Epithelial Tissue Tables

Species Cells Enzyme(s) Medium Reference
Bovine
Bovine, fetal
Epithelial
Tracheal
Dissociation medium, CMF
Schumann, B.L. , Cody, T.E. , Miller, M.L. and Leikauf, G.D.
Canine
Dog
Tracheal
  • Pronase : 0.1%
DMEM
Virmani, A. , Naziruddin, B. , Desai, V. , Lowry, J. , Graves, D. and Sachdev, G.
Chicken
Chick, 5 day old
Intestinal mesenchymal and epithelial DMEM
Simon-Assmann, P. and Kedinger, M
Embryonic Gut-Dissagregated Culture , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12A:3.1 , 1995
Fish
Shark (Squalus acanthias)
Rectal gland Ringer's solution
Fish
Shark (Squalus acanthias)
Rectal gland Ringer's solution
Karnaky, Jr. , Valentich, J.D. , Currie, M.G. , Oehlenschlager, W.F. and Kennedy, M.P.
Fish
Winter flounder, 200-500 g
(Pseudopleuronectes americanus)
Renal tubule CMF solution
Dickman, K. and Renfro, J.
Frog
Frog, Xenopus laevis, adult, female
Colonic epithelial Kreb's
Heinke, B. and Clauss, W.
Guinea-Pig
Guinea-pig, Hartley albino,
500-600 g
Endometrial HBSS
Chaminadas, G. , Alkhalaf, M. , Remy-Martin, J.P. , Propper, A.Y. and Adessi, G.L.
Guinea-Pig
Guinea-pig, Hartley, female, 200 g
Epithelial DMEM
Hamster
Hamster, Syrian golden (strain CR:RGH)
Tracheal Ham's F-12
Hamster
Hamster (strain 1516 EHS and Lakeview), 8-12 weeks
Tracheal Medium 199
Lee, T. , Wu, R. , Brody, A. , Barrett, J. and Nettesheim, P.
Hamster
Hamster, Syrian gold, male, 100-120 g
Tracheal
  • Pronase : 0.1%
MEM with Hepes, CMF
Niles,R. , Kim, K.C. , Hyman, B. , Christensen, T. , Wasano, K. and Brody, J.
Hamster
Hamster, Syrian gold, male, 6 weeks- 4 months
Tracheal PBS with EDTA
Human
Human
Epithelial DMEM
Sabatini, L. , Allen-Hoffmann, B, Warner, T. and Azen, E.
Human
Human
Epithelial MEM, PBS
Robinson, C. and Wu, R.
Human
Human
Gastric F-12 medium
Sarosiek, J, Marshall, B, Peura, D, Guerrant, L, McCallum, R. and Little, C
The Isolation and Maintenance of Human Gastric Epithelial Cells in Primary Culture , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12B:10.1 , 1995
Human
Human
Human tracheal epithelium
  • Protease Type XIV : 0.04%
DMEM/F12
Human
Human
Corneal limbal epithelial sheet see reference
Espana EM, Romano AC, Kawakita T, Di Pascuale M, Smiddy R. and Tseng SC
Human
Human
Nasal polyp epithelial see reference
Halbert CL, Alexander IE, Wolgamot GM, Miller AD.
Human
Human
Colonic epithelial RPMI 1640
Human
Human
Intestinal epithelial HBSS
Fahlgren, A. , Hammarstrom, S. , Danielsson, A. and Hammarstrom, M.
Human
Human
Epithelial DMEM/Ham's F-12
Human
Human
Gastric epithelial cells L-15
Smoot, D. , Sewchand, J. , Young, K. , Desbrodes, B. , Allen, C. and Naab, T.
Human
Human
Epithelial
  • Pronase : 0.1%
PBS
Gruenert, D.C. , Basbaum, C.B. and Widdicombe, J.H.
Human
Human
Oral mucosal epithelial DMEM/F12
Hsueh, Y. , Huang, S. , Lai, J. , Ma, S. , Chen, H. , Wu, S. , Wang, T. , Sun, C. , Ma, K. , Chen, J. , Lai, C. and Ma, D.
Human
Human, women, 27-49 years
Epithelial
Ovary
HBSS, CMF
Auersperg, N. , Siemens, C.H. and Myrdal, S.E.
Human
Human
Epithelial PBS
Gottipamula, S. , Saraswat, S. and Sridhar, K.
Human
Human
Epithelial HEPES with 5.9mM Glucose,5mM DTT
Widdicombe, J.H. , Coleman, D.L. , Finkbeiner, W.E. and Tuet, I.K.
Human
Human
Gastrointestinal epithelial DMEM/F12
VanDussen, K. , Marinshaw, J. , Shaikh, N. , Miyoshi, H. , Moon, C. , Tarr, P. , Ciorba, M. and Stappenbeck, T.
Human
Human
Epithelial
Sweat gland
(see reference)
Human
Human, infant and neonate
Epithelial
Prostate
HBSS
Lechner, J. , Babcock, M. , Marnell, M. , Narayan, K. and Kaighn, M.
Normal Human Prostate Epithelial Cell Cultures ,
Methods Cell Biol
21
, 195 , 1980
Human
Human
Epithelial
  • Protease Type XIV : 0.1%
Eagle's MEM
Yankaskas, J. , Cotton, C. , Knowles, M. , Gatzy, J. and Boucher, R.
Human
Human
(also bovine)
Endometrial epithelial DMEM/Ham's F-12
Munson, L. , Chandler, S. and Schlafer, D.
Mouse
Mouse, (BALB/cfC3H or BALB/c) 8-12 day mid-pregnant
Epithelial
Submandibular gland
HBSS
Yang, J. , Flynn, D. , Larson, L. and Hamamoto, S.
Mouse
Mouse, female
Epithelial HBSS with 0.2% EDTA, CMF
Riser, M. , Huff, B. and Medina, D.
Mouse
Mouse, mature, female, 6-8-wk-old
Uterine HBSS
Ghosh, D. , Danielson, K. , Alston, J. and Heyner, S.
Mouse
Mouse, female, 18 - 20 days
(also 20 - 22 days)
Uterine HBSS
Mouse
Mouse, fetal 12-13 day
Intestinal mesenchymal and epithelial DMEM
Simon-Assmann, P. and Kedinger, M
Embryonic Gut-Dissagregated Culture , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12A:3.1 , 1995
Mouse
Mouse
Submandibular salivary DMEM
Durban, E.
Submandibular Salivary Epithelial Cells , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12B:2.1 , 1995
Mouse
Mouse, 6-8 week
Lamina propria mononuclear cells RPMI
Wirtz S. , Becker C. , Blumberg R. , Galle P. and Neurath M.
Mouse
Mouse, female
Salivary gland epithelial DMEM/F12
Ishimaru N, Saegusa K, Yanagi K, Haneji N, Saito I, Hayashi Y.
Mouse
Mouse, 11 week
Epithelial DMEM/F12
Mueller, S. , Clark, J. , Myers, P. and Korach, K.
Mouse
Mouse, male, 8-16 week
Renal tubular epithelial HBSS
Mouse
Mouse, adult
Cilary epithelial EBSS
Gualdoni, S. , Baron, M. , Lakowski, J. , Decembrini, S. , Pearson, R. , Ali, R. and Sowden, J.
Mouse
Mouse
Tracheal epithelial See reference
Pardo-Saganta, A. , Tata, P. , Law, B. , Saez, B. , Chow, R. , Prabhu, M. , Gridley, T. and Rajagopal, J.
Mouse
Mouse
Epithelial DMEM
Mathew, R. , Degenhard,t K. , Haramaty, L. , Karp, C. and White, E.
Mouse
Mouse, (BALB/c), male, 3-5 months
Epithelial
Submandibular salivary gland
DMEM with 15 mM HEPES
Mouse
Mouse
Alveolar epithelial DMEM
Mouse
Mouse
Esophageal PBS, CMF
Mouse
Mouse, 8-12 week
Nasal epithelium EBSS
Brann, D. , Tsukahara, T. , Weinreb, C. , Lipovsek, M. , Van den Berge, K. , Gong, B. , Chance, R. , Macaulay, I. , Chou, H. , Fletcher, R. , Das, D. , Street, K. , de Bezieux, H. , Choi, Y. , Risso, D. and Dudoit, S.
Mouse
Mouse, C3H, 6-8 weeks
Epithelial DMEM
Lillehaug, J. , Mondal, S. and Heidelberger, C.
Mouse
Mouse (BALB/cfC3H)
Mammary tumors
Epithelial
HBSS
Yang, J. , Guzman, R. , Richards, J. and Nandi, S.
Porcine
Porcine, 5-60 kg
Retinal pigment epithelial cells DMEM
Wiencke, A. , Kiilgaard, J. , Nicolini, J. , Bundgaard, M. , Ropke, C. and La Cour, M.
Growth of Cultured Porcine Retinal Pigment Epithelial Cells ,
Acta Opthalmol Scand
81(2)
, 170 , 2003
Porcine
Porcine
Trachea HBSS
De Buysscher, E. , Kennedy, J. and Mendicino, J.
Rabbit
Rabbit, New Zealand white, male, 4-5lb.
Gastric
Parietal and chief
Sodium phosphate buffer
Rabbit
Rabbit, fetal
Epithelial
Gastric
HBSS
Logsdon, C.D. , Bisbee, C.A. , Rutten, M.J. and Machen, T.E.
Rabbit
Rabbit, New Zealand white estrous, female, 4-5 months
Mesothelial and surface epithelial
Ovaries
Medium 199
Nicosia, S. , Johnson, J. and Streibel, E.
Rabbit
Rabbit, New Zealand white, adult
Colon PBS
Vidrich, A. , Racindranath, R. , Farsi, K. and Targan, S.
Rat
Rat (ACI/NMs X BUF/Mna) F1, male, 28 months
Rat (ACI/MNs) male, 8 weeks
Epthelial Eagle's MEM Serum-free
Masuda, A. , Ohtsuka, K. and Matsuyama, M.
Rat
Rat, Fischer, adult, 200-250 g
Epithelial KCl-NaCl HEPES Buffer
Rat
Rat, Fischer, 10 day
Epithelial-like PBS
Williams, G. , Weisburger, E. and Weisburger, J.
Rat
Rodent, various (see reference)
Epithelial MEM, PBS
Robinson, C. and Wu, R.
Rat
Rat, SD, female
Epithelial, cancer and tumor Eagles's MEM
Rat
Rat, Wistar, 12 day
Epithelial HBSS, CMF
Rat
Rat, 4-14 week
Retinal pigment epithelial cells CF Hanks with EDTA
Wang N, Koutz CA. and Anderson RE.
Rat
Rat, SD, male, 150 - 250 g
Epithelial
Stomach
  • Pronase : 0.15%
Medium 199
Rat
Rat, male
Alveolar epithelial DMEM
Planus, E. , Galiacy, S. , Matthay, M. , Laurent, V. , Gavrilovic, J. , Murphy, G. , Clerici, C. , Isabey, D. , Lafuma, C. and d'Ortho, M.
Rat
Rat, Fischer, male, 4-6 weeks
Epithelial
Esophagus
HEPES BSS
Babcock, M. , Marino, M. , Gunning, W. and Stoner, G.
Rat
Rat, 6 day old
Rat intestinal epithelial DMEM
Rat
Rat, Fischer, Lewis and SD, male, 10-18 months
Epithelial HBSS CMF
Herring, A. , Raychaudhuri, R. , Kelley, S. and Iybe, P.
Rat
Rat, 6-8 day
Retinal pigment epithelial DMEM
Chang CW, Roque RS, Defoe DM. and Caldwell RB.
Rat
Rat, adult
(also hamster)
Interlobular duct fragments DMEM/Ham's F-12
Rat
Rat, embryonic
Tracheal epithelial DMEM/F12
Rat
Rat, SD, adult, 90-120 day old, 350-450 g
Epididymal epithelial HBSS
Rat
Rat
Mammary epithelial HBSS
Mei, N. , McDaniel, L. , Dobrovolsky, V. , Guo, X. , Shaddock, J. , Mittelstaedt, R. , Azuma, M. , Shelton, S. , McGarrity, L. , Doerge, D. and Heflich, R.
Rat
Rat, Fisher 344, male, 8 wks old,
250-300 g
Tracheal epithelial
  • Pronase : 0.5%
DMEM
Rat
Rat, SD, 200 g
Colon (see reference)
Yassin, R. , Clearfield, H. , Katz, S. and Murthy, S.
Rat
Rat, SD, adult, male, 8-10 weeks old
Seminiferous tubules Krebs-Ringer bicarbonate buffer
(see reference)
Rat
Rat, fetal, 14-15 day
Intestinal mesenchymal and epithelial DMEM
Simon-Assmann, P. and Kedinger, M
Embryonic Gut-Dissagregated Culture , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12A:3.1 , 1995
Rat
Rat, Wistar, neonatal
Epithelial HBSS
Jassal, D. , Han, R. , Caniggia, I. , Post, M. and Tanswell, A.

Tissue Tables (references, grouped by tissue type and species)