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Tissue References Search Tool

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Kidney
Rat
Rat, SD, 1 week
Valencia L. , Bidet M. , Martial S. , Sanchez E. , Melendez E. , Tauc M. , Poujeol C. , Martin D. , Namorado M. , Reyes J. and Poujeol P.
Nifedipine-activated Ca(2+) Permeability in Newborn Rat Cortical Collecting Duct Cells in Primary Culture ,
Am J Physiol Cell Physiol
 280(5) , C1193 , 2001
Cells
Cortical collection duct
Enzymes
Medium
DMEM/Ham's F12
Kidney
Rat
Rat, SD, 250-300 g
Li, N. , Teggatz, E. , Li, P. , Allaire, R. and Zou, A.
Formation and Actions of Cyclic ADP-Ribose in Renal Microvessels ,
Microvasc Res
 60 , 149 , 2000
Cells
Microvessels
Enzymes
Medium
PSS
Kidney
Rat
Rat, SD, 250-300 g
Mattson D. and Wu F.
Nitric Oxide Synthase Activity and Isoforms in Rat Renal Vasculature ,
Hypertension
 35 , 337 , 2000
Cells
Renal
Enzymes
Medium
see reference
Kidney
Rat
Rat, SD, male, 5-6 week
Miyata, N. , Park, F. , Li, X. and Cowley, A.
Distribution of Angiotensin AT1 and AT2 Receptor Subtypes in the Rat Kidney ,
Am J Physiol
 277 , F437 , 1999
Cells
Renal tubules
Enzymes
Collagenase : 0.1%
Medium
see reference
Kidney
Rat
Rat, SD, 225-250 g
Amiri F, Garcia R.
Regulation of angiotensin II receptors and PKC isoforms by glucose in rat mesangial cells ,
Am J Physiol
 276 , F691-9 , 1999
Cells
Glomerular mesangial cells
Enzymes
Collagenase Type 1 : 250 u/ml
Medium
DMEM
Kidney
Rat
Rat, SD, male
Eitle E, Hiranyachattada S, Wang H, Harris PJ.
Inhibition of proximal tubular fluid absorption by nitric oxide and atrial natriuretic peptide in rat kidney ,
Am J Physiol
 274 , C1075-80 , 1998
Cells
Proximal tubule suspensions
Enzymes
Pronase E : 2 u/ml
Medium
HEPES buffer
Kidney
Rat
Rat, SD, male
Schafer, J. , Watkins, M. , Li, L. , Herter, P. , Haxelmans, S. and Schlatter, E.
A Simplified Method for Isolation of Large Numbers of Defined Nephron Segments ,
Am J Physiol
 273 , F650 , 1997
Cells
Nephron segments
Enzymes
Medium
Eagle's MEM
Kidney
Rat
Rat, 12-weeks-old
Ishikawa, S. , Kusaka, I. , Higashiyama, M. , Nagasaka, S. , Saito, T. , Honda, K. and Saito, T.
Cellular Signaling and Proliferative Action of AVP in Mesangium of SHR: Effect of Low Density Lipoprotein ,
Nat Neurosci
 50 , 1506 , 1996
Cells
Renal
Enzymes
Collagenase : 0.1%
Medium
DMEM
Kidney
Rat
Rat, male, 150-200 g
Elliget, K. and Trump, B.
Primary Cultures of Normal Rat Kidney Proximal Tubule Epithelial Cells for Studies of Renal Cell Injury ,
In Vitro Cell Dev Biol
 27 , 739 , 1991
Cells
Epithelial
Proximal tubule
Enzymes
Protease : 0.1%
Medium
HBSS/CMF
Kidney
Rat
Rat, adult
Brion, L. , Schwartz, J. , Lachman, H. , Zavilowitz, B. and Schwartz, G.
Development of H+ Secretion by Cultured Renal Inner Medullary Collecting Duct Cells ,
Am J Physiol
 257 , F486 , 1989
Cells
Inner medullary collecting duct
Papillae
Enzymes
Medium
PBS
Kidney
Rat
Rat, Wistar, female, 100-150 g
Wang, J. , Kester, M. and Dunn, M.
The Effects of Endotoxin on Platelet-Activating Factor Synthesis in Cultured Rat Glomerular Mesangila Cells ,
Biochim Biophys Acta
 969 , 217 , 1988
Cells
Glomerular mesangial
Enzymes
Collagenase : 0.025%
Medium
RPMI 1640
Kidney
Rat
Rat, Wistar, male, 150 - 200 g
Barlet-Bas, C. , Khadouri, C. , Marsey, S. and Doucet, A.
Sodium-Independent In Vitro Induction of Na+,K+-ATPase by Aldosterone in Renal Target Cells: Permissive Effect of Triiodothyronine ,
Proc Natl Acad Sci U S A
 85 , 1707 , 1988
Cells
Renal target
Enzymes
Collagenase : 1.0% (also 0.7%)
Medium
Eagle's MEM
Kidney
Rat
Rat, Wistar, male, adult
Gesek, F. , Wolff, D. and Strandhov, J.
Improved Separation Method for Rat Proximal and Distal Renal Tubules ,
Am J Physiol
 253 , F358 , 1987
Cells
Proximal tubules
Enzymes
Collagenase : 0.2%
Medium
Krebs-Henseleit buffer
Kidney
Rat
Rat,Wistar, 300-400 gm
Vinay, P. , Gougoux, A. and Lemieux, G.
Isolation of a Pure Suspension of Rat Proximal Tubules ,
Am J Physiol
 241 , F403 , 1981
Cells
Proximal tubules
Enzymes
Collagenase : 0.15 %
Medium
Krebs Henseleit solution
Liver
Avian
Avian, various
Else, P. , Brand, M. , Turner, N. and Hulbert, A
Respiration Rate of Hepatocytes Varies With Body Mass in Birds ,
J Exp Biol
 207 , 2305 , 2004
Cells
Hepatocytes
Enzymes
Medium
see reference
Liver
Avian
Ducklings, 7 day
Lee J. , Culvenor J. , Angus P. , Smallwood R. , Nicoll A. and Locarnini S.
Duck Hepatitis B Virus Replication in Primary Bile Duct Epithelial Cells ,
J Virol
 75(16) , 7651 , 2001
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Hyaluronidase : 0.05%
Medium
DMEM/ F12
Liver
Bovine

Bovine, Holstein, 22-23 month

Testroet, E. , Choudhary, S. , Choudhary, R. , Beitz, D. and Du, M.
Tumor Necrosis Factor Alpha and Palmitate Simulate Bovine Fatty Liver Disease in vitro when Using Abattoir-Derived Primary Bovine Hepatocytes Isolated by a Novel Nonperfusion Method. , JDS Commun Vol. 3 , 456-461 , 2022
Cells
Hepatocytes
Enzymes
Collagenase Type 2 : 200 u/ml
Medium
DMEM
Liver
Canine
Dog, 13 years old
(also rat, guinea-pig, rabbit, human)
Reese, J. and Byard, J.
Isolation And Culture of Adult Hepatocytes from Liver Biopsies ,
In Vitro
 17 , 935 , 1981
Cells
Hepatocytes
Enzymes
Collagenase : 90 u/ml
Medium
CF EGTA perfusate
Liver
Chicken
Chicken, adult
Fraslin, J. , Touquette, L. , Douaire, M. , Menezo, Y. , Guillemot, J. and Mallard, J.
Isolation and Long Term Maintenance of Differentiated Adult Chicken Hepatocytes in Primary Culture ,
In Vitro Cell Dev Biol
 28 , 615 , 1992
Cells
Hepatocytes
Enzymes
Collagenase : 0.02%
Medium
HEPES, CF
Liver
Chicken
Chicken
(also rat, SD, 150-250 g)
Weigel, P. , Schnaar, R. , Kuhlenschmidt, M. , Schmell, E. , Lee, R. , Lee, Y. and Roseman, S.
Adhesion of Hepatocytes to Immobilized Sugars ,
J Biol Chem
 254 (21) , 10830 , 1979
Cells
Hepatocytes
Enzymes
Collagenase Type 4 : 6000 units
Medium
HEPES
Liver
Equine
Equine, 4-13 yr
Bakala A. , Karlik W. and Wiechetek M.
Preparation of Equine Isolated Hepatocytes ,
Toxicol In Vitro
 17(5-6) , 615 , 2003
Cells
Hepatocytes
Enzymes
Medium
HBSS
Liver
Fish
Rainbow trout (Oncorhynchus mykiss), male & female,
120-600 g
Blair, J.B. , Miller, M.R. , Pack, D. , Barnes, R. , Teh, S.J. and Hinton, D.E.
Isolated Trout Liver Cells:Establishing Short-Term Primary Cultures Exhibiting Cell-to-Cell Interactions ,
In Vitro Cell Dev Biol
 26 , 237 , 1990
Cells
Hepatocytes
Enzymes
Medium
Kreb's-Ringer bicarbonate buffer, CF
Liver
Fish
Trout, male, 100 - 200 g
Lipsky, M. , Sheridan, T. , Bennett, R. and May, E.
Comparison of Trout Hepatocyte Culture on Different Substrates ,
In Vitro Cell Dev Biol
 22 , 360 , 1986
Cells
Hepatocytes
Enzymes
Medium
HBSS with 0.05M HEPES
Liver
Fish
Rainbow trout, (Salmo gairdneri), male, 150-200 g
Klaunig, J. , Ruch, R. and Goldblah, P.
Trout Hepatocyte Culture: Isolation and Primary Culture ,
In Vitro Cell Dev Biol
 21 , 221 , 1985
Cells
Hepatocytes
Enzymes
Collagenase : 100 u/ml
Medium
HBSS/CMF
Liver
Fish
Trout (Salmo gairdneri), male, 150-200 g
Catfish (Ictalurus punctatus), male, 100 - 150 g
Klaunig, J.
Establishment of Fish Hepatocyte Cultures For Use in In Vitro Carcinogenicity Studies ,
Natl Cancer Inst Monogr
 65 , 163 , 1981
Cells
Hepatocytes
Enzymes
Collagenase : 100u/ml
Medium
HBSS
Liver
Guinea-Pig
Guinea-pig, Hartley, male, 250-300 g
Arinze, I. and Kawai, Y.
Adrenergic Regulation of Glycogenolysis in Isolated Guinea-Pig Hepatocytes: Evidence that B2-Receptors Mediate Catecholamine Stimulation of Glycogenolysis ,
Arch Biochem Biophys
 225 , 196 , 1983
Cells
Hepatocytes
Medium
Kreb's Ringer bicarbonate buffer
Liver
Guinea-Pig
Guinea-pig, young
Reese, J. and Byard, J.
Isolation And Culture of Adult Hepatocytes from Liver Biopsies ,
In Vitro
 17 , 935 , 1981
Cells
Hepatocytes
Enzymes
Collagenase : 90 u/ml
Medium
CF EGTA perfusate
Liver
Human

Human

Kucykowicz, S. , Amin, O. , Burton, A. , Swadling, L. , Schmidt, N. , Zakeri, N. , Davies, J. , Aidoo-Micah, G. , Stegmann, K. , Easom, N. , Jeffery-Smith, A. , Maini, M. and Pallett, L.
Isolation of Human Intrahepatic Leukocytes for Phenotypic and Functional Characterization by Flow Cytometry. ,
Star Protocols
 3 , 101356 , 2022
Cells
Intrahepatic leukocytes
Enzymes
Medium
RPMI-1640
Liver
Human
Human
Reuschel, E, Jilg, W. , Seelbach-Goebel, B. and Demi, L.
Comparative Purification and Characterization of Hepatitis B Virus-like Particles Produced by Recombinant Vaccinia Viruses in Human Hepatoma Cells and Human Primary Hepatocytes ,
PLoS ONE
 , 2019
Cells
Hepatocytes
Enzymes
Medium
HBSS
Liver
Human
Human
Pfeiffer, E. , Kegel, V. , Zeilinger, K. , Hengstler, J. , Nussler, A. , Seehofer, D. and Damm, G.
Featured Article: Isolation, Characterization, and Cultivation of Human Hepatocytes and Non-Parenchymal Liver Cells. ,
Exp Biol Med (Maywood)
 240 , 645-56 , 2015
Cells
Hepatocytes and non-parenchymal cells
Enzymes
Medium
HBSS
Liver
Human
Human
Werner, M. , Driftmann, S. , Kleinehr, K. , Kaiser, G. , Mathe, Z. , Treckmann, J. , Paul, A. , Skibbe, K. , Timm, J. , Canbay, A. , Gerken, G. , Schlaak, J. and Broering, R.
All-In-One: Advanced Preparation of Human Parenchymal and Non-Parenchymal Liver Cells. ,
PLoS ONE
 10 , e0138655 , 2015
Cells
Hepatocytes
Enzymes
Medium
DMEM
Liver
Human
Human
Lee, S. , Schelcher, C. , Demmel, M. , Hauner, M. and Thasler, W.
Isolation of Human Hepatocytes by a Two-Step Collagenase Perfusion Procedure. ,
J Vis Exp
 , 2013
Cells
Hepatocytes
Enzymes
Collagenase : 0.1-0.15%
Medium
HEPES
Liver
Human
Human, fetal
Vatakis, D. , Bristol, G. , Kim, S. , Levin, B. , Liu, W. , Radu, C. , Kitchen, S. and Zack, J.
Using the BLT Humanized Mouse as a Stem Cell Based Gene Therapy Tumor Model. ,
J Vis Exp
 70 , e4181 , 2012
Cells
Liver hematopoietic
Enzymes
Medium
RPMI
Liver
Human
Human
Torok, E. , Lutgehetmann, M. , Bierwolf, J. , Melbeck, S. , Dullmann, J. , Nashan, B. , Ma, P. and Pollok, J.
Primary Human Hepatocytes on Biodegradable Poly(l-lactic acid) Matrices: A Promising Model for Improving Transplantation Efficiency with Tissue Engineering. ,
Liver Transpl
 17 , 104 , 2011
Cells
Hepatocytes
Enzymes
Medium
Williams E
Liver
Human
Human
Wauthier, E. , Schmelzer, E. , Turner, W. , Zhang, L. , LeCluyse, E. , Ruiz, J. , Turner, R. , Furth, M. , Kubota, H. , Lozoya, O. , Barbier, C. , McClelland, R. , Yao, H. , Moss, N. , Bruce, A. , Ludlow, J. and Reid, L.
Hepatic Stem Cells and Hepatoblasts: Identification, Isolation, and Ex Vivo Maintenance. ,
Methods Cell Biol
 86 , 137 , 2008
Cells
Hepatic stem cells and heptoblasts
Enzymes
Collagenase Type 4 : 0.014-0.06%
Medium
various
Liver
Human
Human
Pichard L, Raulet E, Fabre G, Ferrini JB, Ourlin JC. and Maurel P
Human hepatocyte culture ,
Methods Mol Biol
 320 , 283 , 2006
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
HEPES

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.