Reproductive Tissue Tables

Species Cells Enzyme(s) Medium Reference
Bovine
Calf, mid to late gestational
Fibroblasts Medium 199
Coplen, D. , Howard, P. , Duckett, J. , Snyder, H. and Macarak, E.
Bovine
Bovine
Epithelial
Endometrial
DMEM/EBSS
Munson, L. , Chandler, S. and Schlafer, D.
Long-Term Culture of Bovine Trophoblastic Cells ,
J Tiss Cul Meth
11 (3)
, 123 , 1988
Bovine
Bovine
(also porcine, human)
Interna & corpus luteum
Endometrium
Ovarian
Uterine
  • Pronase : 0.1%
Moscona's BSS
Marcus, G. , Connor, L. , Domingo, M. , Tsang, B. , Downey, B. and Ainsworth, L.
Bovine
Bovine
Corpus leuteal cells M-199
Levy N, Gordin M, Mamluk R, Yanagisawa M, Smith M F, Hampton J H, Meidan R.
Bovine
Bovine, female
Leuteal Ham's F-12
Canine
Canine
Amniotic membrane MSC LG-DMEM
Chicken
Chicken, Gallus Domesticus,20-30 week
Primary follicles Dulbecco's phosphate buffered saline
Du Meihong, Han Haitang, Jiang Bin, Zhao Chen, Qian Changsong, Shen Haiyan, Xu Yan, Li Zandong.
Chicken

Chicken, male, 1 day

DMEM
Huang, H. , Wang, M. , Hou, L. , Lin, X. , Pan, S. , Zheng, P. and Zhao, Q.
Fish
Sebastes Schlegel
Ovarian L-15
Frog
Xenopus laevis, mature female
Oocytes CF Medium
Moriarty, T. , Gillo, B. , Carty, D. , Premont, R. , Landau, E. and Iyengar, R.
Frog
Xenopus laevis, female
Oocytes Barth's solution, CF
Karkanias, N. and Papke, R.
Frog
Xenopus laevis, female
Oocytes CF Medium
Frog
Xenopus
Oocytes (see reference)
Tian, J. , Kim, S. , Heilig, E. and Ruderman, J.
Frog
Xenopus laevis, female
Oocytes see reference
Pannaccione Anna, Castaldo Pasqualina, Ficker Eckhard, Annunziato Lucio, Taglialatela Maurizio.
Frog
Xenopus laevis, female
Oocytes see reference
Frog
Xenopus laevis, female
Oocytes Barth's solution, CF
Cohen, S. , Au, S. and Pante, N.
Frog
Xenopus laevis
Oocytes Barth's solution
Chatzidaki, A. , D'Oyley, J. , Gill-Thind, J. , Sheppard, T. and Millar, N.
Frog
Xenopus Laevis, female
Oocytes Barth's solution
Nicoll, G, Jawad, A. , Weymouth, R, Zhang, H. and Beg, A.
Frog
Xenopus
Oocytes see reference
O'Connell, D. , Mruk, K. , Rocheleau, J. and Kobertz, W.
Hamster
Hamster, Chinese
Ovary Dialyzed fetal calf serum, 10% and 0.5M Methotrexate
Human
Human
Uterine epithelial HBSS
Human
Human, female, 27 years
Smooth muscle, uterine EBSS
Rifas, L. , Fant, J. , Makman, M. and Seifter, S.
Human
Human
Endometrial endothelial cells McCoys medium
Nikitenko LL, MacKenzie IZ,Rees MC, Bicknell R.
Human
Human
Mesenchymal stem CMF-DPBS
Human
Human
(also porcine, bovine)
Interna & corpus luteum
Endometrium
Ovarian
Uterine
  • Pronase : 0.1%
Moscona's BSS
Marcus, G. , Connor, L. , Domingo, M. , Tsang, B. , Downey, B. and Ainsworth, L.
Human
Human, female, 34-51 yr
Endometrial epithelial and stromal cells DMEM/F-12
Chan Rachel W S, Schwab Kjiana E, Gargett Caroline E.
Human
Human, females, 25-45 yr
Luteal cells PBS
Friden BE, Hagstrom H, Lindblom B, Sjoblom P, Wallin A, Brannstrom M, Hahlin M.
Human
Human
Extravillanous trophoblasts and decidual stromal HBSS RPMI
Spessotto, P. , Bulla, R. , Danussi, C. , Radillo, O. , Cervi, M. , Monami, G. , Bossi, F. , Tedesco, F. , Doliana, R. and Colombatti, A.
Human
Human
Trophoblasts, placental EBSS, CMF
Branchaud, C.L. , Goodyer, C.G. , Guyda, H.J. and Lefebvre, Y.
Human
Human, female, 20-40 year
Microvascular endothelial cells PBS
Human
Human
Mesenchymal stem DMEM/F12
Shalini, V. , Pratheep, S. , Muhammad, A. , Sharmili, V. , Elizabeth, G. and Rajesh, R.
Human
Human
Trophoblasts, placental PBS
Jie, Z. , Fey, S. , Hager, H. , Hollsberg, P. , Ebbesen, P. and Larsen, P.
Human
Human, female
Endometrial stromal cells DMEM/F-12
Human
Human
Endothelial colony forming HBSS
Patel, J. , Seppanen, E. , Chong, M. , Yeo, J. , Teo, E. , Chan, J. , Fisk, N. and Khosrotehrani, K.
Human
Human
Epithelial, fallopian tube Medium 199
Takeuchi, K. , Maruyama, I. , Yamamoto, S. , Oki, T. and Nagata, Y.
Human
Human
Endothelial placental DMEM
Human
Human
Endometrial epithelial and stromal fibroblasts DMEM
Human
Human
Amnion epithelial and fibroblast PBS
Human
Human, female
Endometrial epithelial cells DMEM/F-12
Zhang J, Lathbury LJ, Salamonsen LA.
Human
Human
Endometrial stromal DMEM/F-12
Human
Human, female, 27-49 years
Epithelial
Ovary
HBSS, CMF
Auersperg, N. , Siemens, C.H. and Myrdal, S.E.
Human
Human
Theca cells PBS
Runesson E, Ivarsson K, Janson P, Brannstrom M.
Human
Human, infant and neonate
Epithelial
Prostate
HBSS
Lechner, J. , Babcock, M. , Marnell, M. , Narayan, K. and Kaighn, M.
Normal Human Prostate Epithelial Cell Cultures ,
Methods Cell Biol
21
, 195 , 1980
Human
Human, female
Mesothelial Eagle's MEM
Witz Craig A, Allsup Karen T, Montoya-Rodriguez Iris A, Vaughn Shelby L, Centonze Victoria E, Schenken Robert S.
Human
Human
Placental PSS
Bradbury. , Sunn, K. , Crossley, M. , Bai, M. , Brown, E. , Delbridge, D. and Conigrave, A.
Human
Human
Chorionic villi HBSS
Human
Human
Placental DMEM
Human
Human
Stromal endometrial DMEM/Ham's F12
Zhang J, Nie G, Jian W, Woolley DE, Salamonsen LA.
Human
Human
Epithelial
Stromal
DMEM
Kirk, D. , King, R. , Heyes, J. , Peachey, L. , Hirsch, P. and Taylor, W.
Normal Human Edometrium in Cell Culture ,
In Vitro
14 (8)
, 651 , 1978
Human
Human
Sertoli cells DMEM/F-12
Teng Yan, Xue Wu-jun, Ding Xiao-ming, Feng Xin-shun, Xiang He-li, Jiang Ya-zhuo, Tian Pu-xun.
Human
Human
Epithelial
Stromal
See reference
Siegfried, J. , Nelson, K. , Martin, J. and Kaufman, D.
Human
Human, female, 25-41 year
Follicles EBSS
Hovatta O, Wright C, Krausz T, Hardy K, Winston RM.
Human
Human, embryo
Endothelial
Hematopoietic
Stromal
DMEM
Oberlin, E. , Tavian, M. , Blazsek, I. and Peault, B.
Human
Human
Corpus luteum cells PBS
Friden BE, Runesson E, Hahlin M, Brannstrom M.
Human
Human
Stem, embryonic DMEM
Zhang, S. , Wernig, M. , Duncan, I. , Brustle, O. and Thomson, J.
Human
Human, female
Decidual DMEM/F12
Lockwood, C. , Arcuri, F. , Toti, P. , Felice, C. , Krikun, G. , Guller, S. , Buchwalder, L. and Schatz, F.
Human
Human
Uterine epithelial cells HBSS
Human
Human, female
Endometrial RPMI 1640
Rinehart, C. , Lyn-Cook, B. and Kaufman, D.
Gland formation from human endometrial epithelial cells in vitro ,
In Vitro Cell Dev Biol
24 (10)
, 1037 , 1988
Human
Human, female
Uterine epithelial HBSS
Human
Human
Chorionic, placental HBSS
Egan, D. , Grzegorczyk, V. , Tricarico, K. , Rueter, A.H. , Olleman, W. and Marcotte, P.
Human
Human, 20-40 yr
Endometrium epithelial and stromal cells HBSS
Arnold, J. , Kaufman, D. , Seppala, M. and Lessey, B.
Insect
Drosophila
Ovarian Grace's
Ma, X. , Wang, S. , Do, T. , Song, X. , Inaba, M. , Nishimoto, Y. , Liu, L. , Gao, Y. , Mao, Y. , Li, H. , McDowell, W. , Park, J. , Malanowski, K. , Peak, A. , Perera, A. and Li, H. et al.
Insect
Drosophila, embryonic
Cardiac differentiating Schneider
Salmand, P. Iche-Torres, M. and Perrin, L.
Monkey
Rhesus macaque
Follicle HEPES
Xu, J, Lawson, MS, Yeoman, RR, Pau, KY, Barrett, SL, Zelinski, MB. and Stouffer, RL
Mouse
Mouse, BALB/cCrg, 40 days old
Epithelial Medium 199
Cooke, P. , Uchima, F. , Fujii, D. , Bern, H. and Cunha, G.
Mouse
Mouse
Zona pellucid free eggs THY-HEPES
Yamatoya, K. , Ito, C. , Araki, M. , Furuse, R. and Toshimori, K.
Mouse
Mouse, male, 3.5 months old
Leydig Medium E 199
Faldikova, L. , Diblikova, I. , Canderle, J. , Zraly, Z. , Veznik, Z. and Sulcova, A.
Mouse
Mouse, 4 week
Trophoblast DMEM
Hou, M. , Han, J. , Li, G. , Kwon, M. , Jiang, J. , Emani, S. , Taglauer, E. , Park, J. , Choi, E. , Vodnala, M. , Fong, Y. , Emani, S. , Rosas, I. , Perrella, M. and Liu, X.
Multipotency of Mouse Trophoblast Stem Cells ,
Stem Cell Res Ther
11
, 55 , 2020
Mouse
Mouse, male, 12-14-week-old
Seminiferous tubules DMEM
Lin, Q. , Sirotkin, A. and Skoultchi, A.
Mouse
Mouse, adult
Testes MEM
Lee, A, Rusch, J. , Lima, A. , Usmani, A. , Huang, N. , Lepamets, M. , Vigh-Conrad, K. , Worthington, R. and et al.
Mouse
Mouse, 6 day
Sertoli DMEM
Nalbandian Angele, Dettin Luis, Dym Martin, Ravindranath Neelakanta.
Mouse
Mouse, adult, neonatal and fetal
Testicular cells DMEM/F-12
O'Shaughnessy PJ, Fleming LM, Jackson G, Hochgeschwender U, Reed P, Baker PJ.
Mouse
Mouse, 6-12 day
Oocyte-granulosa Waymouth
Eppig JJ, O'Brien MJ.
Mouse
Mouse, female, 21 day, 10-12 g
vaginal epithelial DMEM
Mouse
Mouse
Germ cells HBSS
Breault, D. , Min, I. , Carlone, D. , Farilla, L. , Ambruzs, D. , Henderson, D. , Algra, S. , Montgomery, R. , Wagers, A. and Hole, N.
Mouse
Mouse (C57B/T), fetus, 16-17 days old
Prostate HBSS/ DMEM
Thompson, T.C. , Southgate, J. , Kitchener, G. and Land, H.
Mouse
Mouse, male
Testis, meiotic Gey's BSS
Getun, I. , Torres, B. and Bois, P.
Mouse
Mouse, male, 10-13 wk
Leydig
Testis
Medium 199 w/ BSA
Mouse
Mouse
Ovarian HBSS
White, Y. , Woods, D. , Takai, Y. , Ishihara, O. , Seki, H. and Tilly, J.
Mouse
Mouse, CF1, female
Cumulus, one-cell embryos PBS, CMF
Mouse
Mouse, adult
Spermatogonial stem DMEM
Mouse
Mouse (BALB/cCRGL), male, 2-3 months
Epithelial, prostate gland Medium 199
Turner, T. , Bern, H. , Young,P. and Cunha, G.
Mouse
Mouse
Uterine stomal DMEM/F12
Chen, L. , Belton, R. and Nowak, R.
Mouse
Mouse, female,6-8 weeks
Uterine HBSS
Ghosh, D. , Danielson, K. , Alston, J. and Heyner, S.
Mouse
Mouse, female
Lymphocytes RPMI
Mouse
Mouse (BALB/cCrgl), female, 50 - 60 days
Epithelial, vagina HBSS
Iguchi, T. , Uchima, F.A. , Ostrander, P. and Bern, H.
Mouse
Mouse, embryonic
Hematopoietic stem cells PBS
Gekas, C. , Rhodes, K. and Mikkola, H.
Mouse
Mouse, outbred, CD-1, 21-23 days old
Epithelial Medium 199
Tomooka, Y. , DiAugustine, R. and McLachlan, J.
Mouse
Mouse
Vaginal smooth muscle DMEM/F12
Venkataraman, L. , Lenis, A. , Couri, B. , Damaser, M. and Ramarurthi, A
Mouse
Mouse, BALB/c
Epithelial
Mesencymal
DMEM
Bigsby, R. , Cooke, P. and Cunha, G.
Mouse
Mouse, 1-7 day
Testicular HBSS
Kang, H. , Chen, L. , Lichti-Kaiser, K. , Liao, G. , Gerrish, K. , Bortner, C. , Yao, Hu. , Eddy, E. and Jetten, A.
Ovine
Sheep
Epithelial DMEM
Salamonsen, L. , Sum O, W. , Doughton, B. and Findlay, J.
Ovine
Ovine, 3-5 year
Endometrial stromal DMEM/F-12
Letouzey, V. , Tan, K. , Deane, J. , Ulrich, D. , Gurung, S. , Ong, Y. and Gargett, C.
Porcine
Porcine (also bovine, human)
Interna & corpus luteum
Endometrium
Ovarian
Uterine
  • Pronase : 0.1%
Moscona's BSS
Marcus, G. , Connor, L. , Domingo, M. , Tsang, B. , Downey, B. and Ainsworth, L.
Porcine
Porcine, female
Corpus Leuteum Medium 199
Ciereszko, R. , Petroff, B. , Ottobre, A. , Guan, Z. , Stokes, B. and Ottobre, J.
Porcine
Porcine, 3-4 week
Leydig
Testis
Lebovitz L-15 Medium
Mather, J. , Saez, J. and Haour, F.
Porcine
Porcine, male, 8 day
Seminiferous epithelial cells DMEM/F12
Porcine
Porcine, 1-4 day
Testicular DMEM
Park, M. , Park, J. , Kim, M. , Lee, K. , Park, H. , Yun, J. , Choi, J. , Lee, E. and Lee, S.
Rabbit
Rabbit, New Zealand white, adult, female (nonpregnant),
3-4 kg
Myocytes, uterine HBSS-HEPES buffer
Phillippe, M. , Saunders, T. and Bangalore, S.
Rabbit
Rabbit, New Zealand, mature, female, 4-5 Kg
Ovarian surface epithelial and peritoneal mesothelial HBSS, CMF
Rabbit
Rabbit, New Zealand white estrous, female, 4-5 months
Mesothelial and surface epithelial
Ovaries
Medium 199
Nicosia, S. , Johnson, J. and Streibel, E.
Rabbit
Rabbit, New Zealand white, 4-6 month old,
Ovarian
Mesothelial
HBSS
Setrakian, S. , Oliveros-Saunders, B. and Nicosia, S.
Rabbit
Rabbit, mixed breed
Endometrial epithelial DMEM
Mulholland, J. , Winterhager, E. and Beier, H.
Rabbit
Rabbit, New Zealand white, mature, 3-4 Kg
Myometrial HBSS
Rabbit
Rabbit, New Zealand, 3 month
Testicular germ HBSS
Rabbit
Rabbit, New Zealand, 3 month
Testicular germ HBSS
Rat
Rat, Wistar, virgin, female, 250 g
Vaginal epithelial PBS
Conti, C. and Tasat, D.
Rat
Rat, SD, female, 26 days
Luteal Medium 199
Azhar, S. and Reaven, E.
Rat
Rat, SD, male
Leydig Krebs Ringer bicarbonate buffer
Rat
Rat, Han-Wistar, adult, male, 250 g
Testes Medium 199 w/ Hank's salts
Leckie, C. , Welberg, L. and Seckl, J.
Rat
Rat, immature
Luteal, ovaries McCoy's
Rat
Rat, SD, female, immature, 26 day
(also rat, pregnant and pseudopregnant)
Corpus luteum Serum-free medium
(see reference)
Tellieria, C. , Ou, J. , Sugino, N. , Ferguson, S. and Gibori, G.
Rat
Rat, SD, adult,male, 50-70 days
Testicular HEPES
Hsueh, A. , Bambino, T. , Zhuang, L. , Welsh, T. and Ling, N.
Rat
Rat, SD, immature
Ovary McCoy's 5a
Rat
Rat, SD, female, pseudopregnant, 21 day
Luteal, ovaries EBSS
Rat
Rat, SD, adult, male, 8 - 10 week
Seminiferous tubules Krebs-Ringer bicarbonate buffer
(see reference)
Rat
Rat, SD, 4-6 day, male
Sertolli, seminiforous tubules Serum-free medium
Rich, K. , Bardin, C. , Gunsalus, G. and Mather, J.
Rat
Rat, SD, 350-450 g
Leydig cells Medium 199
Sharma RS, Pal PC, Rajalakshmi M.
Isolation and Culture of Leydig Cells from Adult Rats ,
Indian J Clinical Biochem
21
, 27 , 2006
Rat
Rat, Holtzman, female, immature
Uterine DMEM buffered with HEPES
Kassis, J. , Walent, J. and Gorski, J.
Rat
Rat, SD, 10 day
Sertoli cells PBS
Hadley MA, Weeks BS, Kleinman HK, Dym M.
Rat
Rat, SD, female, 21 day
Luteal, ovaries McCoy's
Rat
Rat, SD, 25 day
Ovarian theca-interstitial Medium 199
Duleba AJ, Spaczynski RZ, Olive DL, Behrman HR.
Rat
Rat, SD, male, 120-160 g
Leydig
Adrenal
Krebs Ringer bicarbonate buffer
Ng, T. and Liu, W.
Rat
Rat
Placental mesenchymal DMEM
Rat
Rat, Wistar, adult
Uterine PBS
Pampfer, S. , Vanderheyden, I. , Michiels, B. and DeHertogh, R.
Co-Culture of Two-Cell Rat Embryos on Cell Monolayers ,
In Vitro Cell Dev Biol
26
, 944 , 1990
Rat
Rat, SD, male, 90 days
Sertoli DMEM
Rat
Rat, SD, male
Leydig DMEM
Abayasekara, D. , Kurlak, L. , Band, A. , Sullivan, M. and Cooke, B.
Rat
Rat, neonatal
Uterine HBSS
Branham, W. , Lyn-Cook, B. , Andrews, A. , McDaniel, M. and Sheehan, D.
Rat
Rat, male, 20 day
Sertoli (see reference)
Rat
Rat, SD, male, 10 day
Sertoli DMEM
Rat
Rat, SD, female
Luminal epithelial HBSS
Glasser, S. , Julian, J. , Decker, G. , Tang, J. and Carson, D.

Tissue Tables (references, grouped by tissue type and species)