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Tissue References Search Tool

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Epithelial
Canine
Dog
Virmani, A. , Naziruddin, B. , Desai, V. , Lowry, J. , Graves, D. and Sachdev, G.
Evidence for Secretion of High Molecular Weight Mucins by Canine Tracheal Epithelial Cells in Primary Culture: Effects of Select Secretagogues on Mucin Secretion ,
In Vitro Cell Dev Biol
 28A , 120 , 1992
Cells
Tracheal
Enzymes
Pronase : 0.1%
Medium
DMEM
Liver
Mouse
Mouse, 12 week
Mederacke, I. , Dapito, D. , Affo, S. , Uchinami, H. and Schwabe, R.
High-Yield and High-Purity Isolation of Hepatic Stellate Cells from Normal and Fibrotic Mouse Livers. ,
Nat Protoc
 10 , 305-15 , 2015
Cells
Hepatic stellate
Enzymes
Pronase : 0.04%
Medium
DMEM
Liver
Mouse
Mouse
Rountree, C. , Ding, W. , Dang, H. , Vankirk, C. and Crooks, G.
Isolation of CD133+ Liver Stem Cells for Clonal Expansion. ,
J Vis Exp
 56 , e3183 , 2011
Cells
CD133+ stem
Enzymes
Pronase : 0.05%
Medium
DMEM/F12
Mammary
Mouse
Mouse
Boulanger CA, Smith GH.
Reducing mammary cancer risk through premature stem cell senescence ,
Oncogene
 20 , 2264-72 , 2001
Cells
Mammary epithelial stem
Enzymes
Hyaluronidase : 0.1%
Pronase : 1.25%
Medium
DMEM
Mammary
Mouse
Mouse (BALB/cCrgl), mature
Bandyopadhyay, G. , Imagawa, W. , Wallace, D. and Nandi, S.
Proliferative effects of insulin and epidermal growth factor on mouse mammary epithelial cells in primary culture ,
J Biol Chem
 263 , 7567 , 1988
Cells
Epithelial
Enzymes
Pronase : 0.01%
Medium
Hepes buffered medium 199
Miscellaneous
Canine

Canine, Rat, Rabbit, Ovine, Bovine, Human

Basatvat, S. , Bach, F. , Barcellona, M. , Binch, A. , Buckley, C. , Bueno, B. , Chahine, N. , Chee, A. and Maitre, C.
Harmonization and Standardization of Nucleus Pulposus Cell Extraction and Culture Methods. ,
Spine
 6 , e1238 , 2023
Cells
nucleus pulposus
Enzymes
Pronase : 0.075%
Medium
DMEM
Muscle
Mouse
Mouse, 10 week
Rezk, B. , Yoshida, T. , Semprun-Prieto, L. , Higashi, Y. , Sukhanov, S. and Delafontaine, P.
Angiotensin II Infusion Induces Marked Diaphragmatic Skeletal Muscle Atrophy. ,
PLoS ONE
 7 , e30276 , 2012
Cells
Diaphragm cells
Enzymes
Collagenase Type 2 : 100 u/ml
Pronase : 0.125%
Medium
PBS
Stem
Mouse
Mouse
Rountree, C. , Ding, W. , Dang, H. , Vankirk, C. and Crooks, G.
Isolation of CD133+ Liver Stem Cells for Clonal Expansion. ,
J Vis Exp
 56 , e3183 , 2011
Cells
CD133+ stem
Enzymes
Pronase : 0.05%
Medium
DMEM/F12
Stem
Mouse
Mouse
Boulanger CA, Smith GH.
Reducing mammary cancer risk through premature stem cell senescence ,
Oncogene
 20 , 2264-72 , 2001
Cells
Mammary epithelial stem
Enzymes
Hyaluronidase : 0.1%
Pronase : 1.25%
Medium
DMEM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.