Brain Tissue Tables

Species Cells Enzyme(s) Medium Reference
Bovine
Bovine
Microvascular endothelial Medium 199
Bovine
Bovine
Brain endothelial cells DMEM
Bovine
Bovine
Endothelial
Brain arteries
Dulbecco's PBS
Goetz, I.. , Warren, J. , Estrada, C. , Roberts, E. and Krause, D.
Bovine
Bovine, adult
Cerebral artery
Endothelial
HBSS
Machi, T. , Kassell, N.F. and Scheld, W.M.
Bovine
Calf
(also lamb)
Oligodendroglia
Neural
(see reference)
Poduslo, S. , Miller, K. and McKhann, G.
Bovine
Bovine
Endothelial MEM
Bovine
Bovine
Endothelial MEM
Bovine
Bovine
Capillary endothelial DMEM
Estrada, C. , Bready, J. , Berliner, J. and Cancilla, P.
Guinea-Pig
Guinea-pig, 200-400 g
Neurons PIPES saline
Kay, A.R. and Wong, R.K.S.
Human
Human
Microvessels DMEM
Gerhart, D. Z. , Broderius, M. A. and Drewes, L. R.
Human
Human, 15-54 years
Microvessels Serum-free modified Lewis medium
Vinters, H.V. , Reave, S. , Costello, P. , Girvin, J.P. and Moore, S.A.
Human
Human
Neuronal Tris-HCl, 50 mM, CaCl2, 2 mM
Human
Human
Microglia DMEM/F12
Human
Human
Tumor see reference
Sauvageot, C. , Weatherbee, J. , Kesari, S. , Winters, S. , Barnes, J. , Dellagatta, J. , Ramakrishna, N. , Stiles, C. , Kung, A. , Kieran, M. and Wen, P.
Human
Human, fetal and mature
Astrocytes and neurons RPMI
Zhang, Y. , Sloan, S. , Clarke, L. , Caneda, C. , Plaza, C. , Blumenthal, P. , Vogel, H. , Steinberg, G. , Edwards, M. , Li, G. , Duncan, J. , Cheshier, S. , Shuer, L. , Chang, E. , Grant, G. , Gephart, M. and Barres, B.
Human
Human
Microglia Hibernate A
Rustenhoven, J. , Park Thomas, I. , Schweder, P. , Scotter, J. , Correia, J. , Smith, A. , Gibbons, H. , Oldfield, R. , Bergin, P. , Mee, E. , Faull, R. , Curtis, M. , Scott, G. and Dragunow, M.
Human
Human, 25-81 yr
Brain tumor HBSS
Volovitz, I, Shapira, N. , Ezer, H. , Gafni, A. , Lustgarten, M. , Alter, T. , Ben-Horin, I. , Barzilai, O. , Shahar, T. , Kanner, A. , Fried, I. , Veshchev, I. , Grossman, R. and Ram, Z,
Human
Brain tumor DMEM/F12
Hussein, D. , Punjaruk, W. , Storer, L. , Shaw, L. , Othman, R. , Ottoman, R. , Peet, A. , Miller, S. , Bandopadhyay, G. , Heath, R. , Kumari, R. , Bowman, K. , Braker, P. , Rahman, R. , Jones, G. , Watson, S. and Lowe, J.
Human
Human
Microglia DMEM
Mizee, M. , Miedema, S. , van der Poel, M. , Adelia , S. , van Strien, M. , Melief, J. , Smolders, J. , Hendrickx, D. , Heutinck, K. , Hamann, J. and Huitinga, I.
Human
Human, adult
Neuronal Neurobasal
Spaethling, J. , Na, Y. , Lee, J. , Ulyanova, A. , Baltuch, G. , Bell, T. , Brem, S. , Chen, H. , Dueck, H. , Fisher, S. , Garcia, M. , Khaladkar, M. , Kung, D. , Lucas, T. , O'Rourke, D. and Stefanik, D.
Human
Human
Amyloid Fibrils 50mM Tris
Scherpelz, K. , Lu, J.,Tycko, R. and Meredith, S.
Insect
Drosophilia
Neurons Modified Dissecting Saline
Hadzic, T. , Park, D. , Abruzzi, K. , Yang, L. , Trigg, J. , Rohs, R. , Rosbash, M. and Taghert, P.
Insect
Drosophila
Neurons Saline
Insect
Drosophila
Neurons DMEM
Sicaeros, B. Campusano, J. and O'Dowd, D.
Insect
Drosophilia
Kenyon Recording saline
Gu, H. and O'Dowd, D.
Monkey
Monkey, rhesus
Brain cells HBSS
Marcondes MC, Burudi EM, Huitron-Resendiz S, Sanchez-Alavez M, Watry D, Zandonatti M, Henriksen SJ, Fox HS.
Mouse
Mouse, 6-12 week
Vascular smooth muscle cells PBS
Chung, W. , Farley, J. , Swenson, A. , Barnard, J. , Hamilton, G. , Chiposi, R. and Drummond, H.
Mouse
Mouse, SWR or CF1, 1-3 months
Papillae, taste receptor
  • Pronase E : 0.15%
Carbonate-Phosphate buffer
(see reference)
Spielman, A. , Mody, I. , Brand, J. , Whitney, G. , MacDonald, J. and Salter, M.
Mouse
Mouse, 1-2 day
Oligodendrocytes, dorsal root ganglia DMEM
Mouse
Mouse, 4-6 day
Neurons HBSS
Mouse
Mouse, 0-30 day
Neural BSS
Shrier, B. , Wilson, S. and Nirenberg, M.
Mouse
Mouse, embryonic and postnatal
Cortical neurons Neurobasal
Mouse
Mouse
Astrocytes DMEM
Lim, D. and Alvarez-Buylla, A.
Mouse
Mouse, neonatal
Neurons Neurobasal
Fasano, C. , Thibault, D. and Trudeau, L.
Culture of Postnatal Mesencephalic Dopamine Neurons on an Astrocyte Monolayer , Current Protocols in Neuroscience Vol. 44 , 3.21.1 , 2008
Mouse
Mouse, 3 day
Microglia Eagle's MEM
Nishioku T, Hashimoto K, Yamashita K, Liou SY, Kagamiishi Y, Maegawa H, Katsube N, Peters C, von Figura K, Saftig P, Katunuma N, Yamamoto K, Nakanishi H.
Mouse
Mouse, adult
Neurons DMEM/F12
Saxena, A. , Wagatsuma, A. , Noro, Y. , Kuji, T. , Asaka-Oba, A. , Watahiki, A. , Gurnot, C. , Fagiolini, M. , Hensch, T. and Carninci, P.
Mouse
Mouse, SD, 8-12 week
Microglia RPMI-1640
O'Donnell SL, Frederick TJ, Krady JK, Vannucci SJ, Wood TL.
Mouse
Mouse
Brain-sequestered leukocytes RPMI
Mouse
Mouse
Cerebellar granule cell precursors EBSS
Miyazawa K, Himi T, Garcia V, Yamagishi H, Sato S. and Ishizaki Y.
Mouse
Mouse
Cerebral pericytes DMEM
Shah. G. , Price, T. , Banks, W. , Morofuji, Y. , Kovac, A. , Ercal, N. , Sorenson, C. , Shin, E. and Sheibani, N.
Mouse
Mouse
Neurosperes DMEM/F12
Klein C, Butt SJ, Machold RP, Johnson JE. and Fishell G.
Mouse
Mouse, P13-15
Glial EBSS
Mouse
Mouse, newborn
Hippocampal cells DMKM
Jun K, Choi G, Yang SG, Choi KY, Kim H, Chan GC, Storm DR, Albert C, Mayr GW, Lee CJ, Shin HS.
Mouse
Mouse
Chloroid plexus PBS
Baruch, K. , Rosenzweig, N. , Kertser, A. , Deczkowska, A. , Sharif, A. , Spinrad, A. , Tsitsou-Kampeli, A. , Sarel, A. , Cahalon, L. and Schwartz, M.
Mouse
Mouse, 2-5 day
Postnal substantia nigra see reference
Smeyne Michelle, Smeyne RichardJ.
Mouse
Mouse, fetal and mature
Astrocytes and neurons RPMI
Zhang, Y. , Sloan, S. , Clarke, L. , Caneda, C. , Plaza, C. , Blumenthal, P. , Vogel, H. , Steinberg, G. , Edwards, M. , Li, G. , Duncan, J. , Cheshier, S. , Shuer, L. , Chang, E. , Grant, G. , Gephart, M. and Barres, B.
Mouse
Mouse
Cortical neurons Neurobasal
Hernandez, F. , Perez, M. , Lucas, J. , Mata, A. , Bhat, R. and Avila, J.
Mouse
Mouse, 3 week
Neural PBS
Luo,Y.,Coskun, V. , Liang, A.,Yu, J. , Cheng, L.,Ge, W. , Shi, Z. , Zhang, K. , Li, C. , Lin, H. , Luo, D. , Wang, J. , Lin, C. , Dai, Z. , Zhu, H. , Zhang, J. , Liu, J. , Liu, H.,deVellis, J. , Horvath, S. , Sun, Y. and Li, S.
Mouse
Mouse, 1 day
Neurons and glia Neurobasal
Martin-Aparicio, E. , Yamamoto, A. , Hernandez, F. , Hen, R. , Avila, J. and Lucas, J.
Mouse
Mouse, P0-P15
Astrocytes DPBS
Foo, L.
Purification of Rat and Mouse Astrocytes by Immunopanning. ,
Cold Spring Harb. Protoc.
2013
, 421-32 , 2013
Mouse
Mouse, 1 day
Neural progenitor see reference
Mouse
Mouse, adult
Astrocytes, microglia, endothelia, oligodendrocytes HBSS
Swartzlander, D. , Propson, N. , Roy, E. , Saito, T. , Saido, T. , Wang, B. and Zheng, H.
Mouse
Mouse, embryonic
Cortical progenitors Serum free medium
Mouse
Mouse, postnatal
Astrocytes HBSS
Sher, F. , Rossler, R. , Brouwer, N. , Balasubramaniyan, V. , Boddeke, E. and Copray, S.
Mouse
Mouse
Granule cell precursors, pre-neoplastic and tumor cells Neurobasal/B27
Oliver, T. , Read, T. , Kessler, J. , Mehmeti, A. , Wells, J. , Huynh, T. , Lin, S. and Wechsler-Reya, R.
Ovine
Lamb
(also calf)
Oligodendroglia
Neural
(see reference)
Poduslo, S. , Miller, K. and McKhann, G.
Ovine
Ovine
Neurons DMEM
Lepore G, Gadau S, Mura A, Zedda M, Farina V.
Ovine
Ovine
Neurons Hibernate
Porcine
Mini pigs, Yucatan (Susscrofa Yucatan), 4-6 months
Microvascular HBSS
Robinson, D.H. , Kang,Y. , Deschner, S.H. and Nielsen, T.B.
Rat
Rat, SD, newborn
Glial MEM, sterile
Pixley, S.K.
Rat
Rat, fetus
Cortical Puck's D1 (see reference)
Swaiman, K. , Neale, E. , Fitzgerald, S. and Nelson, P.
Rat
Rat, SD, 19-21 day
Suprachiasmatic nucleus neurons MEM
Cao, R. , Li, A. , Cho, H. , Lee, B. and Obrietan, K.
Rat
Rat, fetus, 18-20 day
Hippocampal neurons HBSS, CMF
Rat
Rat, SD, 2 days
Pineal DMEM, MEM
Rat
Rat, neonatal
Astrocytes EBSS
Rat
Rat, albino, adult and newborn
Cerebral cortices PBS
Giulian, D. and Baker, T.J.
Rat
Rat, SD, E16
Cortical neurons Neurobasal Medium
Varney MA, Cosford ND, Jachec C, Rao SP, Sacaan A, Lin FF, Bleicher L, Santori EM, Flor PJ, Allgeier H, Gasparini F, Kuhn R, Hess SD, Velicelebi G, Johnson EC.
Rat
Rat, Fisher, 7-21 month
Hippocampal neurons Hibernate A
Chen, N. , Newcomb, J. , Garbuzova-Davis, S. , Davis Sanberg, C. , Sanberg, P. and Willing, A.
Rat
Rat, Wistar, newborn
Germinal matrix HBSS
Goldman, J.E. , Geier, S.S. and Hirano, M.
Rat
Rat, Wistar, 14 day
Visual cortical EBSS
Rat
Rat, neonatal
Dura mater DMEM
Rat
Rat, Wistar-Kyoto, male, 100 - 200 g
Endothelial
Cerebral
HBSS
Diglio, C.A. , Grammas, P. , Filiberto Giacomelli, M.S. and Wiener, J.
Rat
Rat, Wistar, male
Cerebral endothelial MEM
Floris S, Van den Born J, van der Pol SM, Dijkstra CD, De Vries HE.
Rat
Rat, SD
Hippocampal Neurobasal
Rat
Rat, 60-72 hours old
Fibroblasts HEPES buffered DMEM
Acheson, A. , Barker, P. , Alderson, R. , Miller, F. and Murphy, R.
Rat
Rat, wistar, neonatal
Hippocampal cells DMEM
Rat
Rat, SD, 1-2 day
Oligodendrocyte precursor cells HBSS
Rat
Rat, Wistar, adult, 170 g
Endothelial BSS
Phillips, P. , Kumar, P. , Kumar, S. and Waghe, M.
Rat
Rat, wistar, 250-300 g
Cerebral artery smooth muscle cells Physiological Salt Solution
Rat
Rat, P0-P15
Astrocytes DPBS
Foo, L.
Purification of Rat and Mouse Astrocytes by Immunopanning. ,
Cold Spring Harb. Protoc.
2013
, 421-32 , 2013
Rat
Rat, Wistar, pregnant
Glial Eagle's MEM/DMEM
Rat
Rat, SD, Fisher
Hippocampal neurons HibernateA/B27
Brewer, G.J.
Rat
Rat, SD, E18
Neurons HBSS
Folts, C. , Scott-Hewitt, N. , Proschel, C. , Mayer-Proschel, M. and Noble, M.
Rat
Rat, SD, adult
Endothelial Medium 199
Rat
Rat, E18
Hippocampal and cortical neurons Neurobasal media
Naeve GS, Ramakrishnan M, Kramer R, Hevroni D, Citri Y, Theill LE.
Rat
Rat, SD, neonatal
Neurons Neuro medium
Kaiser, O. , Aliuos, P. , Wissel, K. , Lenarz, T. , Werner, D. , Reuter, G. , Kral, A. and Warnecke, A.
Rat
Rat, SD, female
Fetal rat brain DMEM
Rat
Rat, SD, E18
Cortical cells EBSS
Behar, T, Smith, S, Kennedy, R, Mckenzie, J, Maric, I. and Barker, J
Rat
Rat, Wistar, male, 300-500 g
Microvessels
Endothelial
Ringers-HEPES buffer
Williams, S. , Gillis, J. , Matthews, M. , Wagner, R. and Bitensky, M.
Rat
Rat, E18
Hippocampal neurons Hibernate
Rat
Rat, SD, one month old
Capillary endothelium
Pericytes
Medium 199
Bowman, P. , Betz, A. , Ar, D. , Wolinsky, J. , Penney, J. , Shivers, R. and Goldstein, G.
Rat
Rat, E19
Hippocampal neuron MEM
Rat
Rat, newborn
Astrocytes DMEM, HBSS
Rat
Rat, neonatal
Hippocampal neurons EBSS
Richler Esther, Chaumont Severine, Shigetomi Eiji, Sagasti Alvaro, Khakh Baljit S.
Rat
Rat, Holtzmann, 18 day
Hippocampal neurons HBSS
Banker, G. and Cowan, M.
Rat
Rat, Wistar, 1-3 day
Hippocampal neurons DMEM
Velasco Myrian, Garci­a Esperanza, Onetti Carlos G.
Rat
Rat, fetus, 18 day
Hippocampi HBSS
Rat
Rat, SD, 5 day
Hippocampal neurons HBSS
Rat
Rat, SD, fetal
Cerebral cortex
Hypothalmus
HEPES
Peterfreund, R. and Vale, W.
Rat
Rat, SD, 19-20 days pregnant
Neural DMEM
Ahmed, Z. , Walker, P. and Fellows, R.
Rat
Rat, SD, 7 day
Cerebellar granule neurons PBS
Shellfish
Helisoma trivolvis
Neurons
Buccal ganglia
Saline, Sterile

Tissue Tables (references, grouped by tissue type and species)