Lung Tissue Tables

Species Cells Enzyme(s) Medium Reference
Bovine
Bovine
Endothelial
Pulmonary
PBS, CMF
Del Vecchio, P. , Siflinger-Birnboim, A. , Belloni, P. , Holleran, L. , Lum, H. and Malik, A.
Guinea-Pig
Guinea-pig, male, 250-300 g
Alveolar type II PBS
(see reference)
Sikpi, M. , Nair, C. , Johns, A. and Das, S.
Guinea-Pig
Guinea-pig, male, 100 g
Single cells CF salt solution
Human
Human
Pulmonary endothelial RPMI
Mackay, L. , Dodd, S. , Dougall, I. , Tomlinson, W. , Lordan, J. , Fisher, A. and Corris, P.
Human
Human
Aveolar PBS
Bahmed, K. , Boukhenouna, S. , Karim, L. , Andrews, T. , Lin, J. , Powers, R. , Wilson, M. , Lin, C. , Messier, E. , Reisdorph, N. , Powell, R. , Tang, H. , Mason, R. , Criner, G. and Kosmider, B.
Human
Human fetuses, 80 day
(also swine fetuses, 70 day, adult Amer Dutch, 250 day)
Lung HBSS
Human
Human, 16-24 week therapeutic abortions
Alveolar type II
Fetal
Ham's F-12, Eagle's MEM
Liley, H. , Ertsey, R. , Gonzales, L. , Odom, M. , Hawgood, S. , Dobbs, L. and Ballard, P.
Human
Human
Epithelial Medium 199
Lechner, J. , Haugen, A. , McClendon, I. and Pettis, E.
Human
Human, 12-16 wk old embryo
Fibroblasts Eagle's MEM
Human
Human
Lung
Fibroblasts
DMEM
Zhu, Y. , Skold, C. , Liu, X. , Wang, H. , Kohyama. , Wen, F. , Ertl, R. and Rennard, S.
Human
Human
Lung DMEM
Fujino, N. , Kubo, H. , Suzuki, T. , Ota, C. , Hegab, A. , He, M. , Suzuki, S. , Suzuki, T. , Yamada, M. , Kondo, T. , Kato, H. and Yamaya, M.
Human
Human
Alveolar epithelial Bronchial Epithelial Growth Medium
Barkauskas, C. , Cronce, M. , Rackley, C. , Bowie, E. , Keene, D. , Stripp, B. , Randell, S. , Noble, P. and Hogan, B.
Human
Human
Lung endothelial PBS
Comhair, S. , Xu, W. , Mavrakis, L. , Aldred, M. , Asosingh, K. and Erzurum, S.
Human Primary Lung Endothelial Cells in Culture. ,
Am J Respir Cell Mol Biol
46
, 723-30 , 2012
Human
Human
Lung tumor DMEM/F12
Quatromoni, J. , Singhal, S. , Bhojnagarwala, P. , Hancock, W. , Albelda, S. and Eruslanov, E.
Mouse
Mouse
Lung HBSS
Zhao, J. , Zhao, J. , Van Rooijen, N. and Perlman, S.
Mouse
Mouse
Lung PBS
Yu, Y. , O'Koren, E. , Holten, D. , Kan, M. , Kojin, D. , Nelson, E. , Que, L. and Gunn, M.
Mouse
Mouse
Dendritic HBSS
Flano, E. , Jewell, N. , Durbin, R. and Durbin, J.
Methods Used to Study Respiratory Virus Infection. , Curr Protoc Cell Biol Vol. Chapter 26 , Unit 26.3 , 2009
Mouse
Mouse
Lung HBSS
Misharin, A. , Morales-Nebreda, L. , Mutlu, G. , Budinger, G. and Perlman, H.
Mouse
Mouse
Lung draining HBSS
Rayamajhi, M. , Redente, E. , Condon, T. , Gonzalez-Juarrero, M. , Riches, D. and Lenz, L.
Mouse
2-3 month
Lung endothelial DMEM
Wang, J. , Niu, N. , Xu, S. and Jin, Z.
Mouse
Mouse, 6-10 week
Murine pulmonary endothelial HBSS
Andonegui G. , Bonder C. , Green F. , Mullaly S. , Zbytnuik L. , Raharjo E. and Kubes P.
Mouse
Mouse
Lung PBS
Mouse
Mouse
Lung PBS
Braza, F. , Dirou, S. , Forest, V. , Sauzeau, V. , Hassoun, D. , Chesne, J. , Cheminant-Muller, M. , Sagan, C. , Magnan, A. and Lemarchand, P.
Mouse
Mouse
Pulmonary T lymphocytes RPMI medium
Huaux F, Liu T, McGarry B, Ullenbruch M. and Phan SH.
Mouse
Mouse, 8-10 week
Lung mesenchymal stem HBSS
Chow, K. , Jun, D. , Helm, K. , Wagner, D. and Majka, S.
Mouse
Mouse, 8-12 week
Epithelial, Endothelial PBS
Singer, B. , Mock, J. , D'Alessio, F. , Aggarwal, N. , Mandke, P. , Johnston, L. and Damarla, M.
Mouse
Mouse
Alveolar epithelial cells DMEM
Paine R 3rd, Wilcoxen SE, Morris SB, Sartori C, Baleeiro CE, Matthay MA. and Christensen PJ
Mouse
Mouse, 6-16 week
Mononuclear RPMI 1640
Abonia, J. , Hallgren, J. , Jones, T. , Shi, T. , Xu, Y. , Koni, P. , Flavell, R. , Boyce, J. , Austen, F. and Gurish, M.
Mouse
Mouse, 6 week
Alves CarlosRoberto, Souza RaquelSantos de, Charret KarenDos Santos, Côrtes LuziaMonteiro de Castro, Sá-Silva MatheusPereira de, Barral-Veloso Laura, Oliveira LuizFilipe Gonçalves, da Silva FranklinSouza.
Mouse
Mouse, 6-8 week
Lung and lymph node cells RPMI 1640
Vermaelen KY, Carro-Muino I, Lambrecht BN. and Pauwels RA.
Mouse
Mouse
Lung PBS
Guimond, D. , Cam, N. , Hirve, N. , Duan, W. , Lambris, J. , Croft, M. and Tsoukas, C.
Mouse
Mouse, 6 week
Alveolar epithelial DMEM/F-12
Lewis, K. , Hall, J. , Kiyotake, E. , Christensen, T. , Balasubramaniam, V. and Anseth, K.
Mouse
Mouse, female
Antigen presenting cells RPMI 1640
Mouse
Mouse
Dendritic HBSS
Lancelin, W. and Guerrero-Plata, A.
Mouse
Mouse
Fibroblasts HBSS
Qiu, Y. , Xu, Y. , Wang, M. , Miao, M. , Zhou, H. , Xu, J. , Kong, J. , Zheng, D. , Li, R. , Zhang, R. , Guo, Y. , Li, X. , Cui, J. , Qin, C. and Zhou , X.
Mouse
Mouse
Lung Krebs-Henseleit Buffer
Freedman SD, Katz MH, Parker EM, Laposata M, Urman MY. and Alvarez JG.
Mouse
Mouse, 6-7 week
Leukocytes and endothelial PBS
Jungblut, M. , Oeltze, K. , Zehnter, I. , Hasselmann, D. and Bosio, A.
Mouse
Mouse, embryonic
Avelolar progenitor DMEM/F12
Desai, T. , Brownfield, D. and Krasnow, M.
Mouse
Mouse, female, 18-20 g
Murine endothelial cells DMEM
Dong QG, Bernasconi S, Lostaglio S, De Calmanovici RW, Martin-Padura I, Breviario F, Garlanda C, Ramponi S, Mantovani A,and Vecchi A.
Mouse
Mouse
Pulmonary see reference
Kim, H. , Lee, H. , Chang, Y. , Pichavant, M. , Shore, S. , Fitzgerald, K. , Ikawura, Y. , Israel, E. , Bolger, K. , Faul, J. , DeKruyff, R. and Umetsu, D.
Mouse
Mouse, 6-8 wk
Lung cells HBSS
Stampfli MR, Wiley RE, Neigh GS, Gajewska BU, Lei XF, Snider DP, Xing Z, Jordana M.
Mouse
Mouse, 8-12 week
Lung progenitor DMEM
Driscoll, B. , Kikuchi, A. , Lau, A. , Lee, J. , Reddy, R. , Jesudason, E. , Kim, C. and Warburton, D.
Mouse
Mouse, 8-12 week
Mononuclear cells RPMI 1640 medium
Mouse
Mouse
Lung tumor DMEM
Mouse
Mouse, 18-20 g
Lung HBSS
Ebeling, C. , Lam, T. , Gordon, J. , Hollenberg, M. and Vliagoftis, H.
Mouse
Mouse
Lung RPMI 1640
Hardy, C. , LeMasurier, J. , Belz, G. , Scalzo-Inguanti, K. , Yao, J. , Xiang, S. , Kanellakis, P. , Bobik, A. , Strickland, D. , Rolland, J. , O'Hehir, R. and Plebanski, M.
Mouse
Mouse, embryo, day 18
Alveolar and fibroblast MEM
Mouse
Mouse
Lung DMEM/F12
Rock, J, Barkauskas, C, Cronc, M, Xue, Y, Harris, J, Liang, J, Noble, P. and Hogan, B.
Mouse
Mouse
Lung Dulbecco's PBS
Finotto, S. , Eigenbrod, T. , Karwot, R. , Boross, I. , Doganci, A. , Ito, H. , Nishimoto, N. , Yoshizaki, K. , Kishimoto, T. , Rose-John, S. , Galle, P. and Neurath, M.
Mouse
Mouse
Alveolar HBSS
Mohapatra, A. , Van Dyken, S. , Schneider, C. , Nussbaum, J. , Liang, H. and Locksley, R.
Mouse
Mouse, female, 6-12 week
Lung mononuclear HBSS
Breslow, R. , Rao, J. , Xing, W. , Hong, D. , Barrett, N. and Katz, H.
Mouse
Mouse
Alveolar epithelial DMEM
Mouse
Mouse
Lung RPMI 1640
Porcine
Swine fetuses (70 day), adult Amer Dutch 250 day
(also human fetuses, 80 day)
Lung HBSS
Rabbit
Rabbit, New Zealand white, adult,male
Alveolar type II Joklik's MEM
Finkelstein, J. , Maniscalco, W. and Shapiro, D.
Rabbit
Rabbit, New Zealand, white, male, 1.7 Kg
Lung
  • Pronase : 0.2%
Kreb's serum substitute solution, CMF
Gould, M. , Clements, J. , Jones, A. and Felts, J.
Rabbit
Rabbit, New Zealand, male, 2-3 kg
Clara cells
  • Protease : 0.1%
HEPES
Devereux, T. and Fouts, J.
Rabbit
Rabbit, New Zealand, white
Alveolar type II HBSS
Rat
Rat, SD, male, 250-400 g
Alveolar type II Phosphate-buffered medium
(see reference)
Rat
Rat, SD, male, adult
Alveolar DMEM/Ham's F-12
(see reference)
Rat
Rat, SD, male, 150 - 200 g
Pneumocytes type II BSS
Brown, L. and Longmore, W.
Rat
Rat, embryonic day 15
Fetal alveolar epithelial type II DMEM
Fraslon-Vanhulle, C, Bourbon, J. and Batenburg, J
Culture of Fetal Alveolar Epithelial Type II Cells , Cell & Tissue Culture: Laboratory Procedures Vol. 2 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 13A:2.1 , 1995
Rat
Rat, Wistar, adult, male and pregnant female (known gestation)
Alveolar type II RPMI 1640
Batenburg, J. , Otto-Verberne, C. , Have-Opbroek, A. and Klazinga, W.
Rat
Rat, male
Alveolar epithelial DMEM
Planus, E. , Galiacy, S. , Matthay, M. , Laurent, V. , Gavrilovic, J. , Murphy, G. , Clerici, C. , Isabey, D. , Lafuma, C. and d'Ortho, M.
Rat
Rat, fetus, 19 day
Fibroblasts & type II HBSS: serum free MEM, CMF
Rat
Rat, SD, 250-300g
Alveolar epithelial RPMI 1640
Chen J. , Chen Z. , Narasaraju T. , Jin N. and Liu L.
Rat
Rat, Fischer 344, male
Alveolar type II pneumocytes HEPES buffer
(see reference)
Mangum, J. , Everitt, J. , Bonner, J. , Moore, L. and Brody, A.
Rat
Rat, SD, 300-400 g
Pulmonary endothelial cells DMEM/F-12
King J, Hamil T, Creighton J, Wu S, Bhat P, McDonald F. and Stevens T
Rat
Rat, Wistar
Epithelial HBSS
Jassal, D. , Han, R. , Caniggia, I. , Post, M. and Tanswell, A.
Rat
Rat, SD, 200-225 g
Type II alveolar epithelial cells DMEM
Rat
Rat, adult
Lung Moscona saline, CMF
Douglas, W. and Kaighn, M.
Rat
Rat, Lewis, male, 200-250g
Interstitial lung macrophages RPMI-1640
Rat
Rat, SD, 8 day
Interstitial HEPES buffer
Berk, J. , Franzblau, C. and Goldstein, R.
Rat
Rat, SD, 125-150 g
Alveolar type I & II DMEM/F12
Liebler, J. , Borok, Z. , Li, X. , Zhou, B. , Sandoval, A. and Kim, K.and Crandall, E.
Rat
Rat, SD, male, 100 g
Alveolar type II Joklik's medium
Kikkawa, Y. and Yoneda, K.
Rat
Rat
Lung PBS
Rat
Rat, SD, male/female, 180-250 g
Alveolar type II BSS
Mason, R. , Williams, M. , Greenleaf, R. and Clements, J.
Rat
Rat, fetus, 19 days
Alveolar pneumonocytes, type II HBSS, CMF
Rat
Rat, Wistar, pathogen free
Alveolar type II Earle's MEM
King, R.
Rat
Rat, SD, male, 180-200 g
Alveolar type II HEPES
(see reference)
Dobbs, L. , Gonzalez R. and Williams, M.
Rat
Rat, SD, male, 125-175 g
Alveolar epithelial EBSS
Kim, K. , Suh, D. , Lubman, R. , Danto, S. , Borok, Z. and Crandall, E.
Rat
Rat, male, 200-250g
Pulmonary arterial myocytes PBS
Rat
Rat, Wistar, female, virgin
Alveolar epithelial type II Eagle's MEM
Fraslon, C. , Rolland, G. , Bourbon, J. , Rieutort, M. and Valenza, C.
Rat
Rat, SD, male, 150-400 g
Alveolar type II Auto-Pow Eagle's modified MEM
Goodman, B. , Fleischer, R. and Crandall, E.
Rat
Rat, SD, male
Alveolar type I DMEM
Weller, N. and Karnovksy, M.

Tissue Tables (references, grouped by tissue type and species)