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Adipose/Fat
Bovine

Belgian Blue, 1-7 year

Mitić, R. , Cantoni, F. , Börlin, C. , Post, M. and Jackisch, L.
A Simplified and Defined Serum-free Medium for Cultivating Fat Across Species. , iScience Vol. 26 , 105822 , 2023
Cells
Stomal vascular cells
Medium
Growth medium
Adipose/Fat
Bovine
Bovine
Yang, Y. and Baldwin, R.
Preparation and Metabolism of Isolated Cells from Bovine Adipose Tissue ,
J Dairy Sci
 56 , 350 , 1973
Cells
Adipocytes
Enzymes
Collagenase Type 1 : 40 u/ml
Medium
Krebs-Ringer bicarbonate
Adrenal
Bovine
Bovine
Dominguez, N. , Rodri­guez, M. , Machado, J. and Borges, R.
Preparation and Culture of Adrenal Chromaffin Cells. ,
Methods Mol Biol
 846 , 223 , 2012
Cells
Chromaffin
Enzymes
Collagenase Type 1 : 0.1-0.2%
Deoxyribonuclease I : 0.003-0.015%
Hyaluronidase : 0.015%
Medium
DMEM/F12
Adrenal
Bovine
Bovine, 6 month
Moustafa T, Girod S, Tortosa F, Li R, Sol JC, Rodriguez F, Bastide R, Lazorthes Y, Sallerin B.
Viability and functionality of bovine chromaffin cells encapsulated into alginate-PLL microcapsules with a liquefied inner core ,
Cell Transplant
 15 , 121-33 , 2006
Cells
Chromaffin
Enzymes
Medium
Locke's solution
Adrenal
Bovine
Bovine
Hahm, S. , Chen, Y. , Vinson, C. and Eiden, L.
A Calcium-Initiated Signaling Pathway Propagated Through Calcineurin and cAMP Response Element-Binding Protein Activates Proenkephalin Gene Transcription after Depolarization. ,
Mol Pharmacol
 64 , 1503 , 2003
Cells
Chromaffin cells
Enzymes
Collagenase : 0.1%
Medium
DMEM
Adrenal
Bovine
Bovine
Ortega, J. , Sagen, J. and Pappas, G.
Short-term Immunosuppression Enhances Long-term Survival of Bovine Chromaffin Cell Xenografts in Rat CNS ,
Cell Transplant
 1 , 33 , 1992
Cells
Chromaffin
Enzymes
Collagenase : 0.2%
Medium
Locke's solution
Adrenal
Bovine
Bovine
Dahmer, M. , Hart, P. and Perlman, R.
Studies on the Effect of Insulin-Like Growth Factor-I on Catecholamine Secretion from Chromaffin Cells ,
J Neurochem
 54 (3) , 931 , 1990
Cells
Chromaffin
Enzymes
Medium
DMEM
Adrenal
Bovine
Bovine
Higgins, L. and Berg, D.
Immunological Identification of a Nicotinic Acetylcholine Receptor on Bovine Chromaffin Cells ,
J Neurosci
 7 (6) , 1792 , 1987
Cells
Chromaffin
Enzymes
Medium
Locke's solution
Adrenal
Bovine
Bovine
Aunis, D. , Rotllan, P. and Miras-Portugal, M.
Incorporation of Adenosine into Nucleotides of Chromaffin Cells in Culture ,
Neurochem Int
 7 , 89 , 1985
Cells
Chromaffin
Enzymes
Collagenase : 0.05%
Medium
Locke's solution, CMF
Adrenal
Bovine
Bovine
Almazan, G. , Aunis, D. , Garcia, A. , Montiel, C. , Nicolas, G. and Sanchez-Garcia, P.
Effects Of CLS on the Release of Noradrenaline From Chromaffin Cells ,
Br J Biomed Sci
 81 , 599 , 1984
Cells
Chromaffin
Enzymes
Collagenase : 0.15%
Medium
Kreb's, CMF
Adrenal
Bovine
Bovine
Pollard, H. , Pazoles, C. , Creutz, C. , Scott, J. , Zinder, O. and Hotchkiss, A.
An Osmotic Mechanism For Exocytosis From Dissociated Chromaffin Cells ,
J Biol Chem
 259 , 1114 , 1984
Cells
Chromaffin
Enzymes
Collagenase : 0.1%
Medium
(see reference)
Adrenal
Bovine
Bovine
Cena, V. , Garcia, A. , Montiel, C. and Sanchez-Garcia, P.
Uptake of [3H]-nicotine and [3H]-noradrenaline by Cultured Chromaffin Cells ,
Br J Pharmacol
 81 , 119 , 1984
Cells
Chromaffin
Enzymes
Medium
CF Kreb's
Adrenal
Bovine
Bovine
Waymire, J. , Bennett, W. , Boehme, R. , Hankins, L. , Gilmer-Waymire, K. and Haycock, J.
Bovine Adrenal Chromaffin Cells: High-Yield Purification and Viability in Suspension Culture ,
J Neurosci Methods
 7 , 329 , 1983
Cells
Chromaffin
Enzymes
Medium
HBSS, modified
Adrenal
Bovine
Bovine
Greenberg, A. and Zinder, O.
alpha- and beta-Receptor Control of Catecholamine Secretion from Isolated Adrenal Medulla Cells ,
Cell Tissue Res
 226 , 655 , 1982
Cells
Medulla
Enzymes
Collagenase : 0.2%
Medium
Krebs-Ringer bicarbonate buffer, CMF
Adrenal
Bovine
Bovine
Kilpatrick, D. , Ledbetter, F. , Carson, K. , Kirshner, A. , Slepetis, R. and Kirshner, N.
Stability of Bovine Adrenal Medulla Cells in Culture ,
J Neurochem
 35 (3) , 679 , 1980
Cells
Medulla
Enzymes
Collagenase : 0.05%
Medium
Locke's solution, CF
Adrenal
Bovine
Bovine
Aunis, D. , Guerold, B. , Bader, M-F. and Cieselski-Treska, J.
Immunocytochemical and Biochemical Demonstration of Contractile Proteins in Chromaffin Cells ,
Neuroscience
 5 , 2261 , 1980
Cells
Chromaffin
Enzymes
Collagenase : 0.05%
Medium
DMEM
Adrenal
Bovine
Bovine
Trifaro, J.M. and Lee, R.W.
Morphological Characteristics and Stimulus-Secretion Coupling in Bovine Adrenal Chromaffin Cell Cultures ,
Neuroscience
 5 , 1533 , 1980
Cells
Chromaffin
Enzymes
Collagenase : 0.05%
Medium
Locke's solution, CMF
Adrenal
Bovine
Bovine, adult
(also rat, Hanover-Wistar, young; guinea-pig, newborn)
Unsicker, K. , Rieffert, B. and Ziegler, W.
Effects of Cell Culture Conditions, Nerve Growth Factor, Dexamethasone, and Cyclic AMP on Adrenal Chromaffin Cells In Vitro ,
Adv Biochem Psychopharmacol
 255 , 51 , 1980
Cells
Chromaffin
Enzymes
Collagenase : 0.5%
Collagenase : 0.5%
Medium
HBSS
Adrenal
Bovine
Bovine
Kumakura, K. , Karoum, F. , Guidotti, A. and Costa, E.
Modulation of Nicotinic Receptors by Opiate Receptor Agonists in Cultured Adrenal Chromaffin Cells ,
Nature
 283 , 489 , 1980
Cells
Chromaffin
Enzymes
Collagenase : 0.25%
Medium
F-12 medium
Adrenal
Bovine
Bovine, adult
Unsicker, K. and Griesser, G.
Establishment, Characterization and Fibre Outgrowth of Isolated Bovine Adrenal Medullary Cells in Long-Term Cultures ,
Neuroscience
 5 , 1445 , 1980
Cells
Medulla
Enzymes
Medium
HBSS
Adrenal
Bovine
Bovine
Folkman, J. , Haudenschild, C. C. and Zetter, B. R.
Long-term Culture of Capillary Endothlial Cells ,
Proc Natl Acad Sci U S A
 76 , 5217 , 1979
Cells
Foreskin
Enzymes
Collagenase : 0.5%
Medium
Dulbecco's MEM w/10% calf serum
Adrenal
Bovine
Bovine
Hersey, R. and DiStefano, V.
Control of Phenylethanolamine N-Methyltransferase by Glucocorticoids in Cultured Bovine Adrenal Medullary Cells ,
J Pharmacol Exp Ther
 209 (1) , 147 , 1979
Cells
Medullary
Enzymes
Medium
HEPES
Adrenal
Bovine
Bovine
Fenwick, E. , Fajdiga, P. , Howe, N. and Livett, B.
Functional and Morphological Characterization of Isolated Bovine Adrenal Medullary Cell ,
J Cell Biol
 76 , 12 , 1978
Cells
Medulla
Enzymes
Medium
Kreb's, CF
Adrenal
Bovine
Bovine, male
Schneider, A. , Herz, R. and Rosenheck, K.
Stimulus-Secretion Coupling in Chromaffin Isolated From Adrenal Medulla ,
Proc Natl Acad Sci U S A
 74 , 5036 , 1977
Cells
Chromaffin
Enzymes
Medium
Kreb's Ringer bicarbonate buffer, CF
Bone
Bovine
Bovine
Buschmann, M. , Gluzband, Y. , Grodzinsky, A. and Hunziker, E.
Mechanical Compression Modulates Matrix Biosynthesis in Chondrocyte/Agarose Culture ,
J Cell Sci
 108 , 1497 , 1995
Cells
Chondrocytes
Enzymes
Medium
DMEM
Brain
Bovine
Bovine
Wolburg H, Neuhaus J, Kniesel U, Krauss B, Schmid EM, Ocalan M, Farrell C, Risau W.
Modulation of tight junction structure in blood-brain barrier endothelial cells. Effects of tissue culture, second messengers and cocultured astrocytes ,
J Cell Sci
 107 , 1347 , 1994
Cells
Brain endothelial cells
Enzymes
Medium
DMEM
Brain
Bovine
Bovine, adult
Machi, T. , Kassell, N.F. and Scheld, W.M.
Isolation and Characterization of Endothelial Cells From Bovine Cerebral Arteries ,
In Vitro Cell Dev Biol
 26 , 291 , 1990
Cells
Cerebral artery
Endothelial
Enzymes
Collagenase : 0.2%
Medium
HBSS
Brain
Bovine
Bovine
Estrada, C. , Bready, J. , Berliner, J. and Cancilla, P.
Choline Uptake by Cerebral Capillary Endothelial Cells in Culture ,
J Neurochem
 54 , 1467 , 1990
Cells
Capillary endothelial
Enzymes
Collagenase : 0.1%
Medium
DMEM
Brain
Bovine
Bovine
Goetz, I.. , Warren, J. , Estrada, C. , Roberts, E. and Krause, D.
Long-Term Cultivation of Arterial and Capillary Endothelium From Adult Bovine Brain ,
In Vitro Cell Dev Biol
 21 , 172 , 1985
Cells
Endothelial
Brain arteries
Enzymes
Medium
Dulbecco's PBS
Cartilage
Bovine
Bovine, 18-36 month
White, R. and Gibson, J.
The Effect of Oxygen Tension on Calcium Homeostasis in Bovine Articular Chondrocytes. ,
J Orthop Surg Res
 5 , 27 , 2010
Cells
Chondrocytes
Enzymes
Medium
DMEM
Cartilage
Bovine
Bovine, 6-8 week
Hwang, Y. , Sangaj, N. and Varghese, S.
Interconnected Macroporous Poly(ethylene glycol) Cryogels as a Cell Scaffold for Cartilage Tissue Engineering. ,
Tissue Eng Part A
 16 , 3033-41 , 2010
Cells
Chondrocytes
Enzymes
Medium
DMEM
Cartilage
Bovine
Bovine, 1-3 month
Lima, E. , Tan, A. , Tai, T. , Marra, K. , DeFail, A. , Ateshian, G. and Hung, C.
Genipin Enhances the Mechanical Properties of Tissue-Engineered Cartilage and Protects Against Inflammatory Degradation When Used as a Medium Supplement. ,
J Biomed Mater Res
 91 , 692 , 2009
Cells
Chondrocytes
Enzymes
Collagenase Type 4 : 390 u/ml
Medium
DMEM
Cartilage
Bovine
Calf, 1-14 days
Klagsbrun, M.
Large Scale Preparation of Chondrocytes , Methods of Enzymology Vol. 58 , 560 , 1979
Cells
Chondrocytes
Enzymes
Medium
PBS
Cartilage
Goat

Goat

Burdis, R. , Chariyev-Prinz, F. , Browe, D. , Freeman, F. , Nulty, J. , McDonnell, E. , Eichholz, K. , Wang, B. , Brama, P. and Kelly, D.
Spatial Patterning of Phenotypically Distinct Microtissues to Engineer Osteochondral Grafts for Biological Joint Resurfacing. ,
Biomaterials
 289 , 121750 , 2022
Cells
Chondrocytes
Enzymes
Collagenase Type 2 : 350 u/ml
Medium
DMEM
Cartilage
Goat
Goat
Bekkers,J, Creemers, L, Tsuchida, A, van Rijen, M, Custers, R, Dhert, W. and Saris, D.
One-Stage Focal Cartilage Defect Treatment with Bone Marrow Mononuclear Cells and Chondrocytes Leads to Better Macroscopic Cartilage Regeneration Compared to Microfracture in Goats. ,
Osteoarthritis Cartilage
 21 , 950-6 , 2013
Cells
Chondrocytes
Enzymes
Medium
DMEM
Endothelial
Bovine
Bovine
Ricken Albert M, Traenkner Anja, Merkwitz Claudia, Hummitzsch Katja, Grosche Jens, Spanel-Borowski Katharina.
The short prolactin receptor predominates in endothelial cells of micro- and macrovascular origin ,
J Vasc Res
 44 , 19-30 , 2007
Cells
Bovine umbilical cord (BUVEC)
Enzymes
Collagenase : 0.1%
Medium
Dulbecco's/Ham F-12

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.