Brain Tissue Tables
Species | Cells | Enzyme(s) | Medium | Reference |
---|---|---|---|---|
Bovine Bovine |
Capillary endothelial |
|
DMEM |
Choline Uptake by Cerebral Capillary Endothelial Cells in Culture
,
J Neurochem
54
, 1467
, 1990
|
Bovine Bovine |
Microvascular endothelial |
|
Medium 199 | |
Bovine Bovine |
Brain endothelial cells |
|
DMEM | |
Bovine Bovine |
Endothelial Brain arteries |
|
Dulbecco's PBS |
Long-Term Cultivation of Arterial and Capillary Endothelium From Adult Bovine Brain
,
In Vitro Cell Dev Biol
21
, 172
, 1985
|
Bovine Bovine, adult |
Cerebral artery Endothelial |
|
HBSS |
Isolation and Characterization of Endothelial Cells From Bovine Cerebral Arteries
,
In Vitro Cell Dev Biol
26
, 291
, 1990
|
Bovine Calf (also lamb) |
Oligodendroglia Neural |
|
(see reference) |
Metabolic Properties of Maintained Oligodendroglia Purified from Brain
,
J Biol Chem
253
, 1592
, 1978
|
Bovine Bovine |
Endothelial |
|
MEM |
Characterization of an In Vitro Blood-Brain Barrier Model System for Studying Drug Transport and Metabolism
,
Pharm Res
3 (2)
, 81
, 1986
|
Bovine Bovine |
Endothelial |
|
MEM |
Application of Cultured Endothelial Cells of the Brain Microvasculature in the Study of the Blood-Brain Barrier
,
J Tiss Cul Meth
14
, 217
, 1992
|
Guinea-Pig Guinea-pig, 200-400 g |
Neurons |
|
PIPES saline |
Isolation of Neurons Suitable for Patch-Clamping from Adult Mammalian Central Nervous Systems
,
J Neurosci Methods
16
, 227
, 1986
|
Human Human |
Amyloid Fibrils |
|
50mM Tris |
Preparation of Amyloid Fibrils Seeded from Brain and Meninges
,
Methods Mol Biol
1345
, 299
, 2016
|
Human Human |
Microvessels |
|
DMEM |
Cutlured Human and Canine Endothelial Cells from Brain Microvessels
,
Brain Res Bull
21
, 785
, 1988
|
Human Human, fetal |
Astrocytes |
|
EBSS |
Isolation of Human CD49f Astrocytes and iPSC-Based Neurotoxicity Assays.
, STAR Protoc
Vol. 1
, 100172
, 2020
|
Human Human, 15-54 years |
Microvessels |
|
Serum-free modified Lewis medium |
Isolation and Culture of Cells Derived From Human Cerebral Microvessels
,
Cell Tissue Res
249
, 657
, 1987
|
Human Human |
Neuronal |
|
Tris-HCl, 50 mM, CaCl2, 2 mM | |
Human Human |
Microglia |
|
DMEM/F12 | |
Human Human |
Tumor |
|
see reference |
Efficacy of the HSP90 Inhibitor 17-AAG in Human Glioma Cell Lines and Tumorigenic Glioma Stem Cells.
, Neuro Oncol
Vol. 11
, 109
, 2009
|
Human Human, fetal and mature |
Astrocytes and neurons |
|
RPMI | |
Human Human |
Microglia |
|
Hibernate A | |
Human Human, 25-81 yr |
Brain tumor |
|
HBSS | |
Human |
Brain tumor |
|
DMEM/F12 | |
Human Human |
Microglia |
|
DMEM | |
Human Human, adult |
Neuronal |
|
Neurobasal | |
Insect Drosophila |
Neurons |
|
Saline |
Whole Cell Recordings from Brain of Adult Drosophila.
,
J Vis Exp
6
, 248
, 2007
|
Insect Drosophila |
Neurons |
|
DMEM | |
Insect Drosophilia |
Kenyon |
|
Recording saline |
Cholinergic Synaptic Transmission in Adult Drosophila Kenyon Cells in Situ.
,
J Neurosci
26
, 265-72
, 2006
|
Insect Drosophilia |
Neurons |
|
Modified Dissecting Saline | |
Monkey Monkey, rhesus |
Brain cells |
|
HBSS | |
Mouse Mouse |
Granule cell precursors, pre-neoplastic and tumor cells |
|
Neurobasal/B27 | |
Mouse Mouse |
Neurons |
|
Artificial CSF | |
Mouse Mouse, 6-12 week |
Vascular smooth muscle cells |
|
PBS | |
Mouse Mouse, SWR or CF1, 1-3 months |
Papillae, taste receptor |
|
Carbonate-Phosphate buffer (see reference) |
A Method for Isolating and Patch-Clamping Single Mammalian Taste Receptor Cells
,
Brain Res
503
, 326
, 1989
|
Mouse Mouse, 1-2 day |
Oligodendrocytes, dorsal root ganglia |
|
DMEM | |
Mouse Mouse, 0-30 day |
Neural |
|
BSS |
Cultured Cell Systems and Methods for Neurobiology
,
Vol. 32
, 765
, 1974
|
Mouse Mouse, 4-6 day |
Neurons |
|
HBSS | |
Mouse Mouse |
Astrocytes |
|
DMEM |
Interaction Between Astrocytes and Adult Subventricular Zone Precursors Stimulates Neurogenesis
,
Proc Natl Acad Sci U S A
96
, 7526
, 1999
|
Mouse Mouse, embryonic and postnatal |
Cortical neurons |
|
Neurobasal |
Preparation of Dissociated Mouse Cortical Neuron Cultures.
,
J Vis Exp
10
, 562
, 2007
|
Mouse Mouse, adult |
Interneurons | EBSS |
Protocol for Isolating Young Adult Parvalbumin Interneurons from the Mouse Brain for Extraction of High-Quality RNA.
, STAR Protoc
Vol. 2
, 100714
, 2021
|
|
Mouse Mouse, 3 day |
Microglia |
|
Eagle's MEM |
Involvement of cathepsin E in exogenous antigen processing in primary cultured murine microglia
,
J Biol Chem
277
, 4816
, 2002
|
Mouse Mouse, neonatal |
Neurons |
|
Neurobasal |
Culture of Postnatal Mesencephalic Dopamine Neurons on an Astrocyte Monolayer
, Current Protocols in Neuroscience
Vol. 44
, 3.21.1
, 2008
|
Mouse Mouse, SD, 8-12 week |
Microglia |
|
RPMI-1640 |
IGF-I and microglia/macrophage proliferation in the ischemic mouse brain
,
Glia
39
, 85
, 2002
|
Mouse Mouse, adult |
Neurons |
|
DMEM/F12 | |
Mouse Mouse |
Cerebellar granule cell precursors |
|
EBSS |
A role for p27/Kip1 in the control of cerebellar granule cell precursor proliferation
,
J Neurosci
20
, 5756
, 2000
|
Mouse Mouse |
Brain-sequestered leukocytes |
|
RPMI | |
Mouse Mouse |
Neurosperes |
|
DMEM/F12 | |
Mouse Mouse |
Cerebral pericytes |
|
DMEM |
Pharmacological Inhibition of Mitochondrial Carbonic Anhydrases Protects Mouse Cerebral Pericytes from High Glucose-Induced Oxidative Stress and Apoptosis.
,
J Pharmacol Exp Ther
344
, 637-45
, 2013
|
Mouse Mouse, newborn |
Hippocampal cells |
|
DMKM | |
Mouse Mouse, P13-15 |
Glial |
|
EBSS | |
Mouse Mouse, 2-5 day |
Postnal substantia nigra |
|
see reference |
Method for culturing postnatal substantia nigra as an in vitro model of experimental Parkinson's disease
,
Brain Res Brain Res Protoc
9
, 105-11
, 2002
|
Mouse Mouse |
Chloroid plexus |
|
PBS | |
Mouse Mouse |
Cortical neurons |
|
Neurobasal | |
Mouse Mouse, fetal and mature |
Astrocytes and neurons |
|
RPMI | |
Mouse Mouse, 1 day |
Neurons and glia |
|
Neurobasal |
Proteasomal-Dependent Aggregate Reversal and Absence of Cell Death in a Conditional Mouse Model of Huntington's Disease.
,
J Neurosci
21
, 8772
, 2001
|
Mouse Mouse, 3 week |
Neural |
|
PBS | |
Mouse Mouse, 1 day |
Neural progenitor |
|
see reference | |
Mouse Mouse, P0-P15 |
Astrocytes |
|
DPBS |
Purification of Rat and Mouse Astrocytes by Immunopanning.
,
Cold Spring Harb. Protoc.
2013
, 421-32
, 2013
|
Mouse Mouse, embryonic |
Cortical progenitors |
|
Serum free medium | |
Mouse Mouse, adult |
Astrocytes, microglia, endothelia, oligodendrocytes |
|
HBSS | |
Mouse Mouse, postnatal |
Astrocytes |
|
HBSS |
Differentiation of Neural Stem Cells Into Oligodendrocytes: Involvement of the Polycomb Group Protein Ezh2.
,
Stem Cells
26
, 2875
, 2008
|
Mouse Adult and neonatal |
Microglia, astrocytes, lymphocytes |
|
EBSS | |
Ovine Lamb (also calf) |
Oligodendroglia Neural |
|
(see reference) |
Metabolic Properties of Maintained Oligodendroglia Purified from Brain
,
J Biol Chem
253
, 1592
, 1978
|
Ovine Ovine |
Neurons |
|
DMEM |
Aromatase Immunoreactivity in Fetal Ovine Neuronal Cell Cultures Exposed to Oxidative Injury.
,
Eur J Histochem
53
, e28
, 2009
|
Ovine Ovine |
Neurons |
|
Hibernate | |
Porcine Mini pigs, Yucatan (Susscrofa Yucatan), 4-6 months |
Microvascular |
|
HBSS |
Morphologic Plasticity and Periodicity : Porcine Cerebral Microvascular Cells in Culture
,
In Vitro Cell Dev Biol
26
, 169
, 1990
|
Rat Rat, Holtzmann, 18 day |
Hippocampal neurons |
|
HBSS |
Rat Hippocampal Neurons in Dispersed Cell Culture
,
Brain Res
126
, 397
, 1977
|
Rat Rat, Wistar, 1-3 day |
Hippocampal neurons |
|
DMEM |
Glucose deprivation activates diversity of potassium channels in cultured rat hippocampal neurons
,
Cell Mol Neurobiol
26
, 307-19
, 2006
|
Rat Rat, fetus, 18 day |
Hippocampi |
|
HBSS |
Synaptic Release of Excitatory Amino Acid Neurotransmitter Mediates Anoxic Neuronal Death
,
J Neurosci
4 (7)
, 1884
, 1984
|
Rat Rat, SD, 5 day |
Hippocampal neurons |
|
HBSS |
Development and Selective Neurodegeneration in Cell Cultures From Different Hippocampal Regions
,
Brain Res
490
, 110
, 1989
|
Rat Rat, SD, 19-20 days pregnant |
Neural |
|
DMEM |
Properties of Neurons from Dissociated Fetal Rat Brain in Serum-Free Culture
,
J Neurosci
3 (12)
, 2448
, 1983
|
Rat Rat, SD, 7 day |
Cerebellar granule neurons |
|
PBS | |
Rat Rat, SD, fetal |
Cerebral cortex Hypothalmus |
|
HEPES |
High Molecular Weight Somatostatin Secretion by Cultured Rat Brain Cell
,
Brain Res
239
, 463
, 1982
|
Rat Rat, fetus |
Cortical |
|
Puck's D1 (see reference) |
A Method for Large-scale Production of Mouse Brain Cortical Cultures
,
Brain Res
255
, 361
, 1982
|
Rat Rat, SD, 19-21 day |
Suprachiasmatic nucleus neurons |
|
MEM |
Mammalian Target of Rapamycin Signaling Modulates Photic Entrainment of the Suprachiasmatic Circadian Clock.
,
J Neurosci
30
, 6302
, 2010
|
Rat Rat, SD, newborn |
Glial |
|
MEM, sterile |
The Olfactory Nerve Contains Two Populations of Glia
,
In Vitro
5
, 269
, 1992
|
Rat Rat, SD, 2 days |
Pineal |
|
DMEM, MEM | |
Rat Rat, neonatal |
Astrocytes |
|
EBSS | |
Rat Rat, fetus, 18-20 day |
Hippocampal neurons |
|
HBSS, CMF | |
Rat Rat, SD, E16 |
Cortical neurons |
|
Neurobasal Medium |
SIB-1757 and SIB-1893: selective, noncompetitive antagonists of metabotropic glutamate receptor type 5
,
J Pharmacol Exp Ther
290
, 170
, 1999
|
Rat Rat, Fisher, 7-21 month |
Hippocampal neurons |
|
Hibernate A |
Human Umbilical Cord Blood Cells Have Trophic Effects on Young and Aging Hippocampal Neurons in Vitro.
,
Aging Dis
1
, 173
, 2010
|
Rat Rat, albino, adult and newborn |
Cerebral cortices |
|
PBS |
Characterization of Ameboid Microglia Isolated From Developing Mammalian Brain
,
J Neurosci
6
, 2163
, 1986
|
Rat Rat, Wistar, newborn |
Germinal matrix |
|
HBSS |
Differentiation of Astrocytes and Oligodendrocytes From Germinal Matrix Cells in Primary Culture
,
J Neurosci
6
, 52
, 1986
|
Rat Rat, Wistar, 14 day |
Visual cortical |
|
EBSS | |
Rat Rat, neonatal |
Dura mater |
|
DMEM | |
Rat Rat, Wistar-Kyoto, male, 100 - 200 g |
Endothelial Cerebral |
|
HBSS |
Primary Culture of Rat Cerebral Microvascular Endothelial Cells
,
Lab Invest
46
, 554
, 1982
|
Rat Rat, Wistar, male |
Cerebral endothelial |
|
MEM |
Heparan sulfate proteoglycans modulate monocyte migration across cerebral endothelium
,
J Neuropathol Exp Neurol
62
, 780
, 2003
|
Rat Rat, SD |
Hippocampal |
|
Neurobasal | |
Rat Rat, 60-72 hours old |
Fibroblasts |
|
HEPES buffered DMEM | |
Rat Rat, wistar, neonatal |
Hippocampal cells |
|
DMEM |
Somatostatin inhibits excitatory transmission at rat hippocampal synapses via presynaptic receptors
,
J Neurosci
17
, 4066
, 1997
|
Rat Rat, SD, 1-2 day |
Oligodendrocyte precursor cells |
|
HBSS | |
Rat Rat, Wistar, adult, 170 g |
Endothelial |
|
BSS |
Isolation And Characterization of Endothelial Cells From Rat And Cow Brain White Matter
,
J Anat
129
, 261
, 1979
|
Rat Rat, wistar, 250-300 g |
Cerebral artery smooth muscle cells |
|
Physiological Salt Solution | |
Rat Rat, P0-P15 |
Astrocytes |
|
DPBS |
Purification of Rat and Mouse Astrocytes by Immunopanning.
,
Cold Spring Harb. Protoc.
2013
, 421-32
, 2013
|
Rat Rat, Wistar, pregnant |
Glial |
|
Eagle's MEM/DMEM | |
Rat Rat, SD, Fisher |
Hippocampal neurons |
|
HibernateA/B27 |
Isolation and culture of adult rat hippocampal neurons of any age
,
J Neurosci Methods
71
, 143
, 1997
|
Rat Rat, SD, E18 |
Neurons |
|
HBSS | |
Rat Rat, SD, adult |
Endothelial |
|
Medium 199 | |
Rat Rat, E18 |
Hippocampal and cortical neurons |
|
Neurobasal media |
Neuritin: a gene induced by neural activity and neurotrophins that promotes neuritogenesis
,
Proc Natl Acad Sci U S A
94
, 2648-53
, 1997
|
Rat Rat, SD, neonatal |
Neurons |
|
Neuro medium | |
Rat Rat, SD, female |
Fetal rat brain |
|
DMEM |
Serum Proteins Enhance Aggregate Formation of Dissociated Fetal Rat Brain Cells in an Aggregating Culture
,
In Vitro Cell Dev Biol
24 (10)
, 1031
, 1988
|
Rat Rat, SD, E18 |
Cortical cells |
|
EBSS |
GABA(B) receptors mediate motility signals for migrating embryonic cortical cells
,
Cereb Cortex
11
, 744-53
, 2001
|
Rat Rat, Wistar, male, 300-500 g |
Microvessels Endothelial |
|
Ringers-HEPES buffer |
Isolation and Characterization of Brain Endothelial Cells: Morphology and Enzyme Activity
,
J Neurochem
35 (2)
, 374
, 1980
|
Rat Rat, E18 |
Hippocampal neurons |
|
Hibernate | |
Rat Rat, SD, one month old |
Capillary endothelium Pericytes |
|
Medium 199 |
Primary Culture of Capillary Endothelium From Rat Brain
,
In Vitro
17 (4)
, 353
, 1981
|
Rat Rat, E19 |
Hippocampal neuron |
|
MEM | |
Rat Rat, newborn |
Astrocytes |
|
DMEM, HBSS | |
Rat Rat, neonatal |
Hippocampal neurons |
|
EBSS | |
Shellfish Helisoma trivolvis |
Neurons Buccal ganglia |
|
Saline, Sterile |
Formation of Electrical Synapses Between Isolated, Cultured Helisoma Neurons Requires Mutual Neurite Elongation
,
J Neurosci
5
, 3145
, 1985
|