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Tissue References Search Tool

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Neural
Rat
Rat, Wistar, 4 day
Obradovic Darja, Gronemeyer Hinrich, Lutz Beat, Rein Theo.
Cross-talk of vitamin D and glucocorticoids in hippocampal cells ,
J Neurochem
 96 , 500-9 , 2006
Cells
Hippocampal neurons
Enzymes
PDS kit : per instructions
Medium
Neurobasal A
Neural
Rat
Rat, SD, neonatal
Lacroix-Fralish, M. , Tawfik, V. , Nutile-McMenemy, N. , Harris, B. and Deleo, J.
Differential Regulation of Neuregulin 1 Expression by Progesterone in Astrocytes and Neurons. ,
Neuron Glia Biol
 2 , 227 , 2006
Cells
astrocytes
Enzymes
PDS kit : per instructions
Medium
DMEM
Neural
Rat
Rat, 2 month
Mothe, A. , Kulbatski, I. , Van Bendegem, R. , Lee, L. , Kobayashi, E. , Keating, A. and Tator, C.
Analysis of Green Fluorescent Protein Expression in Transgenic Rats for Tracking Transplanted Neural Stem/Progenitor Cells ,
J Histochem Cytochem
 53(10) , 1215 , 2005
Cells
Spinal cord progenitor cells
Hybrid toxins
Enzymes
PDS kit : see reference
Medium
Neurobasal A
Neural
Rat
Rat, SD, E19
Gavva NR, Tamir R, Qu Y, Klionsky L, Zhang TJ, Immke D, Wang J, Zhu D, Vanderah TW, Porreca F, Doherty EM, Norman MH, Wild KD, Bannon AW, Louis JC, Treanor JJ.
AMG 9810 [(E)-3-(4-t-butylphenyl)-N-(2,3-dihydrobenzo[b][1,4] dioxin-6-yl)acrylamide], a novel vanilloid receptor 1 (TRPV1) antagonist with antihyperalgesic properties ,
J Pharmacol Exp Ther
 313 , 474 , 2005
Cells
Dorsal root ganglia
Enzymes
PDS kit : per instructions
Medium
MEM/Ham's F12
Neural
Rat
Rat, fetal
Lovshin JA, Huang Q, Seaberg R, Brubaker PL, Drucker DJ.
Extrahypothalamic expression of the glucagon-like peptide-2 receptor is coupled to reduction of glutamate-induced cell death in cultured hippocampal cells ,
Endocrinology
 145 , 3495 , 2004
Cells
Brainstem and cortical neurons
Enzymes
PDS kit : per instructions
Medium
DMEM
Neural
Rat
Rat, SD, male, 270-330 g
Lin CR, Wu PC, Shih HC, Cheng JT, Lu CY, Chou AK, Yang LC.
Intrathecal spinal progenitor cell transplantation for the treatment of neuropathic pain ,
Cell Transplant
 11 , 17 , 2002
Cells
Spinal progenitor cells
Enzymes
PDS kit : with modifications
Medium
Neurobasal medium
Neural
Rat
Rat, embryonic 18-19 day
Mabuchi T, Kitagawa K, Kuwabara K, Takasawa K, Ohtsuki T, Xia Z, Storm D, Yanagihara T, Hori M, Matsumoto M.
Phosphorylation of cAMP response element-binding protein in hippocampal neurons as a protective response after exposure to glutamate in vitro and ischemia in vivo ,
J Neurosci
 21 , 9204-13 , 2001
Cells
Hippocampal neurons
Enzymes
PDS kit : see reference
Medium
DMEM
Organoids
Human

Human

Tresenrider, A. , Sridhar, A. , Eldred, K. , Cuschieri, S. , Hoffer, D. , Trapnell, C. and Reh, T.
Single-cell Sequencing of Individual Retinal Organoids Reveals Determinants of Cell Fate Heterogeneity. , bioRxiv , 2023
Cells
Retinal Organoids
Enzymes
PDS kit : see reference
Medium
DMEM
Organoids
Human

Human

Schröter, M. , Wang, C. , Terrigno, M. , Hornauer, P. , Huang, Z. , Jagasia, R. and Hierlemann, A.
Functional Imaging of Brain Organoids Using High-Density Microelectrode Arrays. , MRS Bull Vol. 47 , 530-544 , 2022
Cells
Cerebral organoids
Enzymes
PDS kit : see reference
Medium
PBS
Organoids
Human

Human

Uzquiano, A. , Kedaigle, A. , Pigoni, M. , Paulsen, B. , Adiconis, X. , Kim, K. , Faits, T. , Nagaraja, S. , Antón-Bolaños, N. , Gerhardinger, C. , Tucewicz, A. , Murray, E. , Jin, X. , Buenrostro, J. , Chen, F. , Velasco, S. , Regev, A. , Levin, J. and Arlotta, P.
Proper Acquisition of Cell Class Identity in Organoids Allows Definition of Fate Specification Programs of the Human Cerebral Cortex ,
Cell
 185 , 3770-3788.e27 , 2022
Cells
Brain organoids
Enzymes
PDS kit : see reference
Medium
EBSS
Organoids
Human

Human

Xue, Y. , Seiler, M. , Tang, W. , Wang, J. , Delgado, J. , McLelland, B. , Nistor, G. , Keirstead, H. and Browne, A.
Retinal Organoids On-a-Chip: a Micro-Millifluidic Bioreactor for Long-term Organoid Maintenance. ,
Lab Chip
 21 , 3361-3377 , 2021
Cells
Stem cell-derived retinal organoids
Enzymes
PDS kit : see reference
Medium
EBSS
Organoids
Human

Human

Vaart, J. , Böttinger, L. , Geurts, M. , Wetering, W. , Knoops, K. , Sachs, N. , Begthel, H. , Korving, J. , Lopez-Iglesias, C. , Peters, P. , Eitan, K. , Gileles-Hillel, A. and Clevers, H.
Modelling of Primary Ciliary Dyskinesia using Patient-derived Airway Organoids. , EMBO Rep Vol. 22 , e52058 , 2021
Cells
Airway organoids
Enzymes
Pronase : 0.15%
Collagenase : 0.05%
Medium
Airway medium
Organoids
Human

Human

Barbar, L. , Rusielewicz, T. , Zimmer, M. , Kalpana, K. and Fossati, V.
Isolation of Human CD49f Astrocytes and iPSC-Based Neurotoxicity Assays. , STAR Protoc Vol. 1 , 100172 , 2020
Cells
Astrocytes
Enzymes
PDS kit : see reference
Medium
EBSS
Organoids
Human

Human

Velasco, S. , Kedaigle, A. , Simmons, S. , Nash, A. , Rocha, M. , Quadrato, G. , Paulsen, B. , Nguyen, L. , Adiconis, X. , Regev, A. , Levin, J. and Arlotta, P.
Individual Brain Organoids Reproducibly Form Cell Diversity of the Human Cerebral Cortex. ,
Nature
 570 , 523-527 , 2019
Cells
Brain organoids
Enzymes
PDS kit : see reference
Medium
PBS
Reproductive
Bovine
Bovine
(also porcine, human)
Marcus, G. , Connor, L. , Domingo, M. , Tsang, B. , Downey, B. and Ainsworth, L.
Enzymatic Dissociation of Ovarian and Uterine Tissues ,
Endocr Res
 10 , 151 , 1984
Cells
Interna & corpus luteum
Endometrium
Ovarian
Uterine
Enzymes
Pronase : 0.1%
Medium
Moscona's BSS
Reproductive
Human
Human
(also porcine, bovine)
Marcus, G. , Connor, L. , Domingo, M. , Tsang, B. , Downey, B. and Ainsworth, L.
Enzymatic Dissociation of Ovarian and Uterine Tissues ,
Endocr Res
 10 , 151 , 1984
Cells
Interna & corpus luteum
Endometrium
Ovarian
Uterine
Enzymes
Pronase : 0.1%
Medium
Moscona's BSS
Reproductive
Porcine
Porcine (also bovine, human)
Marcus, G. , Connor, L. , Domingo, M. , Tsang, B. , Downey, B. and Ainsworth, L.
Enzymatic Dissociation of Ovarian and Uterine Tissues ,
Endocr Res
 10 , 151 , 1984
Cells
Interna & corpus luteum
Endometrium
Ovarian
Uterine
Enzymes
Pronase : 0.1%
Medium
Moscona's BSS
Stem
Human
Human
Mothe, A. and Tator, C.
Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord. ,
J Vis Exp
 , e52732 , 2015
Cells
Spinal cord neural progenitor
Enzymes
PDS kit : with modifications
Medium
EBSS
Stem
Human
Human
Hjelmeland, A. , Wu, Q. , Wickman, S. , Eyler, C. , Heddleston, J. , Shi, Q. , Lathia, J. , Macswords, J. , Lee, J. , McLendon, R. and Rich, J.
Targeting A20 Decreases Glioma Stem Cell Survival and Tumor Growth. ,
PLoS Biol
 8 , e1000319 , 2010
Cells
Glioma stem cells
Enzymes
PDS kit : per instructions
Medium
neurobasal medium
Stem
Mouse
Mouse
Rountree, C. , Ding, W. , Dang, H. , Vankirk, C. and Crooks, G.
Isolation of CD133+ Liver Stem Cells for Clonal Expansion. ,
J Vis Exp
 56 , e3183 , 2011
Cells
CD133+ stem
Enzymes
Pronase : 0.05%
Medium
DMEM/F12
Stem
Mouse
Mouse
Klein C, Butt SJ, Machold RP, Johnson JE. and Fishell G.
Cerebellum- and forebrain-derived stem cells possess intrinsic regional character ,
Development
 132 , 4497 , 2005
Cells
Neurosperes
Enzymes
PDS kit : per instructions
Medium
DMEM/F12
Stem
Mouse
Mouse, 1 day
Seaberg, R. , Smukler, S. and Van der Kooy, D.
Intrinsic Differences Distinguish Transiently Neurogenic Progenitors from Neural Stem Cells in the Early Postnatal Brain. ,
Dev Biol
 278 , 71 , 2005
Cells
Neural progenitor
Enzymes
PDS kit : per instructions
Medium
see reference
Stem
Mouse
Mouse, embryonic
Estivill-Torrus, G. , Pearson, H. , Van Heyningen, V. , Price, D. and Rashbass, P.
Pax6 is Required to Regulate the Cell Cycle and the Rate of Progression from Symmetrical to Asymmetrical Division in Mammalian Cortical Progenitors. ,
Development
 129 , 455 , 2002
Cells
Cortical progenitors
Enzymes
PDS kit : per instructions
Medium
Serum free medium
Stem
Mouse
Mouse
Boulanger CA, Smith GH.
Reducing mammary cancer risk through premature stem cell senescence ,
Oncogene
 20 , 2264-72 , 2001
Cells
Mammary epithelial stem
Enzymes
Hyaluronidase : 0.1%
Pronase : 1.25%
Medium
DMEM
Stem
Rat
Rat, 6-8 week
Mothe, A. and Tator, C.
Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord. ,
J Vis Exp
 , e52732 , 2015
Cells
Spinal cord neural progenitor
Enzymes
PDS kit : with modifications
Medium
EBSS
Stem
Rat
Rat, 2 month
Mothe, A. , Kulbatski, I. , Van Bendegem, R. , Lee, L. , Kobayashi, E. , Keating, A. and Tator, C.
Analysis of Green Fluorescent Protein Expression in Transgenic Rats for Tracking Transplanted Neural Stem/Progenitor Cells ,
J Histochem Cytochem
 53(10) , 1215 , 2005
Cells
Spinal cord progenitor cells
Hybrid toxins
Enzymes
PDS kit : see reference
Medium
Neurobasal A
Tumor
Human
Human
Yan, K. , Wu, Q. , Yan, D. , Lee, C. , Rahim, N. , Tritschler, I. , DeVecchio, J. , Kalady, M. , Hjelmeland, A. and Rich, J.
Glioma Cancer Stem Cells Secrete Gremlin1 to Promote their Maintenance Within the Tumor Hierarchy. ,
Genes Dev
 28 , 1085-100 , 2014
Cells
Glioma cancer stem
Enzymes
PDS kit : per instructions
Medium
Neurobasal
Tumor
Mouse
Mouse
Boyd, N. , Walker, K. , Fried, J. , Hackney, J. , McDonald, P. , Benavides, G. , Spina, R. , Audia, A. , Scott, S. , Libby, C. , Tran, A. , Bevensee, M. , Griguer, C. , Nozell, S. , Gillespie, G. and Nabors, B.
Addition of Carbonic Anhydrase 9 Inhibitor SLC-0111 to Temozolomide Treatment Delays Glioblastoma Growth In Vivo. ,
JCI Insight
 2 , 2017
Cells
Glioblastoma
Enzymes
PDS kit : per instructions
Medium
DMEM/F12
Tumor
Mouse
Mouse
Otvos, B. , Silver, D. , Mulkearns-Hubert, E. , Alvarado, A. , Turaga, S. , Sorensen, M. , Rayman, P. , Flavahan, W. , Hale, J. , Stoltz, K. , Sinyuk, M. , Wu, Q. , Jarrar, A. , Kim, S. , Fox, P. , Nakano, I. and Rich, J.
Cancer Stem Cell-Secreted Macrophage Migration Inhibitory Factor Stimulates Myeloid Derived Suppressor Cell Function and Facilitates Glioblastoma Immune Evasion. ,
Stem Cells
 34 , 2026-39 , 2016
Cells
Glioblastoma
Enzymes
PDS kit : per instructions
Medium
Neurobasal

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.