Tissue References Search Tool

Mouse

Mouse

Comeau, K. , Caillon, A. , Paradis, P. and Schiffrin, E.

Determination of Interleukin-17A and Interferon-γ Production in γδ, CD4, and CD8 T Cells Isolated from Murine Lymphoid Organs, Perivascular Adipose Tissue, Kidney, and Lung. ,
Bio Protoc
13 , e4679 , 2023

Cells

T cells

Enzymes

Collagenase Type 1 : 0.1%

Collagenase Type 2 : 0.2%

Elastase : 2 u/ml

Soybean Trypsin Inhibitor : 0.025%

Hyaluronidase : 50 u/ml

Medium

RPMI

Mouse

Mouse

Villarreal, E. , Hewgley, W. , Lang, W. , Morton, C. , Mao, S. , Wu, J. and Sandoval, J.

In-bag Enzymatic Splenic Digestion: A Novel Alternative to Manual Morcellation? ,
J Surg Res
218 , 209-216 , 2017

Cells

Speen

Enzymes

Collagenase Type 5 : 5%

Hyaluronidase : 2.5%

Elastase : 1.25%

Medium

HBSS

Human

Human

Quatromoni, J. , Singhal, S. , Bhojnagarwala, P. , Hancock, W. , Albelda, S. and Eruslanov, E.

An Optimized Dissagregation Method for Human Lung Tumors That Perserves the Phenotype and Function of the Immune Cells ,
J Leukoc Biol
97 , 201 , 2015

Cells

Lung tumor

Enzymes

Collagenase Type 1 : 45-60 u/ml

Collagenase Type 2 : 15-20 u/ml

Collagenase Type 4 : 45-60 u/ml

Elastase : 0.002%

Deoxyribonuclease I : 0.002%

Medium

DMEM/F12

Tissue Tables (references, grouped by tissue type and species)

Thyroid/Parathyroid