsten, Ha Sukwon, Patterson Ginelle, Ware Carl F. Specific remodeling of splenic architecture by cytomegalovirus , PLoS Pathog 2 , e16 , 2006
Mouse Mouse	T cells	Collagenase Type 1 : 0.1% Collagenase Type 2 : 0.2% Elastase : 2 u/ml Soybean Trypsin Inhibitor : 0.025% Hyaluronidase : 50 u/ml	RPMI	Comeau, K. , Caillon, A. , Paradis, P. and Schiffrin, E. Determination of Interleukin-17A and Interferon-γ Production in γδ, CD4, and CD8 T Cells Isolated from Murine Lymphoid Organs, Perivascular Adipose Tissue, Kidney, and Lung. , Bio Protoc 13 , e4679 , 2023
Mouse Mouse	Dentritic	Collagenase : 100 u/ml	HBSS	Brasel K, De Smedt T, Smith JL, Maliszewski CR. Generation of murine dendritic cells from flt3-ligand-supplemented bone marrow cultures , Blood 96 , 3029-39 , 2000