Muscle Tissue Tables

Species Cells Enzyme(s) Medium Reference
Bovine
Bovine
Smooth muscle DMEM
Absher, M. , Woodcock-Mitchell, J. , Mitchell, J. , Baldor, L. , Low, R. and Warshaw, D.
Bovine
Bovine
Vascular smooth muscle PSS
Warshaw, D. , Szarek, J. , Hubbard, M. and Evans, J.
Bovine
Bovine
Smooth muscle, fibroblasts DMEM
Bovine
Bovine
Pulmonary artery endothelial and smooth muscle cells RPMI-1640
Yu, M. , McAndrew, R. , Al-Saghir, R. , Maier, K. , Medhora, M. , Roman, R. and Jacobs, E.
Bovine
Bovine
Muscle satellite cells Krebs-Ringer Bicarbonate
Lee, E. , Choi, J. , Hyun, J. , Cho, K. , Hwang, I. , Lee, H. , Chang, J. and Choi, I.
Canine
Dog, beagle, adult
Smooth muscle
Vascular
Tyrode's solution w/ calcium
Canine
Dog
Artery
Carotid
PSS
Canine
Dog
Smooth muscle PSS
Subramanian, M. , Madden, J. and Harder, D.
Canine
Canine
Skeletal muscle DMEM
Parker, M. , Loretz, C. , Tyler, A. , Snider, L. , Storb. R. and Tapscott, S.
Chicken
Chick embryonic
Various tissues (heart, liver, skeletal, cardiac) CMF Tyrode's solution
Chicken
Chick embryo
Thyroid
Muscle
Heart
Tyrode's saline, potassium free
Chicken
Chick, white leghorn, 12 day
Muscle Puck's saline A
Chicken
Chick, white leghorn, embryos, 11 day
Muscle Saline G
Tepperman, K. , Morris, G. , Essien, F. and Heywood, S.M.
Chicken
Chick
Smooth muscle HBSS
Chamley-Campbell, J. , Campbell, G. and Ross, R.
The Smooth Muscle in Cell Culture ,
Physiol Res
59
, 1 , 1979
Chicken
Chick, embryonic
Muscle CMF HBSS
Hilfer, S.
Collagenase Treatment of Chick Heart and Thyroid , Tissue Cult Methods & Applications , Kruse, P., and Patterson, M. , 246 , 1971
Chicken
Chicken, 1-2 day
Gizzard and aorta smooth muscle HBSS
Dirksen W. , Vladic F. and Fisher S.
Feline
Cat, mongrel, adult, 2-4 kg
Myocytes Kreb's Henseleit, CF
Follmer, C.H. , Ten Eick, R.E. and Yeh, J.Z.
Feline
Cat, adult mongrel, either sex, 2.5-4.0 kg
Cerebral arteries Puck's solution
Madden, J. , Vadula, M. and Kurup, V.
Fish
Dogfish, Squalus acanthias , 2-6 Kg
(also guinea-pig, rabbit, frog)
Myocytes, heart and stomach
  • Protease XIV : 0.028%
Solution C
(see reference)
Fish
Zebrafish
Myogenic progenitor PBS
Alexander, M. , Kawahara, G. , Kho, A. , Howell, M. , Pusack, T. , Myers, J. , Montanaro, F. , Zon, L. , Guyon, J. and Kunkel, L.
Frog
Frog
Myocytes CF Ringer
Shepherd, N. and Kavaler, F.
Frog
Frog, Xenopus laevis, embryos stage 17 & 19
Muscle Steinberg's solution
Frog
Frog, Xenopus laevis
Muscle L15 medium
(see reference)
Frog
Frog, Rana pipiens (50-100 g)
(also guinea-pig, rabbit, dogfish, Squalus acanthias )
Myocytes, heart and stomach
  • Protease XIV : 0.028%
Solution C
(see reference)
Guinea-Pig
Guinea-pig, 200-400 g
(also rabbit, frog, dogfish)
Myocytes, heart and stomach
  • Protease XIV : 0.028%
Solution C
(see reference)
Guinea-Pig
Guinea-pig, 200-300 g
Smooth muscle
Mesenteric artery
CF solution
Ohya, Y. and Sperelakis, N.
Guinea-Pig
Guinea-pig
(also rat, rabbit)
Smooth muscle Potassium buffer solution
Guinea-Pig
Guinea-pig, prepubertal
Smooth muscle
Aortic
Dulbecco-Vogt modification of Eagle's
Guinea-Pig
Guinea-pig, 200-380 g
Capillaries
Myocytes
CF solution
Schnitzler, M. , Derst, C. , Daut, J. and Preisig-Muller, R.
Guinea-Pig
Guinea-pig, Dunkin-Hartley, female
Myocytes
  • Protease
DMEM
Guinea-Pig
Guinea-pig, adult, 250-350 g
Smooth muscle
Gallbladder
NaCl, sodium glutamate, MgCl, KCl, glucose, Kreb's, and HEPES
Jennings, L. , Xu, Q. , Firth, T. , Nelson, M. and Mawe, G.
Guinea-Pig
Guinea pig, 2-4 wk old, male, female
Smooth muscle
Gallbladder
Krebs solution
Guinea-Pig
Guinea-pig
Bladder smooth muscle Krebs-Ringer bicarbonate
Shieh CC, Feng J, Buckner SA, Brioni JD, Coghlan MJ, Sullivan JP, Gopalakrishnan M.
Hamster
Hamster, male, 60-70 day
Satellite DMEM
Nakamura, T. , Iwata, Y. , Sampaolesi, M. , Hanada, H. , Saito, N. , Artman, M. , Coetzee, W. and Shigekawa, M.
Human
Human
(also rat, guinea-pig, chick, monkey)
Smooth muscle HBSS
Chamley-Campbell, J. , Campbell, G. and Ross, R.
The Smooth Muscle in Cell Culture ,
Physiol Res
59
, 1 , 1979
Human
Human
Smooth muscle DMEM
Eskin, S. , Sybers, H. , Lester, J. , Navarro, L. , Gotto, A. and DeBakey, M.
Human
Human
Muscle derived multiprogenitor cells DMEM
Nesti, L. , Jackson, W. , Shanti, R. , Koehler, S. , Aragon, A. , Bailey, J. , Sracic, M. , Freedman, B. , Giuliani, J. and Tuan, R.
Human
Human
Urinary tract smooth muscle DMEM
Kimuli, M. , Eardley, I. and Southgate, J.
Human
Human
Endothelial and vascular smooth muscle HBSS
Human
Human, male
Myogenic HBSS
Stadler, G. , Chen, J. , Wagner, K. , Robin, J. , Shay, J. , Emerson, C. and Wright, W.
Human
Human
Smooth muscle DMEM
Lu, S. , Sun, X. , Hong, T. , Song, K. , Yang, S. and Wang, C.
Human
Human
Skeletal muscle stem Ham's F10
Charville, G. , Cheung, T. , Yoo, B. , Santos, P. , Lee, G. , Shrager, J. and Rando, T.
Human
Human
Muscle satellite DMEM
Garcia, S. , Tamaki, S. , Lee, S. , Wong, A. , Jose, A. , Dreux, J. , Kouklis, G. , Sbitany, H. , Seth, R. , Knott, P. , Heaton, C. , Ryan, W. , Kim, E. , Hansen, S. , Hoffman, W. and Pomerantz, J.
Human
Human
Bladder smooth muscle Digestion solution (see reference)
Human
Human, fetal
(also bovine)
Smooth muscle, fibroblasts DMEM
Human
Human, female
Smooth muscle
Myometrial
HBSS
Richardson, M. , Taylor, D. , Casey, M. , MacDonald, P. and Stull, J.
Human
Human, female
Smooth muscle
Myometrial
HBSS
Casey, M. , MacDonald, P. , Mitchell, M. and Snyder, J.
Lizard
Lizard (Anolis carolinensis)
Myoblasts, tail GM III
(see reference)
Cox, P. and Simpson, Jr., S.
Monkey
Rhesus monkey, 1 year
(also human, rabbits)
Smooth muscle, saphenous vein BSS
Chamley, J. , Campbell, G. , McConnell, J. and Groschel-Stewart, U.
Monkey
Monkey (Macaca nemestrina)
Smooth muscle Dulbecco-Vogt
Chait, A. , Ross, R. , Albers, J. and Bierman, E.
Mouse
Mouse, 6-8 week
Myocytes HBSS
McKinney-Freeman SL, Jackson KA, Camargo FD, Ferrari G, Mavilio F, Goodell MA.
Mouse
Mouse, 1-25 month
Myocytes, endothelial PBS
Mouse
Mouse, neonatal
Skeletal muscle myotubes L-15
Johnson, B. , Scheuer, T. and Catterall, W.
Mouse
Mouse, male, 6-14 week
Precursor cells DMEM/F12
Winitsky, S. , Gopal, T. , Hassanzadeh, S. , Takahashi, H. , Gryder, D. , Rogawski, M. , Takeda, K. , Yu, Z. , Xu, Y. and Epstein, N.
Mouse
Mouse
Myoblast DMEM
Shi, H. , Boadu, E. , Mercan, F. , Le, A. , Roth Flach, R. , Zhang, L. , Tyner, K. , Olwin, B. and Bennett, A.
Mouse
Mouse
Vascular smooth muscle Basal Culture
Metz, R. , Patterson, J. and Wilson, E.
Mouse
Mouse, 6-8 week
Myofibers DMEM
Mouse
Myofiber DMEM
Huttner,S. , Ahrens, H. , Schmidt, M. , Henze, H. , Jung, M. , Schuler. S. and von Maltzahn, J.
Mouse
Mouse, 10 week
Diaphragm cells PBS
Rezk, B. , Yoshida, T. , Semprun-Prieto, L. , Higashi, Y. , Sukhanov, S. and Delafontaine, P.
Mouse
Mouse
Skeletal muscle fiber Tyrode
Weisleder, N. , Zhou, J. and Ma, J.
Mouse
Mouse, GFP transgenic
Muscle derived NLSC DMEM
Birbrair, A, Sattiraju, A, Zhu, D, Zulato, G, Batista, I, Nguyen, V, Messi M, Solingapuram Sai, K, Marini, F, Delbono, O. and Mintz, A.
Mouse
Mouse, 2-4 month
Pharyngeal myofiber DMEM
Randolph, M. , Phillips, B. , Choo, H. , Vest, K. , Vera, Y. and Pavlath, G.
Mouse
Mouse, 2 month
Myoblasts Hams F-10
Abou-Khalil, R. , Yang, F. , Lieu, S. , Julien, A. , Perry, J. , Pereira, C. , Relaix, F. , Miclau, T. , Marcucio, R. and Colnot, C.
Mouse
Mouse, 6-8 week
Skeletal muscle progenitor DMEM
Majka S. , Jackson K. , Kienstra K. , Majesky M. , Goodell M. and Hirschi K.
Mouse
Mouse, 6-8 week
vascular smooth muscle DMEM/F12
Sachdeva, J. , Mahajan, A. , Cheng, J. , Baeten, J. , Lilly, B. , Kuivaniemi, H. and Hans, C.
Mouse
Mouse, neonatal
Myocytes DMEM
Fukada S. , Miyagoe-Suzuki Y. , Tsukihara H. , Yuasa K. , Higuchi S. , Ono S. , Tsujikawa K. , Takeda S. and Yamamoto H.
Mouse
Mouse
Extraocular muscles Ringer
Eckhardt, J. , Bachmann, C. , Sekulic-Jablanovic, M. , Enzmann, V. , Park, K. , Ma, J. , Takeshima, H. , Zorzato, F. and Treves, S.
Mouse
Mouse, 15-20 day
Intersitial cells of Cajal M199
Ovine
Sheep, adult and neonatal
Tracheal smooth muscle cells MOPS-PSS
Driska S. , Laudadio R. , Wolfson M. and Shaffer T.
Porcine
Porcine
Smooth muscle, aorta DMEM
Xiong, Yimin, Xu, Shangzhe. and Slakey, Linda L
Porcine
Porcine
Smooth muscle
Aortic medial tissue
DMEM
Fehr, T. , Dickinson, E. , Goldman, S. and Slakey, L.
Porcine
Porcine, adult, 35-45 kg
Arterial smooth muscle MEM
Porcine
Porcine, Yorkshire, 30kg
Coronary smooth muscle HBSS
Porcine
Porcine, male, 25-40 kg
Coronary myocytes low calcium physiological saline
Porcine
Porcine, juvenile, 30kg
Coronary smooth muscle cells HBSS
Huckle WR, Drag MD, Acker WR, Powers M, McFall RC, Holder DJ, Fujita T, Stabilito II, Kim D, Ondeyka DL, Mantlo NB, Chang RS, Reilly CF, Schwartz RS, Greenlee WJ, Johnson RG.
Porcine
Porcine
Bladder smooth muscle Krebs-Ringer bicarbonate
Shieh CC, Feng J, Buckner SA, Brioni JD, Coghlan MJ, Sullivan JP, Gopalakrishnan M.
Porcine
Porcine, 2 month
Skeletal muscle HBSS
Lewis, F. , Henning, B. , Marazzi, G. , Sassoon, D. , Ellison, G. and Nadal-Ginard, B.
Porcine
Porcine, postnatal
Muscle satellite Eagle MEM
Porcine
Porcine
Smooth muscle
Aorta
EDTA 0.02%
Breton, M. , Berrou, E. , Deudon, E. and Picard, J.
Quail
Quail, embryo, 10 day
Myoblasts Puck's solution
Konigsberg, I.
Rabbit
Rabbit, white New Zealand, adult, male, 2 Kg
Smooth muscle, aorta Krebs Ringer HEPES solution
Ives, H. , Schultz, G. , Galardy, R. and Jamieson, J.
Rabbit
Rabbit, adult, 1-2 Kg
Smooth muscle, ear artery CF solution
(see reference)
Rabbit
Rabbit, New England, albino
Thoracic aorta Kreb's Ringer
Day, A. , Phil, D. and Newman, H.
Rabbit
Rabbit, adult, 1-2 kg
Smooth muscle Saline
Rabbit
Rabbit, New Zealand White
Aortic smooth muscle F10 Ham's
Croons Valerie, Martinet Wim, Herman Arnold G, Timmermans Jean-Pierre, De Meyer Guido R Y.
Rabbit
Rabbit, New Zealand
Gastric smooth muscle DMEM
Rabbit
Rabbit, 0.5-1 Kg
(also guinea-pig, frog, dogfish)
Myocytes, heart and stomach
  • Protease XIV : 0.028%
Solution C
(see reference)
Rabbit
Rabbits, New Zealand white albino, 5-6 months
(also human, Rhesus monkey, rabbit)
Smooth muscle, saphenous vein BSS
Chamley, J. , Campbell, G. , McConnell, J. and Groschel-Stewart, U.
Rabbit
Rabbit, New Zealand, male, 2.5-3.5 Kg
Enterocytes RPMI 1640 w/ 1% fetal bovine serum
PBS
Santos, M. , Nguyen, B.. and Thompson, J.
Rabbit
Rabbit
(also rat, guinea-pig)
Smooth muscle Potassium buffer solution
Rabbit
Rabbit, New Zealand white, 1500 g
Smooth muscle, aortic MEM
Knodle, S. , Anderson, S. and Papaioannou, S.
Rabbit
Rabbit, chinchilla, 5-8 month, virgin, female, 2-3 kg
Smooth muscle, aorta HBSS
Rat
Rat, male, 150-250 g
Endothelial, aortic RPMI 1640
Cole, O. , Fan, T. and Lewis, G.
Rat
Rat, day 7
Skeletal muscle DMEM
Vilmont, V. , Cadot, B. , Ouanoumou, G. and Gomes, E.
Rat
Rat, Wistar, adult, male
Smooth & skeletal muscle
Cardiac myocytes
  • Protease : 0.01%
PSS
Wellman, G. , Barrett-Jolley, R. , Koppel, H. Everitt, D. and Quayle, J.
Rat
Rat
Vascular smooth muscle DMEM
Weber, S. , Gratopp, A. , Akanbi, S. , Rheinlaender, C. , Sallmon, H. , Barikbin, P. and Koehne, P.
Rat
Rat, SD, adult and neonatal w/in 1 day of birth
Myooids Ham's F-12
Rat
Rat, SD, male, 150-175 g
Smooth muscle, endothelial MEM
Redmond, E. , Cahill, P. and Sitzmann, J.
Rat
Rat, 3-4 day
Myocardial HBSS CMF
Rat
Rat, Wistar, 3-10 day
Heart Saline A
(See reference)
Rat
Rat, 1-3 day
Smooth muscle, aortic DMEM
Barone, L. , Wolfe, L. , Faris, B. and Franzblau, C.
Rat
Rat, SD, 3 month
Smooth muscle cells M-199
Stevenson S. , Rollence M. , Marshall-Neff J. and Liau G.
Rat
Rat, 200 g
Muscle Kreb's Henseleit bicarbonate buffer
Rat
Rat, SD, male, 250-350 g
Vascular smooth muscle DMEM
Hrometz, S. , Edelmann, S. , McCune, D. , Olges, J. , Hadley, R. , Perez ,D. and Piascik, M.
Rat
Rat
Muscle, mesenteric arteries HEPES KG solution
(see reference)
Bean, B. , Sturek, M. , Puga, A. and Hermsmeyer, K.
Rat
Rat, SD, 225-250g
Mesenteric artery smooth muscle cells HBSS
Gunther S, Alexander RW, Atkinson WJ. and Gimbrone MA Jr.
Rat
Rat
(also rabbit, guinea-pig)
Smooth muscle Potassium buffer solution
Rat
Rat, SD, 250 g
Arterial smooth muscle see reference
Rat
Rat, Wistar, female, 10 weeks
Myocytes HBSS
Boulanger-Saunier, C. , Kattenburg, D. and Stoclet, J.
Rat
Rat, Lewis, neonatal
Myoblasts Ham's F-10
Kim, J. , Hadlock, T. , Cheney, M. , Varvares, M. and Marler, J.
Muscle Tissue Engineering for Partial Glossectomy Defects. ,
Arch Facial Plast Surg
5
, 403 , 2003
Rat
Rat, SD, 19 days
Smooth muscle, myometrial HBSS or PSS, CMF
Loch-Caruso, R. , Pahl, M. and Juberg, D.
Rat
Rat, fetal 17-18 day
Myotubes see reference
Rat
Rat, Wistar Kyoto, 10-15 weeks
Smooth muscle, mesenteric artery MEM
McGuire, P. , Walker-Caprioglio, H. , Little, S. and McGuffee, L.
Rat
Rat
Myogenic Ham's F-10
Rat
Rat, SD, male, 200 - 250 g
Smooth muscle, thoracic aorta Eagle's MEM with calcium
Brock, T. , Alexander, R. , Ekstein, L. , Atkinson, W. and Gimbrone, M.
Rat
Rat
myogenic DMEM
Larkin, L. , Calve, S. , Kostrominova, T. and Arruda, E.
Rat
Rat, SHRs and WKY, male, 10 - 14 weeks
Smooth muscle, tail arteries HEPES buffer
(see reference)
Rat
Rat
Skeletal muscle fiber Tyrode
Weisleder, N. , Zhou, J. and Ma, J.
Rat
Rat, SHR, WKY. either sex, 12-19 day, 3 month, and retired breeders
Endothelial, aortic Waymouth's culture medium
Gordon, D. , Mohai, L. and Schwartz, S.
Rat
Rat, SD
Pulmonary arterial smooth muscle Buffer
Zeng, Y. , Liu, H. , Kang, K. , Wang, Z. , Hui, G. , Zhang, X. , Zhong, J. , Peng, W. , Ramchandran, R. , Raj, J. and Gou, D.

Tissue Tables (references, grouped by tissue type and species)