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Tissue References Search Tool
Human
Human
An Optimized Dissagregation Method for Human Lung Tumors That Perserves the Phenotype and Function of the Immune Cells
,
J Leukoc Biol
97
, 201
, 2015
Cells
Lung tumor
Enzymes
Collagenase Type 1
: 45-60 u/ml
Collagenase Type 2
: 15-20 u/ml
Collagenase Type 4
: 45-60 u/ml
Elastase
: 0.002%
Deoxyribonuclease I
: 0.002%
Medium
DMEM/F12
Mouse
Mouse, male
Isolation of Immune Cells from Primary Tumors.
,
J Vis Exp
64
, e3952
, 2012
Cells
Dendritic, macrophages
Enzymes
Collagenase (1 or 4)
: 100-200 u/ml
Deoxyribonuclease I
: 0.01%
Medium
RPMI