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Heart Tissue Tables

Species Cells Enzyme(s) Medium Reference
Bovine
Bovine
(also rat) 		Heart
Adrenal chromaffin
Paraneurons 		25mM HEPES buffered Locke's solution, CMF
Trifaro, J. , Tang, R. and Novas, M.
Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons ,
In Vitro Cell Dev Biol
 26 , 335 , 1990
Canine
Canine 		Atrial myocytes Tyrode's solution
Gan, T. , Qiao, W. , Xu, G. , Zhou, X. , Tang, B. , Song, J. , Li, Y. , Zhang, J. , Li, F. , Mao, T. and Jiang, T.
Aging-Associated Changes in L-type Calcium Channels in the Left Atria of Dogs. ,
Exp Ther Med
 6 , 919-924 , 2013
Canine
Canine 		Cardiomyocytes See reference
Harleton, E. , Besana, A. , Comas, G. , Danilo, P. , Rosen, T. , Argenziano, M. , Rosen, M. , Robinson, R. and Feinmark, S.
Ability to Induce Atrial Fibrillation in the Peri-Operative Period is Associated with Phosphorylation-Dependent Inhibition of TWIK Protein-Related Acid-Sensitive Potassium Channel 1 (TASK-1). ,
J Biol Chem
 288 , 2829-38 , 2013
Canine
Canine 		Atrial and ventricular myocytes Tyrode's solution
Schotten, U. , de Haan, S. , Verheule, S. , Harks, E. , Frechen, D. , Bodewig, E. , Greiser, M. , Ram, R. , Maessen, J. , Kelm, M. , Allessie, M. and Van Wagoner, D.
Blockade of Atrial-Specific K+-currents Increases Atrial but not Ventricular Contractility by Enhancing Reverse Mode Na+/Ca2+-Exchange. ,
Cardiovasc Res
 73 , 37-47 , 2007
Canine
Canine, 20-30 kg 		Cardiomyocytes Tyrode's solution
Sun, H. , Chartier, D. , Leblanc, N. and Nattel, S.
Intracellular Calcium Changes and Tachycardia-Induced Contractile Dysfunction in Canine Atrial Myocytes. ,
Cardiovasc Res
 49 , 751-61 , 2001
Canine
Canine 		Atrial and ventricular myocytes See reference
Zhang, H. , Silva, J. , Lin, Y. , Verbsky, J. , Lee, U. , Kanter, E. , Yamada, K. , Schuessler, R. and Nichols, C.
Heterogeneity and Function of K(ATP) Channels in Canine Hearts. ,
Heart Rhythm
 10 , 1576-83 , 2013
Canine
Dog, mongrel, adult 		Myocytes Joklik's MEM
Spanier, A. and Weglicki, W.
Ca2+-Tolerant Adult Canine Myocytes: Preparation and Response to Anoxia/Acidosis ,
Am J Physiol
 243 , H448 , 1982
Canine
Canine, adult 		Cardiomyocytes M199
Gavi, S. , Yin, D. , Shumay, E. , Wang, H. and Malbon, C.
Insulin-Like Growth Factor-I Provokes Functional Antagonism and Internalization of Beta1-Adrenergic Receptors. ,
Endocrinology
 148 , 2653-62 , 2007
Canine
Canine 		Ventricular and atrial myocytes HEPES/Tyrode's
Gintant, G.
Two Components of Delayed Rectifier Current in Canine Atrium and Ventricle. Does IKs Play a Role in the Reverse Rate Dependence of Class III Agents? ,
Circ Res
 78 , 26-37 , 1996
Canine
Canine, adult 		Cardiomyocytes HEPES
Calloe, K. , Nof, E. , Jespersen, T. , Di Diego, J. , Chlus, N. , Olesen, S.¸ Antzelevitch, C. and Cordeiro, J.
Comparison of the Effects of a Transient Outward Potassium Channel Activator on Currents Recorded from Atrial and Ventricular Cardiomyocytes. ,
J Cardiovasc Electrophysiol
 22 , 1057-66 , 2011
Canine
Canine 		Atrial myocytes HEPES
Baba, S. , Dun, W. , Hirose, M. and Boyden, P.
Sodium Current Function in Adult and Aged Canine Atrial Cells. ,
Am J Physiol Heart Circ Physiol
 291 , H756-61 , 2006
Canine
Canine, adult 		Atrial and ventricular myocytes See reference
Bonilla, I. , Sridhar, A. , Nishijima, Y. , Gyorke, S. , Cardounel, A. and Carnes, C.
Differential Effects of the Peroxynitrite Donor, SIN-1, on Atrial and Ventricular Myocyte Electrophysiology. ,
J Cardiovasc Pharmacol
 61 , 401-7 , 2013
Canine
Canine, 20-25 kg 		Cardiomyocytes Tyrode's solution
Burashnikov, A. , Zygmunt, A. , Di Diego, J. , Linhardt, G. , Carlsson, L. and Antzelevitch, C.
AZD1305 Exerts Atrial Predominant Electrophysiological Actions and is Effective in Suppressing Atrial Fibrillation and Preventing its Reinduction in the Dog. ,
J Cardiovasc Pharmacol
 56 , 80-90 , 2010
Canine
Canine 		Cardiomyocytes Tyrode's solution
Zhang,D. , Wu, C. , Qi, X. , Meijering, R. , Hoogstra-Berends, F. , Tadevosyan, A. and Cubukcuoglu, et al.
Activation of Histone Deacetylase-6 Induces Contractile Dysfunction Through Derailment of alpha-tubulin Proteostasis in Experimental and Human Atrial Fibrillation. ,
Circulation
 129 , 346-58 , 2014
Canine
Canine 		Cardiomyocytes Tyrode's solution
Xi, Y. , Wu, G. , Ai, T. , Cheng, N. , Kalisnik, J. , Sun, J. , Abbasi, S. , Yang, D. , Fan, C. , Yuan, X. and Wang, S. et al.
Ionic Mechanisms Underlying the Effects of Vasoactive Intestinal Polypeptide on Canine Atrial Myocardium. ,
Circ Arrhythm Electrophysiol
 6 , 976-83 , 2013
Chicken
Chick, 10 day 		Ventricular HBSS, CMF
Kim, D. , Okada, A. and Smith, T.W.
Control of Cytosolic Calcium Activity During Low Sodium Exposure in Cultured Chick Heart Cells ,
Circ Res
 61 , 29 , 1987
Chicken
Chick embryos, 5 day 		Heart
Liver 		Tyrode's solution, CMF
Steinberg, M.
"ECM": Its Nature, Origin, And Function in Cell Aggregation ,
Exp Cell Res
 30 , 257 , 1963
Chicken
Chick, 8 day embryo
(also rat, neonate and mouse) 		Myocytes Rinaldini's buffer solution, CF
Gross, W. , Schopf-Ebner, E. and Bucher, O.
Technique for the Preparation of Homogeneous Cultures of Isolated Heart Muscle Cells ,
Exp Cell Res
 53 , 1 , 1968
Chicken
Chick, embryos, 10-11 day 		Cardiomyocytes HBSS, CMF
Wang, S. , Greaser, M.L. , Schultz, E. , Bulinski, J.C. , Lin, J.J. and Lessard, J.L.
Studies on Cardiac Myofibrillogenesis With Antibodies to Titin, Actin, Tropomyosin, and Myosin ,
J Cell Biol
 107 , 1075 , 1988
Chicken
Chick, embryo, 11 day 		Cardiomyocytes HBSS, CMF
Jacob, R. , Lieberman, M. , Murphy, E. and Piwnica-Worms, D.
Effect of Sodium-Potassium Pump Inhibition and Low Sodium on Membrane Potential in Cultured Embryonic Chick Heart Cells ,
J Physiol
 387 , 549 , 1987
Chicken
Hen, White Leghorn 		Heart Medium 199
Dehann, R.
Regulation of Spontaneous Activity and Growth of Embryonic Chick Heart Cells in Tissue Culture ,
Dev Biol
 16 , 216 , 1967
Chicken
Chicken embryos, 9-11 day incubated 		Cardiomyocytes PBS
Eschenhagen, T. , Fink, C. , Remmers, U. , Scholz, H. , Wattchow, J. , Weil, J. and et al.
Three-Dimensional Reconstitution of Embryonic Cardiomyocytes in a Collagen Matrix: A New Heart Muscle Model System ,
FASEB J
 11 , 683 , 1997
Chicken
Chick, embryonic, 11-12-day-old 		Myocytes CMF solution
Murphy, E. , Aiton, J. , Russell, C. and Lieberman, M.
Calcium Elevation in Cultured Heart Cells: Its Role in Cell Injury ,
Am J Physiol Cell Physiol
 245 (14) , C316 , 1983
Chicken
Chicken, embryonic 		Cardiomyocytes HBSS
Blech-Hermoni, Y. and Ladd, A.
Identification of Transcripts Regulated by CUG-BP, Elav-like Family Member 1 (CELF1) in Primary Embryonic Cardiomyocytes by RNA-seq. ,
Genom Data
 6 , 74-76 , 2015
Feline
Cat, mongrel, adult, 1.8-2.8 Kg 		Myocytes
Ventricular 		Kreb's Henseleit, CF
Silver, L. , Hemwall, E. , Marino, T. and Houser, S.
Isolation and Morphology of Calcium-Tolerant Feline Ventricular Myocytes ,
Am J Physiol
 245 , H891 , 1983
Fish
Zebrafish 		Cardiac Myocytes HEPES
Fowler, E. , Wang, N. , Hezzell, M. , Chanoit, G. , Hancox, J. and Cannell, M.
Arrhythmogenic Late Ca2+ Sparks in Failing Heart Cells and Their Control by Action Potential Configuration ,
Proc Natl Acad Sci U S A
 117 , 2687-2692 , 2020
Fish
Fish, Danio rerio 		Cardiomyocytes MEM
Sander, V. , Sune, G. , Jopling, C. , Morera, C. and Belmonte J.
Isolation and In Vitro Culture of Primary Cardiomyocytes from Adult Zebrafish Hearts. ,
Nat Protoc
 8 , 800-9 , 2013
Frog
Frog (Rana esculenta) 		Myocytes CF Ringer
Arrio-Dupont, M. and de Nay, D.
High Yield Preparation of Calcium-Tolerant Myocytes From Frog Ventricles ,
Biol Cell
 54 , 164 , 1985
Guinea-Pig
Guinea-pig, male, 450-600 g 		Myocytes Krebs Henseleit bicarbonate buffer
Stemmer, P. , Akera,T. , Brody, T. , Rardon, D. and Watanabe, A.
Isolation and Enrichment of Ca2+-Tolerant Myocytes for Biochemical Experiments from Guinea-Pig Heart ,
Life Sci
 44 , 1231 , 1989
Guinea-Pig
Guinea-pig 		Myocytes Bicarbonate buffer, CF
Bridge, J. , Spitzer, K. and Ershler, P.
Relaxation of Isolated Ventricular Cardiomyocytes by a Voltage-Dependent ,
Science
 241 , 823 , 1988
Guinea-Pig
Guinea-pig 		Cardiomyocytes Tyrode solution, CF
Ishihara, K. , Mitsuiye, T. , Noma, A. and Takano, M.
The Mg2+ Block and Intrinsic Gating Underlying Inward Rectification of the K+ Current in Guinea-Pig Cardiac Myocytes ,
J Physiol
 419 , 297 , 1989
Guinea-Pig
Guinea pig, 250-450 g 		Endothelial Perfusing solution
(see reference)
Preisig-Muller, R. , Mederos Y Schnitzler, M. , Derst, C. and Daut J.
Separation of Cardiomyocytes and Coronary Endothelial Cells for Cell-Specific RT-PCR ,
Am J Physiol
 277 , H413 , 1999
Guinea-Pig
Guinea pig, adult 		Cardiomyocytes M-199
Zorn-Pauly, K. , Schaffer, P. , Pelzmann, B. , Bernhart, E. , Lang, P. and Koidl, B.
L-type and T-type Ca2+ current in cultured ventricular guinea pig myocytes ,
Physiol Res
 53(4) , 369 , 2004
Guinea-Pig
Guinea pig, female, 300-350g 		Coronary endothelial see reference
Buxton I L, Kaiser R A, Oxhorn B C, Cheek D J.
Evidence supporting the Nucleotide Axis Hypothesis: ATP release and metabolism by coronary endothelium ,
Am J Physiol Heart Circ Physiol
 281 , H1657-66 , 2001
Guinea-Pig
Guinea-Pig 		Cardiomyocytes See reference
Dhamoon, A, Pandit, S. , Sarmast, F. , Parisian, K. , Guha, P. , Li, Y. , Bagwe, S. , Taffet, S. and Anumonwo, J.
Unique Kir2.x Properties Determine Regional and Species Differences in the Cardiac Inward Rectifier K+ Current. ,
Circ Res
 94 , 1332-9 , 2004
Guinea-Pig
Guinea pig, male, 350-400g 		Ventricular myocytes Tyrodes solution
Wang, Y. , Geng, J. , Jiang, M. , Li, C. , Han, Y. and Jiang, J.
The Cardiac Electrophysiology Effects of Higenamine in Guinea Pig Heart ,
Biomed Pharmacother
 109 , 2348 , 2019
Human
Human 		Cardiomyocytes HEPES solution
Todor Anastassia, Sharov Victor G, Tanhehco Elaine J, Silverman Norman, Bernabei Alvise, Sabbah Hani N.
Hypoxia-induced cleavage of caspase-3 and DFF45/ICAD in human failed cardiomyocytes ,
Am J Physiol Heart Circ Physiol
 283 , H990-5 , 2002
Human
Human 		Coronary artery smooth muscle DMEM
Jensen, B. , Swigart, P. , Laden, M. , DeMarco, T. , Hoopes, C. and Simpson, P.
The Alpha-1D Is the Predominant Alpha-1-Adrenergic Receptor Subtype in Human Epicardial Coronary Arteries. ,
J Am Coll Cardiol
 54 , 1137 , 2009
Human
Human 		Smooth muscle aortic DMEM
Mathew, S. , Tustison, K. , Sugatani, T. , Chaudhary, L. , Rifas, L. and Hruska, K.
The Mechanism of Phosphorus as a Cardiovascular Risk Factor in CKD. ,
J Am Soc Nephrol
 19 , 1092 , 2008
Human
Human 		Atrial cardiomyocytes see reference
Voigt, N. , Makary, S. , Nattel, S. and Dobrev, D.
Voltage-Clamp-Based Methods for the Detection of Constitutively Active Acetylcholine-Gated I(K,ACh) Channels in the Diseased Heart. ,
Methods Enzymol
 484 , 653 , 2010
Human
Human 		Myofibers see reference
Anderson, E. , Rodriguez, E. , Anderson, C. , Thayne, K. , Chitwood, W. and Kypson, A.
Increased Propensity for Cell Death in Diabetic Human Heart is Mediated by Mitochondrial-Dependent Pathways. ,
Am J Physiol Heart Circ Physiol
 300 , H118 , 2011
Human
Human 		Atrial myocytes see references
Voigt, N. , Zhou, X. and Dobrev, D.
Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents. ,
J Vis Exp
 , e50235 , 2013
Human
Human 		Atrial myocytes see reference
Van Wagoner, D. , Pond, A. , McCarthy, P. , Trimmer, J. and Nerbonne, J.
Outward K+ Current Densities and Kv1.5 Expression are Reduced in Chronic Human Atrial Fibrillation. ,
Circ Res
 80 , 772-81 , 1997
Human
Human 		Cardiac DPBS
Holt-Casper, D. , Theisen, J. , Moreno, A. , Warren, M. , Silva, F. , Grainger, D. , Bull, D. and Patel, A.
Novel Xeno-free Human Heart Matrix-Derived Three-Dimensional Scaffolds. ,
J Transl Med
 13 , 194 , 2015
Human
Human 		Cardiac stem Joklik modified Eagle’s
Avolio, E. , Gianfranceschi, G. , Cesselli, D. , Caragnano, A. , Athanasakis, E. , Katare, R. , Meloni, M. , Palma, A. , Barchiesi, A. , Vascotto, C. , Toffoletto, B. , Mazzega, E. , Finato, N. , Aresu, G. and Livi, U.
Ex vivo Molecular Rejuvenation Improves the Therapeutic Activity of Senescent Human Cardiac Stem Cells in a Mouse Model of Myocardial Infarction. ,
Stem Cells
 32 , 2373 , 2014
Human
Human 		Cardiac DMEM
Vukusic, K. , Jonsson, M. , Brantsing, C. , Dellgren, G. , Jeppsson, A. , Lindahl, A. and Asp, J.
High Density Sphere Culture of Adult Cardiac Cells Increases the Levels of Cardiac and Progenitor Markers and Shows Signs of Vasculogenesis. ,
Biomed Res Int
 2013 , 2013
Human
Human, both sexes, 18-81 years 		Myocardial
Atrial 		HBSS, CMF
Smith, D. , Glover, J. , Townsend, L. and Maupin, E.
A Method for the Harvest, Culture, and Characterization of Human Adult Atrial Myocardial Cells: Correlation with Age of Donor ,
In Vitro Cell Dev Biol
 27 , 914 , 1991
Human
Human 		Cardiomyocytes and endothelial PBS
Shirai, T. , Rao, V. , Weisel, R. , Ikonomidis, J. , Li, R. , Tumiati, L. , Merante, F. and Mickle, D.
Preconditioning Human Cardiomyocytes and Endothelial Cells. ,
J Thorac Cardiovasc Surg
 115 , 210-9 , 1998
Human
Human, fetal, 14.5 week gestation 		Myocytes HBSS with Calcium
Goldman, B. and Wurzel, J.
Effects of Subcultivation and Culture Medium on Differentiation of Human Fetal Cardiac Myocytes ,
In Vitro Cell Dev Biol
 28 , 109 , 1992
Human
Human 		Cardiospheres Iscove modified DMEM
Sharma, S. , Mishra, R. , Simpson, D. , Wehman, B. , Colletti, E. , Deshmukh, S. , Datla, S. , Balachandran, K. , Guo, Y. , Chen, L. , Siddiqui, O. , Kaushal, S. and Kaushal, S.
Cardiosphere-Derived Cells From Pediatric End-Stage Heart Failure Patients Have Enhanced Functional Activity Due to the Heat Shock Response Regulating the Secretome. ,
Stem Cells
 33 , 1213-29 , 2015
Human
Human, adult 		Myocytes Joklik's MEM
Bugaisky, L.B.
Biology of Isolated Adult Cardiac Myocytes , Isolation and Culture of Human Adult Cardiac Myocytes , (Clark,Decker,Borg,eds) , Elsevier Science Publishing Co., Inc. , 1988
Human
Human 		Thoracic aorta Phosphate buffer w/NaCl
Hassler, O. , Wiren, M. and Herbertsson, S.
The Elastic Coat of the Arterial Wall Studied with the Aid of Collagenase ,
Acta Pathol Microbiol Scand
 57 , 15 , 1963
Human
Human 		Heart
Myocytes 		Tyrode's solution
Hoppe, U. , Jansen, E. , Sudkamp, M. and Beukelmann, D.
Hyperpolarization-Activated Inward Current in Ventricular Myocytes From Normal and Failing Human Hearts ,
Circulation
 97 , 55 , 1998
Human
Human (n=16, age: 60 &plusmin;3 years) 		Myocardial Medium 199
Mukerjee, R. , Multani, M. , Sample, J. , Dowdy, K. , Zellner, J. , Hoover, D. and Spinale, F.
Effects of adrenomedullin on human myocyte contractile function and beta-adrenergic response ,
J Cardiovasc Pharmacol
 7 (4) , 235 , 2002
Invertebrate
Octopus, E. cirhosa, 260-352 g 		Systemic heart cardiomyocytes see reference
Altimiras J. , Hove-Madsen L. and Gesser H.
Ca(2+) Uptake in the Sarcoplasmic Reticulum from the Systemic Heart of Octopod Cephalopods ,
J Exp Biol
 202 , 2531 , 1999
Mouse
Mouse 		Aortic endothelial DMEM
Kobayashi, M. , Inoue, K. , Warabi, E. , Minami, T. and Kodama, T.
A Simple Method of Isolating Mouse Aortic Endothelial Cells. ,
J Atheroscler Thromb
 12 , 138 , 2005
Mouse
Mouse (BALB/c), adult 		Myocytes Tyrode's solution
Felzen, B. , Shilkrut, M. , Less, H. , Sarapov, I. , Maor, G. , Coleman, R. , Robinson, R. and et al.
Fas (CD95/Apo-1)-Mediated Damage to Ventricular Myocytes Induced by Cytotoxic T Lymphocytes from Perforin-Deficient Mice ,
Circ Res
 82 , 438 , 1998
Mouse
Mouse 		Cardiomyocytes Joklik's MEM
Christensen G, Minamisawa S, Gruber PJ, Wang Y, Chien KR.
High-efficiency, long-term cardiac expression of foreign genes in living mouse embryos and neonates ,
Circulation
 101 , 178-84 , 2000
Mouse
Mouse, 3-4 week 		Cardiac progenitor DMEM
Wang, H. , Chen, H. , Feng, B. , Wang, X. , He, X. , Hu, R. , Yin, M. , Wang, W. , Fu, W. and Xu, Z.
Isolation and Characterization of a Sca-1+/CD31- Progenitor Cell Lineage Derived From Mouse Heart Tissue. ,
BMC Biotechnol
 14 , 75 , 2014
Mouse
Mouse 		Cardiomyocyte PBS
Soonpaa, M. , Kim, K. , Pajak, aL. , Franklin, M. and Field, L.
Cardiomyocyte DNA Synthesis and Binucleation During Murine Development ,
Am J Physiol
 271 , H2183 , 1996
Mouse
Mouse, female, 14-18 g 		Cardiomyocytes see reference
Zhang Sui, Wang Dachun, Estrov Zeev, Raj Sean, Willerson James T, Yeh Edward T H.
Both cell fusion and transdifferentiation account for the transformation of human peripheral blood CD34-positive cells into cardiomyocytes in vivo ,
Circulation
 110 , 3803-7 , 2004
Mouse
Mouse 		Cardiomyocytes L-15
Touchberry, C. , Green, T. , Tchikrizov, V. , Mannix, J. , Mao, T. , Carney, B. , Girgis, M. , Vincent, R. , Wetmore, L. , Dawn, B. , Bonewald, L. , Stubbs, J. and Wacker, M.
FGF23 is a Novel Regulator of Intracellular Calcium and Cardiac Contractility in Addition to Cardiac Hypertrophy. ,
Am J Physiol Endocrinol Metab
 304 , E863 , 2013
Mouse
Mouse, 6-8 week 		Vascular smooth muscle EBSS
Qian, Q. , Hunter, L. , Li, M. , Marin-Padilla, M. , Prakash, Y. , Somlo, S. , Harris, P. , Torres, V. and Sieck, G.
Pkd2 Haploinsufficiency Alters Intracellular Calcium Regulation in Vascular Smooth Muscle Cells ,
Hum Mol Genet
 12(15) , 1875 , 2003
Mouse
Mouse 		Ventricular myocytes and mesenteric arterial SMC Krebs-Ringer
Lu Tong, Ye Dan, Wang Xiaoli, Seubert John M, Graves Joan P, Bradbury J Alyce, Zeldin Darryl C, Lee Hon-Chi.
Cardiac and vascular KATP channels in rats are activated by endogenous epoxyeicosatrienoic acids through different mechanisms ,
J Physiol
 575 , 627-44 , 2006
Mouse
Mouse, adult 		Cardiac Progenitor MEM
Santos Nascimento, D, Mosqueira, D, Sousa, L, Teixeira, M, Filipe, M, Resende, T, Araujo, A, Valente, M, Almeida, J, Martins, J, Santos, J, Barcia, R, Cruz, P, Cruz, H. and Pinto-d-O, P
Human Umbilical Cord Tissue-Derived Mesenchymal Stromal Cells Attenuate Remodeling after Myocardial Infarction by Proangiogenic, Antiapoptotic, and Endogenous Cell-Activation Mechanisms. ,
Stem Cell Res Ther
 5 , 5 , 2014
Mouse
Mouse 		Cardiomyocytes DMEM/F-12
Rybkin, I. , Markham, D. , Yan, Z. , Bassel-Duby, R. , Williams, R. and Olson, E.
Conditional Expression of SV40 T-antigen in Mouse Cardiomyocytes Facilitates an Inducible Switch from Proliferation to Differentiation ,
J Biol Chem
 278(18) , 15927 , 2003
Mouse
Mouse, adult 		Cardiomyocytes HBSS
O'Connell Timothy D, Rodrigo Manoj C, Simpson Paul C.
Isolation and culture of adult mouse cardiac myocytes ,
Methods Mol Biol
 357 , 271-96 , 2007
Mouse
Mouse, 15-30 gm 		Ventricular myocytes see reference
Henn, M. , Janjua, M. , Kanter, E. , Makepeace, C. , Schuessler, R. , Nichols, C. and Lawton, J.
Adenosine Triphosphate-Sensitive Potassium Channel Kir Subunits Implicated in Cardioprotection by Diazoxide. ,
J Am Heart Assoc
 4 , 2015
Mouse
Mouse, neonatal and adult 		Cardiomyocytes Joklik's MEM
Schreiber, K. , Paquet, L. , Allen, B. and Rindt, H.
Protein Kinase C Isoform Expression and Activity in the Mouse Heart ,
Am J Physiol Heart Circ Physiol
 281(5) , H2062 , 2001
Mouse
Mouse 		Smooth muscle cells F10 Ham
Verheye, S. , Martinet, W. , Kockx, M. , Knaapen, M. , Salu, K. , Timmermans, J. , Ellis, J. , Kilpatrick, D. and DeMayer, G.
Selective Clearance of Macrophages in Atherosclerotic Plaques by Autophagy ,
J Am Coll Cardiol
 49 , 706 , 2007
Mouse
Mouse 		Cardiac progenitor HBSS
Zafiriou, M. , Noack, C. , Unsold, B. , Didie, M. , Pavlova, E. , Fischer, H. , Reichardt, H. , Bergmann, M. , El-Armouche, A. , Zimmermann, W. and Zelarayan, L.
Erythropoietin Responsive Cardiomyogenic Cells Contribute to Heart Repair Post Myocardial Infarction. ,
Stem Cells
 32 , 2480 , 2014
Mouse
Mouse, 6-9 or 21-41 day 		Ventricular and atrial myocytes DMEM/Tyrodes
Valenzuela, D. , Han, X. , Mende, U. , Fankhauser, C. , Mashimo, H. , Huang, P. , Pfeffer, J. , Neer, E. and Fishman, M.
G alpha(o) is Necessary for Muscarinic Regulation of Ca2+ Channels in Mouse Heart ,
Proc Natl Acad Sci U S A
 94(5) , 1727 , 1997
Mouse
Mouse 		Cardiomyocytes PBS
Chen, H. , Yong, W. , Ren, S. , Shen, W. , He, Y. , Cox, K. , Zhu, W. , Li, W. , Soonpaa, M. , Payne, RM, Franco, D. , Field, L. , Rosen, V. , Wang, Y. and Shou, W.
Overexpression of Bone Morphogenetic Protein 10 in Myocardium Disrupts Cardiac Postnatal Hypertrophic Growth. ,
J Biol Chem
 281 , 27481 , 2006
Mouse
Mouse, male, 12-18 week 		Cardiomyocytes Perfusion solution (see reference)
Jian, Z, Chen, Y, Shimkunas, R, Jian, Y, Jaradeh, M, Chavez, K, Chiamvimonvat, N, Tardiff, J, Izu, L, Ross, R. and Chen-Izu, Y
In Vivo Cannulation Methods for Cardiomyocytes Isolation from Heart Disease Models. ,
PLoS ONE
 11 , e0160605 , 2016
Mouse
Mouse, 1-3 day 		Cardiomyocytes FBS-MEM
Wang, G. and Kang, Y.
Inhibition of Doxorubicin Toxicity in Cultured Neonatal Mouse Cardiomyocytes with Elevated Metallothionein Levels ,
J Pharmacol Exp Ther
 288(3) , 938 , 1999
Mouse
Mouse, adult 		Ventricular myoctes M199
Zhang, Y, Kanter, E. , Laing, J. , Aprhys, C. , Johns, D. , Kardami, E. and Yamada, K.
Connexin43 Expression Levels Influence Intercellular Coupling and Cell Proliferation of Native Murine Cardiac Fibroblasts. ,
Cell Commun Adhes
 15 , 289 , 2008
Mouse
Mouse 		Aortic DPBS
Hu, D. , Yin, C. , Mohanta, S. , Weber, C. and Habenicht, A.
Prepartaion of Single Cell Suspensions from Mouse Aorta ,
Bio Protoc
 6 , 11 , 2016
Mouse
Mouse, neonatal 2-4 day 		Atrial myocytes
  • NCIS kit : with modifications see reference
L-15
Bettahi, I,, Marker, C. , Roman, M. and Wickman, K.
Contribution of the Kir3.1 Subunit to the Muscarinic-gated Atrial Potassium Channel IKACh ,
J Biol Chem
 277 , 48282 , 2002
Mouse
Mouse, male, 6 week 		Coronary endothelial cells M199
Makino, A. , Suarez, J. , Wang, H. , Belke, D. , Scott, B. and Dillmann, W.
Thyroid Hormone Receptor-beta is Associated With Coronary Angiogenesis During Pathological Cardiac Hypertrophy. ,
Endocrinology
 150 , 2008 , 2009
Mouse
Mouse 		Cardiac endothelial PBS
Pratumvinit, B. , Reesukumal, K. , Janebodin, K. , Ieronimakis, N. and Reyes, M.
Isolation, Characterization, and Transplantation of Cardiac Endothelial Cells. ,
Biomed Res Int
 2013 , 359412 , 2013
Mouse
Mouse, 1 day 		Cardiomyocytes
  • NCIS kit : with modifications see reference
L-15
Lader, A. , Kwiatkowski, D. and Cantiello, H.
Role of Gelsolin in the Actin Filament Regulation of Cardiac L-type Calcium Channels ,
Am J Physiol
 277 , C1277 , 1999
Mouse
Mouse, male, 8-25 week 		Cardiomyocytes MEM
Carley, A. and Kleinfeld, A.
Fatty Acid (FFA) Transport in Cardiomyocytes Revealed by Imaging Unbound FFA is Mediated by an FFA Pump Modulated by the CD36 Protein. ,
J Biol Chem
 286 , 4589-97 , 2011
Mouse
Mouse, adult 		Cardiomyocytes Joklik's MEM
Grupp, I. , Lorenz, J. , Walsh, R. , Boivin, G. and Rindt, H.
Overexpression of Alpha1B-Adrenergic Receptor Induces Left Ventricular Dysfunction in the Absence of Hypertrophy. ,
Am J Physiol
 275 , H1338-50 , 1998
Mouse
Mouse, 1 day 		Cardiomyocytes
  • NCIS kit : with modifications see reference
L-15
Lader, A. , Xiao, Y. , Ishikawa, Y. , Cui, Y. , Vatner, D. , Vatner, S. , Homcy, C. and Cantiello, H.
Cardiac Gsalpha Overexpression Enhances L-type Calcium Channels Through an Adenylyl Cyclase Independent Pathway ,
Proc Natl Acad Sci U S A
 95 , 9669 , 1998
Mouse
Mouse 		Aortic adventitial leukocytes PBS
Butcher, M. , Herre, M. , Ley, K. and Galkina, E.
Flow Cytometry Analysis of Immune Cells Within Murine Aortas. ,
J Vis Exp
 53 , e2828 , 2011
Mouse
Mouse, 4-6 week 		Cardiospheres PBS
Xu, J. , Lee, Y. , Ran, X. , Liao, S. , Yang, J. , Au, K. , Lai, W. , Esteban, M. and Tse, H.
Generation of Induced Cardiospheres via Reprogramming of Skin Fibroblasts for Myocardial Regeneration. ,
Stem Cells
 34 , 2693-2706 , 2016
Mouse
Mouse, neonatal 		Cardiomyoctes M199
Takahashi N, Wang X, Tanabe S, Uramoto H, Jishage K, Uchida S, Sasaki S, Okada Y.
ClC-3-independent sensitivity of apoptosis to Cl- channel blockers in mouse cardiomyocytes ,
Cell Physiol Biochem
 15 , 263 , 2005
Mouse
Mouse, adult 		Cardiomyocytes see reference
Flynn, J. , Santana, L. and Melov, S.
Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes. ,
J Vis Exp
 58 , e3302 , 2011
Mouse

Mouse, 8-12 week

 Cardiomyocytes Perfusion buffer (see reference)
Ackers-Johnson, M. , Li, P. , Holmes, A. , O'Brien, S. , Pavlovic, D. and Foo, R.
A Simplified, Langendorff-Free Method for Concomitant Isolation of Viable Cardiac Myocytes and Nonmyocytes From the Adult Mouse Heart. ,
Circ Res
 119 , 909-20 , 2016
Mouse
Mouse, 1-6 day 		Cardiomyocytes PBS
Watzka SB, Lucien J, Shimada M, Edwards V, Yeger H, Hannigan G. and Coles JG.
Selection of viable cardiomyocytes for cell transplantation using three-dimensional tissue culture ,
Transplantation
 70 , 1310 , 2000
Mouse
Mouse, adult 		Cardiomyocytes see reference
Kohncke, C. Lisewski, U. , Schleussner, L. , Gaertner, C. , Reichert, S. and Roepke, T.
Isolation and Kv Channel Recordings in Murine Atrial and Ventricular Cardiomyocytes. ,
J Vis Exp
 73 , e50145 , 2013
Mouse
Mouse
(also rat, neonate and chick, 8 day embryo) 		Myocytes Rinaldini's buffer solution, CF
Gross, W. , Schopf-Ebner, E. and Bucher, O.
Technique for the Preparation of Homogeneous Cultures of Isolated Heart Muscle Cells ,
Exp Cell Res
 53 , 1 , 1968
Mouse
Mouse 		Smooth muscle cells DMEM
Fukumoto Yoshihiro, Deguchi Jun-o, Libby Peter, Rabkin-Aikawa Elena, Sakata Yasuhiko, Chin Michael T, Hill Christopher C, Lawler Patrick R, Varo Nerea, Schoen Frederick J, Krane Stephen M, Aikawa Masanori.
Genetically determined resistance to collagenase action augments interstitial collagen accumulation in atherosclerotic plaques ,
Circulation
 110 , 1953-9 , 2004
Mouse
Mouse 		Cardiomyocytes MEM
Hennessey, J. , Wei, E. and Pitt, G.
Fibroblast Growth Factor Homologous Factors Modulate Cardiac Calcium Channels. ,
Circ Res
 113 , 381-8 , 2013
Mouse
Mouse embryos 		Myocytes DMEM
Wobus, A. , Kleppisch, T. , Maltsev, V. and Hescheler, J.
Cardiomyocyte-Like Cells Differentiated In Vitro From Embryonic Carcinoma Cells P19 are Characterized by Functional Expression of Adrenoceptors and Ca2+ Channels ,
In Vitro Cell Dev Biol
 30A , 425 , 1994
Mouse

Mouse, adult

 Cardiomyocytes Tyrode solution
Zhang, Z. , Zheng, W. , He, D. , Hu, Z. , Xie, Q. , Huang, M. , Li, W. and Huang, Z.
An Improved Procedure for Isolating Adult Mouse Cardiomyocytes for Epicardial Activation Mapping. ,
J Cell Mol Med
 25 , 11257-11263 , 2021
Mouse
Mouse, 2-4 mo 		Ventricular myocytes MEM
Zhou YY, Wang SQ, Zhu WZ, Chruscinski A, Kobilka BK, Ziman B, Wang S, Lakatta EG, Cheng H, Xiao RP.
Culture and adenoviral infection of adult mouse cardiac myocytes: methods for cellular genetic physiology ,
Am J Physiol Heart Circ Physiol
 279 , H429-36 , 2000
Mouse
Mouse, adult, 6-12 week 		Cardiomyocytes see reference
Shioya, T.
A Simple Technique for Isolating Healthy Heart Cells from Mouse Models. ,
J Physiol Sci
 57 , 327-35 , 2007
Mouse
Mouse, NTg and Tg, 8 - 12 week 		Myocytes Joklik's MEM
Nelson, D. , Setser, E. , Hall, D. , Schwartz, S. , Hewitt, T. , Klevitsky, R. , Osinska, H. , Bellgrau, D. , Duke, R. and Robbins, J.
Proinflammatory Consequences of Transgenic Fas Ligand Expression in the Heart ,
J Clin Invest
 105 (9) , 1199 , 2000
Mouse

Mouse, neonatal

 Cardiomyocytes L-15
Angelini, A. , Pi, X. and Xie, L.
Evaluation of Long-Chain Fatty Acid Respiration in Neonatal Mouse Cardiomyocytes using SeaHorse Instrument. ,
Star Protocols
 3 , 101392 , 2022
Mouse
Mouse, 3-7 month 		Fibroblasts, mesangial, smooth muscle DMEM
Bradshaw AD, Francki A, Motamed K, Howe C, Sage EH.
Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation ,
Mol Biol Cell
 10 , 1569-79 , 1999
Mouse
Mouse, adult, 9-13 week 		Cardiomyocytes Myocyte buffer see reference
Kabaeva, Z. , Zhao, M. and Michele, D.
Blebbistatin Extends Culture Life of Adult Mouse Cardiac Myocytes and Allows Efficient and Stable Transgene Expression. ,
Am J Physiol Heart Circ Physiol
 294 , H1667-74 , 2008
Mouse
Mouse, P0-P1 		Cardiomyocytes DMEM, Medium 199
Arber, S. , Hunter, J. , Ross, J. , Hongo, M. , Sansig, G. , Borg, J. , Perriard, J. , Chien, K. and Caroni, P.
MLP-Deficient Mice Exhibit a Disruption of Cardiac Cytoarchitectural Organization, Dilated Cadiomyopathy, and Heart Failure ,
Cell
 88 , 393 , 1997
Mouse
Mouse, 1 day 		Cardiomyoctes EBSS
Potts, Malia B. , Vaughn, Allyson E. , McDonough, Holly, Patterson, Cam. and Deshmukh, Mohanish
Reduced Apaf-1 levels in cardiomyocytes engage strict regulation of apoptosis by endogenous XIAP ,
J Cell Biol
 171 , 925 , 2005
Mouse
Mouse, 2-6 month 		Sinoatrial node Tyrode's solution
Christel, C. , Cardona, N. , Mesirca, P. , Herrmann, S. , Hofmann, F. , Striessnig, J. , Ludwig, A. , Mangoni, M. and Lee, A.
Distinct Localization and Modulation of Cav1.2 and Cav1.3 L-type Ca2+ Channels in Mouse Sinoatrial Node. ,
J Physiol
 590 , 6327 , 2012
Ovine
Ovine 		Cardiomyocytes See reference
Dibb, K. , Clarke, J. , Horn, M. , Richards, M. , Graham, H. , Eisner, D. and Trafford, A.
Characterization of an Extensive Transverse Tubular Network in Sheep Atrial Myocytes and its Depletion in Heart Failure. ,
Circ Heart Fail
 2 , 482-9 , 2009
Ovine
Ovine 		Cardiomyocytes See reference
Dhamoon, A, Pandit, S. , Sarmast, F. , Parisian, K. , Guha, P. , Li, Y. , Bagwe, S. , Taffet, S. and Anumonwo, J.
Unique Kir2.x Properties Determine Regional and Species Differences in the Cardiac Inward Rectifier K+ Current. ,
Circ Res
 94 , 1332-9 , 2004
Ovine
Ovine 		Atrial myocytes Tyrode's solution
Voigt, N. , Pearman, C. , Dobrev, D. and Dibb, K.
Methods for Isolating Atrial Cells from Large Mammals and Humans. ,
J Mol Cell Cardiol
 86 , 187-98 , 2015
Porcine
Porcine 		Coronary vascular smooth muscle (PCVSMCs) HEPES
Christ, M. , Gunther, A. , Heck, M. , Schmidt, B. , Falkenstein, E. and Wehling, M.
Aldosterone, Not Estradiol, Is the Physiological Agonist for Rapid Increases in cAMP in Vascular Smooth Muscle Cells ,
Circulation
 99 , 1485 , 1999
Porcine
Miniature swine, adult, female, 25-40 kg, familiarized with treadmill exercise 		Coronary smooth muscle Krebs bicarbonate solution
Bowles, D. , Hu, Q. , Laughlin, M. and Sturek, M.
Exercise Training Increases L-type Calcium Current Density in Coronary Smooth Muscle ,
Am J Physiol
 275 (44) , H2159 , 1998
Porcine
Porcine 		Aortic smooth muscle and intersitial cells PBS
Butcher, J. and Nerem, R.
Porcine Aortic Valve Interstitial Cells in Three-Dimensional Culture: Comparison of Phenotype with Aortic Smooth Muscle Cells. ,
J Heart Valve Dis
 13 , 478 , 2004
Rabbit
Rabbit, adult, 2 kg 		Myocytes Kreb's Ringer
Dani, A. , Cittadini, A. , Flamini, G. , Festuccia, G. and Terranova, T.
Preparation and Some Properties of Isolated Beating Myocytes from Adult Rabbit Heart ,
J Mol Cell Cardiol
 9 , 777 , 1977
Rabbit
Rabbit, New Zealand, white, male, 2-3 kg 		Cardiomyocytes Eagle's MEM
Buxton, I. and Brunton, L.
Compartments of Cyclic AMP and Protein Kinase in Mammalian Cardiomyocytes ,
J Biol Chem
 258 (17) , 10233 , 1983
Rabbit
Rabbit, adult 1.5-2.5 kg 		Tricuspid valve cells HEPES Tyrode solution
Anumonwo JM, Delmar M. and Jalife J.
Electrophysiology of single heart cells from the rabbit tricuspid valve ,
J Physiol
 425 , 145 , 1990
Rabbit
Rabbit 		Smooth muscle cells F10 Ham
Verheye, S. , Martinet, W. , Kockx, M. , Knaapen, M. , Salu, K. , Timmermans, J. , Ellis, J. , Kilpatrick, D. and DeMayer, G.
Selective Clearance of Macrophages in Atherosclerotic Plaques by Autophagy ,
J Am Coll Cardiol
 49 , 706 , 2007
Rabbit
Rabbit, New Zealand, 2 kg 		Cardiomyocytes and fibroblasts Medium 199
Driesen Ronald B, Dispersyn Gerrit D, Verheyen Fons K, van den Eijnde Stefan M, Hofstra Leo, Thone Fred, Dijkstra Petra, Debie Wiel, Borgers Marcel, Ramaekers Frans C S.
Partial cell fusion: a newly recognized type of communication between dedifferentiating cardiomyocytes and fibroblasts ,
Cardiovasc Res
 68 , 37-46 , 2005
Rabbit
Rabbit, New Zealand, 2.3-2.9 kg 		Cardiomyocytes Krebs-Henseleit
Farkas, A. , Acsai, K. , Nagy, N. , Toth, A. , Fulop, F. , Seprenyi, G. , Birinyi, P. , Nanasi, P. , Forster, T. , Csanady, M. , Papp, J. , Varrro, A. and Farkas, A.
Na(+)/Ca(2+) Exchanger Inhibition Exerts a Positive Inotropic Effect in the Rat Heart, but Fails to Influence the Contractility of the Rabbit Heart. ,
Br J Pharmacol
 154 , 93 , 2008
Rabbit
Rabbit, New Zealand, 3-3.5 kg 		Cardiac Myocytes HEPES
Fowler, E. , Wang, N. , Hezzell, M. , Chanoit, G. , Hancox, J. and Cannell, M.
Arrhythmogenic Late Ca2+ Sparks in Failing Heart Cells and Their Control by Action Potential Configuration ,
Proc Natl Acad Sci U S A
 117 , 2687-2692 , 2020
Rabbit
Rabbit, New Zealand White, 2-3 kg 		Myocytes HEPES
Spitzer, K. , Ershler, P. , Skolnick, R. and Vaughan-Jones, R.
Generation of Intracellular pH Gradients in Single Cardiac Myocytes with a Microperfusion System ,
Am J Physiol
 278 , H1371 , 2000
Rabbit
Rabbit, New Zealand, white, either sex 		Myocytes EGTA-KB
Sedarat, F. , Xu, L. , Moore, E. and Tibbits, G.
Colocalization of Dihydropyridine and Ryanodine Receptors in Neonate Rabbit Heart Using Confocal Microscopy ,
Am J Physiol Heart Circ Physiol
 279 , H202 , 2000
Rat
Rats, AW, late-gestation pregnant females 		Ventricular cardiomyocytes See reference
Engelmann, G. , McTiernan, C. , Gerrity, R. and Samarel, A.
Serum-free Primary Cultures of Neonatal Rat Cardiomyocytes: Cellular and Molecular Applications ,
Technique
 2 (6) , 279 , 1990
Rat
Rat, Wistar, 175-200g 		Cardiomyocytes see reference
Eckerle, L. , Felix, S. and Herda, L.
Measurement of Antibody Effects on Cellular Function of Isolated Cardiomyocytes. ,
J Vis Exp
 73 , e4237 , 2013
Rat
Rat, neonatal 		Cardiomyocytes L-15
Li TS, Ito H, Kajiwara K. and Hamano K.
Long-term survival of xenografted neonatal cardiomyocytes by adenovirus-mediated CTLA4-Ig expression and CD40 blockade ,
Circulation
 108 , 1760 , 2003
Rat
Rat 		Ventricular fibroblasts ADS Buffer:see reference
Jenkins, C. , Milsted, A. , Doane, K. , Meszaros, G. , Toot, J. and Ely, D.
A Cell Culture Model using Rat Coronary Artery Adventitial Fibroblasts to Measure Collagen Production. ,
BMC Cardiovasc Disord
 7 , 13 , 2007
Rat
Rat, neonatal, 1-4 days 		Cardiomyocytes Krebs-Henseleit Buffer, CF
Marino, T.A. , Walter, R.,Cobb, E. , Palasiuk, M. , Parsons, T. and Mercer, W.E.
Effects of Norepinephrine on Neonatal Rat Cardiocyte Growth and Differentiation ,
In Vitro Cell Dev Biol
 26 , 229 , 1990
Rat
Rat, SD, female, 200g 		Ventricular cardiac myocytes Krebs-Henseleit buffer
Westfall M V, Rust E M, Metzger J M.
Slow skeletal troponin I gene transfer, expression, and myofilament incorporation enhances adult cardiac myocyte contractile function ,
Proc Natl Acad Sci U S A
 94 , 5444-9 , 1997
Rat
Rat, male, 200-300 g 		Myocytes Kreb's Henseleit buffer
Farmer, B. , Harris, R. , Jolly, W. , Hathaway, D. , Katzberg, A. , Watanabe, A. , Whitlow, A. and Besch, H.
Isolation and Characterization of Adult Rat Heart Cells ,
Arch Biochem Biophys
 179 , 545 , 1977
Rat
Rat, neonatal 		Cardiomyocytes L-15
Robinet, A. , Millart, H. , Oszust, F. , Hornebeck, W. and Bellon, G.
Binding of Elastin Peptides to S-Gal Protects the Heart Against Ischemia/Reperfusion Injury by Triggering the RISK Pathway. ,
FASEB J
 21 , 1968 , 2007
Rat
Rat, SD, pregnant female 		Peritoneal mast DMEM (see reference)
DeAlmeida, A. , Mustin, D. , Forman, M. , Brower, G. , Janicki, J. and Carver, W.
Effects of mast cells on the behavior of isolated heart fibroblasts: modulation of collagen remodeling and gene expression ,
J Cell Physiol
 191 , 51 , 2002
Rat
Rat, adult 		Ventricular myocytes Medium 199
Karakikes, I. , Hadri, L. , Rapti, K. , Ladage, D. , Ishikawa, K. , Tilemann, L. , Yi, G. , Morel, C. , Gwathmey, J. , Zsebo, K. , Weber, T. , Kawase, Y. and Hajjar, R.
Concomitant Intravenous Nitroglycerin with Intracoronary Delivery of AAV1.SERCA2a Enhances Gene Transfer in Porcine Hearts. ,
Mol Ther
 20 , 565 , 2012
Rat
Rat, SD, 1-2 day 		Cardiomyocytes L-15
Muller-Bore, B. , Cascio, W. , Anderson, P. , Snowwaert, J. , Frye, J. , Desai, N. , Esch, G. , Brackham, J. , Bagnell, C. , Coleman, W. , Grisham, J. and Malouf, N.
Adult-derived Liver Stem Cells Acquire a Cardiomyocyte Structural and Functional Phenotype ex vivo ,
Am J Pathol
 165 , 135 , 2004
Rat
Rat, SD, 2 day 		Cardiomyocytes DMEM
Abdul-Ghani, M. , Suen, C. , Jiang, B. , Deng, Y. , Weldrick, J. , Putinski, C. , Brunette, S. , Fernando, P. , Lee, T. , Flynn, P. , Leenen, F. , Burgon, P. Stewart, D. and Megeney, L.
Cardiotrophin 1 Stimulates Beneficial Myogenic and Vascular Remodeling of the Heart. ,
Cell Res
 27 , 1195-1215 , 2017
Rat
Rat
(also bovine) 		Heart
Adrenal chromaffin
Paraneurons 		25mM HEPES buffered Locke's solution, CMF
Trifaro, J. , Tang, R. and Novas, M.
Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons ,
In Vitro Cell Dev Biol
 26 , 335 , 1990
Rat
Rat, Wistar, 1 day 		Cardiomyocytes M199
Shimizu Tatsuya, Yamato Masayuki, Isoi Yuki, Akutsu Takumitsu, Setomaru Takeshi, Abe Kazuhiko, Kikuchi Akihiko, Umezu Mitsuo, Okano Teruo.
Fabrication of pulsatile cardiac tissue grafts using a novel 3-dimensional cell sheet manipulation technique and temperature-responsive cell culture surfaces ,
Circ Res
 90 , e40 , 2002
Rat
Rat, Wistar, 1-4 day old 		Myocytes Gey's BSS
Mark, G. and Strasser, F.
Pacemaker Activity and Mitosis in Cultures of Newborn Rat Heart Ventricle Cells ,
Exp Cell Res
 44 , 217 , 1966
Rat
Rat, Wistar, male 		Cardiomyocytes L-15
Brinckmann, M. , Kaschina, E. , Altarche-Xifro, W. , Curato, C. , Timm, M. , Grzesiak, A. , Dong, J. , Kappert, K. , Kintscher, U. , Unger, T. and Li, J.
Estrogen Receptor Alpha Supports Cardiomyocytes Indirectly Through Post-Infarct Cardiac c-kit+ Cells. ,
J Mol Cell Cardiol
 47 , 66 , 2009
Rat
Rat, 1-3 day 		Neonatal rat cardiomyocytes DMEM/F12
Adderley SR. and Fitzgerald DJ.
Oxidative damage of cardiomyocytes is limited by extracellular regulated kinases 1/2-mediated induction of cyclooxygenase-2 ,
J Biol Chem
 274 , 5038 , 1999
Rat
SD, SHR or WKY 		Cardiomyocytes HBSS
MacGregor, R. , Klein, R. and Bansal, D.
Secretion of Plasminogen Activator Activity from Neonatal Rat Heart Cells is Regulated by Hormones and Growth Factors ,
Ann N Y Acad Sci
 752 , 331 , 1995
Rat
Rat,1-2 day 		Cardiomyocytes DMEM
Natarajan AR, Rong Q, Katchman AN. and Ebert SN.
Intrinsic cardiac catecholamines help maintain beating activity in neonatal rat cardiomyocyte cultures ,
Pediatr Res
 56 , 411 , 2004
Rat
Rat, adult, 300-370 g 		Cardiomyocytes KH buffer
Chouchani, E. , Pell, V. , Gaude, E. , Aksentijevic, D. , Sundier, S. , Robb, E. , Logan, A. , Nadtochiy, S. , Ord, E. , Smith, A. , Eyassu, F. , Shirley, R. , Hu, C. , Dare, A. , James, A. and Rogatti, S.
Ischaemic Accumulation of Succinate Controls Reperfusion Injury Through Mitochondrial ROS. ,
Nature
 515 , 431-435 , 2014
Rat
Rat, adult, female 		Myocytes Joklik's MEM
Nag, A.C. , Lee, M. and Kosiur, J.R.
Adult Cardiac Muscle Cells in Long-Term Serum-Free Culture:Myofibrillar Organization and Expression of Myosin Heavy Chain Isoforms ,
In Vitro Cell Dev Biol
 26 , 464 , 1990
Rat
Rat, Wistar, 1 day 		Cardiomyoctes HBSS
Kinugawa, K. , Kohmoto, O. , Yao, A. , Serizawa, T. and Takahashki, T.
Cardiac inducible nitric oxide synthase negatively modulates myocardial function in cultured rat myocytes ,
Am J Physiol
 272 , H35-47 , 1997
Rat
Rat, female, albino, 200-250 g 		Myocytes Kreb's Ringers phosphate
Nag, A. , Fischman, D. , Aumont, M. and Zak, R.
Studies of Isolated Adult Rat Heart Cells: The Surface Morphology and the Influence of Extracellular Calcium Ion Concentration on Cellular Viability ,
Tissue Cell
 9 (3) , 419 , 1977
Rat
Rat, neonatal 		Cardiomyocytes L-15
Smith, M. , Huang, Y. and Deshmukh, M.
Skeletal Muscle Differentiation Evokes Endogenous XIAP to Restrict the Apoptotic Pathway. ,
PLoS ONE
 4 , e5097 , 2009
Rat
Rat, 50 g 		Ventricular myocytes Medium 199
Aberle II NS. and Ren J.
Experimental Assessment of the Role of Acetaldehyde in Alcoholic Cardiomyopathy ,
Biol Proced Online
 5 , 1 , 2003
Rat
Rat, 1-2 day 		Cardiomyocytes MEM
Xie, L. , Pi, X. , Townley-Tilson, WH. , Li, N. , Wehrens, X. , Entman, M. , Taffet, G. , Mishra, A. , Peng, J. , Schisler, J. , Meissner, G. and Patterson, C.
PHD2/3-dependent Hydroxylation Tunes Cardiac Response to β-adrenergic Stress via Phospholamban. ,
J Clin Invest
 125 , 2759-71 , 2015
Rat
Rat, 1-2 day 		Cardiomyocytes DMEM/F-12
Pedram, A. , Razandi, M. , Aitkenhead, M. and Levin, E
Estrogen Inhibits Cardiomyocyte Hypertrophy in vitro. Antagonism of Calcineurin-related Hypertrophy through Induction of MCIP1 ,
J Biol Chem
 280 , 26339 , 2005
Rat
Rat, adult 		Sinoatrial nodal Tyrode solution
Zhang, Y. , Wang, Y. , Yanni, J. , Qureshi, M. , Logantha, S. , Kassab, S. , Boyett, M. , Gardiner, N. , Sun, H. , Howarth, F. and Dobrzynski, H.
Electrical Conduction System Remodeling in Streptozotocin-Induced Diabetes Mellitus Rat Heart. ,
Front Physiol
 10 , 826 , 2019
Rat
Rat, SD, female, 60-90 days 		Myocardial Joklik's MEM, Kreb's-Henseleit buffer, CF
Welder, A.A. , Grant, R. , Bradlaw, J. and Acosta, D.
A Primary Culture System of Adult Rat Heart Cells for the Study of Toxicologic Agents ,
In Vitro Cell Dev Biol
 27 , 921 , 1991
Rat
Rat, SD, 1 day 		Cardiomyocytes DMEM/F-12
Wagner DR, Combes A, McTiernan C, Sanders VJ, Lemster B, Feldman AM.
Adenosine inhibits lipopolysaccharide-induced cardiac expression of tumor necrosis factor-alpha ,
Circ Res
 82 , 47-56 , 1998
Rat
Rat, SD, male 		Aortic smooth muscle DMEM
Cornwell, T. and Lincoln, T.
Regulation of Intracellular Ca2+ Levels in Cultured Vascular Smooth Muscle Cells ,
J Biol Chem
 264 (2) , 1146 , 1989
Rat
Rat, SD, 1-2 day 		Cardiomyocytes L-15
Kim, M. , Oh, J. , Sakata, S. , Liang, I. , Park, W. , Hajjar, R. and Lebeche, D.
Role of Resistin in Cardiac Contractility and Hypertrophy. ,
J Mol Cell Cardiol
 45 , 270 , 2008
Rat
Rat, 1-2 day 		Cardiomyocytes M199 medium
Bueno OF, De Windt LJ, Tymitz KM, Witt SA, Kimball TR, Klevitsky R, Hewett TE, Jones SP, Lefer DJ, Peng CF, Kitsis RN. and Molkentin JD.
The MEK1-ERK1/2 signaling pathway promotes compensated cardiac hypertrophy in transgenic mice ,
EMBO J
 19(23) , 6341 , 2000
Rat
Rat, Wistar, 250-350g 		Ventricular myocytes Tyrode solution
Calderon-Sachez, E. , Domi­nguez-Rodriguez, A. , Lopez-Haldon, J. , Jimenez-Navarro, M. , Gomez, A. , Smani, T. and Ordonez, A.
Cardioprotective Effect of Ranolazine in the Process of Ischemia-reperfusion in Adult Rat Cardiomyocytes. ,
Rev Esp Cardiol (Engl Ed)
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Rat
Rat, 1 day 		Cardiomyocytes L-15
Yang Y, Liao H, Ke Q, Cai J, Xiao YF. and Morgan JP
Enhancement of nitric oxide production by methylecgonidine in cultured neonatal rat cardiomyocytes ,
Br J Pharmacol
 135 , 188 , 2002
Rat
Rat, 250 g 		Fibroblasts DMEM
Stoppel, W. , Gao, A. , Greaney, A. , Partlow, B. , Bretherton, R. , Kaplan, D. and Black, L
Elastic, Silk-Cardiac Extracellular Matrix Hydrogels Exhibit Time-Dependent Stiffening that Modulates Cardiac Fibroblast Response. ,
J Biomed Mater Res A
 104 , 3058 , 2016
Rat
Rat, 2-5 day 		Myocytes Saline A
Speicher, D. and McCarl, R.
Pancreatic Enzyme Requirements for the Dissociation of Rat Hearts for Culture ,
In Vitro
 10 , 30 , 1974
Rat
Rat, SD, male 		Ventricular myocytes DMEM
Stagg Mark A, Coppen Steven R, Suzuki Ken, Varela-Carver Anabel, Lee Joon, Brand Nigel J, Fukushima Satsuki, Yacoub Magdi H, Terracciano Cesare M.
Evaluation of frequency, type, and function of gap junctions between skeletal myoblasts overexpressing connexin43 and cardiomyocytes: relevance to cell transplantation ,
FASEB J
 20 , 744-6 , 2006
Rat
Rat, male, 6-12 months old 		Cardiac myocytes
  • Protease : 0.55 u/ml
Tyrode's solution
Kitta, K. , Day, R. , Ikeda, T. and Suzuki, Y.
Hepatocyte Growth Factor Protects Cardiac Myocytes Against Oxidative Stress-Induced Apoptosis ,
Free Radic Biol Med
 31 (7) , 902 , 2001
Rat
Rat, Wistar, neonatal 		Cardiomyocytes L-15
Butler, T. , Au, C. , Yang, B. , Egan, J. , Tan, Y. , Hardeman, E. , North, K. , Verkman, A. and Winlaw, D.
Cardiac Aquaporin Expression in Humans, Rats, and Mice. ,
Am J Physiol Heart Circ Physiol
 291 , H705 , 2006
Rat
Rat, SD, 2-4 day 		Cardiomyocytes, fibroblasts Medium 199
Entcheva, E. , Bien, H, Yin L. , Chung, C. , Farrell, M. and Kostov, Y.
Functional Cardiac Cell Constructs on Cellulose-based Scaffolding ,
Biomaterials
 25(26) , 5753 , 2004
Rat
Rat, SD, 8-10 week 		Ventricular myocytes Tyrode's Solution
Lee, J. , Ha, J. and Leem, C.
A Novel Nicotinamide Adenine Dinucleotide Correction Method for Mitochondrial Ca(2+) Measurement with FURA-2-FF in Single Permeabilized Ventricular Myocytes of Rat. ,
Korean J Physiol Pharmacol
 19 , 373-82 , 2015
Rat
Rat, 1 day 		Cardiomyocytes F-10
Chen, Z. , Ge, Y. and Kang J.
Down-regulation of the M6P/IGF-II Receptor Increases Cell Proliferation and Reduces Apoptosis in Neonatal Rat Cardiac Myocytes ,
BMC Cell Biol
 5 , 15 , 2004
Rat
Rat, SD, adult, male, 200-250 g 		Myocytes Joklik MEM, CF
Frangakis, C. , Bahl, J. , McDaniel, H. and Bressler, R.
Tolerance to Physiological Calcium by Isolated Mycocytes from the Adult Rat Heart; An Improved Cellular Preparation ,
Life Sci
 27 , 815 , 1980
Rat
Rat, SD, 200-300 g 		Cardiomyocytes Joklik's MEM
Swift Luther, McHowat Jane, Sarvazyan Narine.
Inhibition of membrane-associated calcium-independent phospholipase A2 as a potential culprit of anthracycline cardiotoxicity ,
Cancer Res
 63 , 5992-8 , 2003
Rat
Rat, Lewis, adult male, 300-350-g 		Aorta, smooth muscle Medium 199 with 20% FBS medium
Kim, B. , Nikolovski, J. , Bonadio, J. , Smiley, E. and Mooney, D.
Engineered Smooth Muscle Tissues: Regulating Cell Phenotype with the Scaffold ,
Exp Cell Res
 251 , 318 , 1999
Rat
Rat, SD, 2 day 		Cardiomyocytes DMEM/F-12
Jang, J. , Ku, S. , Kim, J. , Choi, K. , Kim, Y. , Kim, H. , Oh, S. , Lee, E. , Cho, H. , Song, Y. , Lee, S. , Lee, S. , Suh, C. , Kim, S. , Moon, S. and Choi, Y.
Notch Inhibition Promotes Human Embryonic Stem Cell-Derived Cardiac Mesoderm Differentiation. ,
Stem Cells
 26 , 2782 , 2008
Rat
Rat, neonatal, adult, 17 day 		Ventricular cardiomyocytes DMEM
Lam ML, Bartoli M. and Claycomb WC.
The 21-day postnatal rat ventricular cardiac muscle cell in culture as an experimental model to study adult cardiomyocyte gene expression ,
Mol Cell Biochem
 229 , 51 , 2002
Rat
Rat, SD, 1 day 		Cardiomyocytes HBSS
Clark, A. and Naya, F.
MicroRNAs in the Myocyte Enhancer Factor 2 (MEF2)-regulated Gtl2-Dio3 Noncoding RNA Locus Promote Cardiomyocyte Proliferation by Targeting the Transcriptional Coactivator Cited2. ,
J Biol Chem
 290 , 23162-72 , 2015
Rat
Rat, wistar, 2 day 		Cardiomyocytes DMEM-F12
Cowan DB, Poutias DN, Del Nido PJ. and McGowan FX Jr
CD14-independent activation of cardiomyocyte signal transduction by bacterial endotoxin ,
Am J Physiol Heart Circ Physiol
 279 , H619 , 2000
Rat
Rat, SD, male, 250 - 350 g 		Myocytes, ventricular Joklik's solution with and without calcium
De Young, M. , Giannattasio, B. and Scarpa, A.
Isolation of Calcium-Tolerant Atrial And Ventricular Myocytes From Adult Rat Heart , Methods Enzymology Vol. 173 , 662 , 1989
Rat
Rat, SD, 175-200g 		Cardiomyocytes Krebs-Ringer
Tardif Annie, Julien Nathalie, Chiasson Jean-Louis, Coderre Lise.
Stimulation of glucose uptake by chronic vanadate pretreatment in cardiomyocytes requires PI 3-kinase and p38 MAPK activation ,
Am J Physiol/Endo
 284 , E1055-64 , 2003
Rat
Rat 		Thoracic aorta, smooth muscle (see reference)
Zilberman, A. , Dave, V. , Miano, J. , Olson, E. and Periasamy, M.
Evolutionary Conserved Promoter Region Containing CArg-Like Elements is Crucial for Smooth Muscle Myosin Heavy Chain Gene Expression ,
Circ Res
 82 , 566 , 1998
Rat
Rat, SD, male, 225-250g 		Cardiomyocytes J-MEM
Kubli, D. , Quinsay, M. , Huang, C. , Lee, Y. and Gustafsson, A.
Bnip3 Functions as a Mitochondrial Sensor of Oxidative Stress During Myocardial Ischemia and Reperfusion. ,
Am J Physiol Heart Circ Physiol
 295 , H2025 , 2008
Rat
Rat, Winstar, 1-2 day 		Ventricular myocytes DMEM
Maki T, Horio T, Yoshihara F, Suga S, Takeo S, Matsuo H. and Kangawa K
Effect of neutral endopeptidase inhibitor on endogenous atrial natriuretic peptide as a paracrine factor in cultured cardiac fibroblasts ,
Br J Pharmacol
 131(6) , 1204 , 2000
Rat
Rat, SD, 2-3 day 		Cardiomyocytes DMEM
Fang, R, Qiao, S. , Liu, Y. , Meng, Q. , Chen, X. , Song, B. , Hou, X. and Tian, W.
Sustained Co-Delivery of BIO and IGF-1 by a Novel Hybrid Hydrogel System to Stimulate Endogenous Cardiac Repair in Myocardial Infarcted Rat Hearts. ,
Int J Nanomedicine
 10 , 4691-703 , 2015
Rat
Rat, fetal or 3-day 		Cardiomyocytes HEPES
Tamamori-Adachi Mimi, Ito Hiroshi, Sumrejkanchanakij Piyamas, Adachi Susumu, Hiroe Michiaki, Shimizu Masato, Kawauchi Junya, Sunamori Makoto, Marumo Fumiaki, Kitajima Shigetaka, Ikeda Masa-Aki.
Critical role of cyclin D1 nuclear import in cardiomyocyte proliferation ,
Circ Res
 92 , e12-9 , 2003
Rat
Rat, neonatal 		Cardiomyocytes L-15
Webster DR. and Patrick DL.
Beating rate of isolated neonatal cardiomyocytes is regulated by the stable microtubule subset ,
Am J Physiol Heart Circ Physiol
 278 , H1653 , 2000
Rat
Rat, adult, male, 250 g 		Myocytes Saline A
Vahouny, G.V. , Wei, R. , Starkweather, R. and Davis, C.
Preparation of Beating Heart Cells From Adult Rats ,
Science
 167 , 1616 , 1970
Rat
Rat, SD, 300-350 g 		Cardiomyocytes see reference
Yu L, Netticadan T, Xu YJ, Panagia V, Dhalla NS.
Mechanisms of lysophosphatidylcholine-induced increase in intracellular calcium in rat cardiomyocytes ,
J Pharmacol Exp Ther
 286 , 1-8 , 1998
Rat
Rat, 10-15 newborn, 1-2 days old 		Atrial HBSS or EBSS
Nemec, J. , Wickman, K. and Clapham, D.
GBy Binding Increases the Open Time of IKACh: Kinetic Evidence for Multiple GBy Binding Sites ,
Biophys J
 76 , 246 , 1999
Rat
Rat, Wistar, 1-3 day 		Cardiomyocytes L-15
Castillo, A. , Ruzmetov, N. , Harvey, K. , Stillwell, W. , Zaloga, G. and Siddiqui, R.
Docosahexaenoic Acid Inhibits Protein Kinase C Translocation/Activation and Cardiac Hypertrophy in Rat Cardiomyocytes. ,
J Mol Genet Med
 1 , 18 , 2005
Rat
Rat, SD, adult female 		Heart microvascular cells L-15
Puri S, Bansal DD, Uskokovic MR. and MacGregor RR.
Induction of tissue plasminogen activator secretion from rat heart microvascular cells by fM 1,25(OH)(2)D(3) ,
Am J Physiol/Endo
 278(2) , E293 , 2000
Rat
Rat, SD 		Valve interstitial cells DMEM
Horne, T. , VandeKopple, M. , Sauls, K. , Koenig, S. , Anstine, L. , Garg, V. , Norris, R. and Lincoln, J.
Dynamic Heterogeneity of the Heart Valve Interstitial Cell Population in Mitral Valve Health and Disease ,
J Cardiovasc Dev Dis
 2 , 214 , 2015
Rat
Rat, adult, 220-280g 		Cardiomyocytes Krebs-Henseleit, CF
Gordon Jennifer M, Dusting Gregory J, Woodman Owen L, Ritchie Rebecca H.
Cardioprotective action of CRF peptide urocortin against simulated ischemia in adult rat cardiomyocytes ,
Am J Physiol Heart Circ Physiol
 284 , H330-6 , 2003
Rat
Rat, SD, male 		Cardiomyocytes Krebs-Henseleit
Dai L, Brookes PS, Darley-Usmar VM. and Anderson PG.
Bioenergetics in cardiac hypertrophy: mitochondrial respiration as a pathological target of NO ,
Am J Physiol/Heart
 281 , H2261-H2269 , 2001
Rat
Rat, male/female, 200-250 g 		Muscle Krebs Ringer phosphate buffer, CMF
Nag, A. and Cheng, M.
Adult Mammalian Cardiac Muscle Cells in Culture ,
Tissue Cell
 13 , 515 , 1981
Rat
Rat, SD, 200-250 g 		Ventricular myocytes and mesenteric arterial SMC Krebs-Ringer
Lu Tong, Ye Dan, Wang Xiaoli, Seubert John M, Graves Joan P, Bradbury J Alyce, Zeldin Darryl C, Lee Hon-Chi.
Cardiac and vascular KATP channels in rats are activated by endogenous epoxyeicosatrienoic acids through different mechanisms ,
J Physiol
 575 , 627-44 , 2006
Rat
Rat, newborn 		Atrial DMEM
Kim, D. and Pleumsamran, A.
Cytoplasmic Unsaturated Free Fatty Acids Inhibit ATP-Dependent Gating of the G Protein-gated K+ Channel ,
J Gen Physiol
 115 , 287 , 2000
Rat
Rat, 3 day 		Cardiomyocytes PBS
Ye, K. , Sullivan, K. and Black, L.
Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering. ,
J Vis Exp
 55 , 3251 , 2011
Rat
Rat, SD, newborn 		Cardiac myocytes L-15
Rahman A, Alam M, Rao S, Cai L, Clark LT, Shafiq S. and Siddiqui MA
Differential effects of doxorubicin on atrial natriuretic peptide expression in vivo and in vitro ,
Biol Res
 34(3-4) , 195 , 2001
Rat
Rat, SD, 3-4 month 		Cardiomyocytes DMEM
Liu, L. , Wang, Y. , Cao, Z. , Wang, M. , Liu, X. , Gao, T. , Hu, Q. , Yuan, W. and Lin, L.
Up-regulated TLR4 in Cardiomyocytes Exacerbates Heart Failure after Long-Term Myocardial Infarction. ,
J Cell Mol Med
 19 , 2728 , 2015
Rat
Rat, SD, albino, male, 180-230 g 		Heart ventricles, beating Phosphate buffer
Glick, M. , Burns, A. and Reddy, W.
Dispersion and Isolation of Beating Cells from Adult Rat Heart ,
Anal Biochem
 61 , 32 , 1974
Rat
Rat, 1-2 day 		Cardiomyocytes RPMI
Matsui, T. , Li, Ling, Del Monte, F. , Fukui, Y. , Franke, T. , Hajjar, R. and Rosenzweig, A.
Adenoviral gene transfer of activated phosphatidylinositol 3'-kinase and Akt inhibits apoptosis of hypoxic cardiomyocytes in vitro ,
Circulation
 100 , 2373-9 , 1999
Rat
Rat, SD, 2-4 day old 		Cardiomyocytes
Fibroblasts 		HBSS
Toraason, M. , Luken, M. , Breitenstein, M. , Krueger, J. and Biagini, R.
Comparative Toxicity of Allylamine and Acrolein in Cultured Myocytes and Fibroblasts from Neonatal Rat Heart ,
Toxicology
 56 , 107 , 1989
Rat
Rat, SD, fetal and neonatal 		Cardiomyocytes PBS
Li RK, Mickle DA, Weisel RD, Zhang J, Mohabeer MK.
In vivo survival and function of transplanted rat cardiomyocytes ,
Circ Res
 78 , 283-8 , 1996
Rat
Rat, Harlan SD, pup 		Cardiomyocytes DMEM/Ham's F-12
Schwartzbauer, G. and Robbins, J.
The Tumor Suppressor Gene PTEN Can Regulate Cardiac Hypertrophy and Survival ,
J Biol Chem
 276 , 35786 , 2001
Rat
Rat, SD, male 		Cardiomyocytes Krebs
Mellor, K. , Bell, J. , Wendt, I. , Davidoff, A. , Ritchie, R. and Delbridge, L.
Fructose Modulates Cardiomyocyte Excitation-Contraction Coupling and Ca Handling In Vitro. ,
PLoS ONE
 6 , e25204 , 2011
Rat
Rat, 1-2 day 		Cardiomyocytes DMEM
Reinecke H, Zhang M, Bartosek T. and Murry CE.
Survival, integration, and differentiation of cardiomyocyte grafts: a study in normal and injured rat hearts ,
Circulation
 100(2) , 193 , 1999
Rat
Rat, neonatal 		Cardiomyocytes HBSS
Song, D. , Lee, K. , Ryu, J. , Jeon, H. and Kim, D.
The Molecular Interaction of Heart LIM Protein (HLP) with RyR2 and Caveolin-3 is Essential for Ca(2+)-Induced Ca(2+) Release in the Heart. ,
Biochem Biophys Res Commun
 463 , 975-81 , 2015
Rat
Rat,SD, albino, 10-14 weeks 		Myocytes Krebs Ringer bicarbonate buffer
Berg, I. , Guse, A.H. and Gercken, G.
Carbamoylcholine-Induced Accumulation of Inositol Mono-,Bis-,Tris-, And Tetrakisphosphates In Isolated Cardiac Myocytes From Adult Rats ,
Biochim Biophys Acta
 1010 , 100 , 1989
Rat
Rat, neonatal 		Cardiac myocytes HBSS
Chen Hsiao-Huei, Mullett Steven J, Stewart Alexandre F R.
Vgl-4, a novel member of the vestigial-like family of transcription cofactors, regulates alpha1-adrenergic activation of gene expression in cardiac myocytes ,
J Biol Chem
 279 , 30800-6 , 2004
Rat
Rat, adult, 150-200 g 		Myocytes, atria Kreb's Ringer bicarbonate - HEPES buffer
McMahon, K.K.
A Study of Adult Rat Atrial Myocyte Attachment to Extricellular Matrix Components and Long Term Culture , Biology of Isolated Adult Cardiac Myocytes , (Clark, Decker, Borg, Eds) , Elsevier Science Publishing Co. Inc. , 318 , 1988
Rat
Rat, SD, 1-2 day 		Cardiomyocytes HBSS
Arutunyan A, Webster D, Swift L, Sarvazyan N.
Localized injury in cardiomyocyte network: a new experimental model of ischemia-reperfusion arrhythmias ,
Am J Physiol Heart Circ Physiol
 280 , H1905-15 , 2001
Rat
Rat, adult 		Myocytes Perfusing solution
Moustafa, E. , Skomedal, T. , Osnes, J. and Oye, I.
Cyclic Amp Formation and Morphology of Myocardial Cells Isolated from Adult Heart: Effect of Ca2+ and Mg2+ ,
Biochim Biophys Acta
 421 , 411 , 1976
Rat
Rat, adult 		Myocytes Media 199
Xu, X. and Colecraft, H.
Primary Culture of Adult Rat Heart Myocytes. ,
J Vis Exp
 28 , 1308 , 2009
Rat
Rat, adult 		Ventricular myocytes Tyrode solution
Ren J. and Wold LE.
Measurement of Cardiac Mechanical Function in Isolated Ventricular Myocytes from Rats and Mice by Computerized Video-Based Imaging ,
Biol Proced Online
 3 , 43 , 2001
Rat
Rat, neonatal 		Cardiomyocytes HBSS
Zhang, B. , Montgomery, M. , Davenport-Huyer, L. , Korolj, A. and Radisic, M.
Platform Technology for Scalable Assembly of Instantaneously Functional Mosaic Tissues. ,
Sci. Adv.
 1 , e1500423 , 2015
Rat
Rat, Wistar, 2-4 day neonates 		Myoblast, cardiac Standardized Medium
(see reference)
Grynberg, A. , Degois, M. , Guenot, L. and Athias, P.
Primary Rat Cardiac Cell Culture: Diet of the Mother Rats as a Determinant Parameter of Cardiomyoblast Production from Neonates ,
Biol Cell
 57 , 89 , 1986
Rat
Rat, neonatal 		Cardiomyocytes DMEM/M199
Guo K, Searfoss G, Krolikowski D, Pagnoni M, Franks C, Clark K, Yu KT, Jaye M, Ivashchenko Y.
Hypoxia induces the expression of the pro-apoptotic gene BNIP3 ,
Cell Death Differ
 8 , 367-76 , 2001
Rat
Rat, SD 		Myocytes Kreb's Ringer w/ calcium
Buxton, I.L.O. and Doggwiler, K.O.
Alpha1-Adrenergic Receptor Signal Transduction in the Adult Rat Cardiac Myocyte , Biology of Isolated Adult Cardiac Myocytes , (Clark, Decker, Borg, eds) , 298 , 1988
Rat
Rat, adult, male, 150-200g 		Myocytes MEM CF
Grosso, D. , Frangakis, C. , Carlson, E. and Bressler, R.
Isolation and Characterization of Myocytes From the Adult Rat Heart ,
Prep Biochem
 7 (5) , 383 , 1977
Rat
Rat, neonatal, 5-day 		Cardiomyocytes DMEM
Vanwinkle, W. , Snuggs, M. and Buja, L.
Cardiogel: A Biosynthetic Extracellular Matrix for Cardiomyocyte Culture ,
In Vitro Cell Dev Biol Anim
 32 , 478 , 1996
Rat
Rat, SD, 1-2 day 		Cardiomyocytes DMEM
Zhang, B. , Green, J. , Murthy, S. and Radisic, M.
Label-Free Enrichment of Functional Cardiomyocytes using Microfluidic Deterministic Lateral Flow Displacement. ,
PLoS ONE
 7 , e37619 , 2012
Rat
Rat, adult and neonatal 		Cardioomyocytes DMEM/medium 199
Richards SM, Jaconi ME, Vassort G. and Puceat M.
A spliced variant of AE1 gene encodes a truncated form of Band 3 in heart: the predominant anion exchanger in ventricular myocytes ,
J Cell Sci
 112 , 1519 , 1999
Rat
Rat, Wistar, 200g 		Ventricular myocytes HEPES
Miller, L. , Greensmith, D. , Sankaranarayanan, R. , O'Neill, S. and Eisner D.
The Effect of 2,5-di-(tert-butyl)-1,4-benzohydroquinone (TBQ) on Intracellular Ca2+ Handling in Rat Ventricular Myocytes. ,
Cell Calcium
 58 , 208-14 , 2015
Rat
Rat, albino, male, 250 g 		Myocytes Hank's solution, CF
Berry, M. , Friend, D. and Scheuer, J.
Morphology and Metabolism of Intact Muscle Cells Isolated from Adult Rat Heart ,
Circ Res
 26 , 679 , 1970
Rat
Rat, male, 250-300 g 		Cardiomyocytes HBSS
Hunton DaciaL, Zou LuYun, Pang Yi, Marchase Richard B.
Adult rat cardiomyocytes exhibit capacitative calcium entry ,
Am J Physiol Heart Circ Physiol
 286 , H1124-32 , 2004
Rat

Rat, 1-2 day

 Cardiomyocytes DMEM
Yang, M. , Abudureyimu, M. , Wang, X. , Zhou, Y. , Zhang, Y. and Ren, J.
PHB2 ameliorates Doxorubicin-induced cardiomyopathy through interaction with NDUFV2 and restoration of mitochondrial complex I function. , Redox Biol Vol. 65 , 102812 , 2023
Rat
Rat, SD, female, 12 weeks 		Myocytes Krebs Ringer bicarbonate buffer
Piper, H.M. , Probst, I. , Schwartz, P. , Hutter, F.J. and Spieckermann, P.G.
Culturing of Calcium Stable Adult Cardiac Myocytes ,
J Mol Cell Cardiol
 14 , 397 , 1982
Rat
Rat, SD, male, 250-300 g 		Cardiomyocytes Cardioplegic solution
Head, B. , Patel, H. , Roth, D. , Lai, N. , Niesman, I. , Farquhar, M. and Insel, P.
G-Protein-Coupled Receptor Signaling Components Localize in Both Sarcolemmal and Intracellular Caveolin-3-Associated Microdomains in Adult Cardiac Myocytes. ,
J Biol Chem
 280 , 31036-44 , 2005
Rat
Rat, SD, adult, female, 180-220 g 		Cardiomyocytes (see reference)
Fischer, Y. , Rose, H. and Kammermeier. H.
Highly Insulin-Responsive Isolated Rat Heart Muscle Cells Yielded By a Modified Isolation Method ,
Life Sci
 49 , 1679 , 1991
Rat
Rat, neonatal 		Cardiomyocytes L-15
Dittami, G. , Rajguru, S. , Lasher, R. , Hitchcock, R. and Rabbitt, R.
Intracellular Calcium Transients Evoked by Pulsed Infrared Radiation in Neonatal Cardiomyocytes. ,
J Physiol
 589 , 1295-306 , 2011
Rat
Rat, Wistar, male 		Cardiomyocytes Joklik MEM
Sambandam N, Abrahani MA, St Pierre E, Al-Atar O, Cam MC. and Rodrigues B.
Localization of lipoprotein lipase in the diabetic heart: regulation by acute changes in insulin ,
Arterioscler Thromb Vasc Biol
 19(6) , 1526 , 1999
Rat
Rat, adult 		Cardiomyocytes Tyrode solution
Savi, M. , Bocchi, L. , Mena, P. , Dall'Asta, M. , Crozier, A. , Brighenti, F. , Stilli, D. and Del Rio, D.
In vivo Administration of Urolithin A and B Prevents the Occurrence of Cardiac Dysfunction in Streptozotocin-induced Diabetic Rats. ,
Cardiovasc Diabetol
 16 , 80 , 2017
Rat
Rat, Hebrew Un strain, 3 & 17 months, 150-210 g 		Myocytes Versene buffer
Bierman, E.L. , Stein, O. and Stein, Y.
Lipoprotein Uptake and Metabolism by Rat Aortic Smooth Muscle Cells in Tissue Culture ,
Circ Res
 35 , 136 , 1974
Rat
Rat, SD, 300-350 g 		Cardiomyocytes DMEM
Niederbichler Andreas D, Hoesel Laszlo M, Westfall Margaret V, Gao Hongwei, Ipaktchi Kyros R, Sun Lei, Zetoune Firas S, Su Grace L, Arbabi Saman, Sarma J Vidya, Wang StewartC, Hemmila MarkR, Ward PeterA.
An essential role for complement C5a in the pathogenesis of septic cardiac dysfunction ,
J Exp Med
 203 , 53-61 , 2006
Rat
Rat, SD, female, 12 wks, 250 g 		Myocytes
Ventricular myocardium 		Kreb's Ringer bicarbonate buffer
Powell, T. , Terrar, D.A. and Twist, V.W.
Electrical Properties of Individual Cells Isolated From Adult Rat Ventricular Myocardium ,
J Physiol
 302 , 131 , 1980
Rat
Rat, neonatal 		Cardiomyocytes DMEM
Tamamori-Adachi, M. , Hayashida, K. , Nobori, K. , Omizu, C. , Yamada, K. , Sakamoto, N. , Kamura, T. , Fukuda, K. , Ogawa, S. , Nakayama, K. and Kitajima, S.
Down-Regulation of p27 Promotes Cell Proliferation of Rat Neonatal Cardiomyocytes Induced by Nuclear Expression of Cyclin D1 and CDK4 ,
J Biol Chem
 2279 , 50429 , 2004
Rat
Rat, SD, male, 150-200 g 		Myocytes (see reference)
Pretlow II, T. , Glick, M. and Reddy, W.
Separation of Beating Cardiac Myocytes from Suspensions of Heart Cells ,
Am J Pathol
 67 (2) , 215 , 1972
Rat
Rat, neonatal 		Cardiomyocytes DMEM/M199
Guan, X. , Delo, D. , Atala, A. and Soker, S.
In Vitro Cardiomyogenic Potential of Human Amniotic Fluid Stem Cells. ,
J Tissue Eng Regen Med
 5 , 220 , 2011
Rat
Rat, adult 		Cardiomyocytes see reference
Louch, W. , Sheehan, K. and Wolska, B.
Methods in Cardiomyocyte Isolation, Culture, and Gene Transfer. ,
J Mol Cell Cardiol
 51 , 288-98 , 2011
Rat
Rat, SD, 250-300 g 		Cardiomyocytes Joklik's
Sharma VK, Colecraft HM, Wang DX, Levey AI, Grigorenko EV, Yeh HH. and Sheu SS
Molecular and functional identification of m1 muscarinic acetylcholine receptors in rat ventricular myocytes ,
Circ Res
 79(1) , 86 , 1996
Rat
Rat, SD, 1-2 day 		Cardiomyocytes DMEM/F-12
Rutering, J. , Ilmer, M. , Recio, A. , Coleman, M. , Vykoukal, J. and Alt, E.
Improved Method for Isolation of Neonatal Rat Cardiomyocytes with Increased Yield of C-Kit+ Cardiac Progenitor Cells. ,
J Stem Cell Res Ther
 5 , 1-8 , 2015
Rat
Rat, SD, adult, female, 175 - 225 g 		Myocytes Joklik's medium
Bugaisky, L.B. and Zak, R.
Differentiation of Adult Rat Cardiac Myocytes in Cell Culture ,
Circ Res
 64 , 493 , 1989
Rat
Rat, female, 250-300 g 		Ventricular myocytes Ringer solution
Liu Qinghang, Hofmann Polly A.
Antiadrenergic effects of adenosine A(1) receptor-mediated protein phosphatase 2a activation in the heart ,
Am J Physiol Heart Circ Physiol
 283 , H1314-21 , 2002
Rat
Rat, SD, male, 250-350 g 		Myocytes, heart Bicarbonate-buffered medium
Kim, D. and Smith, T.W.
Temporal Variation in Contractile State And [Ca++] in Isolated Adult Rat And Guinea Pig Cardiac Myocytes , Biology of Isolated Adult Cardiac Myocytes , (Clark, Decker, Borg, eds) , Elsevier Science Publishing Co. Inc. , 370 , 1988
Rat
Rat, 2-3 day 		Cardiomyocytes DMEM
LaFramboise, W. , Scalise, D. , Stoodley, P. , Graner, S. , Guthrie, R. , Magovern, J. and Becich, M.
Cardiac Fibroblasts Influence Cardiomyocyte Phenotype in Vitro. ,
Am J Physiol Cell Physiol
 292 , C1799-808 , 2007
Rat
Rat, 10 wk 		Myocytes Saline
Bishop, S. , Oparil, S. , Reynolds, R. and Drummond, J.
Regional Myocyte Size in Normotensive and Spontaneously Hypertensive Rats ,
Hypertension
 1 (4) , 378 , 1979
Rat
Rat, SD, timid-pregnant 		Myocytes DMEM
Freerksen, D. , Schroedl, N. and Hartzell, C.
Control of Enzyme Activity Levels by Serum and Hydrocortisone in Neonatal Rat Heart Cells Cultured in Serum-Free Medium ,
J Cell Physiol
 120 , 126 , 1984
Rat
Rat 		Cardiomyocytes MEM
Hennessey, J. , Wei, E. and Pitt, G.
Fibroblast Growth Factor Homologous Factors Modulate Cardiac Calcium Channels. ,
Circ Res
 113 , 381-8 , 2013
Rat
Rat, SD, male, 250-300g 		Cardiomyocytes M199
Sun L, Chang J, Kirchhoff SR. and Knowlton AA.
Activation of HSF and selective increase in heat-shock proteins by acute dexamethasone treatment ,
Am J Physiol Heart Circ Physiol
 278(4) , H1091 , 2000
Rat
Rat, neonatal 		Cardiomyocytes L-15
Sakurai, T. , Lanahan, A. , Woolls, M. , Li, N. , Tirziu, D. and Murakami, M.
Live Cell Imaging of Primary Rat Neonatal Cardiomyocytes Following Adenoviral and Lentiviral Transduction using Confocal Spinning Disk Microscopy. ,
J Vis Exp
 , e51666 , 2014
Rat
Rat, female, adult, 200 - 300 g 		Myocytes Joklik's MEM
Claycomb, W.C. and Palazzo, M.C.
Culture of The Terminally Differentiated Adult Cardiac Muscle Cell: A Light and Scanning Electron Microscope Study ,
Dev Biol
 80 , 466 , 1980
Rat
Rat, Wistar, female 		Ventricular myocytes Ringer solution
Pyle, W, Lester, J. and Hofmann, P.
Effects of Kappa-opioid receptor activation of myocardium ,
Am J Physiol Heart Circ Physiol
 281 , H669 , 2001
Rat
Rat, SD, male, 200-250 g 		Myocytes
Ventricles 		Joklik's MEM
Laughlin, M. , Schaeffer, M. and Sturek, M.
Effect of Exercise Training on Intracellular Free Ca2+ Transients in Ventricular Myocytes of Rats ,
Am J Physiol
 73 , 1141 , 1992
Rat
Rat, SD, 200-250 g 		Cardiomyocytes Krebs-Henseleit
Farkas, A. , Acsai, K. , Nagy, N. , Toth, A. , Fulop, F. , Seprenyi, G. , Birinyi, P. , Nanasi, P. , Forster, T. , Csanady, M. , Papp, J. , Varrro, A. and Farkas, A.
Na(+)/Ca(2+) Exchanger Inhibition Exerts a Positive Inotropic Effect in the Rat Heart, but Fails to Influence the Contractility of the Rabbit Heart. ,
Br J Pharmacol
 154 , 93 , 2008
Shellfish
Clams, 3.0 to 4.5 cm 		Myocytes
Fibroblasts 		L15 medium
Wen, C. , Kou, G. and Chen, S.
Cultivation of Cells From the Heart of the Hard Clam, Merretrix lusoria (Roding) ,
J Tiss Cul Meth
 15 , 123 , 1993
Shellfish
Pacific Oysters 		Haemocytes L15 medium
Le Duff, R. , Lipart, C. and Renault, T.
Primary Culture of Pacific Oyster, Crassostrea gigas, heart cells ,
J Tiss Cul Meth
 16 , 67 , 1994

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.