Pancreas

Species	Cells	Enzyme(s)	Medium	Reference
Bovine Bovine (Bos taurus)	Ductal	Neutral Protease : 0.05%	EBSS	Sato, T. , Sato, M. , Hudson, E. and Jones, R. Characterization of Bovine Pancreatic Ductal Cells Isolated by a Perfusion-Digestion Technique , In Vitro 19 , 651 , 1983
Bovine Bovine	Platelets	Trypsin	(see reference)	Stiles, G. and Lefkowitz, R. Hormone-Sensitive Adneylate Cyclase , J Biol Chem 257 (11) , 6287 , 1982
Bovine Bovine	Duct epithelial	Collagenase : 0.1%	HEPES	Cotton, C. and Al-Nakkash, L. Isolation and Culture of Bovine Pancreatic Duct Epithelial cells , Am J Physiol 272 , G1328 , 1997
Canine Canine	Buccal ganglia; SLT muscle	Trypsin : 0.2%	DMEM	Zoran, M. , Doyle, R. and Haydon, P. Target Contact Regulates the Calcium Responsiveness of the Secretory Machinery During Synaptogenesis , Neuron 6 , 145 , 1991
Canine Canine, adult mongrel, 15-25 kg	Islets	Collagenase Type 4 : 600-1100 u/ml Deoxyribonuclease I : 10 ug/ml	RPMI 1640	Noel, J. , Rabinovitch, A. , Olson, L. , Kyriakides, G. , Miller, J. and Mintz, D. A Method for Large-Scale, High-Yield Isolation of Canine Pancreatic Islets of Langerhans , Metabolism 31 (2) , 184 , 1982
Fish Fish, Osphronemus gourami, 3-month-old, 0.5 mg	Islets	Collagenase : 0.12 - 0.46 u/ml	RPMI 1640	Schrezenmeir, J. , Laue, C. , Sternheim,E. , Wolbert, K. , Darquy, S. , Chicheportiche, D. , Kirchgessner, J. and Reach, G. Long-Term Function of Single-Cell Preparations of Piscine Principal Islets in Hollow Fibers , Transplant Proc 24 (6) , 2941 , 1992
Guinea-Pig Guinea-pig	Exocrine	Hyaluronidase : 0.15% - 0.2%	Kreb's Ringer	Amsterdam, J. and Jamieson, J. Structural and Functional Characterization of Isolated Pancreatic Exocrine Cells , Proc Natl Acad Sci U S A 69 (10) , 3028 , 1972
Guinea-Pig Guinea-pig	Acinar	Collagenase Type 3 : 60 u/ml	Kreb's Ringer	Schultz, G. , Sarras, Jr, M. , Gunther, G. , Hull, B. , Alicea, H. , Gorelick, F. and Jamieson, J. Guinea Pig Pancreatic Acini Prepared with Purified collagenase , Exp Cell Res 130 , 49 , 1980
Guinea-Pig Guinea-pig, Hartley, albino, male, 350-400 g	Acinar	Soybean Trypsin Inhibitor : 0.01%	Kreb's Ringer	Gardner, J. , Conlon, T. , Klaeveman, H. , Adams, T. and Ondetti, M. Action of Cholecystokinin and Cholinergic Agents on Calcium Transport in Isolated Pancreatic Acinar Cells , J Clin Invest 56 , 366 , 1975
Hamster Hamster	Islets	Collagenase Type 4 : 1.3% - 2.0%	HBSS	Feldman, J. and Chapman, B. Preparation of Islets of Langerhans from Rabbits and Hamsters by the Collagenase Digestion Technique , Acta Diabetol 12 , 208 , 1975
Human Human	Pancreatic cancer stem cells	Collagenase Type 4 : 200 u/ml	medium 199	Li, C. , Heidt, D. , Dalerba, P. , Burant, C. , Zhang, L. , Adsay, V. , Wicha, M. , Clarke, M. and Simeone, D. Identification of Pancreatic Cancer Stem Cells. , Cancer Res 67 , 1030 , 2007
Human Human	Acinar	CLSPA : 200 u/ml Soybean Trypsin Inhibitor : 0.01%	See Reference	Cane, M. , Sutton, R. and Criddle, D. Isolation of Human Pancreatic Acinar Cells From Rat and Human Pancreas , Pancreapedia 20 , 1 , 2011
Human Human	Islets	Collagenase Type 1 : 1,600u/isolation Neutral Protease : 200 u/isolation Deoxyribonuclease I : 12,000 u/isolation	RPMI	Brissova, M. , Haliyur, R. , Saunders, D. , Shrestha, S. , Dai, C. , Blodgett, D. , Bottino, R. , Campbell-Thompson, M. , Aramandla, R. , Poffenberger, G. , Lindner, J. , Pan, F. , von Herrath, M. , Greiner, D. and Shultz, L. Î± Cell Function and Gene Expression Are Compromised in Type 1 Diabetes. , Cell Rep 22 , 2667-2676 , 2018
Human Human	Islets	Collagenase Type 4 : 0.8%	HBSS	Izumi, R. , Konishi, K. , Ueno, K. , Shimizu, K. , Hirosawa, H. , Takahashi, N. and Miyazaki, I. Isolation of Human Pancreatic Islets from Cryopreserved Pancreas , Transplant Proc XVII , 383 , 1985
Human Human	Islets	Collagenase (1 or 4) : 0.60%	HBSS	Gray, D. , McShane, P. , Grant, A. and Morris, P. A Method for Isolation of Islets of Langerhans from the Human Pancreas , Diabetes 33 , 1055 , 1984
Human Human, 25-55 yrs old (also porcine)	Islets	Collagenase : 0.4%	HBSS	Contractor, H. , Johnson, P. , Chadwick, D. , Robertson, G. and London, N. The Effect of UW Solution and its Components on the Collagenase Digestion of Human and Porcine Pancreas , Cell Transplant 4 , 615 , 1995
Human Human, infant, age 1 day-1 year	Islets	Collagenase : 170-210 u/ml	HBSS	Sutherland, D. , Matas, A. , Steffes, M. and Najarian, J. Infant Human Pancreas: A Potential Source of Islet Tissue for Transplantation , Diabetes 25 (12) , 1123 , 1976
Human Human	Islets	Trypsin : Trypsin: 0.125-0.25% Collagenase : 0.2% Deoxyribonuclease I : 200 u/ml	Eurocollins solution	Watt, P. , Mullen, Y. , Benhamou, P. , Hober, C. , Nomura, Y. , Watanabe, Y. , Passaro, E. , Zinner, M. and Brunicardi, F. Simplified Semiautomated Method For Isolating Islets From the Human Pancreas , Transplant Proc 26 (2) , 582 , 1994
Human Human, adult organ donors	Islets	Collagenase : 0.6%	Eurocollins solution	Warnock, G. , Rajotte, R. , Evans, M. , Ellis, D. , DeGroot, T. and Dawidson, I. Isolation of Islets of Langerhans Following Cold Storage of Human Pancreas , Transplant Proc XIX (4) , 3466 , 1987
Monkey Monkey, 3-5 Kg	Islets	Hyaluronidase : 0.05%	HBSS	Scharp, D. , Murphy, J. , Newton, W. , Ballinger, W. and Lacy, P. Application of an Improved Isolation Technique for Islet Transplantation in Primates and Rats , Transplant Proc 7 , 739 , 1975
Mouse Mouse	Acinar cells and acini	Collagenase Type 1 : see reference CLSPA : see reference	see reference	Toivola, D. , Ku, N. , Ghori, N. , Lowe, A. , Michie, S. and Omary, M. Effects of Keratin Filament Disruption on Exocrine Pancreas-Stimulated Secretion and Susceptibility to Injury. , Exp Cell Res 255 , 156 , 2000
Mouse Mouse, 10 week	Islets	Collagenase : 0.2%	RPMI 1640	Kobayashi, T. , Yamaguchi, T. , Hamanaka, S. , Kato-Itoh, M. , Yamazaki, Y. , Ibata, M. , Sato, H. , Lee, Y. , Usui, J. , Knisely, A. , Hirabayashi, M. and Nakauchi, H. Generation of Rat Pancreas in Mouse by Interspecific Blastocyst Injection of Pluripotent Stem Cells. , Cell 142 , 787 , 2010
Mouse Mouse, 6-10 month	Pancreatic ductal	CLSPA : 50 u/ml Hyaluronidase : 400 u/ml Soybean Trypsin Inhibitor : 0.02%	DMEM	Wang, Y. , Soyombo, A. , Shcheynikov, N. , Zeng, W. , Dorwart, M. , Marino, C. , Thomas, P. and Muallem, S. Slc26a6 Regulates CFTR Activity In Vivo to Determine Pancreatic Duct HCO3- Secretion: Relevance to Cystic Fibrosis. , EMBO J 25 , 5049 , 2006
Mouse Mouse, 7-10 week	Islets	Collagenase : see reference	DMEM	Yesil, P, Michel, M. , Chwalek, K. , Pedack, S. , Jany, C. , Ludwig, B. , Bornstein, S. and Lammert, E. A New Collagenase Blend Increases the Number of Islets Isolated from Mouse Pancreas. , Islets 1 , 185
Mouse Mouse	Acinar	Collagenase : 100 u/ml	HEPES	Jauch, P. , Peterson, O. and Lauger, P. Electrogenic Properties of the Na-Alanine Cotransporter in Acinar Cells , J Membr Biol 94 , 99 , 1986
Mouse Mouse, albino, 25 g	Acinar	CLSPA : see reference	see reference	Fogarty, K. , Kidd, J. , Tuft, R. and Thorn, P. A Bimodal Pattern of InsP(3)-Evoked Elementary Ca(2+) Signals in Pancreatic Acinar Cells. , Biophys J 78 , 2298 , 2000
Mouse Mouse (C57BL/6J-ob/ob), 9-12 wks, male	Islets	Hyaluronidase : 0.5%	Kreb's Ringer bicarbonate buffer	Dalpe-Scott, M. , Heick, H. and Begin-Heick, N. Secretion in the Obese (ob/ob) Mouse. The Effect of Oxytetracycline on Insulin Release , Diabetes 32 , 932 , 1983
Mouse Mouse	Islets	Collagenase : 1,000 u/ml	RPMI 1640	Li, D. , Yuan, Y. , Tu, H. , Liang, Q. and Dai, L. A Protocol for Islet Isolation from Mouse Pancreas. , Nat Protoc 4 , 1649 , 2009
Mouse Mouse, BALB/c, 6-8 week-old, either sex	Acinar	Collagenase : 0.1%	Waymouth's MB	Kurup, S. and Bhonde, R. Analysis and Optimization of Nutritional Set-up for Murine Pancreatic Acinar Cells , JOP 3 (1) , 8 , 2002
Mouse Mouse	Islets	Collagenase : 0.03-0.08%	RPMI 1640	Szot, G. , Koudria, P. and Bluestone, J. Murine Pancreatic Islet Isolation. , J Vis Exp 7 , 255 , 2007
Mouse Mouse	Islets	Collagenase Type 2 : 0.2%	CF Medium	Koster, J. , Marshall, B. , Ensor, N. , Corbett, J. and Nichols, C. Targeted Overativity of Cell KATP Channels Induces Profound Neonatal Diabetes , Cell 100 , 645 , 2000
Mouse Mouse, 11-12 week	Islets	Collagenase Type 4 : 0.2%	RPMI	Astrof, S. , Crowley, D. , George, E. , Fukuda, T. , Sekiguchi, K. , Hanahan, D. and Hynes, R. Direct Test of Potential Roles of EIIIA and EIIIB Alternatively Spliced Segments of Fibronectin in Physiological and Tumor Angiogenesis. , Mol Cell Biol 24 , 8662-70 , 2004
Mouse Mouse	Duct	Papain : 25 u/ml	DMEM /F-12	Githens, S. Pancreatic Duct Epithelial Cells , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12B:12.1 , 1995
Mouse Mouse, 6-8 month	Islets	Collagenase Type 4 : 0.1%	HBSS	Taguchi, Y. , Tasaki, Y. , Terakado, K. , Kobayashi, K. , Machida, T. and Kobayashi, T. Impaired Insulin Secretion from the Pancreatic Islets of Hypothyroidal Growth-Retarded Mice. , J Endocrinol 206 , 195-204 , 2010
Mouse Mouse, male, 18-24 g	Acinar	CLSPA : 70-90 u/ml Soybean Trypsin Inhibitor : 0.01%	Krebs-Henseleit	Burnham DB, Williams JA. Effects of carbachol, cholecystokinin, and insulin on protein phosphorylation in isolated pancreatic acini , J Biol Chem 257 , 10523-8 , 1982
Mouse Mouse	Islets	Collagenase : 0.1-0.25%	HBSS	Koh, D. , Moody, M. and Jo, J. Collection of Islets of Langerhans using an Equilibrium Method. , Biotechniques 55 , 34-7 , 2013
Mouse Mouse	Pancreatic islet	Collagenase Type 4 : 0.2%	HBSS	Haefliger Jacques-Antoine, Tawadros Thomas, Meylan Laure, Gurun SabineLe, Roehrich Marc-Estienne, Martin David, Thorens Bernard, Waeber Gerard. The scaffold protein IB1/JIP-1 is a critical mediator of cytokine-induced apoptosis in pancreatic beta cells , J Cell Sci 116 , 1463-9 , 2003
Mouse Mouse, 8-12 week	Pancreas organoid	Neutral Protease : 0.012% Collagenase : 0.012%	DMEM	Huch, M. , Bonfanti, P. , Boj, S. , Sato, T. , Loomans, C. , Van de Wetering, M. , Sojoodi, M. , Li, V. , Schuijers, J. , Gracanin, A. , Ringnalda, F. , Begthel, H. , Hamer, K. , Mulder, J. , Van Es, J. and De Koning, E. Unlimited In Vitro Expansion of Adult Bi-Potent Pancreas Progenitors Through the Lgr5/R-Spondin Axis. , EMBO J 32 , 2708-21 , 2013
Mouse Mouse	Pancreatic islets	Collagenase Type 4 : 0.4%	Gey's BSS	Strowski M, Parmar R, Blake A, Schaeffer J. Somatostatin inhibits insulin and glucagon secretion via two receptors subtypes: an in vitro study of pancreatic islets from somatostatin receptor 2 knockout mice , Endocrinology 141 , 111-7 , 2000
Mouse Mouse, embryonic	Pancreatic progenitor	Neutral Protease : 0.125%	DMEM	Greggio, C. , De Franceschi, F. , Figueiredo-Larsen, M. , Gobaa, S. , Ranga, A. , Semb, H. , Lutolf, M. and Grapin-Botton, A. Artificial Three-Dimensional Niches Deconstruct Pancreas Development In Vitro. , Development 140 , 4452-62 , 2013
Mouse Mouse	Islets	Collagenase Type 4 : 0.2%	HBSS	Wu Yulian, Han Bing, Luo Hongyu, Roduit Raphael, Salcedo TheodoraW, Moore PaulA, Zhang Jun, Wu Jiangping. DcR3/TR6 effectively prevents islet primary nonfunction after transplantation , Diabetes 52 , 2279-86 , 2003
Mouse Mouse	Islets	Collagenase : 0.14%	RPMI 1640	Carter, J. , Dula, S. , Corbin, K. , Wu, R. and Nunemaker, C. A Practical Guide to Rodent Islet Isolation and Assessment. , Biol Proced Online 11 , 3 , 2009
Mouse Mouse, adult, male	Ancinar	CLSPA : see reference Soybean Trypsin Inhibitor : 0.001%	DMEM	Ji, B. , Gaiser, S. , Chen, X. , Ernst, S. and Logsdon, C. Intracellular Trypsin Induces Pancreatic Acinar Cell Death but not NF-KappaB Activation. , J Biol Chem 284 , 17488 , 2009
Mouse Mouse	Islets	Collagenase : 0.2%	RPMI 1640	Bertera, S. , Balamurugan, A. , Bottino, R. , He, J. and Trucco, M. Increased Yield and Improved Transplantation Outcome of Mouse Islets with Bovine Serum Albumin. , J Transplant 2012 , 856386 , 2012
Mouse Mouse, 3-4 week, 20-24 g	Islets	Collagenase Type 4 : 0.2%	RPMI 1540	Huang, H. , Xie, Q. , Kang, M. , Zhang, B. , Zhang, H. , Chen, J. , Zhai, C. , Yang, D. , Jiang, B. and Wu, Y. Labeling Transplanted Mice Islet with Polyvinylpyrrolidone Coated Superparamagnetic Iron Oxide Nanoparticles for In Vivo Detection By Magnetic Resonance Imaging. , Nanotechnology 20 , 365101 , 2009
Mouse Mouse	Islet	Collagenase Type 4 : 0.2%	RPMI-1640	Ding, G. , Wang, F. , Shu, J. , Tian, S. , Jiang, Y. , Zhang, D. , Wang, N. , Luo, Q. , Zhang, Y. , Jin, F. , Leung, P. , Sheng, J. and Huang, H. Transgenerational Glucose Intolerance with Igf2/H19 Epigenetic Alterations in Mouse Islet Induced by Intrauterine Hyperglycemia. , Diabetes 61 , 1133-42 , 2012
Mouse Mouse, adult	Acinar	CLSPA : 200 u/ml	see reference	Voronina, S. , Barrow, S. , Gerasimenko, O. , Petersen, O. and Tepikin, A. Effects of Secretagogues and Bile Acids on Mitochondrial Membrane Potential of Pancreatic Acinar Cells: Comparison of Different Modes of Evaluating Delta Psi. , J Biol Chem 279 , 27327 , 2004
Mouse Mouse	Islets	Collagenase Type 4 : 0.1%	HBSS	Low, L. , Zavortink, M. , Mitchell, J. , Gan, W. , Do, O. , Schwiening, C. , Gaisano, H. and Thorn, P. Insulin Secretion from Beta Cells in Intact Mouse Islets is Targeted Towards the Vasculature , Diabetologia 57 , 1655 , 2014
Porcine Porcine, female	Islets	Collagenase : 0.1%	HBSS	Brandhorst, D. , Brandhorst, H. , Hering, B. , Federlin, K. and Bretzel, R. Islet Isolation from the Pancreas of Large Mammals and Humans: 10 Years of Experience , Exp Clin Endocrinol 103 , 3 , 1995
Porcine Porcine, 10-36 months, 200-300 kg	Islets	Collagenase : 0.2%	HBSS	Ricordi, C. , Socci, C. , Davalli, A. , Staudacher, C. , Baro, P. , Vertova, A. , Sassi, I. , Gavazzi, F. , Pozza, G. and Di Carlo, V. Isolation of the Elusive Pig Islet , Surgery 107 (6) , 688 , 1990
Porcine Porcine, <2 year, 200-250 kg	Islets	Collagenase : 0.1%	HBSS	Heiser, A. , Ulrichs, K. and Muller-Ruchholtz, W. Isolation of Porcine Pancreatic Islets: Low Trypsin Activity During the Isolation Procedure Guarantees Reproducible High Islet Yields , J Clin Lab Anal 8 , 407 , 1994
Porcine Porcine	Islets	Collagenase : 0.1%-0.2%	HBSS	Johnson, P. , van Suylichem, P. , Roberts, D. , Vos-Scheperkeuter, G. , White, S. , van Schlifgaarde, R. and London, N. Design of a Simple, in vitro Method for Evaluation of the Efficiency of Crude Clostridium histolyticum Collagenase and its Components for Porcine Islet Isolation , Xenotransplantation 2 , 165 , 1995
Porcine Porcine, 1-3 year, 1.5-2.0 kg, either sex	Islets	Collagenase : 0.25%	HBSS	Korbutt, G. , Elliott, J. , Ao, Z. , Smith, D. , Warnock, G. and Rajotte, R. Large Scale Isolation, Growth, and Function of Porcine Neonatal Islet Cells , J Clin Invest 97 (9) , 2119 , 1996
Porcine Porcine	Acinar	Collagenase : 100 u/ml	Saline	Iwatsuki, N. and Peterson, O. Action of Tetraethylammonium on Calcium-Activated Potassim Channels in Pig Pancreatic Acinar Cells Studied by Patch-Clamp Single-Channel and Whole-Cell Current Recording , J Membr Biol 86 , 139 , 1985
Porcine Porcine, juvenile	Islets	Collagenase : see reference	Univ of Wisconsin solution	Van der Burg Michael P M, Graham John M. Iodixanol Density Gradient Preparation in University of Wisconsin solution for porcine islet purification , ScientificWorldJournal 3 , 1154-9 , 2003
Porcine Porcine, 3 month, 15-20 kg	Acinar	Collagenase Type 3 : 200 u/ml	RPMI 1640	Zhao, X. , Han, J. and Tang, C. Primary Culture of Porcine Pancreatic Acinar Cells , JOP 2 (2) , 78 , 2001
Rabbit Rabbit, New Zealand white, Male/Female, 2-3 Kg	Acinar	Hyaluronidase : 0.2%	Kreb's Ringer bicarbonate buffer	Renckens, B. , Schrijen, J. , Swarts, H. , DePont, J. and Bonting, S. Role of Calcium in Exocrine Pancreatic Secretion. IV. Calcium Movements in Isolated Acinar Cells of Rabbit Pancreas , Biochim Biophys Acta 544 , 338 , 1978
Rabbit Rabbit, also hamster	Islets	Collagenase Type 4 : 1.3% - 2.0%	HBSS	Feldman, J. and Chapman, B. Preparation of Islets of Langerhans from Rabbits and Hamsters by the Collagenase Digestion Technique , Acta Diabetol 12 , 208 , 1975
Rat Rat, SD, 125 - 350 g	Duct	Trypsin : 0.01%	HBSS	Githens, S. , Holmquist, D. , Whelan, J. and Ruby, J. Ducts of the Rat Pancreas in Agarose Matrix Culture , In Vitro 16 , 797 , 1980
Rat Rat	Exocrine	Hyaluronidase : 0.15%	Krebs	Kondo, S. and Schulz, I. Calcium Ion Uptake in Isolated Pancreas Cells Induced by Secretagogues , Biochim Biophys Acta 419 , 76 , 1976
Rat Rat, adult	Islets	Collagenase Type 1 : 450 u/ml	Leibowitz L-15	Williams, J. , Huang, H. , Kover, K. , Moore, W. , Berkland, C. , Singh, M. , Smirnova, I. , MacGregor, R. and Stehno-Bittel, L. Reduction of Diffusion Barriers in Isolated Rat Islets Improves Survival, but not Insulin Secretion or Transplantation Outcome. , Organogenesis 6 , 115 , 2010
Rat Rat, Wistar, male	Parotid acinar	Trypsin : 0.02%	Solution B (See reference)	Foskett, J. , Roifman, C. and Wong, D. Activation of Calcium Oscillations by Thapsigargin in Parotid Acinar Cells , J Biol Chem 266 , 2778 , 1991
Rat Rat	Exocrine	Hyaluronidase : 0.9%	Kreb's Ringer	Schulz, I. , Heil, K. , Kribben, A. , Sachs, G. and Haase, W. Isolation and Functional Characterization of Cells From the Exocrine Pancreas , Biology of Normal and Cancerous Exocrine Pancreatic Cells , Ribet, A., Pradayrol, L., and Susini, C. , Elsevier , 1980
Rat Rat, SD, adult	Acinar	Collagenase : 0.01% Soybean Trypsin Inhibitor : 0.001%	Culture media	Li, J. , Zhou, R. , Bie, B. , Huang, N. , Guo, Y. , Chen, H. , Shi, M. , Yang, J. , Zhang, J. and Li, Z. Emodin and Baicalein Inhibit Sodium Taurocholate-Induced Vacuole Formation in Pancreatic Acinar Cells. , World J Gastroenterol 24 , 35-45 , 2018
Rat Rat, Wistar, male, 200 - 300 g	Islets	Collagenase Type 4 : 0.5%	HBSS	Shibata, A. , Ludvigsen, C. , Naber, S. , McKaneil M. and Lacy, P. Standardization of a Digestion-Filtration Method for Isolation of Islets , Diabetes 25 , 667 , 1976
Rat Rat	Duct	Papain : 25 u/ml	DMEM /F-12	Githens, S. Pancreatic Duct Epithelial Cells , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12B:12.1 , 1995
Rat Rat, neonate	Islets	Trypsin : 0.05%	Puck's saline buffered w/ EDTA 0.02%	Leonard, R. , Lazarow, A. and Hegre, O. Pancreatic Islet Transplantation in the Rat , Diabetes 22 , 413 , 1973
Rat Rat, SD, male, 150 - 200 g	Acinar	Soybean Trypsin Inhibitor : 0.01%	HEPES	Menozzi, D. , Sato, S. , Jensen, R. and Gardner, J. Cyclic GMP Does Not Inhibit Protein Kinase C-Mediated Enzyme Secretion in Rat Pancreatic Acini , J Biol Chem 264 , 995 , 1989
Rat Rat, Wistar, albino, male, 400 - 500 g	Islets	Collagenase Type 4 : 1.0% - 1.2%	HBSS	Lacy, P. and Kostianovsky, M. Method for the Isolation of Intact Islets of Langerhans from the Rat Pancreas , Diabetes 16 , 35 , 1967
Rat Rat	Ascites hepatoma	Trypsin : 0.1%	Phosphate buffer (see reference)	Essner, E. Experiments on an Ascites Hepatoma. I. Enzymatic Digestion and Alkaline Degradation of the Cementing Substance and Separation of Cells, in Tumor Islands , Exp Cell Res 7 , 430 , 1954
Rat Rat, SD, male, 50-75 g	Acinar	Hyaluronidase : 0.1%	HBSS (see reference)	Brannon, P. , Orrison, B. and Kretchmer, N. Primary Cultures of Rat Pancreatic Acinar Cells in Serum-Free Medium , In Vitro Cell Dev Biol 21 (1) , 6 , 1985
Rat Rat, Wistar, male, 250-400g	Pancreatic islets	Collagenase : 0.75%	RPMI 1640	Tian XH, Xue WJ, Ding XM, Pang XL, Teng Y, Tian PX. and Feng XS Small intestinal submucosa improves islet survival and function during in vitro culture , World J Gastroenterol 11 , 7378 , 2005
Rat Rat, Dark Agouti and Lewis rat donors, adult	Islets	Collagenase Type 1 : 0.3%	Hank's solution	Sutton, R. , Peters, M. , McShane, P. , Gray, D. and Morris, P. An Improved Method for the Isolation of Islets of Langerhans From the Adult Rat Pancreas , Transplant Proc XVII (6) , 1819 , 1986
Rat Rat	Pancreatic acini	CLSPA : 30 u/ml Collagenase Type 4 : 30 u/ml Soybean Trypsin Inhibitor : 0.01%	M199	Blinman TA, Gukovsky I, Mouria M, Zaninovic V, Livingston E, Pandol SJ, Gukovskaya AS. Activation of pancreatic acinar cells on isolation from tissue: cytokine upregulation via p38 MAP kinase , Am J Physiol Cell Physiol 279 , C1993-2003 , 2000
Rat Rat, SD, both sexes, 6-20 wks old	Interlobular ducts	Papain : 25 u/ml	DMEM/Ham's F-12	Githens, S. , Schexnayder, J. , Desai, K. and Patke, C. Rat Pancreatic Interlobular Duct Epithelium: Isolation and Culture in Collagen Gel , In Vitro Cell Dev Biol 25 (8) , 679 , 1989
Rat Rat, 250-350 g	Pancreatic islet	Collagenase Type 4 : 0.2%	HBSS	Haefliger Jacques-Antoine, Tawadros Thomas, Meylan Laure, Gurun SabineLe, Roehrich Marc-Estienne, Martin David, Thorens Bernard, Waeber Gerard. The scaffold protein IB1/JIP-1 is a critical mediator of cytokine-induced apoptosis in pancreatic beta cells , J Cell Sci 116 , 1463-9 , 2003
Rat Rat, Wistar, male	Islets	Collagenase Type 4 : 1%	Medium 199	Katada, T. and Ui, M. Enhanced Insulin Secretion and Cyclic Amp Accumulation in Pancreatic Islets Due to Activation of Native Calcium Ionophores , J Biol Chem 254 (2) , 469 , 1979
Rat Rat, SD, male	Ancinar	CLSPA : see reference Soybean Trypsin Inhibitor : 0.01%	DMEM	Ji B, Kopin AS, Logsdon CD. Species differences between rat and mouse CCKA receptors determine the divergent acinar cell response to the cholecystokinin analog JMV-180 , J Biol Chem 275 , 19115-20 , 2000
Rat Rat, Wistar-Lewis, fetal 18-20 day gestation (also neonatal 3-12 day old)	Islets	Collagenase Type 4 : 0.63%	EBSS (see reference)	Braaten, J. , Jarlfors, U. , Smith, D. and Mintz, D. Purification of Monolayer Cell Cultures of the Endocrine Pancreas , Tissue Cell 7 (4) , 747 , 1975
Rat Rat, 200-350 g	Islets	Collagenase Type 4 : 0.2%	HBSS	Gotoh, M. , Maki, T. , Satomi, S. , Porter, J. , Bonner-Weir, S. , O'Hara, C. and Monaco, A. Reproducible High Yield of Rat Islets By Stationary In Vitro Digestion Following Pancreatic Ductal or Portal Venous Collagenase Injection , Transplantation 43 (5) , 725 , 1987
Rat Rat, SD, male, 250 - 350 g (also mouse, white, Swiss, male, 20 - 24 g)	Acinar	Hyaluronidase : 0.18%	Kreb's Henseleit bicarbonate buffer	Williams, J. , Korc, M. and Dormer, R. Action of Secretagogues on a New Preparation of Functionally Intact, Isolated Pancreatic Acini , Am J Physiol 235 , 517 , 1978
Rat Rat, Fischer-344, either sex, 120-150 g	Epithelial	Trypsin : 0.1%	Ham's F-12/HBSS (see reference)	Tsao, M. and Duguid, W. Establishment of Propagable Epithelial Cell Lines From Normal Adult Rat Pancreas , Exp Cell Res 168 , 365 , 1987
Rat Rat, Wistar, male, 300-350 g	Islets	Collagenase : 0.2%	Kreb's Ringer bicarbonate buffer	Wolters, G. , Konijenendijk, W. and Bouman, P. Effects of Fasting on Insulin Secretion, Islet Glucose Metabolism, and the Cyclic Adenosine 3'5'-Monophosphate Content of Rat Pancreatic Islets In Vitro , Diabetes 26 (6) , 530 , 1977
Rat Rat, Wistar, male, 250 - 350 g	Acinar, parotid	Hyaluronidase : 0.015%	Earle's MEM	Melvin, J. , Kawaguchi, M. , Baum, B. and Turner, R. A Muscarinic Agonist-Stimulated Chloride Efflux Pathway is Associated With Fluid Secretion in Rat Parotid Acinar Cells , Biochem Biophys Res Commun 145 , 754 , 1987
Rat Rat, S-Wistar, male, 230 - 270 g (also mouse, 6 - 8 wk old)	Islets	Collagenase : 0.1% - 0.2%	HBSS	Ohzato, H. , Gotoh, M. , Monden, M. , Dono, K. , Kanai, T. and Mori, T. Improvement in the Islet Yield From a Cold-Preserved Pancreas by Pancreatic Ductal Collagenase Distention at the Time of Harvesting , Transplantation 51 , 566 , 1991
Rat Rat, SD, albino, male, 150-200 g	Acinar, parotid	Trypsin : 0.01% Collagenase : 40-50 u/ml Hyaluronidase : 0.10%	HBSS CMF	Mangos, J. , McSherry, N. , Butcher, F. , Irwin, K. and Barber, T. Dispersed Rat Parotid Acinar Cells (Morphological, Functional Character) , Am J Physiol 229 (3) , 560 , 1975
Rat Rat, SD, male, 42 - 48 day, 175 - 200 g	Acinar, submandibular gland	Hyaluronidase : 0.1 %	HBSS, CF	Quissell, D. , Redman, R. and Mark, M. Short-Term Primary Culture of Acinar-Intercalated Duct Complexes From Rat Submandibular Glands , In Vitro Cell Dev Biol 22 , 469 , 1986
Rat Rat, male, albino, 200-300 g	Islets	Collagenase : 0.5%	Hanks solution	Lacy, P. , Walker, M. and Fink, J. Perifusion of Isolated Rat Islets in Vitro , Diabetes 21 (10) , 987 , 1972
Rat Rat, male, 7-11 week	Islets	Collagenase Type 4 : see reference	RPMI 1640	Getty-Kaushik, L. , Richard, A. , Deeney, J. , Shirihai, O. and Corkey, B. The CB1 Antagonist Rimonabant Decreases Insulin Hypersecretion in Rat Pancreatic Islets , Obesity 17 , 1856 , 2009
Rat Rat, SD, male, 50 - 125 g	Acinar Exorbital lacrimal, parotid, pancreas	Trypsin : 0.01%	HBSS, CMF	Oliver, C. , Waters, J. , Tolbert, C. and Kleinman, H. Growth of Exocrine Acinar Cells on a Reconstituted Basement Membrane Gel , In Vitro Cell Dev Biol 23 , 465 , 1987
Rat Rat	Islets	Collagenase : 126 - 196 u/ml	HBSS	Verspohl, E. and Wienecke, A. The Role of Protein Kinase C in the Desensitization of Rat Pancreatic Islets to Chrolinergic stimulation , J Endocrinol 159 , 287 , 1998
Rat Rat, Wistar, 300g	Islets	Collagenase Type 4 : 0.2%	CMRL 1066	Verga Falzacappa, C. , Mangialardo, C. , Raffa, S. , Mancuso, A. , Piergrossi, P. , Moriggi, G. , Piro, S. , Stigliano, A. , Torrisi, M. , Brunetti, E. , Toscano, V. and Misiti, S. The Thyroid Hormone T3 Improves Function and Survival of Rat Pancreatic Islets During In Vitro Culture. , Islets 2 , 96 , 2010
Rat Rat, SD, male, 40 - 100 g	Acinar	Hyaluronidase : 462 u/ml	Ham's F12	Hirschi, K. , Kenny, S. , Justice, J. and Brannon, P. Effects of Secretin And Caerulein On Enzymes Of Cultured Pancreatic Acinar Cells , In Vitro Cell Dev Biol 27 , 660 , 1991
Rat Rat	Islets	Collagenase : 0.5 - 0.9%	HBSS	Takaki, R. and Ono J Culture of Pancreatic Islet Cells , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12B:11.1 , 1995
Rat Rat	Acinar	CLSPA : 100 u/ml	DMEM	Williams, J. Isolation of Rodent Pancreatic Acinar Cells and Acini by Collagenase Digestion , Pancreapedia , 2010
Rat Rat, SD, male, 100 - 150 g	Parotid acinar	Trypsin : 0.001%	F12 medium	Yeh, C. , Mertz, P. , Oliver, C. , Baum, B. and Kousvelari, E. Cellular Characteristics of Long-Term Cultured Rat Parotid Acinar Cells , In Vitro Cell Dev Biol 27 , 707 , 1991
Rat Rat, Lewis, inbred	Islets	Collagenase : 0.5%	HBSS	Ballinger, W. and Lacy, P. Transplantation of Intact Pancreatic Islets in Rats , Surgery 72 (2) , 175 , 1972
Rat Rat	Islets	Collagenase Type 1 : 450 u/ml	RPMI 1640	MacGregor, R. , Williams, S. , Tong, P. , Kover, K. , Moore, W. and Stehno-Bittel, L. Small Rat Islets are Superior to Large Islets in In Vitro Function and in Transplantation Outcomes. , Am J Physiol/Endo 290 , E771 , 2006